CE-IVD-Marked

Bethesda 2006 International

Consensus-Aligned
WHO 2008-Revised
Classifcation-Compatible
5-Color, 7-Combination,
23-Marker Panel
Pre-mixed, Optimized,
Comprehensive Cocktails
Provide Standardization,
Consistency and Simplicity
Blood Banking
Capillary Electrophoresis
Cell Analysis
Centrifugation
Genomics
Lab Automation
Lab Tools
Particle Characterization
Solastra

Lineage Panel
Experience
simplicity and
consistency
in multiparametric fow cytometry
PRODUCT OVERVI EW
Flow cytometric analyses are an integral part of the stan-
dard of care in hematopathology. The Solastra 5-Color
Reagent Panel is a comprehensive CE-marked panel
of conjugated-antibody cocktails for the characterization
of hematolymphoid neoplasia by fow cytometry. These
products are intended for identifcation and enumeration
of relevant leukocyte surface molecules and are useful
as an aid in differential diagnosis of patients with certain
abnormal hematology results and/or presence of blasts in
the blood stream, bone marrow and/or lymphoid tissues.
The Solastra 5-Color Reagents are composed of antibodies
directed to B, T, and Myelomonocytic lineage antigens and
refect the minimum number of markers recommended by
the 2006 Bethesda International Consensus conference
published conclusions (1, 2).
MARKERS
The following markers are included in the kits,
respectively:
B Lineage Kit: CD45, CD5, CD10, CD19, CD20,
CD38, Kappa and Lambda
T Lineage Kit: CD45, CD2, CD3, CD4, CD5, CD7,
CD8 and CD56
Myelomonocytic Lineage Kit: CD45, CD7, CD11b,
CD13, CD14, CD15, CD16, CD33, CD34, CD56,
CD117 and HLA-DR
MARKER CLONE REACTI VI TY
CD2 39C1.5
IgG2a (rat)
T cells and most of the NK cells
CD3 UCHT1
IgG1 (mouse)
Mature T cell (cytoplasmic expres-
sion in immature T cells)
CD4 SFCI12T4D11
IgG1 (mouse)
Helper / inducer T cells, monocytes,
immature myeloid cells
CD5 BL1a
IgG2a (mouse)
Thymocytes, mature T cells, subpo-
pulation of B cells
CD7 8H8.1
IgG2a (mouse)
T cells, NK cells, subpopulation of
immature myeloid cells
CD8 SFCI21Thy2D3
IgG1 (mouse)
Cytotoxic / suppressor T cells, sub-
population of NK cells
CD10 ALB1
IgG1 (mouse)
Common acute leukemia antigen
(CALLA), lymphatic precursor cells,
neutrophils, subpopulation of mature
B cells
CD11b Bear1
IgG1 (mouse)
Monocytes, macrophages, neu-
trophils, NK cells
CD13 366 (MY7)
IgG1 (mouse)
Myeloid cells
CD14 RMO52
IgG2a (mouse)
Monocytes, weak expression on
neutrophils
CD15 80H5
IgM (mouse)
Neutrophils, weak expression on
monocytes
CD16 3G8
IgG1 (mouse)
NK cells, neutrophils, subpopulation of
monocytes
CD19 J3-119
IgG1 (mouse)
Precursor and mature B cells
MARKER CLONE REACTI VI TY
CD20 B9E9
IgG2a (mouse)
B cells and a subpopulation of B
precursor cells
CD33 D3HL60.251
IgG1 (mouse)
Monocytes, myeloid precursor cells,
weak on neutrophils
CD34 581
IgG1 (mouse)
Myeloid and lymphoid precursor
cells
CD38 LS198-4-3
IgG1 (mouse)
Activated lymphocytes, subpopula-
tion of B cells, plasma cells
CD45 J.33
IgG1 (mouse)
All leukocytes
CD56 N901
IgG1 (mouse)
NK cells, subpopulation of T cells
CD117 104D2D1
IgG1 (mouse)
Myeloid precursor cells
HLA-DR Immu-357
IgG1 (mouse)
B cells, activated T cells, monocytes,
precursor cells
Kappa N/A: Polyclonal Kappa light chain
Lambda N/A: Polyclonal Lambda light chain
PRODUCTS
The three Beckman Coulter Solastra products (T-, B- and
Myelomonocytic-Lineage Kits) consist of 7 pre-mixed, CE-
IVD, 5-color cocktails. A total of 23 markers are included
as directly conjugated antibodies. As recommended in
the Bethesda International Consensus, CD45 is present
in each combination for gating purposes. Dual parameter
histograms gated on the selected CD45 vs. Side Scatter
populations are then used to determine positively stained
cells for each of the surface antigens recognized by the
antibodies within the kits.
SOL ASTR A 5- COLOR KI TS AND COMBI NATI ONS
PN PANEL TUBE FITC PE ECD PC5.5 PC7
A66286
Solastra B
Lineage Kit
BL1 Kappa Lambda CD19 CD5 CD45
BL2 CD20 CD10 CD19 CD38 CD45
A66287
Solastra T
Lineage Kit
TL1 CD2 CD56 CD7 CD5 CD45
TL2 CD8 CD4 - CD3 CD45
A66288
Solastra
Myelomo-
nocytic
Lineage Kit
ML1 CD15 CD11b CD16 CD14 CD45
ML2 HLA-DR CD56 CD34 CD117 CD45
ML3 CD7 CD13 CD34 CD33 CD45
STOR AGE CONDI TI ONS AND STABI LI T Y:
Closed vial stability: 9 months maximum after date of manu-
facturing when stored in standard laboratory refrigerators,
i.e. at 2 8C. Do not freeze. Opened vials are stable for
90 days, provided that care is taken to minimize exposure
to light and return to 2 8C immediately after use.
originalet.indd 2 10-06-18 15.23.14
PRODUCT OVERVI EW
Flow cytometric analyses are an integral part of the stan-
dard of care in hematopathology. The Solastra 5-Color
Reagent Panel is a comprehensive CE-marked panel
of conjugated-antibody cocktails for the characterization
of hematolymphoid neoplasia by fow cytometry. These
products are intended for identifcation and enumeration
of relevant leukocyte surface molecules and are useful
as an aid in differential diagnosis of patients with certain
abnormal hematology results and/or presence of blasts in
the blood stream, bone marrow and/or lymphoid tissues.
The Solastra 5-Color Reagents are composed of antibodies
directed to B, T, and Myelomonocytic lineage antigens and
refect the minimum number of markers recommended by
the 2006 Bethesda International Consensus conference
published conclusions (1, 2).
MARKERS
The following markers are included in the kits,
respectively:
B Lineage Kit: CD45, CD5, CD10, CD19, CD20,
CD38, Kappa and Lambda
T Lineage Kit: CD45, CD2, CD3, CD4, CD5, CD7,
CD8 and CD56
Myelomonocytic Lineage Kit: CD45, CD7, CD11b,
CD13, CD14, CD15, CD16, CD33, CD34, CD56,
CD117 and HLA-DR
MARKER CLONE REACTI VI TY
CD2 39C1.5
IgG2a (rat)
T cells and most of the NK cells
CD3 UCHT1
IgG1 (mouse)
Mature T cell (cytoplasmic expres-
sion in immature T cells)
CD4 SFCI12T4D11
IgG1 (mouse)
Helper / inducer T cells, monocytes,
immature myeloid cells
CD5 BL1a
IgG2a (mouse)
Thymocytes, mature T cells, subpo-
pulation of B cells
CD7 8H8.1
IgG2a (mouse)
T cells, NK cells, subpopulation of
immature myeloid cells
CD8 SFCI21Thy2D3
IgG1 (mouse)
Cytotoxic / suppressor T cells, sub-
population of NK cells
CD10 ALB1
IgG1 (mouse)
Common acute leukemia antigen
(CALLA), lymphatic precursor cells,
neutrophils, subpopulation of mature
B cells
CD11b Bear1
IgG1 (mouse)
Monocytes, macrophages, neu-
trophils, NK cells
CD13 366 (MY7)
IgG1 (mouse)
Myeloid cells
CD14 RMO52
IgG2a (mouse)
Monocytes, weak expression on
neutrophils
CD15 80H5
IgM (mouse)
Neutrophils, weak expression on
monocytes
CD16 3G8
IgG1 (mouse)
NK cells, neutrophils, subpopulation of
monocytes
CD19 J3-119
IgG1 (mouse)
Precursor and mature B cells
MARKER CLONE REACTI VI TY
CD20 B9E9
IgG2a (mouse)
B cells and a subpopulation of B
precursor cells
CD33 D3HL60.251
IgG1 (mouse)
Monocytes, myeloid precursor cells,
weak on neutrophils
CD34 581
IgG1 (mouse)
Myeloid and lymphoid precursor
cells
CD38 LS198-4-3
IgG1 (mouse)
Activated lymphocytes, subpopula-
tion of B cells, plasma cells
CD45 J.33
IgG1 (mouse)
All leukocytes
CD56 N901
IgG1 (mouse)
NK cells, subpopulation of T cells
CD117 104D2D1
IgG1 (mouse)
Myeloid precursor cells
HLA-DR Immu-357
IgG1 (mouse)
B cells, activated T cells, monocytes,
precursor cells
Kappa N/A: Polyclonal Kappa light chain
Lambda N/A: Polyclonal Lambda light chain
PRODUCTS
The three Beckman Coulter Solastra products (T-, B- and
Myelomonocytic-Lineage Kits) consist of 7 pre-mixed, CE-
IVD, 5-color cocktails. A total of 23 markers are included
as directly conjugated antibodies. As recommended in
the Bethesda International Consensus, CD45 is present
in each combination for gating purposes. Dual parameter
histograms gated on the selected CD45 vs. Side Scatter
populations are then used to determine positively stained
cells for each of the surface antigens recognized by the
antibodies within the kits.
SOL ASTR A 5- COLOR KI TS AND COMBI NATI ONS
PN PANEL TUBE FITC PE ECD PC5.5 PC7
A66286
Solastra B
Lineage Kit
BL1 Kappa Lambda CD19 CD5 CD45
BL2 CD20 CD10 CD19 CD38 CD45
A66287
Solastra T
Lineage Kit
TL1 CD2 CD56 CD7 CD5 CD45
TL2 CD8 CD4 - CD3 CD45
A66288
Solastra
Myelomo-
nocytic
Lineage Kit
ML1 CD15 CD11b CD16 CD14 CD45
ML2 HLA-DR CD56 CD34 CD117 CD45
ML3 CD7 CD13 CD34 CD33 CD45
STOR AGE CONDI TI ONS AND STABI LI T Y:
Closed vial stability: 9 months maximum after date of manu-
facturing when stored in standard laboratory refrigerators,
i.e. at 2 8C. Do not freeze. Opened vials are stable for
90 days, provided that care is taken to minimize exposure
to light and return to 2 8C immediately after use.
originalet.indd 2 10-06-18 15.23.14
PRODUCT OVERVI EW
Flow cytometric analyses are an integral part of the stan-
dard of care in hematopathology. The Solastra 5-Color
Reagent Panel is a comprehensive CE-marked panel
of conjugated-antibody cocktails for the characterization
of hematolymphoid neoplasia by fow cytometry. These
products are intended for identifcation and enumeration
of relevant leukocyte surface molecules and are useful
as an aid in differential diagnosis of patients with certain
abnormal hematology results and/or presence of blasts in
the blood stream, bone marrow and/or lymphoid tissues.
The Solastra 5-Color Reagents are composed of antibodies
directed to B, T, and Myelomonocytic lineage antigens and
refect the minimum number of markers recommended by
the 2006 Bethesda International Consensus conference
published conclusions (1, 2).
MARKERS
The following markers are included in the kits,
respectively:
B Lineage Kit: CD45, CD5, CD10, CD19, CD20,
CD38, Kappa and Lambda
T Lineage Kit: CD45, CD2, CD3, CD4, CD5, CD7,
CD8 and CD56
Myelomonocytic Lineage Kit: CD45, CD7, CD11b,
CD13, CD14, CD15, CD16, CD33, CD34, CD56,
CD117 and HLA-DR
MARKER CLONE REACTI VI TY
CD2 39C1.5
IgG2a (rat)
T cells and most of the NK cells
CD3 UCHT1
IgG1 (mouse)
Mature T cell (cytoplasmic expres-
sion in immature T cells)
CD4 SFCI12T4D11
IgG1 (mouse)
Helper / inducer T cells, monocytes,
immature myeloid cells
CD5 BL1a
IgG2a (mouse)
Thymocytes, mature T cells, subpo-
pulation of B cells
CD7 8H8.1
IgG2a (mouse)
T cells, NK cells, subpopulation of
immature myeloid cells
CD8 SFCI21Thy2D3
IgG1 (mouse)
Cytotoxic / suppressor T cells, sub-
population of NK cells
CD10 ALB1
IgG1 (mouse)
Common acute leukemia antigen
(CALLA), lymphatic precursor cells,
neutrophils, subpopulation of mature
B cells
CD11b Bear1
IgG1 (mouse)
Monocytes, macrophages, neu-
trophils, NK cells
CD13 366 (MY7)
IgG1 (mouse)
Myeloid cells
CD14 RMO52
IgG2a (mouse)
Monocytes, weak expression on
neutrophils
CD15 80H5
IgM (mouse)
Neutrophils, weak expression on
monocytes
CD16 3G8
IgG1 (mouse)
NK cells, neutrophils, subpopulation of
monocytes
CD19 J3-119
IgG1 (mouse)
Precursor and mature B cells
MARKER CLONE REACTI VI TY
CD20 B9E9
IgG2a (mouse)
B cells and a subpopulation of B
precursor cells
CD33 D3HL60.251
IgG1 (mouse)
Monocytes, myeloid precursor cells,
weak on neutrophils
CD34 581
IgG1 (mouse)
Myeloid and lymphoid precursor
cells
CD38 LS198-4-3
IgG1 (mouse)
Activated lymphocytes, subpopula-
tion of B cells, plasma cells
CD45 J.33
IgG1 (mouse)
All leukocytes
CD56 N901
IgG1 (mouse)
NK cells, subpopulation of T cells
CD117 104D2D1
IgG1 (mouse)
Myeloid precursor cells
HLA-DR Immu-357
IgG1 (mouse)
B cells, activated T cells, monocytes,
precursor cells
Kappa N/A: Polyclonal Kappa light chain
Lambda N/A: Polyclonal Lambda light chain
PRODUCTS
The three Beckman Coulter Solastra products (T-, B- and
Myelomonocytic-Lineage Kits) consist of 7 pre-mixed, CE-
IVD, 5-color cocktails. A total of 23 markers are included
as directly conjugated antibodies. As recommended in
the Bethesda International Consensus, CD45 is present
in each combination for gating purposes. Dual parameter
histograms gated on the selected CD45 vs. Side Scatter
populations are then used to determine positively stained
cells for each of the surface antigens recognized by the
antibodies within the kits.
SOL ASTR A 5- COLOR KI TS AND COMBI NATI ONS
PN PANEL TUBE FITC PE ECD PC5.5 PC7
A66286
Solastra B
Lineage Kit
BL1 Kappa Lambda CD19 CD5 CD45
BL2 CD20 CD10 CD19 CD38 CD45
A66287
Solastra T
Lineage Kit
TL1 CD2 CD56 CD7 CD5 CD45
TL2 CD8 CD4 - CD3 CD45
A66288
Solastra
Myelomo-
nocytic
Lineage Kit
ML1 CD15 CD11b CD16 CD14 CD45
ML2 HLA-DR CD56 CD34 CD117 CD45
ML3 CD7 CD13 CD34 CD33 CD45
STOR AGE CONDI TI ONS AND STABI LI T Y:
Closed vial stability: 9 months maximum after date of manu-
facturing when stored in standard laboratory refrigerators,
i.e. at 2 8C. Do not freeze. Opened vials are stable for
90 days, provided that care is taken to minimize exposure
to light and return to 2 8C immediately after use.
originalet.indd 2 10-06-18 15.23.14
MARKER CLONE REACTI VI TY
CD20 B9E9
IgG2a (mouse)
B cells and a subpopulation of B
precursor cells
CD33 D3HL60.251
IgG1 (mouse)
Monocytes, myeloid precursor cells,
weak on neutrophils
CD34 581
IgG1 (mouse)
Myeloid and lymphoid precursor
cells
CD38 LS198-4-3
IgG1 (mouse)
Activated lymphocytes, subpopula-
tion of B cells, plasma cells
CD45 J.33
IgG1 (mouse)
All leukocytes
CD56 N901
IgG1 (mouse)
NK cells, subpopulation of T cells
CD117 104D2D1
IgG1 (mouse)
Myeloid precursor cells
HLA-DR Immu-357
IgG1 (mouse)
B cells, activated T cells, monocytes,
precursor cells
Kappa N/A: Polyclonal Kappa light chain
Lambda N/A: Polyclonal Lambda light chain
PRODUCTS
The three Beckman Coulter Solastra products (T-, B- and
Myelomonocytic-Lineage Kits) consist of 7 pre-mixed, CE-
IVD, 5-color cocktails. A total of 23 markers are included
as directly conjugated antibodies. As recommended in
the Bethesda International Consensus, CD45 is present
in each combination for gating purposes. Dual parameter
histograms gated on the selected CD45 vs. Side Scatter
populations are then used to determine positively stained
cells for each of the surface antigens recognized by the
antibodies within the kits.
SOL ASTR A 5- COLOR KI TS AND COMBI NATI ONS
PN PANEL TUBE FITC PE ECD PC5.5 PC7
A66286
Solastra B
Lineage Kit
BL1 Kappa Lambda CD19 CD5 CD45
BL2 CD20 CD10 CD19 CD38 CD45
A66287
Solastra T
Lineage Kit
TL1 CD2 CD56 CD7 CD5 CD45
TL2 CD8 CD4 - CD3 CD45
A66288
Solastra
Myelomo-
nocytic
Lineage Kit
ML1 CD15 CD11b CD16 CD14 CD45
ML2 HLA-DR CD56 CD34 CD117 CD45
ML3 CD7 CD13 CD34 CD33 CD45
STOR AGE CONDI TI ONS AND STABI LI T Y:
Closed vial stability: 9 months maximum after date of manu-
facturing when stored in standard laboratory refrigerators,
i.e. at 2 8C. Do not freeze. Opened vials are stable for
90 days, provided that care is taken to minimize exposure
to light and return to 2 8C immediately after use.
RE AGENTS REQUI RED BUT NOT SUPPLI ED
VersaLyse Lysing Solution, PN A09777
IOTest 3 Fixative Solution, PN A07800
Flow-Check Pro Fluorospheres, PN A63493
Flow-Set Pro Fluorospheres, PN A63492
Solastra QuickCOMP 5 Kit, PN A83571

