Old and New Ways To RNA

page 52 Lab Times 2-2012 Products
U
ntil the middle of the last century,
RNA was isolated similar to DNA
by time-consuming and laborious
CsCl-centrifugation. Obviously too time-
consuming for K. S. Kirby who worked on
cancerogenic effects of tannin in the 1950s
at the Institute of Cancer Research in Lon-
don. Inspired by biochemists using phenol
to extract proteins from crude extracts, Kir-
by tried applying a phenol-water mixture to
extract RNA. Kirbys method has since been
slightly modified; however, the basic princi-
ples of phenol extraction, which is still quite
popular, remain the same.
The crude extract containing RNA and
other cellular components is treated with a
phenol-water mixture that adapts an acidic
pH of 4.8. Proteins are solved in the organic
phenol phase, while RNA molecules stay in
the aqueous phase and DNA accumulates in
the phenol-water interphase. Traces of phe-
nol in the aqueous phase are subsequently
removed by one or two further chloroform
extraction steps. The upper water phase
containing the RNA is then removed from
the phenol phase with a pipette.
Since phenol and water have similar
densities, and phases may not clearly sep-
arate from each other or may even invert,
Kirby later refined his extraction method.
Instead of phenol, he used a phenol-chloro-
form mixture that gravitates to the bottom
of the reaction vessel due to chloroforms
high density, with the water phase always
on top.
Single extraction step
Pietro Chomczynski and Nicoletta Sac-
chi further improved phenol-chloroform ex-
traction in the late eighties by adding guan-
idinium thiocyanate (GTC) to the phenol-
chloroform mixture and lowering its pH to
4 with sodium acetate buffer. Chomczyn-
skis acid phenol method integrates all ex-
traction steps into a single one, followed by
a simple alcohol precipitation of the extract-
ed RNA. Due to its high yields, reproducibil-
ity, throughput and time saving, acid phe-
nol extraction has become the gold stand-
ard for numerous home-made extraction
mixtures and individually-designed RNA
extraction protocols. Chomcynski was clev-
er enough to patent his GTC-Acidic phenol
solution, which is also found in commercial
RNA extraction reagents such as Trizol and
Tri as well as in some RNA extraction kits.
However, if you have ever extracted
RNA with acidic phenol, or even worse,
melted phenol, to produce a freshly pre-
pared water-saturated phenol solution,
you will certainly agree that phenol is not
the most pleasant substance to work with.
The same holds true for chloroform and the
strange smelling isoamylalcohol. Hence,
RNA guys were happy when, in the 1990s,
small spin columns packed with silica gel
membranes became available and revolu-
tionised RNA extraction.
Silica spin columns are, essentially,
small, solid-phase extraction columns con-
taining silica as solid-phase material that
specifically binds RNA under defined salt
and buffer conditions. Extraction of RNA
with spin columns usually follows four sim-
ple, idiot-proof steps common to all solid
phase extractions: column-conditioning,
sample application, washing and elution.
To simplify things even more, the first step
may be omitted in many commercial kits
based on spin columns, starting directly
with the sample application.
Bead-based extraction
However, adsorbent-based RNA extrac-
tion methods are not restricted to silica spin
columns. More and more kit suppliers rely
on tiny beads made of RNA-adsorbent ma-
terials, such as polystyrene-latex, glass fi-
bres, cellulose or silica, to extract RNA.
The basic steps of bead-based RNA isola-
tion are as easy as extracting RNA with sili-
ca spin columns. The sample is first lysed in
buffer containing guanidinium thiocyanate
and exposed to the beads in small reaction
tubes. Subsequently, RNA-bound beads are
repeatedly washed and centrifuged to re-
move any contaminants. After washing, a
chaotropic salt-free buffer is added to re-
lease the RNA. Beads devoid of RNA are
then sedimented by a further centrifuga-
tion step and RNA is finally recovered from
the supernatant.
However, even the centrifugation steps
are dispensable if magnetic beads com-
posed of an iron oxide core, coated with
silica or other RNA-adsorbing materials, are
used. Instead of a centrifuge, a magnet that
may be embedded, for example, in a tube
stand, draws the beads to the bottom of the
reaction tubes during washing and elution.
Since all required washing and elution
steps of spin column and magnetic bead
extraction may easily be performed by the
nimble arms of a liquid handler, most au-
tomatic RNA extractors either work with
modified silica spin columns or bead-based
extraction kits. However, the time-consum-
ing washing steps that lead to high con-
sumptions of expensive RNA extractor tips
and wash solutions are still a major short-
coming of these techniques.
Immiscible filtration
A very interesting concept that avoids
repeated washing steps during RNA extrac-
tion with magnetic beads has recently been
presented by the group of David Beebe from
the University of Wisconsin (Berry et al.,
Lab Chip, 2011, 11, 1747). The group re-
placed all washing steps by a single traverse
of the bead-bound RNA through an immis-
cible fluid barrier in a process they call Im-
miscible Filtration Assisted by Surface Ten-
sion (IFAST).
This new technique may sound compli-
cated in the first place, however, it is plain
simple. To apply IFAST, Berry et al. con-
structed an IFAST-plate consisting of three
wells (input-, immiscible phase- and output
well) connected in series by micro channels.
The input well is loaded with cell suspen-
sion, lysis buffer and magnetic beads, while
the output well contains the elution buffer.
Both wells are separated by the immiscible
phase well in the centre, filled with a spe-
cial oil to prevent mixing of the liquids. Dur-
ing the washing step, a permanent magnet
draws the magnetic beads and the bound
RNA through the immiscible oil-phase into
the elution buffer of the output well. Since
hydrophilic contaminants cannot traverse
the oil phase, they are captured in the input
well. The whole IFAST-extraction procedure
takes only a few minutes, which is consid-
erably faster than RNA extraction with con-
ventional kits. Harald Zhringer
Product survey: RNA extraction
Old and New Ways to RNA
RNA extraction is the first step in a multitude of analytical molecular biological techniques that require
high-quality RNA, free of RNAase, enzyme inhibitors, proteins and DNA.
Lab Times 2-2012 page 53 Products
Company/Distributor
Table 1: Devices and/or Kits for Automatic RNA Extraction
Price [EUR] Name of Device/Kit Extraction Technology Suitable samples / RNA type
Agilent Technologies
www.genomics.agilent.com
Amsbio
Abingdon, UK
www.amsbio.com
Contact: info@amsbio.com
Phone: +44 1235 828200
Analytik Jena
Life Science
Jena, Germany
www.bio.analytik-jena.de
Contact: lifescience@
analytik-jena.de
Phone +49 36 4177-9400
Beckman Coulter
Krefeld, Germany
www.beckmancoulter.com
Contact: Christoph Krll
Phone +49 2151 333 723
ckruell@beckman.com
Chemicell
www.chemicell.com
Contact: Cengiz ztrk
Phone +49 30-2141481
cengiz@chemicell.com
Axygen Bioscience
Corning Life Sciences
Amsterdam, The
Netherlands
www.axygenbio.com
Contact: Joanne Woodward
Phone +44 7788 645292
WoodwardJ2@corning.com
Eppendorf
www.eppendorf.com
Contact: automation-
support@eppendorf.de
Phone +49-4053801-0
GE Healthcare
www.gelifesciences.com/
sampleprep
Contact: Sally Woods
Please contact your local
service department

