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10 N8I - All + WarLhln-SLarry (splrocheLes)
+ Cll red C (faL)
+ Crlmellus (neuroendocrlne granules)
+ 8ouLlne flxaLlve
+ reservaLlon, general sLalnlng
+ lPC
+ Molecular Analysls
+ Long-Lerm sLorage
2 G|utara|dehyde - All - AS + LM
+ reservaLlon of collagen
- 8ouLlne flxaLlve
- Slow peneLraLlon
- needs refrldgeraLed
8S - Ln's
- Spleen
- 8M
+ CyLoplasmlc, nuclear sLalnlng
- 8ouLlne flxaLlve
- musL be prepared fresh
- requlres lodlne LreaLmenL Lo remove mercury before rouLlne
sLalnlng
- Llmed exposure needed because over flxaLlon cause of hardenlng
2enker's - All + Sheehan (chromaffln)
+ Mallory's 1AP (collagen & muscle)
+ vlral lncluslons (negrl bodles)
+ leulgen (unA)
+ 1rlchromes (collagen & muscle)
+ verhoeff-van Cleson (elasLlc flbers)
+ 8ouLlne flxaLlve
+ reserves mlLochondrla
- musL be washed overnlghL Lo remove excess chromaLe
- requlres lodlne LreaLmenL Lo remove mercury before rouLlne
sLalnlng
- musL be prepared fresh
- molecular analysls
- no meLal lnsLrumenLs
- lPC
2enker's formo| - 8M
- Spleen
- All blood conLalnlng
organs
+ rouLlne flxaLlve
+ preserves mlLochondrla
+ preserves 88Cs
- musL be washed overnlghL Lo remove chromaLe
- requlres lodlne LreaLmenL Lo remove mercury before rouLlne
sLalnlng
- molecular analysls
- lPC
8ou|n's - 1esLlcular 8x's + Masson Lrlchrome (collagen & muscle) + rouLlne flxaLlve
- lyses 88Cs
- removes ferrlc lron
- dlssolves some proLelns
- molecular analysls
- lPC
- carbohydraLes
Lthy| a|coho| - All + Congo red (amylold)
+ von kossa's (Ca2+)
+ WelgerL's sLaln for flbrln
+ Mallory's sLaln for lron
+ Comorl's meLhenamlne sllver
- Zlehl-neelsen (Al8)
+ Lnzyme hlsLochemlsLry
+ molecular analysls
+ lmpresslon smears
+ 8lood smears
+ reserves glycogen
+ reserves crysLals: urlc acld, sodlum uraLe
- causes excesslve hardenlng
- rouLlne flxaLlve
- ulssolves llplds
Acetone 0-4C - All - Zlehl-neelsen (Al8) + enzyme hlsLochemlsLry
- rouLlne flxaLlve
- for besL resulLs, musL be refrlgeraLed
- dlssolves llplds
Carnoy's - All + meLhyl green pyronln (unA & 8nA)
+ Congo red (amylold)
+ Clemsa (masL cells)
- Zlehl-neelsen (Al8)
+ cyLologlc flxaLlve
+ rapld peneLraLlon
+ nuclear deLall
+ llxes 8nA
+ reserves glycogen
- dlssolves cyLoplasmlc elemenLs
- Pemolyzes ls 88Cs
CCMMCN IIkA1IVLS