or
QuickCOMP 4 Kit (except for CD45-PC5),
PN 177017, with CD45-PC5.5, PN A62835, and
CD45-PC7, PN IM3548
Phosphate Buffered Saline (PBS), PN 6603369
Heat-Inactivated Fetal Calf Serum (HIFCS)
Heat-Inactivated Mouse Serum (HIMS) (facultative)
FLOW CY TOMETERS AND FLUOROCHROMES:
Solastra is well adapted to Beckman Coulter Cytomics FC
500 Flow Cytometers, capable of simultaneous 5-color
analysis from a single 488 nm laser line. Fluorochromes
are usable without flter change on the FC 500 confgured
with the standard IVD application flter set for 5-color single
laser analysis. If necessary, optical bench can be adapted
to the PC5.5 fuorochrome emission spectrum by using
the following devices: Single Laser Filter Kit for FC 500
fow cytometer, PN 179044 or Single Laser Filter Block
Assembly for FC 500 fow cytometer, PN 179045.
FLUOROCHROME PRIMARY
EXCITATION (nm)
MAXIMUM
EMISSION (nm)
FITC 468-509 504-541
PE 486-580 568-590
ECD 486-580 610-635
PC5.5 486-580 680-710
PC7 486-580 750-790
I NSTRUMENT SET TI NGS:
Users should refer to the instruments manuals for speci-
fc instructions for setting PMT voltages and fuorescence
compensation prior to analysis; autostandardization and
compensation are to be done with CXP software, using Flow-
Check Pro Fluorospheres, Flow-Set Pro Fluorospheres, and
fuorochrome matched CD45 conjugates.
SPECI MEN COLLECTI ON:
Each fow cytometric analysis requires 100 L of whole
blood, bone marrow or single lymphoid cell suspension
(specimens should be with white blood cell counts in
the range of 2-20 x 10
3
cells/L).
Whole Blood and bone marrow may be collected using
EDTA, Heparin or ACD anticoagulants as appropriate
for the specimen.
EDTA collected specimens stained with Solastra Rea-
gents may be prepared within 24 hours. Analysis of
samples must be performed on the same day as sample
staining. Specimens collected with Heparin or ACD may
be prepared within 48 hours of collection. Analysis of
samples must be performed on the same day as sample
staining.
SPECI MEN PREPAR ATI ON:
A pre-wash procedure is required to optimize the staining
of Kappa- and Lambda-Light Chains on cell surface. It is
generalized to the whole panel with the aim to standardize
the preparation and staining procedures. Freedom is given
to the user to choose a bulk wash procedure where the
totality of the specimen is washed at once, or a classical
single tube wash procedure, where specimen aliquots
are washed separately.
originalet.indd 3 10-06-18 15.23.20
MARKER CLONE REACTI VI TY
CD20 B9E9
IgG2a (mouse)
B cells and a subpopulation of B
precursor cells
CD33 D3HL60.251
IgG1 (mouse)
Monocytes, myeloid precursor cells,
weak on neutrophils
CD34 581
IgG1 (mouse)
Myeloid and lymphoid precursor
cells
CD38 LS198-4-3
IgG1 (mouse)
Activated lymphocytes, subpopula-
tion of B cells, plasma cells
CD45 J.33
IgG1 (mouse)
All leukocytes
CD56 N901
IgG1 (mouse)
NK cells, subpopulation of T cells
CD117 104D2D1
IgG1 (mouse)
Myeloid precursor cells
HLA-DR Immu-357
IgG1 (mouse)
B cells, activated T cells, monocytes,
precursor cells
Kappa N/A: Polyclonal Kappa light chain
Lambda N/A: Polyclonal Lambda light chain
PRODUCTS
The three Beckman Coulter Solastra products (T-, B- and
Myelomonocytic-Lineage Kits) consist of 7 pre-mixed, CE-
IVD, 5-color cocktails. A total of 23 markers are included
as directly conjugated antibodies. As recommended in
the Bethesda International Consensus, CD45 is present
in each combination for gating purposes. Dual parameter
histograms gated on the selected CD45 vs. Side Scatter
populations are then used to determine positively stained
cells for each of the surface antigens recognized by the
antibodies within the kits.
SOL ASTR A 5- COLOR KI TS AND COMBI NATI ONS
PN PANEL TUBE FITC PE ECD PC5.5 PC7
A66286
Solastra B
Lineage Kit
BL1 Kappa Lambda CD19 CD5 CD45
BL2 CD20 CD10 CD19 CD38 CD45
A66287
Solastra T
Lineage Kit
TL1 CD2 CD56 CD7 CD5 CD45
TL2 CD8 CD4 - CD3 CD45
A66288
Solastra
Myelomo-
nocytic
Lineage Kit
ML1 CD15 CD11b CD16 CD14 CD45
ML2 HLA-DR CD56 CD34 CD117 CD45
ML3 CD7 CD13 CD34 CD33 CD45
STOR AGE CONDI TI ONS AND STABI LI T Y:
Closed vial stability: 9 months maximum after date of manu-
facturing when stored in standard laboratory refrigerators,
i.e. at 2 8C. Do not freeze. Opened vials are stable for
90 days, provided that care is taken to minimize exposure
to light and return to 2 8C immediately after use.
RE AGENTS REQUI RED BUT NOT SUPPLI ED
VersaLyse Lysing Solution, PN A09777
IOTest 3 Fixative Solution, PN A07800
Flow-Check Pro Fluorospheres, PN A63493
Flow-Set Pro Fluorospheres, PN A63492
Solastra QuickCOMP 5 Kit, PN A83571