2 plates / Sets of 96 samples of 10 to 5 10
5
cells
each
10 plates Sets of 96 samples of 10 to 5 10
5
cells
each
Isolation of total RNA from FFPE tissue samples

Total RNA from tissue samples, cells (eucaryotic and
procaryotic) and plant samples
Total RNA from tissue samples, cells (eucaryotic and
procaryotic) and plant samples
Total RNA from a wide variety of tissue samples

Total plant RNA

Isolating of total RNA from eukaryotic and prokaryotic
cells (gram+ and gram bacteria)
Cultured cells, total RNA

Tissues, total RNA
Formalin fixed, paraffin embedded sections;
total RNA or total nucleic acid

PAXgene stabilized blood samples, total RNA

Isolation of total-RNA/DNA from 10
9
bacteria cells

Maximum weight of 10 mg of any formalin-fixed,
paraffin embedded tissue sample

Suitable for many molecular biology applications, where
automated platforms are not required
Suitable for many molecular biology applications, where
automated platforms are not in place
Total RNA, mRNA, viral RNA, using pretested PlugnPrep
methods for various kit suppliers

Cultured cells, tissue, bacteria or yeast

Processing of tissues with illustra RNAspin Mini 96

666.-
2,780.-
745.-

14,900.-
35,000.-
138.-
670.-
2,670.-
138.-
670.-
2,670.-
138.-
670.-
2,670.-
On request

On request
On request

On request

185.-
725.-

404.26
1,341.68

691.88
953.50

Please inquire

1,170.-

212.-

High Throughput RNA purification kit in a
96 well format
See above
FFPE tissue RNA isolation kit for the
Fujifilm QuickGene-Mini80 instrument

Nucleic acids are adsorbed onto magnetic
or paramagnetic particles
Fully automated nucleic acid extraction based
on proven magnetic particle separation
Automated extraction of total RNA

Optimised for automatically extracting

Pre-filter for fast, selective removal of DNA
(no DNase I digestion) greatly reduces
time required
Extraction of total RNA with Solid Phase
Reversible Immobilisation (SPRI)
paramagnetic beads from 1-96 samples
in 96 plate format on Biomek FXp or
NXp liquid handlers
Total RNA extraction with SPRI on Biomek
FXp or NXp span8 liquid handlers
RNA Extraction with Solid Phase
Reversible Immobilisation (SPRI)
paramagnetic bead-based technology
utilizing the Beckman Coulter Biomek
NXP or FXP Span-8 workstation
RNA extraction with Solid Phase Reversible
Immobilisation (SPRI) paramagnetic bead-
based technology on Beckman Coulter
Biomek FXp or NXp liquid handler
Magnetic RNA/DNA purification kit com-
patible for KingFisher 96 or BioSprint 96
workstation

Magnetic beads-based technology

A hand held Magnetic Bead Separation
Device (MSD) for up to 12 tubes
A hand held Magnetic Bead Separation
Device (MSD) for one 96 well microplate
(standard or deep well)
Small benchtop automated liquid
handling system /
Apply magnetic bead separation (TMX
version) or vacuum method (VAC) for RNA
purification methods
Simultaneous processing of 96 samples
via either centrifugation or vacuum-based
protocols
Accessory product that facilitates the
use of tissues as input material for
96 well total RNA extraction with the
RNAspin 96 Kit
Absolutely RNA 96 Micro-
prep kit, 2 plates
Absolutely RNA 96 Micro-
prep kit, 10 plates
ExpressArt QuickGene
FFPE RNAready kit

InnuPure C16
InnuPure C96
innuPREP Tissue RNA
Kit - IP
innuPREP Plant RNA
Kit - IP
innuPREP Cell RNA Kit - IP

