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Genetic Engineering is possible, because We can cut DNA at specific sites by endonucleases like DNAase I the phenomenon of transduction in bacteria is well understood. The first transgenic animal was =Tobacco Pea Flax Cotton transgenic animals are those which are Foreign RNA in all its cell =Foreign DNA in some of its cells .
Genetic Engineering is possible, because We can cut DNA at specific sites by endonucleases like DNAase I the phenomenon of transduction in bacteria is well understood. The first transgenic animal was =Tobacco Pea Flax Cotton transgenic animals are those which are Foreign RNA in all its cell =Foreign DNA in some of its cells .
Genetic Engineering is possible, because We can cut DNA at specific sites by endonucleases like DNAase I the phenomenon of transduction in bacteria is well understood. The first transgenic animal was =Tobacco Pea Flax Cotton transgenic animals are those which are Foreign RNA in all its cell =Foreign DNA in some of its cells .
~We can cut DNA at specific sites by endonucleases like DNAase I
=Restriction endonucleases purified from bacteria can be used in vitro ~The phenomenon of transduction in bacteria is well understood ~We can see DNA by electron microscope } { Restriction endonucleases (Restriction enzymes) are used for Genetic Engineering ~Used to invitro DNA synthesis =Used in genetic engineering ~Synthesized by bacteria ~Present in mammalian cells for degradation of DNA } {Transgenic animals are those which are ~Foreign RNA in all its cell ~Foreign DNA in some of its cells =Foreign DNA in all its cells ~Both a and b } {The first transgenic plant was =Tobacco ~Pea ~Flax ~Cotton } {Which of the following is related to genetic Engineering? ~Plastid =Plasmid ~Heterosis ~Mutation } {First cloned animal =Dolly sheep ~Dog ~Mule ~Cat } {Which of the following bases in plasmids discovered so far ~50 kilo base =500 kilo base ~5 kilo base ~500 kilo base } {Plasmid has been used as vector because plasmid ~It has antibiotic resistance gene =It is circular DNA which have capacity to join to eukaryotic DNA ~Both ends show replication ~It can move between prokaryotic and eukaryotic cells } {The bacteria generally used for genetic engineering is ~Bacillus ~Pseudomonas ~Clostridium =Agrobacterium } {Which of the following cut the DNA from specific places =E.coli restriction endonuclease II ~Ligase ~Exonuclease ~Alkaline phosphatase } { Cosmids can carry genomic fragement upto.. ~ 30kb ~12kb = 45kb ~100kb } { The length of human genome is around.. ~ 200MB ~ 1000MB =3000MB ~500MB } { What is the role of DNA ligase ? ~cleavage of the DNA fragement ~ synthesis of DNA fragment =Joining of two ends of DNA fragement ~ All of the above } { Vent is an example of . ~ RNAse ~DNAse ~ Restriction Enzyme =Taq polymerase } { Taq polymerase is isolated from which bacterial species =Thermus aquanticus ~Bacillus substlis ~Aspergillus niger ~Penicillum noctatum } { Significance of Real time PCR =Quantification of DNA can be determined in each step ~Used for amplification of c-DNA ~ used for amplification of m-RNA ~all of the above } { pUC contains which marker gene ~leuZ =LacZ ~Ter ~All of the above } { Size of pBR322 is around ~2kb ~4kb =4.8kb ~6kb } { GFP stands for = Green fluorescent protein ~ Green florochrome protein ~Green fusion protein ~ all of the above } { The DNA shows maximum absorbance at .. ~230nm ~280nm =260nm ~300nm } { Screening strategy used for pUC cloning vector is ~red white selection ~blue black selection =blue white screening ~ none of the above } { EcoR1 produces =Sticky ~blunt ~both ~none of the above } { What is nature of lamda phages =lysogenic ~lytic ~both 1 &2 ~none of the above } { Single standed DNA can be cloned using which phage vector ~Lamda = M13 ~phagemid ~cosmid } { Maximum size of fragement to be cloned using plasmid. ~10kb ~20kb =5kb ~100kb } { Which enzyme can convert m-RNA to c-DNA =Reverse Transcriptase
~RNA Polymerase ~Restriction endonuclease ~ all of the above }