or
QuickCOMP 4 Kit (except for CD45-PC5),
PN 177017, with CD45-PC5.5, PN A62835, and
CD45-PC7, PN IM3548
Phosphate Buffered Saline (PBS), PN 6603369
Heat-Inactivated Fetal Calf Serum (HIFCS)
Heat-Inactivated Mouse Serum (HIMS) (facultative)
FLOW CY TOMETERS AND FLUOROCHROMES:
Solastra is well adapted to Beckman Coulter Cytomics FC
500 Flow Cytometers, capable of simultaneous 5-color
analysis from a single 488 nm laser line. Fluorochromes
are usable without flter change on the FC 500 confgured
with the standard IVD application flter set for 5-color single
laser analysis. If necessary, optical bench can be adapted
to the PC5.5 fuorochrome emission spectrum by using
the following devices: Single Laser Filter Kit for FC 500
fow cytometer, PN 179044 or Single Laser Filter Block
Assembly for FC 500 fow cytometer, PN 179045.
FLUOROCHROME PRIMARY
EXCITATION (nm)
MAXIMUM
EMISSION (nm)
FITC 468-509 504-541
PE 486-580 568-590
ECD 486-580 610-635
PC5.5 486-580 680-710
PC7 486-580 750-790
I NSTRUMENT SET TI NGS:
Users should refer to the instruments manuals for speci-
fc instructions for setting PMT voltages and fuorescence
compensation prior to analysis; autostandardization and
compensation are to be done with CXP software, using Flow-
Check Pro Fluorospheres, Flow-Set Pro Fluorospheres, and
fuorochrome matched CD45 conjugates.
SPECI MEN COLLECTI ON:
Each fow cytometric analysis requires 100 L of whole
blood, bone marrow or single lymphoid cell suspension
(specimens should be with white blood cell counts in
the range of 2-20 x 10
3
cells/L).
Whole Blood and bone marrow may be collected using
EDTA, Heparin or ACD anticoagulants as appropriate
for the specimen.
EDTA collected specimens stained with Solastra Rea-
gents may be prepared within 24 hours. Analysis of
samples must be performed on the same day as sample
staining. Specimens collected with Heparin or ACD may
be prepared within 48 hours of collection. Analysis of
samples must be performed on the same day as sample
staining.
SPECI MEN PREPAR ATI ON:
A pre-wash procedure is required to optimize the staining
of Kappa- and Lambda-Light Chains on cell surface. It is
generalized to the whole panel with the aim to standardize
the preparation and staining procedures. Freedom is given
to the user to choose a bulk wash procedure where the
totality of the specimen is washed at once, or a classical
single tube wash procedure, where specimen aliquots
are washed separately.
originalet.indd 3 10-06-18 15.23.20
MARKER CLONE REACTI VI TY
CD20 B9E9
IgG2a (mouse)
B cells and a subpopulation of B
precursor cells
CD33 D3HL60.251
IgG1 (mouse)
Monocytes, myeloid precursor cells,
weak on neutrophils
CD34 581
IgG1 (mouse)
Myeloid and lymphoid precursor
cells
CD38 LS198-4-3
IgG1 (mouse)
Activated lymphocytes, subpopula-
tion of B cells, plasma cells
CD45 J.33
IgG1 (mouse)
All leukocytes
CD56 N901
IgG1 (mouse)
NK cells, subpopulation of T cells
CD117 104D2D1
IgG1 (mouse)
Myeloid precursor cells
HLA-DR Immu-357
IgG1 (mouse)
B cells, activated T cells, monocytes,
precursor cells
Kappa N/A: Polyclonal Kappa light chain
Lambda N/A: Polyclonal Lambda light chain
PRODUCTS
The three Beckman Coulter Solastra products (T-, B- and
Myelomonocytic-Lineage Kits) consist of 7 pre-mixed, CE-
IVD, 5-color cocktails. A total of 23 markers are included
as directly conjugated antibodies. As recommended in
the Bethesda International Consensus, CD45 is present
in each combination for gating purposes. Dual parameter
histograms gated on the selected CD45 vs. Side Scatter
populations are then used to determine positively stained
cells for each of the surface antigens recognized by the
antibodies within the kits.
SOL ASTR A 5- COLOR KI TS AND COMBI NATI ONS
PN PANEL TUBE FITC PE ECD PC5.5 PC7
A66286
Solastra B
Lineage Kit
BL1 Kappa Lambda CD19 CD5 CD45
BL2 CD20 CD10 CD19 CD38 CD45
A66287
Solastra T
Lineage Kit
TL1 CD2 CD56 CD7 CD5 CD45
TL2 CD8 CD4 - CD3 CD45
A66288
Solastra
Myelomo-
nocytic
Lineage Kit
ML1 CD15 CD11b CD16 CD14 CD45
ML2 HLA-DR CD56 CD34 CD117 CD45
ML3 CD7 CD13 CD34 CD33 CD45
STOR AGE CONDI TI ONS AND STABI LI T Y:
Closed vial stability: 9 months maximum after date of manu-
facturing when stored in standard laboratory refrigerators,
i.e. at 2 8C. Do not freeze. Opened vials are stable for
90 days, provided that care is taken to minimize exposure
to light and return to 2 8C immediately after use.
RE AGENTS REQUI RED BUT NOT SUPPLI ED
VersaLyse Lysing Solution, PN A09777
IOTest 3 Fixative Solution, PN A07800
Flow-Check Pro Fluorospheres, PN A63493
Flow-Set Pro Fluorospheres, PN A63492
Solastra QuickCOMP 5 Kit, PN A83571

or
QuickCOMP 4 Kit (except for CD45-PC5),
PN 177017, with CD45-PC5.5, PN A62835, and
CD45-PC7, PN IM3548
Phosphate Buffered Saline (PBS), PN 6603369
Heat-Inactivated Fetal Calf Serum (HIFCS)
Heat-Inactivated Mouse Serum (HIMS) (facultative)
FLOW CY TOMETERS AND FLUOROCHROMES:
Solastra is well adapted to Beckman Coulter Cytomics FC
500 Flow Cytometers, capable of simultaneous 5-color
analysis from a single 488 nm laser line. Fluorochromes
are usable without flter change on the FC 500 confgured
with the standard IVD application flter set for 5-color single
laser analysis. If necessary, optical bench can be adapted
to the PC5.5 fuorochrome emission spectrum by using
the following devices: Single Laser Filter Kit for FC 500
fow cytometer, PN 179044 or Single Laser Filter Block
Assembly for FC 500 fow cytometer, PN 179045.
FLUOROCHROME PRIMARY
EXCITATION (nm)
MAXIMUM
EMISSION (nm)
FITC 468-509 504-541
PE 486-580 568-590
ECD 486-580 610-635
PC5.5 486-580 680-710
PC7 486-580 750-790
I NSTRUMENT SET TI NGS:
Users should refer to the instruments manuals for speci-
fc instructions for setting PMT voltages and fuorescence
compensation prior to analysis; autostandardization and
compensation are to be done with CXP software, using Flow-
Check Pro Fluorospheres, Flow-Set Pro Fluorospheres, and
fuorochrome matched CD45 conjugates.
SPECI MEN COLLECTI ON:
Each fow cytometric analysis requires 100 L of whole
blood, bone marrow or single lymphoid cell suspension
(specimens should be with white blood cell counts in
the range of 2-20 x 10
3
cells/L).
Whole Blood and bone marrow may be collected using
EDTA, Heparin or ACD anticoagulants as appropriate
for the specimen.
EDTA collected specimens stained with Solastra Rea-
gents may be prepared within 24 hours. Analysis of
samples must be performed on the same day as sample
staining. Specimens collected with Heparin or ACD may
be prepared within 48 hours of collection. Analysis of
samples must be performed on the same day as sample
staining.
SPECI MEN PREPAR ATI ON:
A pre-wash procedure is required to optimize the staining
of Kappa- and Lambda-Light Chains on cell surface. It is
generalized to the whole panel with the aim to standardize
the preparation and staining procedures. Freedom is given
to the user to choose a bulk wash procedure where the
totality of the specimen is washed at once, or a classical
single tube wash procedure, where specimen aliquots
are washed separately.
originalet.indd 3 10-06-18 15.23.20
PRODUCT OVERVI EW
Flow cytometric analyses are an integral part of the stan-
dard of care in hematopathology. The Solastra 5-Color
Reagent Panel is a comprehensive CE-marked panel
of conjugated-antibody cocktails for the characterization
of hematolymphoid neoplasia by fow cytometry. These
products are intended for identifcation and enumeration
of relevant leukocyte surface molecules and are useful
as an aid in differential diagnosis of patients with certain
abnormal hematology results and/or presence of blasts in
the blood stream, bone marrow and/or lymphoid tissues.
The Solastra 5-Color Reagents are composed of antibodies
directed to B, T, and Myelomonocytic lineage antigens and
refect the minimum number of markers recommended by
the 2006 Bethesda International Consensus conference
published conclusions (1, 2).
MARKERS
The following markers are included in the kits,
respectively:
B Lineage Kit: CD45, CD5, CD10, CD19, CD20,
CD38, Kappa and Lambda
T Lineage Kit: CD45, CD2, CD3, CD4, CD5, CD7,
CD8 and CD56
Myelomonocytic Lineage Kit: CD45, CD7, CD11b,
CD13, CD14, CD15, CD16, CD33, CD34, CD56,
CD117 and HLA-DR
MARKER CLONE REACTI VI TY
CD2 39C1.5
IgG2a (rat)
T cells and most of the NK cells
CD3 UCHT1
IgG1 (mouse)
Mature T cell (cytoplasmic expres-
sion in immature T cells)
CD4 SFCI12T4D11
IgG1 (mouse)
Helper / inducer T cells, monocytes,
immature myeloid cells
CD5 BL1a
IgG2a (mouse)
Thymocytes, mature T cells, subpo-
pulation of B cells
CD7 8H8.1
IgG2a (mouse)
T cells, NK cells, subpopulation of
immature myeloid cells
CD8 SFCI21Thy2D3
IgG1 (mouse)
Cytotoxic / suppressor T cells, sub-
population of NK cells
CD10 ALB1
IgG1 (mouse)
Common acute leukemia antigen
(CALLA), lymphatic precursor cells,
neutrophils, subpopulation of mature
B cells
CD11b Bear1
IgG1 (mouse)
Monocytes, macrophages, neu-
trophils, NK cells
CD13 366 (MY7)
IgG1 (mouse)
Myeloid cells
CD14 RMO52
IgG2a (mouse)
Monocytes, weak expression on
neutrophils
CD15 80H5
IgM (mouse)
Neutrophils, weak expression on
monocytes
CD16 3G8
IgG1 (mouse)
NK cells, neutrophils, subpopulation of
monocytes
CD19 J3-119
IgG1 (mouse)
Precursor and mature B cells
MARKER CLONE REACTI VI TY
CD20 B9E9
IgG2a (mouse)
B cells and a subpopulation of B
precursor cells
CD33 D3HL60.251
IgG1 (mouse)
Monocytes, myeloid precursor cells,
weak on neutrophils
CD34 581
IgG1 (mouse)
Myeloid and lymphoid precursor
cells
CD38 LS198-4-3
IgG1 (mouse)
Activated lymphocytes, subpopula-
tion of B cells, plasma cells
CD45 J.33
IgG1 (mouse)
All leukocytes
CD56 N901
IgG1 (mouse)
NK cells, subpopulation of T cells
CD117 104D2D1
IgG1 (mouse)
Myeloid precursor cells
HLA-DR Immu-357
IgG1 (mouse)
B cells, activated T cells, monocytes,
precursor cells
Kappa N/A: Polyclonal Kappa light chain
Lambda N/A: Polyclonal Lambda light chain
PRODUCTS
The three Beckman Coulter Solastra products (T-, B- and
Myelomonocytic-Lineage Kits) consist of 7 pre-mixed, CE-
IVD, 5-color cocktails. A total of 23 markers are included
as directly conjugated antibodies. As recommended in
the Bethesda International Consensus, CD45 is present
in each combination for gating purposes. Dual parameter
histograms gated on the selected CD45 vs. Side Scatter
populations are then used to determine positively stained
cells for each of the surface antigens recognized by the
antibodies within the kits.
SOL ASTR A 5- COLOR KI TS AND COMBI NATI ONS
PN PANEL TUBE FITC PE ECD PC5.5 PC7
A66286

Solastra B
Lineage Kit
BL1 Kappa Lambda CD19 CD5 CD45
BL2 CD20 CD10 CD19 CD38 CD45
A66287