RNAdvance Cell v2

RNAdvance Tissue
Formapure

RNAdvance Blood

geneMAG-RNA/DNA 96
geneMAG-RNA/DNA 480

AxyPrep MAG / FFPE /
DNA RNA- miRNA Kit
Large: 384 preps
Medium: 96 preps
IMAG-12T Device
IMAG-96P Device

epMotion 5075 TMX
epMotion 5075 VAC

illustra RNAspin 96 Kit

illustra RNAspin 96 Filter
Plate

Company/Distributor
Hamilton Bonaduz
Bonaduz, Switzerland
Contact: www.
hamiltoncompany.com
Life Technologies
www.lifetechnologies.com
Darmstadt, Germany
Contact: Anke Werse
debra.nickson@
lifetech.com
LTF Labortechnik
Wasserburg, Germany
www.labortechnik.com
Contact: Rudolf Walser
Phone +49 8382-98520
info@labortechnik.com
Macherey-Nagel
Dueren, Germany
www.mn-net.com
Contact: Michael Bumann
Phone +49 2421969270
tech-bio@mn-net.com
Miltenyi Biotec
Bergisch Gladbach,
Germany
www.miltenyibiotec.com
Contact:
macs@miltenyibiotec.de
PerkinElmer
Chemagen Technologie
Baesweiler, Germany
www.chemagen.com
Contact: Andreas Knzel
Phone +49 2401-805500
andreas.kuenzel@
perkinelmer.com
Contact: Christian Henze
Christian.henze@
perkinelmer.com
Contact: Jrgen Oster
Juergen.oster@
perkinelmer.com
Total RNA from tissue and cells
(4 and 8 channel, manual or autoload load)

Total RNA from blood in PAXgene tubes

Viral, blood, plant, tissue, cells
See above

Viral samples, cultured cells, blood, and plant samples

Total RNA from various samples /
Virus-RNA from sputum, stool, swabs, blood, tissue,
serum, plasma, cell pellets, urine
RNA from plant and human samples, virus, sputum,
stool, swabs, blood, tissue, serum, plasma,
cell pellets, urine
Tissue, cells /
Total RNA > 200 nt
Tissue, cells /
Total RNA > 200 nt
Tissue, cells /
Total RNA > 200 nt
Whole blood /
Total RNA > 200 nt
Whole blood /
Total RNA > 200 nt
mRNA from cells, tissue samples, total RNA,
or whole blood
or whole blood
or whole blood (12 x 8 or 4 x 96 reactions)
or whole blood (12 x 8 or 4 x 96 reactions)
Blood, stabilized blood, PAXgene blood,
serum/plasma, urine, stool suspension, swabs, cells,
tissue

Blood, serum/plasma, urine, stool suspension, swabs,
cells, tissue
2 ml blood / m/RNA
2.5 ml PAXgene blood
Animal or plant tissue, cells /
Scalable for extraction of at least 100 g mRNA/Kit
Blood / mRNA

69,000.-
85,000.-
78,000.-
94,000.-
149,900.-

14,430.-
39,270.-

28,930.-

From 8,900.-

From 21,900.-

On request
On request
On request
On request
On request
On request
On request
On request
On request
On request

On request

On request
On request
On request

On request

Total RNA extraction with 96 Nucleospin
kit from M&N

Total RNA extraction out of PAXgene
blood tubes either 2 x 24 or 2 x 12 sample
per run with the chemistry from
Chemagen
Processing of magnetic magnetic beads
Processing of magnetic beads

Processes up to 12 samples per run
plus a control. Integrated Charge-
Switch technology for inhibitor-free
purification
Magnetic beads

Membrane columns

Silica-membrane technology
Magnetic-bead technology
The instrument works in combination with
a wide range of MultiMACS Kits
See above / Heating functionality to heat
up samples on-column at 37C or 42C
Superparamagnetic Oligo(dT) MicroBeads
and MACS Columns
Superparamagnetic Oligo(dT) MicroBeads
Automated nucleic acid isolation;
Magnetic Bead based technology

Compact benchtop system;
Magnetic bead based technology
Magnetic bead based technology;
kit for chemagic MSM I
kit for chemagic MSM I
kit for manual use with chemagic
Stands
several kits for chemagic MSM I

GenomicsStarlet
4 channel, manual (ML)
or autoload load (AL)
8 channel manual or
autoload
chemagicSTAR

MagMAX Express Magnetic
Particle Processor
MagMAX Express-96 Deep
Well Magnetic Particle
Processor
iPrep Purification
Instrument

Arrow / Automated RNA
Extraction
LabTurbo

NucleoSpin 8 RNA
NucleoSpin 96 RNA
NucleoMag RNA
NucleoSpin 8 RNA Blood
NucleoSpin 96 RNA Blood
MultiMACS M96 Separator
MultiMACS M96thermo
Separator
MultiMACS mRNA
Isolation Kit
MultiMACS cDNA
Synthesis Kit
chemagic Magnetic
Separation Module I

chemagic Prepito

chemagic mRNA/gDNA Kit
special
chemagic Total RNA Kits
chemagic mRNA Direct Kit

chemagic DNA Blood Kits
special

Company/Distributor
Qiagen
Hilden, Germany
www.qiagen.com
Contact: Salim Essakali
Phone +49 21032911343
salim.essakali@qiagen.com
Roche Diagnostics
Mannheim, Germany
www.roche-applied-
science.com
Contact: Christian Knop
christian.knop@roche.com
Stratec Molecular
Berlin, Germany
www.invitek.de
Contact: Phone
+49 30 9489 2901/2910
info.berlin@stratec.com
Blood (human/animal), tissues, FFPE-tissues,
lipid tissues, microarray-tissues, cells, bacteria and
plant material
Total RNA from cells and easy-to-lyse tissues
(e.g., kidney, liver, spleen)
Blood, tissue, and cells using Qiagen kits

