BPC-157 Achilles Tendons of Rats

Posted on April 19, 2014 by Maxim Peptide

BPC-157 is becoming significantly more popular as a research peptide as scientists continue to determine the
oerall benefits of using this substance to boost the ability of an animal to heal damaged tissue! "uch of the
research# at this point# reoles around determining the full use of this product as $ell as the potential implications
of administering BPC-157 to different animals# determining if it $ill# in fact# behae in the same $ay% regardless of
the type of tissue that it interacts $ith!
BPC-157 is a pentadecapeptide that has 15 amino acids! This is a partial se&uence of a natural body protection
compound $hich $as discoered $ithin gastric 'uices and determined that it could be isolated for further uses
throughout an animal(s body!
)n e*periments $ith lie animals it has been found that applying BPC-157 can accelerate the healing process in a
ariety of different types of $ounds! This $as most commonly tested in the Achilles tendons of rats!
BPC-157 in the Achilles Tendons of Rats
The study $as performed to determine the potential mechanisms housed $ithin BPC-157 to enhance in'ured
tendons in healing!
+uring the e*periment# the outgro$th of the fibroblasts $ithin the e*plants of the tendons $as cultured $ith or
$ithout the application of BPC-157 to e*amine the process!
Results of this study indicated that cell proliferation of the fibroblasts that $ere deried from the Achilles tendon
in the rat $as not effected directly by the presence of BPC-157! ,o$eer# the cells that $ere treated $ith BP--
157 sa$ a significant increase in their surial rate# compared to those that $ere e*periencing stress from the
,./01./0 stress!
The presence of BPC-157 mar2ed a noticeable increase in the itro migration of the tendon fibroblasts! These
results $ere application-dependent $hich $as reealed based on the spreading of these fibroblasts in samples#
housed in culture dishes!
The 3-actin formations that $ere detected in the 3)TC-phalloidin staining $ere introduced along $ith the
fibroblasts that $ere treated $ith BPC-157! The e*pression of proteins that actiated the pa*illin and 3A4 in
these samples $as tested by 5estern blot analysis!
The analysis of these samples $as found to sho$ application-dependent increases in BPC-157! 6imultaneously#
the total amount of protein in these samples $as not altered by these effects! This allo$ed researchers to conclude
that BPC-157 is capable of promoting e*-io outgro$th from tendon fibroblasts $hich include tendon e*plants!
This peptide can also aid in the surial of cells e*periencing stress and the in itro migration of fibroblasts from
tendons! This is li2ely to be mediated by actiating the path$ays of the 3A4-pa*illin in an animal(s body!
Anti-inflammatory 7ffects on 7*perimental Periodonitis
BPC-157 $as tested for its anti-8inflammatory effects# along $ith its ability to heal $ounds in organs and targeted
tissues!
The method for testing this ability reoled around inestigating the effects of this peptide on bone resorption
and inflammation in rats $ith e*perimental periodontitis!
3irst the acute effects of BPC-157 $ere tested by applying the chemical using laser +oppler flo$metry on the
gingial blood flo$! Periodontitis $as then produced using a sil2 ligature that $as placed on the left lo$er molar!
Rats that $ere proided applications of BPC-157 $ere assessed using the 7ans blue plasma techni&ue as
$ell as histology $hile Aleolar bone loss $as monitored using microCT!
1erall# BPC-157 $as sho$n to reduce plasma e*caation significantly and improed aleolar bone destruction!
This did not impact the blood circulation around healthy gingia! )t also suggests that this peptide could potentially
be used to manage periodontal disease!
Preventing Gastric Lesions
9astric damage from ethanol administration in rats is unli2e the chronic administration of alcohol in drin2ing $ater
$hich leads to portal hypertension and lier lesions!
A ariety of chemicals are 2no$n to assist in reducing these effects! 6pecifically# applying BPC-157 has been
found to help reduce gastrointestinal models!
This chemical $as applied alongside propranol and saline! These $ere administered once a day starting 1:
days before the alcohol $as consumed or since the drin2ing began! 6ome also began t$o months after the
alcohol consumption began for the sa2e of comparison!
5hen applied the pertinent lesions on the stomach mucosa $as antagoni;ed but the appearance of lesions
lessened $hen the chemical $as administered daily# throughout the periods $here alcohol $as consumed! These
results indicate that BPC-157 administered in combination $ith propranol and ranitdine could help reerse the
effects of gastric lesions on stomach mucosa that occur from chronic drin2ing and could hae potential in future
therapy!
The isolation process for natural BPC-157 is some$hat delicate! 9ien the care that must be ta2en in preparing
samples for study# it is ital that indiiduals that plan to use BPC-157 in a research setting ma2e the effort to
purchase this peptide from a &ualified buyer! )t is also important to do careful research on the stability of this
peptide# to ensure that all trials can be replicated accurately and treated in a manner that $ill allo$ for accurate
research!
Resource Bo*<
http<==$$$!ncbi!nlm!nih!go=pubmed=/1:>:?7/
http<==$$$!ma*impeptide!com=bpc-157-5mg=