Beruflich Dokumente
Kultur Dokumente
Introduction
Mechanism of IR absorption
There is no change in dipole moment during the symmetric stretch vibration and the 1340
cm-1 band is not observed in the infrared absorption spectrum (the symmetric stretch is
called infrared inactive). There is a change in dipole moment during the asymmetric
stretch and the 2350 cm-1 band does absorb infrared radiation (the asymmetric stretch in
infrared active). A related vibrational spectroscopic method is Raman spectroscopy,
which has a different mechanism and therefore provides complementary information to
infrared absorption.
Infrared Absorption Spectrometers
Introduction
This document describes dispersive and Fourier-transform spectrometers that are used in
infrared absorption spectroscopy.
Common light sources are tungsten lamps, Nernst glowers, or glowbars. Dispersive IR
spectrometers use a grating monochromator to select wavelengths and are commonly
used when a single wavelength is desired to monitor the kinetics of a reaction or as a GC
or LC detector.
IR absorption spectroscopy uses mid-infrared light (2.5 - 50 µm, 4000 - 200 cm-1) to
detect specific types of chemical bonds in a sample for identification of organic and
organometallic molecules.
NIR spectroscopy is the measurement of the wavelength and intensity of the absorption
of near-infrared light by a sample. Near-infrared light spans the 800 nm - 2.5 µm (12,500
- 4000 cm-1) range and is energetic enough to excite overtones and combinations of
molecular vibrations to higher energy levels. NIR spectroscopy is typically used for
quantitative measurement of organic functional groups, especially O-H, N-H, and C=O.
Detection limits are typically 0.1% and applications include pharmaceutical, agricultural,
polymer, and clinical analysis.
Instrumentation
The components and design of NIR instrumentation are similar to uv-vis absorption
spectrometers. The light source is usually a tungsten lamp and the detector is usually a
PbS solid-state detector. Sample holders can be glass or quartz and typical solvents are
CCl4 and CS2. The convenient instrumentation of NIR spectroscopy compared to IR
spectroscopy makes it much more suitable for on-line monitoring and process control.