Total RNA from cells and easy-to-lyse tissues
(e.g., kidney, liver, spleen)
Total RNA from all types of tissues
Total RNA and genomic DNA from a variety of sample
types (whole blood, buccal swab, etc.)
Total RNA from animal cells, tissues, or FFPE tissues /
Total RNA including miRNA and other small RNAs after
proteinase K treatment
Total RNA, including miRNA, from blood
Total RNA from whole blood, culture cells, fresh-frozen
tissue, RNAlater stabilized tissue, FFPE tissue, WBC
sediment of PAXgene tubes;
viral RNA from whole blood, serum, etc.
Total RNA from whole blood, blood cells, culture cells,
fresh-frozen tissue, FFPE tissue;
viral RNA from whole blood, serum, plasma
Total RNA from whole blood, blood cells, culture cells,
fresh-frozen tissue, RNAlater stabilized tissue;
viral RNA from serum and plasma
EDTA- and citrate stabilized blood, serum, plasma,
urine, swabs, saliva, etc.
Viral RNA from 200 l blood, serum, plasma, rinse
liquid from swabs, cell-free body fluids
Viral RNA from 200 l serum or plasma, cell-free body
fluids, rinse liquid from swabs, cell culture supernatants
and 50mg stool sample
Viral RNA from up to 200 l serum, plasma, cell-free
body fluids, etc.
Viral RNA from up to 200 l serum, plasma, cell-free
body fluids, etc.
Viral RNA (and DNA) from up to 200 l serum, plasma,
cell-free body fluids, cell culture supernatants, etc.
Viral RNA (and DNA) from up to 200 l serum, plasma,
cell-free body fluids, cell culture supernatant, etc.
Viral RNA from up to 200 l of plasma, serum, cell-free
body fluids etc.
Viral RNA from up to 200 l of plasma, serum, cell-free
body fluids of human origin, etc.
Total RNA from up to 5 x 10
6
cells, up to 1.0 ml blood,
up to 20 mg tissue sample
Total RNA from up to 5 x 10
6
cells, up to 1.5 ml blood,
10-40 mg tissue

Please inquire

Please inquire

Please inquire

3,503.-
4,513.-
3,935.-
Please inquire

Please inquire
Please contact
local Roche
representative
Please contact
local Roche
representative
Please contact
local Roche
representative
27,500.-
(German price)
630.-
605.-

90.-
324.-
598.-
237.50
828.-
73.50
294.50
529.50
261.-
1,300.50
774.-
4,567.-
415.-
735.-
4,337.-
110.-
400.-
310.-
1,122.-

Automation of silica-membrane based
extraction of nucleic acids from
1-12 samples at a time
RNA purification based on silica
technology dedicated for use on the
QIAcube
Medium- to high-throughput silica-
membranebased extraction of nucleic
acids in 96 well format
Silica technology in a 96 well format
Silica technology + QIAzol in a 96 well
format
Silica technology in a 96 well format
Combines silica-based technology with
magnetic particles
Magnetic-particle technology
Magnetic glass particle technology
(silica-based purification)



Fully automated system for DNA/RNA
extraction with magnetic bead based
Magnetic bead based, for use on the
InviGenius
InviGenius
KingFisher mL (Thermo Scientific)
KingFisher 96/Flex (Thermo Scientific)
KingFisher mL (Thermo Scientific)
KingFisher 96/Flex (Thermo Scientific)
Membrane adsorption, filter plates for use
on X-tractor Gene, 96 well format
Membrane adsorption, filter plates, for use
on epMotion 5075 VAC (Eppendorf),
96 well format
Magnetic bead based, for use on the King-
Fisher mL (Thermo Scientific)
Magnetic bead based, for use on the King-
Fisher 96/Flex (Thermo Scientific)

QIAcube

RNeasy Mini QIAcube Kit

BioRobot Universal

RNeasy 96 BioRobot
8000 Kit
RNeasy 96 Universal
Tissue 8000 Kit
QIAamp One-for- All
Nucleic Acid Kit
QIAsymphony RNA Kit

QIAsymphony PAXgene
Blood RNA Kit
MagNA Pure 96 System

MagNA Pure LC 2.0
System

MagNA Pure Compact
System

InviGenius

InviMag Universal Kit/IG
(8 x 12 preps)
InviMag Virus RNA Mini
Kit/IG (8 x 12 preps)
InviMag Virus RNA Kit/
KFmL
(15, 75, 150 preps)
InviMag Virus RNA Kit/
KF96
(1 x 96, 5 x 96 preps)
InviMag Virus DNA/RNA
Mini Kit/KFmL
(15, 75, 150 preps)
InviMag Virus DNA/RNA
Mini Kit/KF96
(1 x 96, 5 96 preps)
Invisorb Virus RNA HTS
96 Kit/X
(4 x 96, 24 x 96 preps)
Invisorb Virus RNA HTS 96
Kit/ep (2 x 96, 4 x 96,
24 x 96 preps)
InviMag Universal RNA
Mini Kit/KFmL
(15, 75 preps)
InviMag Universal RNA
Mini Kit/KF96
(1 x 96, 5 x 96 preps)

Company/Distributor
Table 2: Kits for Manual RNA Extraction
Price [EUR] Name of Product Extraction Technology Suitable samples / RNA type
Active Motif
La Hulpe, Belgium
www.activemotif.com
Contact: Claire LaCroix
Phone +32 2 653 0001
eurotech@activemotif.com
5 PRIME
Hamburg, Germany
www.5PRIME.com
Contact:
Phone +49 40 3197927-0
info@5prime.com
Agilent Technologies
www.genomics.agilent.com
Amsbio
Abingdon, UK
www.amsbio.com
Contact: info@amsbio.com
Phone: +44 1235 828200
0,5-2 x 10
8
cells or 0.4-1.0 g tissue /
100-200 g mRNA

0,5-1 x 10
7
cells or 50-200 mg tissue /
10-20 g mRNA
Up to 500 g total RNA /
10-15 g mRNA
Whole blood and buffy coat
Cultured cells
Variety of tissue types
Fibrous tissue (heart, skeletal muscle or skin)
5,000 to 5 million cells

Variety of tissue types
Small samples of cultured cells (110
4
cells) or cells
harvested by laser capture microdissection (LCM)
Samples containing a very small number of cells
(1 cell to 5 x 10
5
cells)
Purification of high-quality total RNA from small
samples of tissue or cultured cells
Purification of mRNA from 100 g total RNA
Up to 25 mg tissue or up to 5 x 10
6
cells