Solastra T
Lineage Kit
TL1 CD2 CD56 CD7 CD5 CD45
TL2 CD8 CD4 - CD3 CD45
A66288

Solastra
Myelomo-
nocytic
Lineage Kit
ML1 CD15 CD11b CD16 CD14 CD45
ML2 HLA-DR CD56 CD34 CD117 CD45
ML3 CD7 CD13 CD34 CD33 CD45
STOR AGE CONDI TI ONS AND STABI LI T Y:
Closed vial stability: 9 months maximum after date of manu-
facturing when stored in standard laboratory refrigerators,
i.e. at 2 8C. Do not freeze. Opened vials are stable for
90 days, provided that care is taken to minimize exposure
to light and return to 2 8C immediately after use.
originalet.indd 2 10-06-18 15.23.14
PRODUCT OVERVI EW
Flow cytometric analyses are an integral part of the stan-
dard of care in hematopathology. The Solastra 5-Color
Reagent Panel is a comprehensive CE-marked panel
of conjugated-antibody cocktails for the characterization
of hematolymphoid neoplasia by fow cytometry. These
products are intended for identifcation and enumeration
of relevant leukocyte surface molecules and are useful
as an aid in differential diagnosis of patients with certain
abnormal hematology results and/or presence of blasts in
the blood stream, bone marrow and/or lymphoid tissues.
The Solastra 5-Color Reagents are composed of antibodies
directed to B, T, and Myelomonocytic lineage antigens and
refect the minimum number of markers recommended by
the 2006 Bethesda International Consensus conference
published conclusions (1, 2).
MARKERS
The following markers are included in the kits,
respectively:
B Lineage Kit: CD45, CD5, CD10, CD19, CD20,
CD38, Kappa and Lambda
T Lineage Kit: CD45, CD2, CD3, CD4, CD5, CD7,
CD8 and CD56
Myelomonocytic Lineage Kit: CD45, CD7, CD11b,
CD13, CD14, CD15, CD16, CD33, CD34, CD56,
CD117 and HLA-DR
MARKER CLONE REACTI VI TY
CD2 39C1.5
IgG2a (rat)
T cells and most of the NK cells
CD3 UCHT1
IgG1 (mouse)
Mature T cell (cytoplasmic expres-
sion in immature T cells)
CD4 SFCI12T4D11
IgG1 (mouse)
Helper / inducer T cells, monocytes,
immature myeloid cells
CD5 BL1a
IgG2a (mouse)
Thymocytes, mature T cells, subpo-
pulation of B cells
CD7 8H8.1
IgG2a (mouse)
T cells, NK cells, subpopulation of
immature myeloid cells
CD8 SFCI21Thy2D3
IgG1 (mouse)
Cytotoxic / suppressor T cells, sub-
population of NK cells
CD10 ALB1
IgG1 (mouse)
Common acute leukemia antigen
(CALLA), lymphatic precursor cells,
neutrophils, subpopulation of mature
B cells
CD11b Bear1
IgG1 (mouse)
Monocytes, macrophages, neu-
trophils, NK cells
CD13 366 (MY7)
IgG1 (mouse)
Myeloid cells
CD14 RMO52
IgG2a (mouse)
Monocytes, weak expression on
neutrophils
CD15 80H5
IgM (mouse)
Neutrophils, weak expression on
monocytes
CD16 3G8
IgG1 (mouse)
NK cells, neutrophils, subpopulation of
monocytes
CD19 J3-119
IgG1 (mouse)
Precursor and mature B cells
MARKER CLONE REACTI VI TY
CD20 B9E9
IgG2a (mouse)
B cells and a subpopulation of B
precursor cells
CD33 D3HL60.251
IgG1 (mouse)
Monocytes, myeloid precursor cells,
weak on neutrophils
CD34 581
IgG1 (mouse)
Myeloid and lymphoid precursor
cells
CD38 LS198-4-3
IgG1 (mouse)
Activated lymphocytes, subpopula-
tion of B cells, plasma cells
CD45 J.33
IgG1 (mouse)
All leukocytes
CD56 N901
IgG1 (mouse)
NK cells, subpopulation of T cells
CD117 104D2D1
IgG1 (mouse)
Myeloid precursor cells
HLA-DR Immu-357
IgG1 (mouse)
B cells, activated T cells, monocytes,
precursor cells
Kappa N/A: Polyclonal Kappa light chain
Lambda N/A: Polyclonal Lambda light chain
PRODUCTS
The three Beckman Coulter Solastra products (T-, B- and
Myelomonocytic-Lineage Kits) consist of 7 pre-mixed, CE-
IVD, 5-color cocktails. A total of 23 markers are included
as directly conjugated antibodies. As recommended in
the Bethesda International Consensus, CD45 is present
in each combination for gating purposes. Dual parameter
histograms gated on the selected CD45 vs. Side Scatter
populations are then used to determine positively stained
cells for each of the surface antigens recognized by the
antibodies within the kits.
SOL ASTR A 5- COLOR KI TS AND COMBI NATI ONS
PN PANEL TUBE FITC PE ECD PC5.5 PC7
A66286
Solastra B
Lineage Kit
BL1 Kappa Lambda CD19 CD5 CD45
BL2 CD20 CD10 CD19 CD38 CD45
A66287
Solastra T
Lineage Kit
TL1 CD2 CD56 CD7 CD5 CD45
TL2 CD8 CD4 - CD3 CD45
A66288
Solastra
Myelomo-
nocytic
Lineage Kit
ML1 CD15 CD11b CD16 CD14 CD45
ML2 HLA-DR CD56 CD34 CD117 CD45
ML3 CD7 CD13 CD34 CD33 CD45
STOR AGE CONDI TI ONS AND STABI LI T Y:
Closed vial stability: 9 months maximum after date of manu-
facturing when stored in standard laboratory refrigerators,
i.e. at 2 8C. Do not freeze. Opened vials are stable for
90 days, provided that care is taken to minimize exposure
to light and return to 2 8C immediately after use.
originalet.indd 2 10-06-18 15.23.14
MARKER CLONE REACTI VI TY
CD20 B9E9
IgG2a (mouse)
B cells and a subpopulation of B
precursor cells
CD33 D3HL60.251
IgG1 (mouse)
Monocytes, myeloid precursor cells,
weak on neutrophils
CD34 581
IgG1 (mouse)
Myeloid and lymphoid precursor
cells
CD38 LS198-4-3
IgG1 (mouse)
Activated lymphocytes, subpopula-
tion of B cells, plasma cells
CD45 J.33
IgG1 (mouse)
All leukocytes
CD56 N901
IgG1 (mouse)
NK cells, subpopulation of T cells
CD117 104D2D1
IgG1 (mouse)
Myeloid precursor cells
HLA-DR Immu-357
IgG1 (mouse)
B cells, activated T cells, monocytes,
precursor cells
Kappa N/A: Polyclonal Kappa light chain
Lambda N/A: Polyclonal Lambda light chain
PRODUCTS
The three Beckman Coulter Solastra products (T-, B- and
Myelomonocytic-Lineage Kits) consist of 7 pre-mixed, CE-
IVD, 5-color cocktails. A total of 23 markers are included
as directly conjugated antibodies. As recommended in
the Bethesda International Consensus, CD45 is present
in each combination for gating purposes. Dual parameter
histograms gated on the selected CD45 vs. Side Scatter
populations are then used to determine positively stained
cells for each of the surface antigens recognized by the
antibodies within the kits.
SOL ASTR A 5- COLOR KI TS AND COMBI NATI ONS
PN PANEL TUBE FITC PE ECD PC5.5 PC7
A66286
Solastra B
Lineage Kit
BL1 Kappa Lambda CD19 CD5 CD45
BL2 CD20 CD10 CD19 CD38 CD45
A66287
Solastra T
Lineage Kit
TL1 CD2 CD56 CD7 CD5 CD45
TL2 CD8 CD4 - CD3 CD45
A66288
Solastra
Myelomo-
nocytic
Lineage Kit
ML1 CD15 CD11b CD16 CD14 CD45
ML2 HLA-DR CD56 CD34 CD117 CD45
ML3 CD7 CD13 CD34 CD33 CD45
STOR AGE CONDI TI ONS AND STABI LI T Y:
Closed vial stability: 9 months maximum after date of manu-
facturing when stored in standard laboratory refrigerators,
i.e. at 2 8C. Do not freeze. Opened vials are stable for
90 days, provided that care is taken to minimize exposure
to light and return to 2 8C immediately after use.
RE AGENTS REQUI RED BUT NOT SUPPLI ED
VersaLyse Lysing Solution, PN A09777
IOTest 3 Fixative Solution, PN A07800
Flow-Check Pro Fluorospheres, PN A63493
Flow-Set Pro Fluorospheres, PN A63492
Solastra QuickCOMP 5 Kit, PN A83571

or
QuickCOMP 4 Kit (except for CD45-PC5),
PN 177017, with CD45-PC5.5, PN A62835, and
CD45-PC7, PN IM3548
Phosphate Buffered Saline (PBS), PN 6603369
Heat-Inactivated Fetal Calf Serum (HIFCS)
Heat-Inactivated Mouse Serum (HIMS) (facultative)
FLOW CY TOMETERS AND FLUOROCHROMES:
Solastra is well adapted to Beckman Coulter Cytomics FC
500 Flow Cytometers, capable of simultaneous 5-color
analysis from a single 488 nm laser line. Fluorochromes
are usable without flter change on the FC 500 confgured
with the standard IVD application flter set for 5-color single
laser analysis. If necessary, optical bench can be adapted
to the PC5.5 fuorochrome emission spectrum by using
the following devices: Single Laser Filter Kit for FC 500
fow cytometer, PN 179044 or Single Laser Filter Block
Assembly for FC 500 fow cytometer, PN 179045.
FLUOROCHROME PRIMARY
EXCITATION (nm)
MAXIMUM
EMISSION (nm)
FITC 468-509 504-541
PE 486-580 568-590
ECD 486-580 610-635
PC5.5 486-580 680-710
PC7 486-580 750-790
I NSTRUMENT SET TI NGS:
Users should refer to the instruments manuals for speci-
fc instructions for setting PMT voltages and fuorescence
compensation prior to analysis; autostandardization and
compensation are to be done with CXP software, using Flow-
Check Pro Fluorospheres, Flow-Set Pro Fluorospheres, and
fuorochrome matched CD45 conjugates.
SPECI MEN COLLECTI ON:
Each fow cytometric analysis requires 100 L of whole
blood, bone marrow or single lymphoid cell suspension
(specimens should be with white blood cell counts in
the range of 2-20 x 10
3
cells/L).
Whole Blood and bone marrow may be collected using
EDTA, Heparin or ACD anticoagulants as appropriate
for the specimen.
EDTA collected specimens stained with Solastra Rea-
gents may be prepared within 24 hours. Analysis of
samples must be performed on the same day as sample
staining. Specimens collected with Heparin or ACD may
be prepared within 48 hours of collection. Analysis of
samples must be performed on the same day as sample
staining.
SPECI MEN PREPAR ATI ON:
A pre-wash procedure is required to optimize the staining
of Kappa- and Lambda-Light Chains on cell surface. It is
generalized to the whole panel with the aim to standardize
the preparation and staining procedures. Freedom is given
to the user to choose a bulk wash procedure where the
totality of the specimen is washed at once, or a classical
single tube wash procedure, where specimen aliquots
are washed separately.
originalet.indd 3 10-06-18 15.23.20
BULK WASH PROCEDURE
1. Obtain WBC count of the sample.
2. Add 1.0 mL whole blood or bone marrow specimen
to a 15 mL conical centrifuge tube.
3. Add no less than 9.0 mL of the PBS / 2% FCS wash
buffer (1:10 dilution is critical).
Mix by gentle inversion.
4. Centrifuge at 150 x g for 10 minutes at room tempe-
rature (20 25C).
5. Aspirate (do not decant) and discard
supernatant.
6. Repeat steps 3-5 two additional times.
7. Resuspend the washed pellet in either PBS / 2%
HIFCS or PBS / 50% HIMS with an appropriate vo-
lume to obtain a WBC count of 2-20 x 10
3
cells/L.
8. Proceed to Staining Procedure.
OR: SI NGLE TUBE WASH PROCEDURE
1. Obtain WBC count of the sample.
a. If the WBC count is above 20 x 10
3
cells/L, di-
lute sample appropriately with the PBS/2% FCS
wash buffer.
b. If the WBC count is <2 x 10
3
cells/L, the sample
must be concentrated prior to washing.
2. For each sample add 100 L of whole blood or bone
marrow specimen to three 12 x 75 mm test tubes labe-
led for each of the Solastra Lineage Reagents used.
3. Add 3.0 mL of the PBS/2% FCS wash buffer. Mix by
gentle inversion.
4. Centrifuge at 1000 x g for 2 minutes.
5. Aspirate and discard supernatant.
6. Repeat steps 3-5 two additional times.
7. Resuspend the washed pellet in either PBS / 2%
HIFCS or PBS / 50% HIMS to the initial 100 L vo-
lume.
8. Proceed to Staining Procedure, Step 3.
STAI NI NG PROCEDURE:

1. For each sample washed using the Bulk Wash
Procedure, and for the single cell suspensions of
lymphoid tissues, label individual 12 x 75 mm test
tubes. For samples washed using the Single Tube
Wash Procedure, proceed to Step 3.
2. Add 100 L of the sample to each test tube.
3. Add 20 L of Solastra reagents to the corresponding
labeled test tube. Vortex gently.
4. Incubate the reaction mixtures at 20-25C for 15-20
minutes. Protect from light.
5. Lyse the red blood cells in each test tube
NOTE: Single cell suspensions from lymphoid tis-
sues do not require the red blood cell lysis.
Proceed to step e.
a. Add 1 mL of the VersaLyse Fix-and-Lyse
mixture to each test tube and vortex imme-
diately for 1 second.
b. Incubate at least 10 minutes at room tempe-
rature (20 25C), protected from light.
c. Centrifuge for 5 minutes at 150 x g at room
temperature.
d. Remove the supernatant by aspiration.
e. Resuspend the cell pellet in 3 mL of PBS.
f. Centrifuge for 5 minutes at 150 x g at room
temperature.
g. Remove the supernatant by aspiration and
resuspend the cell pellet in 0.5 mL of 0.1%
formaldehyde PBS buffer.
h. To minimize the possibility of less than opti-
mal results, analyze stained cells promptly.
6. Analyze cells on a fow cytometer properly standar-
dized and gated on each population of interest.
LOWER LI MI T OF DETECTI ON:
A study was conducted in accordance with CLSI Approved
Guidelines (4). Results support a lower limit of detection
of 0.3% when collecting 50,000 events.
E XPECTED VALUES:
These are intended as representative values only. Each
laboratory should establish its own expected values from
the local population of normal donors.
SOL ASTR A B LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD19+ (BL1 Tube) 127 10.90 2.98 20.28
CD19+ (BL2 Tube) 127 11.06 2.87 21.43
CD20+ 127 10.87 2.85 21.41
CD5+ 130 79.40 63.10 93.27
% CD19+ B Lymphocytes
CD19+Kappa+ 83 58.24 43.33 75.95
CD19+Lambda+ 83 38.55 30.42 47.55
% Monocytes
CD38+ 130 95.25 79.19 99.66
% Granulocytes
CD10+ 129 93.73 70.94 99.96
originalet.indd 4 10-06-18 15.23.23
BULK WASH PROCEDURE
1. Obtain WBC count of the sample.
2. Add 1.0 mL whole blood or bone marrow specimen
to a 15 mL conical centrifuge tube.
3. Add no less than 9.0 mL of the PBS / 2% FCS wash
buffer (1:10 dilution is critical).
Mix by gentle inversion.
4. Centrifuge at 150 x g for 10 minutes at room tempe-
rature (20 25C).
5. Aspirate (do not decant) and discard
supernatant.
6. Repeat steps 3-5 two additional times.
7. Resuspend the washed pellet in either PBS / 2%
HIFCS or PBS / 50% HIMS with an appropriate vo-
lume to obtain a WBC count of 2-20 x 10
3
cells/L.
8. Proceed to Staining Procedure.
OR: SI NGLE TUBE WASH PROCEDURE
1. Obtain WBC count of the sample.
a. If the WBC count is above 20 x 10
3
cells/L, di-
lute sample appropriately with the PBS/2% FCS
wash buffer.
b. If the WBC count is <2 x 10
3
cells/L, the sample
must be concentrated prior to washing.
2. For each sample add 100 L of whole blood or bone
marrow specimen to three 12 x 75 mm test tubes labe-
led for each of the Solastra Lineage Reagents used.
3. Add 3.0 mL of the PBS/2% FCS wash buffer. Mix by
gentle inversion.
4. Centrifuge at 1000 x g for 2 minutes.
5. Aspirate and discard supernatant.
6. Repeat steps 3-5 two additional times.
7. Resuspend the washed pellet in either PBS / 2%
HIFCS or PBS / 50% HIMS to the initial 100 L vo-
lume.
8. Proceed to Staining Procedure, Step 3.
STAI NI NG PROCEDURE:

