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Microbiology Revision. By dr. AboRashad Email .dr_alganzory@hotmail.com Mob.0598092704



90- A specific test for staphylococcus aureus is
a) Catalase test
b) Coagulase test
c) Optochin sensitivity test
d) Bacitracin sensitivity test
e) CAMP test

91- Bacteria that need dark field microscope to detected
a) T.pallidium
b) Klebsiella
c) Streptocoocus pyogenes
d) Colistridia sp

92-Autoclave sterilize in temp:
a) 121 c-20min
b) 125-35min
c) 115-25min
d) 160c-45-60 min

93-Strict aerobe bacteria
a) Mycobacterium
b) Klebsiella
c) Streptococcus pyogenes
d) Colistridia sp.

94-Citrate test detect:
a) Mycobacterium
b) Klebsiella
c) Streptococcus pyogenes
d) Colistridium sp

95-Capsulated bacteria
a) Mycobacterium
b) Klebsiella
c) Streptococcus pyogenes
d) Colistridium sp

96- Growth of vibrio cholerae on TCBS medium produces colonies
a) Yellow colonies
b) Blue colonies
c) Green colonies
d) White colonies
e) Red colonies

97- Organism cause bloody stool:
a) Shigella sp.
b) E.coli
c) Salmonella
d) Cambylobacter
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Microbiology Revision. By dr. AboRashad Email .dr_alganzory@hotmail.com Mob.0598092704



98-Media use for differentiate between L.F and N.L.F:
a) MacConkey agar
b) Chocolate
c) Blood agar
d) XLD
e) TCBS

99- Bacteriological media can be sterilized in:-
a) Oven
b) Filtration
c) Autoclave
d) Bunsen burner

100- All of the following are true about agar except:-
a) agar is inert(Non nutritive)
b) Nutritious
c) Solidity at 42c
d) Melts at 100c

101- The IMVIC Reaction of E. coli is
a) + + - -
b) - - + +
c) + + + -
d) -- + --

102- On DCA and maCconkey agar the salmonella/shigella give small
colonies, which colour of the following, appear
a) Black
b) pink
c) Brown
d) Pale yellow
e) Yellow-brown

103-In manual white blood cell count using haemocytometer the
dilution used is:
a) Normal saline
b) Sodium chloride
c) Glacial acetic acid
d) Water

104-Which of the following are sterilized in the oven:-
a) Media
b) Sand
c) Water
d) Blood
e) All of the above
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Microbiology Revision. By dr. AboRashad Email .dr_alganzory@hotmail.com Mob.0598092704

105-A parasite which does not deposit eggs in the intestine:
a) Entrobius.vermicularis
b) Anchylostoma. Duodenale
c) Ascaris lumbricoides
d) T.saginata

106- Specimens from a polio victim for culture can be best made on
a) Blood
b) Urine
c) Feces
d) CSF
e) Throat / Nasal washing

107- Beta hemolytic is enhanced when group B streptococci are
streaked at an angle in blood agar plate with:-
a) Streptococci
b) Staph aureus
c) micrococcus
d) Streptococcus epiedermidis
e) Corynebacterim diphteriae

108- To obtain an isotonic media, sodium chloride content should be:-
a) 0.58 g %
b) 0.085 g%
c) 8.5 g%
d) 6.5 g%
e) 0.50 g%

109- Group B beta-hemolytic streptococci are differentiated by other
streptococcus groups by using:-
a) Optochine
b) CAMP
c) Niacin test
d) Catalase test.
e) Bacitracin sensitivity disc

110- Most of pathogenic strains of streptococcus belongs to group :-
a) Viridans
b) Beta-hemolytic
c) Alpha-hemolytic (pneumonia)
d) Group d
e) Micrococcaciae






19
Microbiology Revision. By dr. AboRashad Email .dr_alganzory@hotmail.com Mob.0598092704

111- The trophozoite , which contains red blood cells in the
cytoplasm , brings to this parasite:-
a) Entamoeba histolytica
b) Entamoeba gingivalis
c) Entamoeba coli
d) Iodamoeba butschlii
e) None of the above

112- On EMB, the colour of the colonies of shigella salmonella is:-
a) Black
b) Green
c) Pink
d) Yellow
e) Colorless

113- Micro-organisms that grow at 60 c are classified as:-
a) Psychophysics
b) Mesosphilic
c) Hemophilic
d) Thermophilic
e) Saprophilic

114- A diagnostic finding for giardia lambilia in stool:-
a) Cyst in stool
b) Cyst and trophozoite in stool
c) Characteristic ova
d) Trophozoite in stool
e) All of the above

115- The concentration of agar in the culture medium is:-
a) 10 11 %
b) 20 %
c) 50 %
d) 60 %
e) 1-2 %