10 ml buffer for 100 reactions from a variety of
mammalian cell lines
A variety of mammalian cell lines
From a variety of mammalian cell lines
Mammalian cells and tissues including fibrous samples,
yeast and bacteria
Plant samples from a wide variety of species and tissue
types
Isolation of total RNA from mammalian cell lines,
tissues and other samples
Isolation of total RNA from FFPE tissue samples
Isolation of total RNA from laser microdissected samples
& cell lines (LCM); from tissue samples & bacteria (LBR)
Isolation of total RNA from whole blood
Isolation of total RNA from cultured cells and tissue
samples
Isolation of mRNA from tissues and cells and also
from total RNA
Isolation of poly A
+
RNA from total RNA
Isolation of total RNA from tissues and cells
Isolation of total RNA from cartilage tissue and other
tissues

On request

On request

On request

215.-
280.-
63.- / 158.-
757.- / 700.-
69.- / 174.- /
783.-
197.-
1,240.-
1,240.-
1,220.-
149.-
315.-
245.-
251.-
443.-
243.-

100.-
373.-
205.-
225.-
233.-
345.-
620.-
415.-
230.-
175.-
300.-
265.-
240.-
300.-

Complete kit based on a high affinity
Poly T gripNA probe (negatively charged
Peptide Nucleic Acids) and magnetic
beads

--

--

Column-based silica membrane;
On-column DNase treatment
96-well format, silica membrane;
centrifugation or vacuum processing
Phenol/Guanidine-based Lysis Reagent
Isolate concentrated total RNA from the
smallest samples down to a single cell
Purification of DNA-free total RNA from
cultured cells
Designed to produce high yield of pure
total RNA from cells or tissue samples
Produces the highest yield of the purest
mRNA using a fast 20 minute protocol
Designed to produce high yields of pure
miRNA containing total RNA from cells or
tissue sample
Cell lysis and immediate stabilisation of
RNA and DNA for Q PCR analysis
Exceptionally pure mRNA directly from
cells
Quantify RNA directly from cell lysates
Spin column method
Spin column method
Spin column with on-column DNase
digestion
RNA isolation from FFPE tissue without
paraffin removal step
Spin column based extraction
Easy protocol based on several buffer
solutions
Separation with use of oligo dT magnetic
beads / Fast and easy protocol
Uses phenol and chloroform extraction
Uses phenol and chloroform extraction

mTRAP Maxi mRNA
isolation kit
(6 and 5 x 6 reactions)

mTRAP Midi mRNA
isolation kit
mTRAP Total mRNA
isolation kit
PerfectPure
RNA Blood Kit
PerfectPure RNA Cultured
Cell Kit
PerfectPure RNA
RNA Tissue Kit
PerfectPure RNA
Fibrous Tissue Kit
PerfectPure 96 Cell Kit
PerfectPure 96 Cell CS Kit
PerfectPure 96 Cell Vac Kit
Isol-RNA Lysis Reagent
Absolutely RNA
Nanoprep Kit
Absolutely RNA
Microprep Kit
Absolutely RNA
Miniprep Kit
Absolutely mRNA
Purification Kit
Absolutely miRNA Kit

SideStep Lysis and
Stabilization Buffer
Side Step mRNA
Enrichment Kit
Side Step II Cell Lysis
Analysis Kit
Total RNA Isolation Mini Kit

Plant RNA Isolation
Mini Kit
ExpressArt RNAready Kit
ExpressArt FFPE Clear
RNAready Kit
ExpressArt LCM RNAready
ExpressArt LBR RNAready
Whole Blood RNA
Extraction Kit
Broad Range Total
RNA Isolation
Easy mRNA Kit for
RNA Isolation
MagSeq mRNA
Purification Kit
Total RNA Extraction Kit
Cartilage RNA Isolation Kit

Company/Distributor
Amsbio (continued)
Contact: see page 56
Analytik Jena
Life Science
Jena, Germany
www.bio.analytik-jena.de
Contact: lifescience@
analytik-jena.de
Phone +49 36 4177-9400
AppliChem
Darmstadt, Germany
www.applichem.com
Contact:
s.frasch@applichem.com
Phone +49 6151-9357884
Bio & Sell
Feucht, Germany
www.bio-sell.de
Contact: info@bio-sell.de
Phone +49 9128 7243232
BioCat
Heidelberg, Germany
www.biocat.com
Contact: Elke Gamer
gamer@biocat.com
Phone +49-6221-7141516
Isolation of microRNAs and other small RNAs from
cultured cells and tissues
Isolation of total RNA from animal cells, tissue samples,
blood, bacteria, yeast, fungi, plants and nasal or throat
swabs
Isolation of microRNA and small RNAs from cultured
cells, tissue samples, bacteria, plants and blood
Isolation of total RNA from cultured cells
Isolation of total RNA from whole blood
Isolation of total RNA from a large variety of biological
samples
Isolation of total RNA, DNA and protein from tissues
and cells of human, animal, plant, yeast, bacterial and
viral origin
Isolation of total RNA from tissues, cells, bacteria,
plants and yeast
Total RNA from a wide variety of different starting
materials
Total RNA
Total RNA and small RNA molecules, such as mRNA,
tRNA, rRNA, snRNA, miRNA, siRNA
Total cellular RNA from whole blood samples
Total cellular RNA from whole blood samples
Total plant RNA
Viral ssRNA from serum, plasma or other cell-free
bodily fluids, etc.
Total RNA from bacterial culture, mammalian cell
culture, tissue and blood
Total RNA from, bacterial culture, cell culture, tissue,
blood
Total RNA from bacterial culture, cell culture, tissue,
blood
Virus RNA (or DNA) from 350 l fresh or frozen blood
Total RNA from tissue, from eukaryotic cells, from
bacteria
Tissue Monolayers / Suitable for adherent cells and
suspension cells
Gram-negative and Gram-positive bacteria, tissue /
Adherent and suspension cells
Highly contaminated probes also for humin acid con-
tamination probes
Mammalian cells and tissue, blood, plasma/serum,
bacteria, yeast, fungi, plants and more /
All sizes of RNA including microRNA
Mammalian cells and tissue, blood, plasma / serum,
bacteria, yeast, fungi, plants, & more / All sizes of RNA
including microRNA
All types of formalin fixed paraffin embedded tissue /