1. For each sample washed using the Bulk Wash
Procedure, and for the single cell suspensions of
lymphoid tissues, label individual 12 x 75 mm test
tubes. For samples washed using the Single Tube
Wash Procedure, proceed to Step 3.
2. Add 100 L of the sample to each test tube.
3. Add 20 L of Solastra reagents to the corresponding
labeled test tube. Vortex gently.
4. Incubate the reaction mixtures at 20-25C for 15-20
minutes. Protect from light.
5. Lyse the red blood cells in each test tube
NOTE: Single cell suspensions from lymphoid tis-
sues do not require the red blood cell lysis.
Proceed to step e.
a. Add 1 mL of the VersaLyse Fix-and-Lyse
mixture to each test tube and vortex imme-
diately for 1 second.
b. Incubate at least 10 minutes at room tempe-
rature (20 25C), protected from light.
c. Centrifuge for 5 minutes at 150 x g at room
temperature.
d. Remove the supernatant by aspiration.
e. Resuspend the cell pellet in 3 mL of PBS.
f. Centrifuge for 5 minutes at 150 x g at room
temperature.
g. Remove the supernatant by aspiration and
resuspend the cell pellet in 0.5 mL of 0.1%
formaldehyde PBS buffer.
h. To minimize the possibility of less than opti-
mal results, analyze stained cells promptly.
6. Analyze cells on a fow cytometer properly standar-
dized and gated on each population of interest.
LOWER LI MI T OF DETECTI ON:
A study was conducted in accordance with CLSI Approved
Guidelines (4). Results support a lower limit of detection
of 0.3% when collecting 50,000 events.
E XPECTED VALUES:
These are intended as representative values only. Each
laboratory should establish its own expected values from
the local population of normal donors.
SOL ASTR A B LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD19+ (BL1 Tube) 127 10.90 2.98 20.28
CD19+ (BL2 Tube) 127 11.06 2.87 21.43
CD20+ 127 10.87 2.85 21.41
CD5+ 130 79.40 63.10 93.27
% CD19+ B Lymphocytes
CD19+Kappa+ 83 58.24 43.33 75.95
CD19+Lambda+ 83 38.55 30.42 47.55
% Monocytes
CD38+ 130 95.25 79.19 99.66
% Granulocytes
CD10+ 129 93.73 70.94 99.96
originalet.indd 4 10-06-18 15.23.23
MARKER CLONE REACTI VI TY
CD20 B9E9
IgG2a (mouse)
B cells and a subpopulation of B
precursor cells
CD33 D3HL60.251
IgG1 (mouse)
Monocytes, myeloid precursor cells,
weak on neutrophils
CD34 581
IgG1 (mouse)
Myeloid and lymphoid precursor
cells
CD38 LS198-4-3
IgG1 (mouse)
Activated lymphocytes, subpopula-
tion of B cells, plasma cells
CD45 J.33
IgG1 (mouse)
All leukocytes
CD56 N901
IgG1 (mouse)
NK cells, subpopulation of T cells
CD117 104D2D1
IgG1 (mouse)
Myeloid precursor cells
HLA-DR Immu-357
IgG1 (mouse)
B cells, activated T cells, monocytes,
precursor cells
Kappa N/A: Polyclonal Kappa light chain
Lambda N/A: Polyclonal Lambda light chain
PRODUCTS
The three Beckman Coulter Solastra products (T-, B- and
Myelomonocytic-Lineage Kits) consist of 7 pre-mixed, CE-
IVD, 5-color cocktails. A total of 23 markers are included
as directly conjugated antibodies. As recommended in
the Bethesda International Consensus, CD45 is present
in each combination for gating purposes. Dual parameter
histograms gated on the selected CD45 vs. Side Scatter
populations are then used to determine positively stained
cells for each of the surface antigens recognized by the
antibodies within the kits.
SOL ASTR A 5- COLOR KI TS AND COMBI NATI ONS
PN PANEL TUBE FITC PE ECD PC5.5 PC7
A66286
Solastra B
Lineage Kit
BL1 Kappa Lambda CD19 CD5 CD45
BL2 CD20 CD10 CD19 CD38 CD45
A66287
Solastra T
Lineage Kit
TL1 CD2 CD56 CD7 CD5 CD45
TL2 CD8 CD4 - CD3 CD45
A66288
Solastra
Myelomo-
nocytic
Lineage Kit
ML1 CD15 CD11b CD16 CD14 CD45
ML2 HLA-DR CD56 CD34 CD117 CD45
ML3 CD7 CD13 CD34 CD33 CD45
STOR AGE CONDI TI ONS AND STABI LI T Y:
Closed vial stability: 9 months maximum after date of manu-
facturing when stored in standard laboratory refrigerators,
i.e. at 2 8C. Do not freeze. Opened vials are stable for
90 days, provided that care is taken to minimize exposure
to light and return to 2 8C immediately after use.
RE AGENTS REQUI RED BUT NOT SUPPLI ED
VersaLyse Lysing Solution, PN A09777
IOTest 3 Fixative Solution, PN A07800
Flow-Check Pro Fluorospheres, PN A63493
Flow-Set Pro Fluorospheres, PN A63492
Solastra QuickCOMP 5 Kit, PN A83571

or
QuickCOMP 4 Kit (except for CD45-PC5),
PN 177017, with CD45-PC5.5, PN A62835, and
CD45-PC7, PN IM3548
Phosphate Buffered Saline (PBS), PN 6603369
Heat-Inactivated Fetal Calf Serum (HIFCS)
Heat-Inactivated Mouse Serum (HIMS) (facultative)
FLOW CY TOMETERS AND FLUOROCHROMES:
Solastra is well adapted to Beckman Coulter Cytomics FC
500 Flow Cytometers, capable of simultaneous 5-color
analysis from a single 488 nm laser line. Fluorochromes
are usable without flter change on the FC 500 confgured
with the standard IVD application flter set for 5-color single
laser analysis. If necessary, optical bench can be adapted
to the PC5.5 fuorochrome emission spectrum by using
the following devices: Single Laser Filter Kit for FC 500
fow cytometer, PN 179044 or Single Laser Filter Block
Assembly for FC 500 fow cytometer, PN 179045.
FLUOROCHROME PRIMARY
EXCITATION (nm)
MAXIMUM
EMISSION (nm)
FITC 468-509 504-541
PE 486-580 568-590
ECD 486-580 610-635
PC5.5 486-580 680-710
PC7 486-580 750-790
I NSTRUMENT SET TI NGS:
Users should refer to the instruments manuals for speci-
fc instructions for setting PMT voltages and fuorescence
compensation prior to analysis; autostandardization and
compensation are to be done with CXP software, using Flow-
Check Pro Fluorospheres, Flow-Set Pro Fluorospheres, and
fuorochrome matched CD45 conjugates.
SPECI MEN COLLECTI ON:
Each fow cytometric analysis requires 100 L of whole
blood, bone marrow or single lymphoid cell suspension
(specimens should be with white blood cell counts in
the range of 2-20 x 10
3
cells/L).
Whole Blood and bone marrow may be collected using
EDTA, Heparin or ACD anticoagulants as appropriate
for the specimen.
EDTA collected specimens stained with Solastra Rea-
gents may be prepared within 24 hours. Analysis of
samples must be performed on the same day as sample
staining. Specimens collected with Heparin or ACD may
be prepared within 48 hours of collection. Analysis of
samples must be performed on the same day as sample
staining.
SPECI MEN PREPAR ATI ON:
A pre-wash procedure is required to optimize the staining
of Kappa- and Lambda-Light Chains on cell surface. It is
generalized to the whole panel with the aim to standardize
the preparation and staining procedures. Freedom is given
to the user to choose a bulk wash procedure where the
totality of the specimen is washed at once, or a classical
single tube wash procedure, where specimen aliquots
are washed separately.
originalet.indd 3 10-06-18 15.23.20
BULK WASH PROCEDURE
1. Obtain WBC count of the sample.
2. Add 1.0 mL whole blood or bone marrow specimen
to a 15 mL conical centrifuge tube.
3. Add no less than 9.0 mL of the PBS / 2% FCS wash
buffer (1:10 dilution is critical).
Mix by gentle inversion.
4. Centrifuge at 150 x g for 10 minutes at room tempe-
rature (20 25C).
5. Aspirate (do not decant) and discard
supernatant.
6. Repeat steps 3-5 two additional times.
7. Resuspend the washed pellet in either PBS / 2%
HIFCS or PBS / 50% HIMS with an appropriate vo-
lume to obtain a WBC count of 2-20 x 10
3
cells/L.
8. Proceed to Staining Procedure.
OR: SI NGLE TUBE WASH PROCEDURE
1. Obtain WBC count of the sample.
a. If the WBC count is above 20 x 10
3
cells/L, di-
lute sample appropriately with the PBS/2% FCS
wash buffer.
b. If the WBC count is <2 x 10
3
cells/L, the sample
must be concentrated prior to washing.
2. For each sample add 100 L of whole blood or bone
marrow specimen to three 12 x 75 mm test tubes labe-
led for each of the Solastra Lineage Reagents used.
3. Add 3.0 mL of the PBS/2% FCS wash buffer. Mix by
gentle inversion.
4. Centrifuge at 1000 x g for 2 minutes.
5. Aspirate and discard supernatant.
6. Repeat steps 3-5 two additional times.
7. Resuspend the washed pellet in either PBS / 2%
HIFCS or PBS / 50% HIMS to the initial 100 L vo-
lume.
8. Proceed to Staining Procedure, Step 3.
STAI NI NG PROCEDURE:

1. For each sample washed using the Bulk Wash
Procedure, and for the single cell suspensions of
lymphoid tissues, label individual 12 x 75 mm test
tubes. For samples washed using the Single Tube
Wash Procedure, proceed to Step 3.
2. Add 100 L of the sample to each test tube.
3. Add 20 L of Solastra reagents to the corresponding
labeled test tube. Vortex gently.
4. Incubate the reaction mixtures at 20-25C for 15-20
minutes. Protect from light.
5. Lyse the red blood cells in each test tube
NOTE: Single cell suspensions from lymphoid tis-
sues do not require the red blood cell lysis.
Proceed to step e.
a. Add 1 mL of the VersaLyse Fix-and-Lyse
mixture to each test tube and vortex imme-
diately for 1 second.
b. Incubate at least 10 minutes at room tempe-
rature (20 25C), protected from light.
c. Centrifuge for 5 minutes at 150 x g at room
temperature.
d. Remove the supernatant by aspiration.
e. Resuspend the cell pellet in 3 mL of PBS.
f. Centrifuge for 5 minutes at 150 x g at room
temperature.
g. Remove the supernatant by aspiration and
resuspend the cell pellet in 0.5 mL of 0.1%
formaldehyde PBS buffer.
h. To minimize the possibility of less than opti-
mal results, analyze stained cells promptly.
6. Analyze cells on a fow cytometer properly standar-
dized and gated on each population of interest.
LOWER LI MI T OF DETECTI ON:
A study was conducted in accordance with CLSI Approved
Guidelines (4). Results support a lower limit of detection
of 0.3% when collecting 50,000 events.
E XPECTED VALUES:
These are intended as representative values only. Each
laboratory should establish its own expected values from
the local population of normal donors.
SOL ASTR A B LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD19+ (BL1 Tube) 127 10.90 2.98 20.28
CD19+ (BL2 Tube) 127 11.06 2.87 21.43
CD20+ 127 10.87 2.85 21.41
CD5+ 130 79.40 63.10 93.27
% CD19+ B Lymphocytes
CD19+Kappa+ 83 58.24 43.33 75.95
CD19+Lambda+ 83 38.55 30.42 47.55
% Monocytes
CD38+ 130 95.25 79.19 99.66
% Granulocytes
CD10+ 129 93.73 70.94 99.96
originalet.indd 4 10-06-18 15.23.23
BULK WASH PROCEDURE
1. Obtain WBC count of the sample.
2. Add 1.0 mL whole blood or bone marrow specimen
to a 15 mL conical centrifuge tube.
3. Add no less than 9.0 mL of the PBS / 2% FCS wash
buffer (1:10 dilution is critical).
Mix by gentle inversion.
4. Centrifuge at 150 x g for 10 minutes at room tempe-
rature (20 25C).
5. Aspirate (do not decant) and discard
supernatant.
6. Repeat steps 3-5 two additional times.
7. Resuspend the washed pellet in either PBS / 2%
HIFCS or PBS / 50% HIMS with an appropriate vo-
lume to obtain a WBC count of 2-20 x 10
3
cells/L.
8. Proceed to Staining Procedure.
OR: SI NGLE TUBE WASH PROCEDURE
1. Obtain WBC count of the sample.
a. If the WBC count is above 20 x 10
3
cells/L, di-
lute sample appropriately with the PBS/2% FCS
wash buffer.
b. If the WBC count is <2 x 10
3
cells/L, the sample
must be concentrated prior to washing.
2. For each sample add 100 L of whole blood or bone
marrow specimen to three 12 x 75 mm test tubes labe-
led for each of the Solastra Lineage Reagents used.
3. Add 3.0 mL of the PBS/2% FCS wash buffer. Mix by
gentle inversion.
4. Centrifuge at 1000 x g for 2 minutes.
5. Aspirate and discard supernatant.
6. Repeat steps 3-5 two additional times.
7. Resuspend the washed pellet in either PBS / 2%
HIFCS or PBS / 50% HIMS to the initial 100 L vo-
lume.
8. Proceed to Staining Procedure, Step 3.
STAI NI NG PROCEDURE:

1. For each sample washed using the Bulk Wash
Procedure, and for the single cell suspensions of
lymphoid tissues, label individual 12 x 75 mm test
tubes. For samples washed using the Single Tube
Wash Procedure, proceed to Step 3.
2. Add 100 L of the sample to each test tube.
3. Add 20 L of Solastra reagents to the corresponding
labeled test tube. Vortex gently.
4. Incubate the reaction mixtures at 20-25C for 15-20
minutes. Protect from light.
5. Lyse the red blood cells in each test tube
NOTE: Single cell suspensions from lymphoid tis-
sues do not require the red blood cell lysis.
Proceed to step e.
a. Add 1 mL of the VersaLyse Fix-and-Lyse
mixture to each test tube and vortex imme-
diately for 1 second.
b. Incubate at least 10 minutes at room tempe-
rature (20 25C), protected from light.
c. Centrifuge for 5 minutes at 150 x g at room
temperature.
d. Remove the supernatant by aspiration.
e. Resuspend the cell pellet in 3 mL of PBS.
f. Centrifuge for 5 minutes at 150 x g at room
temperature.
g. Remove the supernatant by aspiration and
resuspend the cell pellet in 0.5 mL of 0.1%
formaldehyde PBS buffer.
h. To minimize the possibility of less than opti-
mal results, analyze stained cells promptly.
6. Analyze cells on a fow cytometer properly standar-
dized and gated on each population of interest.
LOWER LI MI T OF DETECTI ON:
A study was conducted in accordance with CLSI Approved
Guidelines (4). Results support a lower limit of detection
of 0.3% when collecting 50,000 events.
E XPECTED VALUES:
These are intended as representative values only. Each
laboratory should establish its own expected values from
the local population of normal donors.
SOL ASTR A B LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD19+ (BL1 Tube) 127 10.90 2.98 20.28
CD19+ (BL2 Tube) 127 11.06 2.87 21.43
CD20+ 127 10.87 2.85 21.41
CD5+ 130 79.40 63.10 93.27
% CD19+ B Lymphocytes
CD19+Kappa+ 83 58.24 43.33 75.95
CD19+Lambda+ 83 38.55 30.42 47.55
% Monocytes
CD38+ 130 95.25 79.19 99.66
% Granulocytes
CD10+ 129 93.73 70.94 99.96
originalet.indd 4 10-06-18 15.23.23
REPRESENTATI VE RESULTS:
Example of a mature B-cell neoplasm bone marrow
aspirate sample.
Figure 1 (upper left): CD45-PC7 vs. SS Histogram
(Ungated). Gate Lymph displays a large CD45
bright

lymphocytic population.
Figure 2 (middle): Tube BL1 gated on Leukocytes
(Gate WBC).
Figure 3 (lower right): Tube BL1 gated on CD19+
lymphocytes (Gate CD19+ Ly).
Figure 4 (right): Tube BL2 gated on Lymphocytes
(Gate CD19+ Ly).
originalet.indd 6 10-06-18 15.23.26
REPRESENTATI VE RESULTS:
Example of a mature B-cell neoplasm bone marrow
aspirate sample.
Figure 1 (upper left): CD45-PC7 vs. SS Histogram
(Ungated). Gate Lymph displays a large CD45
bright

lymphocytic population.
Figure 2 (middle): Tube BL1 gated on Leukocytes
(Gate WBC).
Figure 3 (lower right): Tube BL1 gated on CD19+
lymphocytes (Gate CD19+ Ly).
Figure 4 (right): Tube BL2 gated on Lymphocytes
(Gate CD19+ Ly).
originalet.indd 6 10-06-18 15.23.26
BULK WASH PROCEDURE
1. Obtain WBC count of the sample.
2. Add 1.0 mL whole blood or bone marrow specimen
to a 15 mL conical centrifuge tube.
3. Add no less than 9.0 mL of the PBS / 2% FCS wash
buffer (1:10 dilution is critical).
Mix by gentle inversion.
4. Centrifuge at 150 x g for 10 minutes at room tempe-
rature (20 25C).
5. Aspirate (do not decant) and discard
supernatant.
6. Repeat steps 3-5 two additional times.
7. Resuspend the washed pellet in either PBS / 2%
HIFCS or PBS / 50% HIMS with an appropriate vo-
lume to obtain a WBC count of 2-20 x 10
3
cells/L.
8. Proceed to Staining Procedure.
OR: SI NGLE TUBE WASH PROCEDURE
1. Obtain WBC count of the sample.
a. If the WBC count is above 20 x 10
3
cells/L, di-
lute sample appropriately with the PBS/2% FCS
wash buffer.
b. If the WBC count is <2 x 10
3
cells/L, the sample
must be concentrated prior to washing.
2. For each sample add 100 L of whole blood or bone
marrow specimen to three 12 x 75 mm test tubes labe-
led for each of the Solastra Lineage Reagents used.
3. Add 3.0 mL of the PBS/2% FCS wash buffer. Mix by
gentle inversion.
4. Centrifuge at 1000 x g for 2 minutes.
5. Aspirate and discard supernatant.
6. Repeat steps 3-5 two additional times.
7. Resuspend the washed pellet in either PBS / 2%
HIFCS or PBS / 50% HIMS to the initial 100 L vo-
lume.
8. Proceed to Staining Procedure, Step 3.
STAI NI NG PROCEDURE:

1. For each sample washed using the Bulk Wash
Procedure, and for the single cell suspensions of
lymphoid tissues, label individual 12 x 75 mm test
tubes. For samples washed using the Single Tube
Wash Procedure, proceed to Step 3.
2. Add 100 L of the sample to each test tube.
3. Add 20 L of Solastra reagents to the corresponding
labeled test tube. Vortex gently.
4. Incubate the reaction mixtures at 20-25C for 15-20
minutes. Protect from light.
5. Lyse the red blood cells in each test tube
NOTE: Single cell suspensions from lymphoid tis-
sues do not require the red blood cell lysis.
Proceed to step e.
a. Add 1 mL of the VersaLyse Fix-and-Lyse
mixture to each test tube and vortex imme-
diately for 1 second.
b. Incubate at least 10 minutes at room tempe-
rature (20 25C), protected from light.
c. Centrifuge for 5 minutes at 150 x g at room
temperature.
d. Remove the supernatant by aspiration.
e. Resuspend the cell pellet in 3 mL of PBS.
f. Centrifuge for 5 minutes at 150 x g at room
temperature.
g. Remove the supernatant by aspiration and
resuspend the cell pellet in 0.5 mL of 0.1%
formaldehyde PBS buffer.
h. To minimize the possibility of less than opti-
mal results, analyze stained cells promptly.
6. Analyze cells on a fow cytometer properly standar-
dized and gated on each population of interest.
LOWER LI MI T OF DETECTI ON:
A study was conducted in accordance with CLSI Approved
Guidelines (4). Results support a lower limit of detection
of 0.3% when collecting 50,000 events.
E XPECTED VALUES:
These are intended as representative values only. Each
laboratory should establish its own expected values from
the local population of normal donors.
SOL ASTR A B LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD19+ (BL1 Tube) 127 10.90 2.98 20.28
CD19+ (BL2 Tube) 127 11.06 2.87 21.43
CD20+ 127 10.87 2.85 21.41
CD5+ 130 79.40 63.10 93.27
% CD19+ B Lymphocytes
CD19+Kappa+ 83 58.24 43.33 75.95
CD19+Lambda+ 83 38.55 30.42 47.55
% Monocytes
CD38+ 130 95.25 79.19 99.66
% Granulocytes
CD10+ 129 93.73 70.94 99.96
originalet.indd 4 10-06-18 15.23.23
SOL ASTR A T LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD2+ 130 85.24 72.63 95.35
CD5+ 130 78.65 61.42 88.13
CD7+ 130 81.53 69.74 91.04
CD56+ 130 14.38 3.97 32.34
CD3+ 130 77.82 62.42 88.43
CD3+CD4+ 130 49.52 17.61 70.19
CD3+CD8+ 130 26.68 12.01 52.40
SOL ASTRA MYELOMONOCYTI C LI NEAGE KI T /
NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD7+ 130 81.01 65.80 92.79
CD56+ 129 14.39 4.03 32.92
% Granulocytes
CD11b+ 130 99.66 97.58 100.00
CD13+ 129 99.41 92.36 100.00
CD15+ 130 99.68 97.55 99.99
CD16+ 130 95.48 84.59 99.68
% Monocytes
CD14+ 129 96.94 90.18 99.46
CD33+ 129 97.90 90.73 99.70
HLA-DR+ 130 98.40 88.69 99.95
PRECI SI ON:
The percent positive values were determined using IM-
MUNO-TROL (or, as necessary, IMMUNO-TROL with
Stem-Trol Control Cells, and IMMUNO-TROL with MO7E
cell line), run in duplicate, twice each day for up to 20 days
at 4 geographically diverse sites using the Solastra B, T
and Myelomonocytic Lineage Kit reagents.
SOL ASTR A B LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD19+ (BL1 Tube) 2.80% 14.20
CD19+ (BL2 Tube) 2.74% 14.34
CD20+ 3.56% 14.78
CD5+ 1.04% 71.86
% CD19 B Lymphocytes
CD19+Kappa+ 2.91% 57.42
CD19+Lambda+ 4.06% 40.23
% CD38 Monocytes
CD38+ 0.89% 94.45
% CD10+ Granulocytes
CD10+ 0.03% 99.96
SOL ASTR A T LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD2+ 0.67% 81.78
CD5+ 0.93% 72.73
CD7+ 0.73% 74.65
CD56+ 3.32% 13.76
CD3+ 0.76% 71.16
CD3+CD4+ 1.24% 45.48
CD3+CD8+ 1.94% 22.67
SOL ASTR A MYELOMONOCY TI C LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD7+ 1.59% 73.88
CD56+ 7.34% 14.16
% Granulocytes
CD11b+ 2.34% 97.26
CD13+ 0.43% 99.91
CD15+ 0.20% 99.76
CD16+ 0.56% 94.56
% Monocytes
CD14+ 0.91% 89.35
CD33+ 0.94% 95.44
HLA-DR+ 0.56% 95.20
% Stem-Trol Population
CD34+ (ML2 Tube) 9.26% 11.85
CD34+ (ML3 Tube) 7.00% 11.75
% MO7E Cell Line Population
CD117+ 9.23% 13.89
originalet.indd 5 10-06-18 15.23.25
SOL ASTR A T LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD2+ 130 85.24 72.63 95.35
CD5+ 130 78.65 61.42 88.13
CD7+ 130 81.53 69.74 91.04
CD56+ 130 14.38 3.97 32.34
CD3+ 130 77.82 62.42 88.43
CD3+CD4+ 130 49.52 17.61 70.19
CD3+CD8+ 130 26.68 12.01 52.40
SOL ASTRA MYELOMONOCYTI C LI NEAGE KI T /
NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD7+ 130 81.01 65.80 92.79
CD56+ 129 14.39 4.03 32.92
% Granulocytes
CD11b+ 130 99.66 97.58 100.00
CD13+ 129 99.41 92.36 100.00
CD15+ 130 99.68 97.55 99.99
CD16+ 130 95.48 84.59 99.68
% Monocytes
CD14+ 129 96.94 90.18 99.46
CD33+ 129 97.90 90.73 99.70
HLA-DR+ 130 98.40 88.69 99.95
PRECI SI ON:
The percent positive values were determined using IM-
MUNO-TROL (or, as necessary, IMMUNO-TROL with
Stem-Trol Control Cells, and IMMUNO-TROL with MO7E
cell line), run in duplicate, twice each day for up to 20 days
at 4 geographically diverse sites using the Solastra B, T
and Myelomonocytic Lineage Kit reagents.
SOL ASTR A B LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD19+ (BL1 Tube) 2.80% 14.20
CD19+ (BL2 Tube) 2.74% 14.34
CD20+ 3.56% 14.78
CD5+ 1.04% 71.86
% CD19 B Lymphocytes
CD19+Kappa+ 2.91% 57.42
CD19+Lambda+ 4.06% 40.23
% CD38 Monocytes
CD38+ 0.89% 94.45
% CD10+ Granulocytes
CD10+ 0.03% 99.96
SOL ASTR A T LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD2+ 0.67% 81.78
CD5+ 0.93% 72.73
CD7+ 0.73% 74.65
CD56+ 3.32% 13.76
CD3+ 0.76% 71.16
CD3+CD4+ 1.24% 45.48
CD3+CD8+ 1.94% 22.67
SOL ASTR A MYELOMONOCY TI C LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD7+ 1.59% 73.88
CD56+ 7.34% 14.16
% Granulocytes
CD11b+ 2.34% 97.26
CD13+ 0.43% 99.91
CD15+ 0.20% 99.76
CD16+ 0.56% 94.56
% Monocytes
CD14+ 0.91% 89.35
CD33+ 0.94% 95.44
HLA-DR+ 0.56% 95.20
% Stem-Trol Population
CD34+ (ML2 Tube) 9.26% 11.85
CD34+ (ML3 Tube) 7.00% 11.75
% MO7E Cell Line Population
CD117+ 9.23% 13.89
originalet.indd 5 10-06-18 15.23.25
LOWER LI MI T OF DETECTI ON:
A study was conducted in accordance with CLSI Approved Guidelines (4).
Results support a lower limit of detection of 0.3% when collecting 50,000 events.
Lambda - PE
K
a
p
p
a