116- Best for identification of parasite if you can not see it in stool
(negative in stool)
a) Concentration technique
b) Flotation technique
c) Wet mount technique
d) Kato technique

117- Streptococcus pneumonia are :
a) Beta hemolytic
b) alpha hemolytic
c) No hemolytic

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Microbiology Revision. By dr. AboRashad Email .dr_alganzory@hotmail.com Mob.0598092704


118- Some organisms are to said to be pathogenic if they are
containing the following features:-
a) Coagulase
b) Catalase
c) Sugar
d) Antibodies

119- Organism soluble the bile:-
a) Staph.aureus
b) Streptococci
c) Pneumococci (streptococcus pneumonia)
d) Haemophilous influenzae

120- Organism that exhibits satellitism along with staph aureus:-
a) Haemophilus influenzae
b) Shigella sp.
c) Clostiridium sp.
d) S.pneumoniae

121- To differentiate between nisseria gonorrhoea & nisseria
meningitides
a) dextrose + lactose
b) dextrose + maltose
c) Glucose + Maltose
d) dextrose + Fructose

122- which of the following organisms is an anaerobic bacterium:-
a) Clostiridium spp.
b) Haemophilus
c) E.coli
d) Yersinia enterocolitica

123- What practical Cardiolipin is applicable in laboratory work?
a) FTA
b) VDRL&RPR
c) RPR only
d) VDRL only

124- Vi Ag is seen in:-
a) Capsule
b) Pili
c) Spore
d) Flagella




21
Microbiology Revision. By dr. AboRashad Email .dr_alganzory@hotmail.com Mob.0598092704

125- Purine associated with
a) Adenine
b) Guanine
c) Uric acid
d) RNA

126- To Clean the lenses of microscope we used
a) Water
b) Ethyl alcohol
c) Xylene
d) Chloroform
e) HCL

127- The determination of ASO titer is used in the laboratory
diagnosis of:-
a) Toxic shock syndrome
b) Scarlet fever
c) Streptococcal pharyngitis and cellulitis
d) Rheumatic fever

128-Which of the following Enlarges RBCs
a) Plasmodium vivax
b) Plasmodium malariae
c) Plasmodium falciprum
d) All of the above

129-Which of the following neither lays eggs nor deposits larvae in
the intestinal canal:-
a) Hookworms
b) Pinworm (Entrobius vermicularis)
c) Trichinella spiralis
d) Trichuris trichura

130-Identified by finding mainly the Rhabditiform larvae in in fresh
stool:-
a) Ascaris lumbricoides
b) Entrobius vermicularis
c) Strongyloides stercoralis
d) Trichinella spiralis

131-The resolution of the bright field microscope is:-
a) 2.0 um
b) 0.2 um
c) 0.02um
d) 0.002um


22
Microbiology Revision. By dr. AboRashad Email .dr_alganzory@hotmail.com Mob.0598092704

132-When using a 10 x eye piece and a 10 x objective of a bright field
microscope, the total magnification will be:-
a) 10
b) 100
c) 1000
d) 10000

133-For blood culture the best anticoagulant is
a) Citrate
b) Oxalate
c) Sodium polyanesthol sulphonate(SPS)
d) Heparin
e) EDITA

134-Hydated disease caused by
a) Trichomonas vaginalis
b) Tainia saginata
c) Echioccus granulosus

135-- Ancylostoma duodenalis diagnostic stage is
a) Embryonated egg
b) Cyst
c) Filariform larva
d) Trophozoit

136- Ancylostoma duodenalis Infective stage stage is
a) Embryonated egg
b) Cyst
c) Filariform larva
d) Trophozoit

137- Preservation of Urine Sample used
a) Acetic acid
b) Boric acid
c) Phenol
d) Formalin

138- The antibiotic disc which differentiate between group A
streptococci and other groups is?
a) Neomycin
b) Tetracycline
c) Bacitracin
d) CAMP
e) Optichine




23
Microbiology Revision. By dr. AboRashad Email .dr_alganzory@hotmail.com Mob.0598092704


139- The dwarf worm is?
a) Schistosoma sp.
b) Tenia saginata
c) H.nana
d) Tainia saginata

140- When focusing a stained smear under oil immersion field the
magnification is?
a) 10
b) 40
c) 100
d) 15

141- A standard micro plates in ELISA test has?
a) 98 wells
b) 94 wells
c) 96 wells
d) 48 wells

142- The enzyme in ELISA testing is present in the?
a) Buffer
b) Micro plate
c) Conjugate

143- The label in ELISA tests is?
a) Radio active substance
b) Antibody
c) Enzyme
d) Buffer
e)
) (
144- Best sterilization of Nutrient media done by:
a) Hot air oven
b) Autoclaving
c) Filtration
d) Radiation

H
145- H Ag present in:
a) Pilli
b) Capsule
c) Flagella
d) Cell membrane

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