Blood preserved using PAXgene or Tempus Blood RNA
Tubes / All sizes of RNA including microRNA

175.-
211.-

205.-
385.-
440.-
180.-
185.-

115.-
44.- / 175.- /
700.-
100.- / 270.- /
495.-
44.- / 175.- /
700.-
44.- / 175.- /
700.-
100.- / 270.- /
495.-
50.- / 200.- /
800.-
38.- / 150.- /
600.-
202.70
243.70
88.60 /
296.95
784.75
Starting at
0.99 / reaction
Starting at 69.- /
100 ml
Starting at 2.89
/ reaction
119.90 /
10 reactions
230.- / 419.-
(50 / 100
reactions)
62.- (2 plates,
192 preps)
304.- (50 rxns)

390.- (50 preps)


High-throughput isolation in a 96-well
plate format that use filter plates
High-throughput isolation in a 96-well
plate format that use filter plates
Reagent containing phenol and guani-
dinium thiocyanate
Reagent containing phenol and guani-
dinium thiocyanate
Reagent containing guanidinium salts
and urea
Ready-to-use RNA isolated after only
15 - 40 minutes
Universal, MIDI-format kit for extracting
total RNA
Based on Spin Filter technology
Selective removal of genomic DNA with no
DNase I digestion
Based on the use of MIDI format spin filter
columns
Selection of two integrated lysis buffer
systems
Spin Filter columns with optimised binding
membranes
Silica-based spin columns / Lysis solution
containing chaotropic salts and phenol
Silica-based spin columns /
Lysis with chaotropic salts and phenol
Lysis with chaotropic salts and phenol
Lysis with VirusAC-Solution and phenol
Columns, no shredder necessary /
No DNAse digestion
Guanidinium thiocyanate/Phenol
Columns
Liquid
Spin column chromatography using silicon
carbide resin (SiC)
carbide resin (SiC) / 96-well format for
automation / high throughput applications
Deparaffinisation and Proteinase K
treatment followed by spin column
chromatography using silicon carbide
resin (SiC)
Lysis reaction followed by spin column
chromatography using silicon carbide
resin (SiC)

MicroRNA Isolation Kit
Vantage Total RNA
Purification Kit
Vantage microRNA
purification Kit
EZ-RNA 96 Kit
EZ-Blood RNA 96 Kit
RNA Bee
RNA Stat-60

Ultraspec RNA
innuPREP RNA Mini Kit
innuPREP RNA MIDI
Direct Kit
innuPREP Micro RNA Kit
innuPREP Blood RNA Kit
innuPREP Blood RNA MIDI
Direct Kit
innuPREP Plant RNA Kit
innuPREP Virus RNA Kit
Total RNA Maxi SI Isolation
Spin-Kit
Total RNA Midi SI Isolation
Spin-Kit
Total RNA Mini SI Isolation
Spin-Kit
Virus DNA/RNA Isolation
Spin-Kit
B&S RNA-Mini-Kit
B&S RNA tri-liquid
Isolation
Micro-RNA Kit
B&S RNA High efficiency
Norgen Total RNA
Purification Kit
Norgen Total RNA
Purification 96-Well Kit
Norgen FFPE Total RNA
Purification Kit for Formalin
Fixed Paraffin Embedded
Tissue
Norgen Preserved Blood
RNA Purification Kit I for
Tempus Blood RNA Tubes
or Kit II for PAX Blood RNA
Tubes
Company/Distributor
BioCat (continued)
Contact: see page 57
Chemicell
Berlin, Germany
www.chemicell.com
Contact: Cengiz ztrk
cengiz@chemicell.com
Phone +49-30-2141481
Axygen Bioscience
Corning Life Sciences
Amsterdam, Netherlands
www.axygenbio.com
Contact: Joanne Woodward
WoodwardJ2@corning.com
Phone +44 7788 645 292
GE Healthcare
Life Sciences
www.gelifesciences.com/
sampleprep
Contact: Sally Woods
Please contact your local
GE or distributor customer
service department
Jena Bioscience
Jena, Germany
www.jenabioscience.com
Contact: Brk Schfer
Phone +49 36416285000
info@jenabioscience.com
Life Technologies
Darmstadt, Germany
www.lifetechnologies.com
Contact: Anke Werse
debra.nickson@lifetech.com
Urine samples
Serum or plasma prepared from blood collected on
either EDTA or citrate
Serum, plasma and tumor ascites fluid /
Free circulating RNA fragments including microRNAs
Urine, spinal fluid, tissue culture media /
Cultured animal cells, small tissue samples and
bacterial cells / Small RNA molecules (<200 nt)
Blood, cells, bacteria, viruses

Animal tissue, plant tissue, cultured cells, bacteria,
yeasts, filamentous fungi
See above
See above
Serum, plasma, ascites, cerebrospinal fluid, urine, cell
culture supernatants
Anti-coagulated whole blood

Animal tissue, plant tissue, cultured cells

Cultured cells, tissue, bacteria or yeast
Cultured cells, tissue, bacteria, and yeast

mRNA from a wide variety of eukaryotic cells or tissue
samples, even from a single cell
Total RNA from small amounts of various samples
including blood, animal and plant tissue, bacteria and
viruses

Total RNA from a wide range of cell and tissue types,
including animal, plant, and yeast cells, bacteria and
blood
See above
See above
For samples containing between 1-10 cells

Total RNA, including small RNAs, from virtually all
tissues and cells
Viral RNA from cell-free samples, such as serum,
plasma, and cerebrospinal fluid

450.- (50 x
5 ml preps)
250.- /
12 x 5 ml preps
444.-
(20 reactions)
444.- (10 preps)
188.-
(25 reactions)
30.-
130.-
500.-

48.37
201.63
840.14
27.64 / 100.82
235.23
36.85 / 145.89
464.88
23.74
98.95
445.27
61.04
299.67
1,220.94
23.03
291.24
1,344.23
108.15 / 222.-
967.-
212.-