-

F
I
T
C
10
0
10
0
10
1
10
2
10
3
10
1
10
2
10
3
0
1
0
2
3
10
0
10
1
10
2
10
3
CD19 - ECD
S
S

L
i
n
10
0
10
0
10
1
10
2
10
3
10
1
10
2
10
3
0
1
0
2
3
10
0
10
1
10
2
10
3
CD45 - PC7
S
S

L
i
n
10
0
10
0
10
1
10
2
10
3
10
1
10
2
10
3
0
1
0
2
3
10
0
10
1
10
2
10
3
CD45 - PC7
CD34 - ECD
CD7 - ECD
S
S

L
i
n
CD45 - PC7
S
S

L
i
n
C
D
1
1
7

-

P
C
5
.
5
C
D
2

-

F
I
T
C
Example of a precursor T-cell lymphoblastic leukemia /
lymphoma (T-ALL/LBL) bone marrow sample.
Figure 4 (above): CD45-PC7 vs. SS Histogram (Ungated).
Gate CD45
dim
SS
low
displays a large CD45
dim
popula-
tion.
Figure 5 (below): Tube TL1 displaying CD45
dim
/SS
low

gated events.
Example of an acute myeloid leukemia (AML) bone mar-
row sample.
Figure 6 (opposite): CD45-PC7 vs. SS Histogram (Unga-
ted). Gate CD45
dim
SS
low
displays a large CD45
dim
popu-
lation.
Figure 7 (above): Tube ML2 displaying CD45
dim
/SS
low

gated events.
originalet.indd 7 10-06-18 15.23.27
Example of a precursor T-cell lymphoblastic leukemia /
lymphoma (T-ALL/LBL) bone marrow sample.
Figure 4 (above): CD45-PC7 vs. SS Histogram (Ungated).
Gate CD45
dim
SS
low
displays a large CD45
dim
popula-
tion.
Figure 5 (below): Tube TL1 displaying CD45
dim
/SS
low

gated events.
Example of an acute myeloid leukemia (AML) bone mar-
row sample.
Figure 6 (opposite): CD45-PC7 vs. SS Histogram (Unga-
ted). Gate CD45
dim
SS
low
displays a large CD45
dim
popu-
lation.
Figure 7 (above): Tube ML2 displaying CD45
dim
/SS
low

gated events.
originalet.indd 7 10-06-18 15.23.27
SOL ASTR A T LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD2+ 130 85.24 72.63 95.35
CD5+ 130 78.65 61.42 88.13
CD7+ 130 81.53 69.74 91.04
CD56+ 130 14.38 3.97 32.34
CD3+ 130 77.82 62.42 88.43
CD3+CD4+ 130 49.52 17.61 70.19
CD3+CD8+ 130 26.68 12.01 52.40
SOL ASTRA MYELOMONOCYTI C LI NEAGE KI T /
NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD7+ 130 81.01 65.80 92.79
CD56+ 129 14.39 4.03 32.92
% Granulocytes
CD11b+ 130 99.66 97.58 100.00
CD13+ 129 99.41 92.36 100.00
CD15+ 130 99.68 97.55 99.99
CD16+ 130 95.48 84.59 99.68
% Monocytes
CD14+ 129 96.94 90.18 99.46
CD33+ 129 97.90 90.73 99.70
HLA-DR+ 130 98.40 88.69 99.95
PRECI SI ON:
The percent positive values were determined using IM-
MUNO-TROL (or, as necessary, IMMUNO-TROL with
Stem-Trol Control Cells, and IMMUNO-TROL with MO7E
cell line), run in duplicate, twice each day for up to 20 days
at 4 geographically diverse sites using the Solastra B, T
and Myelomonocytic Lineage Kit reagents.
SOL ASTR A B LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD19+ (BL1 Tube) 2.80% 14.20
CD19+ (BL2 Tube) 2.74% 14.34
CD20+ 3.56% 14.78
CD5+ 1.04% 71.86
% CD19 B Lymphocytes
CD19+Kappa+ 2.91% 57.42
CD19+Lambda+ 4.06% 40.23
% CD38 Monocytes
CD38+ 0.89% 94.45
% CD10+ Granulocytes
CD10+ 0.03% 99.96
SOL ASTR A T LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD2+ 0.67% 81.78
CD5+ 0.93% 72.73
CD7+ 0.73% 74.65
CD56+ 3.32% 13.76
CD3+ 0.76% 71.16
CD3+CD4+ 1.24% 45.48
CD3+CD8+ 1.94% 22.67
SOL ASTR A MYELOMONOCY TI C LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD7+ 1.59% 73.88
CD56+ 7.34% 14.16
% Granulocytes
CD11b+ 2.34% 97.26
CD13+ 0.43% 99.91
CD15+ 0.20% 99.76
CD16+ 0.56% 94.56
% Monocytes
CD14+ 0.91% 89.35
CD33+ 0.94% 95.44
HLA-DR+ 0.56% 95.20
% Stem-Trol Population
CD34+ (ML2 Tube) 9.26% 11.85
CD34+ (ML3 Tube) 7.00% 11.75
% MO7E Cell Line Population
CD117+ 9.23% 13.89
originalet.indd 5 10-06-18 15.23.25
SOL ASTR A T LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD2+ 130 85.24 72.63 95.35
CD5+ 130 78.65 61.42 88.13
CD7+ 130 81.53 69.74 91.04
CD56+ 130 14.38 3.97 32.34
CD3+ 130 77.82 62.42 88.43
CD3+CD4+ 130 49.52 17.61 70.19
CD3+CD8+ 130 26.68 12.01 52.40
SOL ASTRA MYELOMONOCYTI C LI NEAGE KI T /
NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD7+ 130 81.01 65.80 92.79
CD56+ 129 14.39 4.03 32.92
% Granulocytes
CD11b+ 130 99.66 97.58 100.00
CD13+ 129 99.41 92.36 100.00
CD15+ 130 99.68 97.55 99.99
CD16+ 130 95.48 84.59 99.68
% Monocytes
CD14+ 129 96.94 90.18 99.46
CD33+ 129 97.90 90.73 99.70
HLA-DR+ 130 98.40 88.69 99.95
PRECI SI ON:
The percent positive values were determined using IM-
MUNO-TROL (or, as necessary, IMMUNO-TROL with
Stem-Trol Control Cells, and IMMUNO-TROL with MO7E
cell line), run in duplicate, twice each day for up to 20 days
at 4 geographically diverse sites using the Solastra B, T
and Myelomonocytic Lineage Kit reagents.
SOL ASTR A B LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD19+ (BL1 Tube) 2.80% 14.20
CD19+ (BL2 Tube) 2.74% 14.34
CD20+ 3.56% 14.78
CD5+ 1.04% 71.86
% CD19 B Lymphocytes
CD19+Kappa+ 2.91% 57.42
CD19+Lambda+ 4.06% 40.23
% CD38 Monocytes
CD38+ 0.89% 94.45
% CD10+ Granulocytes
CD10+ 0.03% 99.96
SOL ASTR A T LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD2+ 0.67% 81.78
CD5+ 0.93% 72.73
CD7+ 0.73% 74.65
CD56+ 3.32% 13.76
CD3+ 0.76% 71.16
CD3+CD4+ 1.24% 45.48
CD3+CD8+ 1.94% 22.67
SOL ASTR A MYELOMONOCY TI C LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD7+ 1.59% 73.88
CD56+ 7.34% 14.16
% Granulocytes
CD11b+ 2.34% 97.26
CD13+ 0.43% 99.91
CD15+ 0.20% 99.76
CD16+ 0.56% 94.56
% Monocytes
CD14+ 0.91% 89.35
CD33+ 0.94% 95.44
HLA-DR+ 0.56% 95.20
% Stem-Trol Population
CD34+ (ML2 Tube) 9.26% 11.85
CD34+ (ML3 Tube) 7.00% 11.75
% MO7E Cell Line Population
CD117+ 9.23% 13.89
originalet.indd 5 10-06-18 15.23.25
REPRESENTATI VE RESULTS:
Example of a mature B-cell neoplasm bone marrow
aspirate sample.
Figure 1 (upper left): CD45-PC7 vs. SS Histogram
(Ungated). Gate Lymph displays a large CD45
bright

lymphocytic population.
Figure 2 (middle): Tube BL1 gated on Leukocytes
(Gate WBC).
Figure 3 (lower right): Tube BL1 gated on CD19+
lymphocytes (Gate CD19+ Ly).
Figure 4 (right): Tube BL2 gated on Lymphocytes
(Gate CD19+ Ly).
originalet.indd 6 10-06-18 15.23.26
REPRESENTATI VE RESULTS:
Example of a mature B-cell neoplasm bone marrow
aspirate sample.
Figure 1 (upper left): CD45-PC7 vs. SS Histogram
(Ungated). Gate Lymph displays a large CD45
bright

