Forensic DNA Laboratory of

INP
LABORATORIUM DNA FORENSIK
BIDDOKPOL PUSDOKKES POLRI
1990 2005
Dr. Christanto TH, dr. Slamet Poernomo, drg. Alphonsus Q, dr. Lukman Hakim as poineer of the DNA
laboratory at Biddokpol Pusdokkes Polri
Serology : pretest; Dot Blot (1992); Organic Isolation Validation (1993);HLA DQ-A(early 1994);
PolyMarker (mid 1994); D1S80 (late 1994); SA Gel Electrophoresis.
2006 March 26, 2007
Rehabilitation and development of the new DNA laboratory built under collaboration of INP and AFP,
on March 26, 2007 Kapolri and AFP Police Commissioner officially opened the new DNA laboratory
under the name
LABORATORIUM DNA FORENSIK BIDDOKPOL PUSDOKKES POLRI
March 2007 TODAY
Validation lab flow charts and standard operating procedures as well as the equipments and training
for human resources development program as part of the collaboration between INP AFP in
Canberra, Australia. Increasing the number of lab technicians with biologist or medical background.
Completion of SOP and manuals for the lab both in technical and quality terms, as part of the next
phase of the lab target, that is being internationally accredited, with the supervision and technical
support from AFP until 2009.
Photos of the new lab with the official
opening by kapolri & AFP Police
Commissioner
CE Room
Chemical &
Prep Room
Extraction Room Examination Room 2
Amp
Room
Pre Amp
Room
Examination
Room 1
Histology
Storage
Adminis
tration
II
nd
Flour DNA Lab Biddokpol Pusdokkes Polri
Server &
Power
Clothes
Flow of Samples
LABORATORIUM DNA FORENSIK
BIDDOKPOL PUSDOKKES POLRI
Bilateral Co-operation
1. The Eijkman Institute for Molecular Biology
2. Australian Federal Police
Regional Co-operations
ADNAP/REAFD
International Co-operations
ICPO/INTERPOL DNA Unit and DVI standing committee
LABORATORIUM DNA FORENSIK
BIDDOKPOL PUSDOKKES POLRI
Most of the cases we handled were criminal cases and some were family
disputes.
Types of cases:
Mutilation.
Murder / serial murder
Traces.
Paternity.
Missing person.
Terrorism.
Methods used for DNA extractions:
Chelex extraction for blood(dried blood from evidence or crime
scene, semen, buccal swabs, hair root and trace samples,
reference blood samples, soft tissue samples.
Organic Extraction for bone samples
victim
tools
suspect
Court
Crime scene
Paternity
DVI
W???
Fine out :
Apropriate sample
Apropriate
Another
cyrcle
32
LCN
28
Norma
Microcon
Genetic analyzer software
Use Population Database
Excel counting for Probability
All Equ.
All Procedures.
Pretest : Otol, AP.
Sampling : blood, swab, bone, hiroot, cig but, sperm, type-lift,
tooth, degraded.
Extraction : Chelex, organic.
Quant.
Amp.
Electrophoresis.
Typing.
Report.
fillingstoragewashingsterilewaterwasteworksh
eetsecurity MSDSfireemergency
aiddll...........................
INP-AFP-UC Scientists Training Course
University of Canberra
June 2007
Sub-population Abbr. N
Malay MT 100
Javanese JW 80
Javanese (Diabetic Patients) JV 20
North Sumatra (Batak Toba) TBS 20
North Sumatra (Batak Karo) KR 20
Mentawai MTW 20
Lembata LEM 20
East Flores (Larantuka) LAR 20
West Flores (Rampasasa) RAM 20
Central Flores SOA 20
Alor ALO 20
East Sumba (Sumba Timur) SBT 20
South Sulawesi (Toraja) TRJ 20
Kalimantan (Dayak-Benuaq) DY 20
N
INDIAN
OCEAN
PACIFIC
OCEAN
SOUTH
CHINA SEA
TIMOR
SEA
ARAFURA
SEA
PNG
AUSTRALIA
CELEBES
SEA
1. Central Flores (SOA)
2. West Flores (RAM)
3. East Flores (LAR)
4. Lembata (LEM)
5. Alor (ALO)
6. East Sumba (SBT)
7. South Sulawesi (TRJ)
8. Kalimantan (DY)
9. North Sumatra (KR)
10. North Sumatra (TBS)
11. Mentawai (MTW)
12. Javanese (JW)
13. Javanese (JV)
14. Malay
1 2 3,4
5
6
7
8
9
10
11
12,13,14
Neighbour-joining tree to represent full distance matrix
Javanese population departs from others and logical dispersion
of remaining groups on the basis of geography
MDS plot to represent full distance matrix. Populations that are
genetically close plot close together in 2-D space. Again,
Javanese population departs from the others whilst the
remaining groups tend towards clusters that have a
geographical basis.
Malay
JW
JV
DY
TRJ
MTW
TBS
KR
LEM
SBT
LAR
RAM
ALO
SOA
Nusa Tenggara
Nth West Sumatra,
Mentawai
Kalimantan, Java,
South Sulawesi
The source
Drive me to the reference forensic DNA lab.
The development of the science on DNA
polymorphism, its identification
technologies, and the related
biostatistics leads DNA to become an
almost perfect tool in the field of forensic
identification. It could be stated that DNA
use for personal identification is already
recognized and admissible worldwide
for judicial purposes.
Budi
Sampurna
DNA legislation
The use of DNA for judicial purposes in Indonesia has
been applied for more than one decade, particularly in
criminal and in disputed paternity cases.
When a mass disaster occurred in Bali in 2002 due to
bomb-explosions, DNA identification was applied and
contributed in 22.27% cases as a primary method
(n=202), while dental record was 55.44% and
fingerprint was 11.38%.
However, Indonesia doesnt have any legislation on the
application of DNA and its related science for judicial
purposes.
Budi
Sampurna
DNA legislation
The importance of DNA legislation in a country is to
regulate the application of DNA testing in order to
ensure and guarantee that it would be used properly
for the sake of peoples welfare and justice.
Legislation on DNA, at one side must be able to
regulate its optimal usage for delivering clinical
genetic services, law enforcement uses for forensic
purposes in both criminal and civil courts, insurance,
employment, sport, immigration and parentage and
kinship testing; and on the other side to protect
privacy, unfair discrimination and high ethical
standard
Budi
Sampurna
DNA legislation