523.-

24.-
90.-
360.-

223.-

342.-
409.05
1,760.-

302.- to 354.-
180.-

carbide resin (SiC)
carbide resin (SiC)
Exosome isolation followed by column-
based purification of exosomal RNA
Exosome isolation followed by column-
based purification of exosomal RNA
carbide resin (SiC)
Isolation of total RNA/DNA with magnetic
silica particles

The mini spin/vac column allows samples
to be processed using either a centrifuge
or vacuum manifold
See above
See above
Viral nucleic acids are purified by binding
to a special AxyPrep column
The mini spin/vac column allows samples
to be processed using either a centrifuge
or vacuum manifold
A purified cellular RNA subfraction, which
is highly enriched for lower molecular
weight species, such as miRNAs, siRNAs
and tRNAs is produced
Simple and convenient format that is
suitable for all levels of expertise
High-quality total RNA due to the removal
of genomic DNA through on-column
DNase I treatment
Utilise speed and selectivity of oligo(dT)-
cellulose spin column chromatography
to purify high-quality mRNA
Isolation of total RNA by silica-gel
membrane adsorption

Silica-membrane column RNA purification

Combines organic lysis capability of TRIzol
with silica spin-column format
Ready-to-use, monophasic solution of
phenol and guanidine isothiocyanate
Cells are simply lysed at room
temperature in 10 minutes;
an optional DNase digestion can be
performed concurrently during lysis
Organic total RNA extraction followed by
purification on a glass fiber filter
Spin column technology

Norgen Urine Total RNA
Purification Maxi
Kit slurry format
Plasma/Serum Circulating
RNA Purification Kit
SeraMir Exosome RNA
Purification Kit
SeraMir TC Exosome RNA
Purification Kit
Norgen microRNA
Purification Kit
geneMAG-RNA/DNA Kit
(15, 100, 500)

AxyPrep Multisource
RNA MiniPrep Kit,
4, 50, 250 preps/kit sizes
MidiPrep Kit,
MaxiPrep Kit,
AxyPrep Body Fluid Viral
DNA/RNA MiniPrep Kit,
AxyPrep Blood RNA
MiniPrep Kit,
AxyPrep MicroRNA
(miRNA) MiniPrep Kit,
illustra RNAspin Mini Kit
20, 50, 250 preps
illustra RNAspin Midi Kit
20 preps
illustra QuickPrep Micro
mRNA Purification Kit
Total RNA Purification Kit
10, 50, 250 preps

PureLink RNA Mini
50 reactions
TRIzol Plus RNA Purifica-
tion System 50 reactions
TRIzol Reagent 200ml
Ambion Single Cell-to-CT,
50 reactions

Ambion mirVana miRNA
Isolation Kit 40 reactions
PureLink Viral RNA/DNA
Mini Kit

Company/Distributor
Macherey-Nagel
Dueren, Germany
www.mn-net.com
Contact: Michael Bumann
Phone +49 2421969270
or 275
tech-bio@mn-net.com
Merck Millipore
Darmstadt, Germany
www.merck4biosciences.com
Contact: GECustomer
Service@merckgroup.com
Miltenyi Biotec
Berg. Gladbach, Germany
www.miltenyibiotec.com
Contact: macs@
miltenyibiotec.de
MoBiTec
Goettingen, Germany
www.mobitec.com
Contact: Arne Schulz
info@mobitec.com
Phone +49 551-707220
Peqlab Biotechnologie
Erlangen, Germany
www.peqlab.com
Contact: Emanuel Clausing
Phone +49 9131 6107020
info@peqlab.com
PromoCell
Heidelberg, Germany
www.promokine.info
Contact:
info@promokine.info
Phone +49 6221 64934-0
Cells, tissue, bacteria, yeast / Total RNA > 200 nt
Smallest amount of sample material /
Total RNA > 200 nt
Cells, tissue, bacteria, yeast / Total RNA > 200 nt
Cells, tissue, plants, reaction mixtures /
Small RNA (< 200 nt) and large RNA (> 200 nt) in
one or two separate fractions
Plasma, serum / RNA < 1000 nt
Cells, tissue, plants / Total RNA > 200 nt
Cells, tissue, plants / Total RNA > 200 nt
Whole blood (fresh, frozen) / Total RNA > 200 nt
Whole blood (fresh, frozen) / Total RNA > 200 nt
FFPE samples / Total RNA > 200 nt
FFPE samples / Total RNA > 200 nt
Plant material / Total RNA > 200 nt
Phenol/chloroform extracts, reaction mixtures /
Total RNA > 200 nt
RNA containing solutions / Total RNA > 200 nt
The system effectively isolates poly(A)+ RNA from plant
and animal tissues as well as from cultured cells
See above

mRNA from cells, tissue samples, total RNA, or whole
blood

Eukaryotic cells, animal tissue, microbial cells,
plant tissue, blood, small RNAs

Total RNA from a wide variety of cells or tissue
Total RNA from fluidic samples, e.g. suspension cells,
blood, serum, plasma, biological fluids
Total RNA from fat-rich tissue
Total RNA from cells and tissues of human and verte-
brate origin where starting material is limited
Total RNA extraction from fresh or anticoagulated,
e.g. heparin- or EDTA-treated, blood
Total RNA from plant cells and tissues, fungi and yeasts
Total RNA from gram-positive and gram-negative
bacteria
Viral RNA from serum, plasma and biological fluids
Total RNA from samples of human, animal, plant, yeast,
bacterial and viral origin
Total RNA from samples of human, animal, plant, yeast,
bacterial and viral origin

On request
On request
On request
On request

On request
On request
On request
On request
On request
On request
On request
On request
On request
On request
467.-

607.-

On request

16.-
215.-

See website
See website
See website
See website
See website
See website
See website
See website
109.-
119.-


Magnetight Oligo(dT) Particles, that
contain covalently attached oligo(dT)25 to
directly bind mRNA
See above