lymphocytic population.
Figure 2 (middle): Tube BL1 gated on Leukocytes
(Gate WBC).
Figure 3 (lower right): Tube BL1 gated on CD19+
lymphocytes (Gate CD19+ Ly).
Figure 4 (right): Tube BL2 gated on Lymphocytes
(Gate CD19+ Ly).
originalet.indd 6 10-06-18 15.23.26
SOL ASTR A T LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD2+ 130 85.24 72.63 95.35
CD5+ 130 78.65 61.42 88.13
CD7+ 130 81.53 69.74 91.04
CD56+ 130 14.38 3.97 32.34
CD3+ 130 77.82 62.42 88.43
CD3+CD4+ 130 49.52 17.61 70.19
CD3+CD8+ 130 26.68 12.01 52.40
SOL ASTRA MYELOMONOCYTI C LI NEAGE KI T /
NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD7+ 130 81.01 65.80 92.79
CD56+ 129 14.39 4.03 32.92
% Granulocytes
CD11b+ 130 99.66 97.58 100.00
CD13+ 129 99.41 92.36 100.00
CD15+ 130 99.68 97.55 99.99
CD16+ 130 95.48 84.59 99.68
% Monocytes
CD14+ 129 96.94 90.18 99.46
CD33+ 129 97.90 90.73 99.70
HLA-DR+ 130 98.40 88.69 99.95
PRECI SI ON:
The percent positive values were determined using IM-
MUNO-TROL (or, as necessary, IMMUNO-TROL with
Stem-Trol Control Cells, and IMMUNO-TROL with MO7E
cell line), run in duplicate, twice each day for up to 20 days
at 4 geographically diverse sites using the Solastra B, T
and Myelomonocytic Lineage Kit reagents.
SOL ASTR A B LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD19+ (BL1 Tube) 2.80% 14.20
CD19+ (BL2 Tube) 2.74% 14.34
CD20+ 3.56% 14.78
CD5+ 1.04% 71.86
% CD19 B Lymphocytes
CD19+Kappa+ 2.91% 57.42
CD19+Lambda+ 4.06% 40.23
% CD38 Monocytes
CD38+ 0.89% 94.45
% CD10+ Granulocytes
CD10+ 0.03% 99.96
SOL ASTR A T LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD2+ 0.67% 81.78
CD5+ 0.93% 72.73
CD7+ 0.73% 74.65
CD56+ 3.32% 13.76
CD3+ 0.76% 71.16
CD3+CD4+ 1.24% 45.48
CD3+CD8+ 1.94% 22.67
SOL ASTR A MYELOMONOCY TI C LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD7+ 1.59% 73.88
CD56+ 7.34% 14.16
% Granulocytes
CD11b+ 2.34% 97.26
CD13+ 0.43% 99.91
CD15+ 0.20% 99.76
CD16+ 0.56% 94.56
% Monocytes
CD14+ 0.91% 89.35
CD33+ 0.94% 95.44
HLA-DR+ 0.56% 95.20
% Stem-Trol Population
CD34+ (ML2 Tube) 9.26% 11.85
CD34+ (ML3 Tube) 7.00% 11.75
% MO7E Cell Line Population
CD117+ 9.23% 13.89
originalet.indd 5 10-06-18 15.23.25
SOL ASTR A T LI NE AGE KI T / NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD2+ 130 85.24 72.63 95.35
CD5+ 130 78.65 61.42 88.13
CD7+ 130 81.53 69.74 91.04
CD56+ 130 14.38 3.97 32.34
CD3+ 130 77.82 62.42 88.43
CD3+CD4+ 130 49.52 17.61 70.19
CD3+CD8+ 130 26.68 12.01 52.40
SOL ASTRA MYELOMONOCYTI C LI NEAGE KI T /
NORMAL WHOLE BLOOD:
MEASUREMENT N MEAN INTERVAL
LOWER UPPER
% Lymphocytes
CD7+ 130 81.01 65.80 92.79
CD56+ 129 14.39 4.03 32.92
% Granulocytes
CD11b+ 130 99.66 97.58 100.00
CD13+ 129 99.41 92.36 100.00
CD15+ 130 99.68 97.55 99.99
CD16+ 130 95.48 84.59 99.68
% Monocytes
CD14+ 129 96.94 90.18 99.46
CD33+ 129 97.90 90.73 99.70
HLA-DR+ 130 98.40 88.69 99.95
PRECI SI ON:
The percent positive values were determined using IM-
MUNO-TROL (or, as necessary, IMMUNO-TROL with
Stem-Trol Control Cells, and IMMUNO-TROL with MO7E
cell line), run in duplicate, twice each day for up to 20 days
at 4 geographically diverse sites using the Solastra B, T
and Myelomonocytic Lineage Kit reagents.
SOL ASTR A B LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD19+ (BL1 Tube) 2.80% 14.20
CD19+ (BL2 Tube) 2.74% 14.34
CD20+ 3.56% 14.78
CD5+ 1.04% 71.86
% CD19 B Lymphocytes
CD19+Kappa+ 2.91% 57.42
CD19+Lambda+ 4.06% 40.23
% CD38 Monocytes
CD38+ 0.89% 94.45
% CD10+ Granulocytes
CD10+ 0.03% 99.96
SOL ASTR A T LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD2+ 0.67% 81.78
CD5+ 0.93% 72.73
CD7+ 0.73% 74.65
CD56+ 3.32% 13.76
CD3+ 0.76% 71.16
CD3+CD4+ 1.24% 45.48
CD3+CD8+ 1.94% 22.67
SOL ASTR A MYELOMONOCY TI C LI NE AGE KI T
MEASUREMENT REPEATABILITY
(CV %)
MEAN
% Lymphocytes
CD7+ 1.59% 73.88
CD56+ 7.34% 14.16
% Granulocytes
CD11b+ 2.34% 97.26
CD13+ 0.43% 99.91
CD15+ 0.20% 99.76
CD16+ 0.56% 94.56
% Monocytes
CD14+ 0.91% 89.35
CD33+ 0.94% 95.44
HLA-DR+ 0.56% 95.20
% Stem-Trol Population
CD34+ (ML2 Tube) 9.26% 11.85
CD34+ (ML3 Tube) 7.00% 11.75
% MO7E Cell Line Population
CD117+ 9.23% 13.89
originalet.indd 5 10-06-18 15.23.25
RE AGENTS AVAI L ABI LI T Y ( CE- I VD) :
Solastra B Lineage Kit: PN A66286
Solastra T Lineage Kit: PN A66287
Solastra Myelomonocytic Lineage Kit: PN A66288
Solastra Negative Control Tube: PN A66256
Solastra QuickCOMP 5 Kit: PN A83571
TERM DEFI NI TI ONS:
ACD Acid Citrate Dextrose
CLSI Clinical and Laboratory
Standards Institute
ECD Energy Coupled Dye (PE-Texas Red-x)
EDTA Ethylenediaminetetraacetic acid
FC 500 Beckman Coulter Cytomics FC 500 Flow
Cytometer series
FITC Fluorescein isothiocyanate
HIFCS Heat-Inactivated Fetal Calf Serum
HIMS Heat-Inactivated Mouse Serum
N/A Not Applicable
PC5.5 PE-Cyanine 5.5
PC7 PE-Cyanine 7
PE R-Phycoerythrin
SS Side scatter
BI BLI OGR APHY
1. Wood BL, Arroz M, Barnett D, DiGiuseppe J, Greig B,
Kussick SJ, Oldaker T, Shenkin M, Stone E and Wal-
lace P, 2006 Bethesda International Consensus Re-
commendations on the Immunophenotypic Analysis of
Hematolymphoid Neoplasia by Flow Cytometry: Optimal
reagents and reporting for the fow cytometric diagnosis
of hematopoietic neoplasia. Cytometry Part B (Clinical
Cytometry), 72B:S14-22 (2007).
2. Davis BH, Holden JT, Bn MC, Borowitz MJ, Braylan
RC, Cornfeld D, Gorczyca W, Lee R, Maiese R, Orfao
A, Wells D, Wood BL and Stetler-Stevenson M, 2006
Bethesda International Consensus Recommendations
on the Flow Cytometric Immunophenotypic Analysis
of Hematolymphoid Neoplasia: Medical Indications.
Cytometry Part B (Clinical Cytometry) 72B:S5S13
(2007).
3. Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA,
Stein H, Thiele J and Vardiman JW (Eds.): WHO Clas-
sifcation of Tumours of Hmatopoietic and Lymphoid
Tissues, 4th Edition, IARC Press, Lyon (2008).
4. Tholen DW, Protocols for Determination of Limits of
Detection and Limits of Quantitation, CLSI EP17A
Approved Guideline, First Edition (2004).
: CE-marking as per the requirements of the European
In Vitro Diagnostics Directive (98/79/EC), following
declaration of conformity to the European Regulatory
Bodies
: Not available in the U.S., pending submission and clea-
rance by the U.S. FDA.
: Product in development.
Beckman Coulter and the stylized logo are registered tra-
demarks of Beckman Coulter, Inc. Flow-Check, Flow-Set,
IMMUNO-TROL, IOTest, Solastra, Stem-Trol and VersaLyse
are trademarks of Beckman Coulter, Inc. Texas-Red is a
trademark of Molecular Probes, Inc.
Australia, Gladesville (61) 2 9844 6000 Canada, Mississauga (1) 905 819 1234 China, Beijing (86) 10 6515 6028 Czech Republic, Prague (420) 272 01
73 32 Eastern Europe, Middle East, North Africa, South West Asia: Switzerland, Nyon (41) 22 365 3707 France, Villepinte (33) 1 49 90 90 00 Germany,
Krefeld (49) 2151 33 35 Hong Kong (852) 2814 7431 India, Mumbai (91) 22 3080 5000 Italy, Cassina de Pecchi, Milan (39) 02 953921 Japan, Tokyo (81)
3 5530 8500 Korea, Seoul (82) 2 404 2146 Latin America (1) (305) 380 4709 Mexico, Mexico City (001) 52 55 9183 2800 Netherlands, Woerden (31) 348
462462 Puerto Rico (787) 747 3335 Singapore (65) 6339 3633 South Africa/Sub-Saharan Africa, Johannesburg (27) 11 564 3203 Spain, Madrid (34) 91
3836080 Sweden, Bromma (46) 8 564 85 900 Switzerland, Nyon (41) 0800 850 810 Taiwan, Taipei (886) 2 2378 3456 Turkey, Istanbul (90) 216 570 17 17
UK, High Wycombe (44) 01494 441181 USA, Brea, CA (1) 800 352 3433, (1) 714 993 5321
B2010-05-25/Solastra www.beckmancoulter.com 2010 Beckman Coulter, Inc. PRINTED IN U.S.A.
originalet.indd 8 10-06-18 15.23.29
10
0
10
0
10
1
10
2
10
3
10
1
10
2
10
3
0
1
0
2
3
10
0
10
1
10
2
10
3
10
0
10
0
10
1
10
2
10
3
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3
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10
1
10
2
10
3
0
1
0
2
3
10
0
10
1
10
2
10
3
RE AGENTS AVAI L ABI LI T Y ( CE- I VD)

Solastra B Lineage Kit: PN A66286

, 25 tests
- Solastra B Lineage Vial BL1: Kappa-FITC / Lambda-PE / CD19-ECD / CD5-PC5.5 / CD45-PC7
- Solastra B Lineage Vial BL2: CD20-FITC / CD10-PE / CD19-ECD / CD38-PC5.5 / CD45-PC7
Solastra T Lineage Kit: PN A66287

, 25 tests
- Solastra T Lineage Vial TL1: CD2-FITC / CD56-PE / CD7-ECD / CD5-PC5.5 / CD45-PC7
- Solastra T Lineage Vial TL2: CD8-FITC / CD4-PE / / CD3-PC5.5 / CD45-PC7
Solastra Myelomonocytic Lineage Kit: PN A66288

, 25 tests
- Solastra M. Lineage Vial ML1: CD15-FITC / CD11b-PE / CD16-ECD / CD14-PC5.5 / CD45-PC7
- Solastra M. Lineage Vial ML2: HLADR-FITC / CD56-PE / CD34-ECD / CD117-PC5.5 / CD45-PC7
- Solastra M. Lineage Vial ML3: CD7-FITC / CD13-PE / CD34-ECD / CD33-PC5.5 / CD45-PC7
Solastra QuickCOMP 5 Kit: PN A83571

, 25 tests
- Solastra QuickCOMP 5 Vial 1: CD45-FITC
- Solastra QuickCOMP 5 Vial 2: CD45-PE
- Solastra QuickCOMP 5 Vial 3: CD45-ECD
- Solastra QuickCOMP 5 Vial 4: CD45-PC5.5
- Solastra QuickCOMP 5 Vial 5: CD45-PC7
Beckman Coulter and the stylized logo are registered trademarks of Beckman Coulter,
Inc. Flow-Check, Flow-Set, IMMUNO-TROL, IOTest, Solastra, Stem-Trol and VersaLyse
are trademarks of Beckman Coulter, Inc. Texas-Red is a trademark of Molecular
Probes, Inc.
BI BLI OGR APHY
1. Wood BL, Arroz M, Barnett D, DiGiuseppe J, Greig B,
Kussick SJ, Oldaker T, Shenkin M, Stone E and Wallace P,
2006 Bethesda International Consensus Recommenda-
tions on the Immunophenotypic Analysis of Hematolymp-
hoid Neoplasia by Flow Cytometry: Optimal reagents and
reporting for the fow cytometric diagnosis of hematopoietic
neoplasia. Cytometry Part B (Clinical Cytometry), 72B:S14-
22 (2007).
2. Davis BH, Holden JT, Bn MC, Borowitz MJ, Braylan RC,
Cornfeld D, Gorczyca W, Lee R, Maiese R, Orfao A, Wells
D, Wood BL and Stetler-Stevenson M, 2006 Bethesda
International Consensus Recommendations on the Flow
Cytometric Immunophenotypic Analysis of Hematolymp-
hoid Neoplasia: Medical Indications. Cytometry Part B
(Clinical Cytometry) 72B:S5S13 (2007).
3. Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA,
Stein H, Thiele J and Vardiman JW (Eds.): WHO Classifca-
tion of Tumours of Hmatopoietic and Lymphoid Tissues,
4th Edition, IARC Press, Lyon (2008).
4. Tholen DW, Protocols for Determination of Limits of De-
tection and Limits of Quantitation, CLSI EP17A Approved
Guideline, First Edition (2004).
: CE-marking as per the requirements of the European In
Vitro Diagnostics Directive (98/79/EC), following declara-
tion of conformity to the European Regulatory Bodies
: Not available in the U.S., pending submission and clea-
rance by the U.S. FDA.
: Product in development.
For information about Beckman Coulter products for ow cytometry and immunophenotyping, please consult: www.coulterow.com
Australia, Gladesville (61) 2 9844 6000 Austria, Vienna (43) 662 857220 72 Canada,
Mississauga (1) 905 819 1234 China, Shanghai (86) 21 3865 1000 Croatia, Zagreb (38)
51 489 9003 Czech Republic, Prague (420) 272 017 999 Eastern Europe, Middle East,
North Africa, South West Asia: Switzerland, Nyon (41) 22 365 3707 France, Villepinte
(33) 1 49 90 90 00 Germany, Krefeld (49) 2151 33 35 Hong Kong (852) 2814 7431 Hungary,
Budapest (361) 25 09 330 India, Mumbai (91) 22 3080 5000 Italy, Cassina de Pecchi,
Milan (39) 02 953921 Japan, Tokyo (81) 3 5530 8500 Korea, Seoul (82) 2 404 2146 Latin
America (1) (305) 380 4709 Malaysia, Kuala Lumpur (60) 3 5621 4793 Mexico, Mexico
City (001) 52 55 9183 2800 Netherlands, Woerden (31) 348 462462 Poland, Warszawa
(48) 22 366 0180 Puerto Rico (787) 747 3335 Russia, Moscow (7) 495 9846730 Singapore
(65) 6339 3633 South Africa/Sub-Saharan Africa, Johannesburg (27) 11 564 3203 Spain,
Madrid (34) 91 3836080 Sweden, Bromma (46) 8 564 85 900 Switzerland, Nyon (41) 0800
850 810 Taiwan, Taipei (886) 2 2730 2500 Turkey, Istanbul (90) 216 570 17 17 UK, High
Wycombe (44) 01494 441181 USA, Brea, CA (1) 800 352 3433, (1) 714 993 5321
BR-14088A www.beckmancoulter.com 2010 Beckman Coulter, Inc.