Direct mRNA isolation from cells, tissue
samples, total RNA, or whole blood using
superparamagnetic Oligo(dT) MicroBeads
and MACS Columns

Column-free selective extraction with an
aqueous solution that inactivates RNases

Ready to use reagent for fast and easy
extraction of high-quality RNA
Ready to use reagent
Reversible binding to PerfectBind silica
spin filters
spin filters
spin filters
spin filters
spin filters
spin filters
Organic extraction and alcohol
precipitation
Organic extraction and alcohol
precipitation / BCP replaces volatile and
toxic chloroform

NucleoSpin RNA II
NucleoSpin RNA XS
NucleoSpin RNA L
NucleoSpin miRNA

NucleoSpin miRNA Plasma
NucleoSpin TriPrep
NucleoSpin RNA/Protein
NucleoSpin RNA Blood
NucleoSpin RNA Blood
Midi
NucleoSpin FFPE RNA
NucleoSpin FFPE
RNA/DNA
NucleoSpin RNA Plant
NucleoSpin RNA Clean-up
NucleoSpin RNA
Clean-up XS
Straight A mRNA Isolation
System
Straight As mRNA Isola-
tion System plus Separa-
tion Stand
MAC mRNA Isolation Kit

AquaRNA
1ml, 30 ml

peqGOLD RNAPure
peqGOLD RNAPur FL
peqGOLD HP Total RNA Kit
peqGOLD MicroSpin Total
RNA Kit
peqGOLD Blood RNA Kit
peqGOLD Plant RNA Kit
peqGOLD Bacterial RNA Kit
peqGOLD Viral RNA Kit
RNA Isolation Kit I
RNA Isolation Kit II

Company/Distributor
Qiagen
Hilden, Germany
www.qiagen.com
Contact: Jrg Dennig
joerg.dennig@qiagen.com
Phone +49 2103 2916326
EURx / Roboklon
www.eurx.com.pl
Gdask, Poland
Roboklon
Berlin, Germany
www.roboklon.com
Roche Diagnostics
Mannheim, Germany
www.roche-applied-
science.com
Contact: Christian Knop
christian.knop@roche.com
Total RNA from cells and easy-to-lyse tissue
(e.g., kidney, liver, spleen), yeast
Total RNA from cells and easy to lyse tissue
(e.g. kidney, liver, spleen), yeast
Total RNA from all types of tissue
Total RNA from animal blood (stabilised)
Cells, saliva, tissue, bacteria
Total RNA from formalin-fixed, paraffin-embedded
(FFPE) tissue sections
Cells, tissue
Total RNA, including miRNA, from all types of tissue
and cells
Total RNA, including miRNA, from formalin-fixed,
paraffin-embedded (FFPE) tissue sections
Total RNA from fresh whole blood; compatible with
common anti-coagulants
Human and animal tissues, cell cultures, plant tissue,
plant liquids, bacteria, yeasts, etc.
Human and animal tissues (solid or liquid, but not
blood), cell cultures, plant tissue, plant liquids
See above

Fresh human blood samples /
Heparin, citrate, EDTA conserved blood samples
Total RNA purification of blood, cultured cells, yeast,
and bacteria (scale: mini)
Total RNA purification of tissue (scale: mini)
Total RNA, DNA and protein purification of cultured cells,
tissues, bacteria, yeast, blood, plant (mini, midi, maxi)
Total RNA purification of 1-10 m FFPE tissue sections
(scale: micro)
Total RNA purification of fresh-frozen or formalin-fixed
paraffin-embedded tissue (scale: mini)
Total RNA and microRNA purification from animal tissue,
cell culture, formalin-fixed, paraffin-embedded tissue,
plant (scale: mini)
Viral RNA purification of serum, plasma, urine, other
body fluids, supernatant from cell culture (scale: mini)
mRNA purification of cultured cells, tissues, total RNA
(scale: mini - midi)
mRNA purification of cultured cells, tissues, total RNA
(scale: micro - mini)
mRNA purification of whole blood/bone marrow
Up to 50 l labelling mixture (Sephadex G-25)
Up to 100 l labelling mixture (Sephadex G-50)

20-75 l labelling mixture

Starting from
252.-
Starting from
286.-
Starting from
174.-
1,675.-
334.-
406.-
Starting from
307.-
366.-
Starting from
423.-
Starting from
321.-
371.-
290.-
48.-
177.-
53.-
197.-
62.-
229.-
53.-

214.50
(Germany)
214.50
121.50 (50 ml)
397.- (200 ml)
302.50
439.-
319.-

257.-
263.-
373.50
451.-
170.-

211.-

RNA purification based on silica-
membrane technology
Silica-membrane technology, including
gDNA Eliminator columns
Silica technology + QIAzol including gDNA
removal
Silica-membrane technology plus QIAzol in
a 96-well format
Silica-membrane technology plus QIAzol
Silica-membrane technology, including
RNA stabilisation
Simultaneous purification of DNA, RNA, pro-
tein based on silica-membrane technology
Silica-based spin columns
Spin column set

Spin column set

Spin column set

Spin column set

Silica adsorption (spin column technology)
Solution-based isolation

Affinity purification
Gel filtration

Gel filtration

RNeasy Kits (Micro, Mini,
Midi, Maxi, 96)
RNeasy Plus Kits (Micro,
Mini, 96)
RNeasy Plus Universal Kits
(Mini, Midi)
RNeasy 96 Universal
Tissue Kit
RNeasy Microarray Tissue
Mini Kit
RNeasy Protect Animal
Blood Kit
RNeasy Protect Kits
RNeasy FFPE Kit
AllPrep Kits
miRNeasy Kits
(Micro, Mini, 96)
miRNeasy FFPE Kit
QIAamp RNA Blood
Mini Kit
Universal RNA Purification
Kit (GeneMatrix) /
25, 100 preps
Universal RNA / miRNA
Purification Kit (GeneMa-
trix) / 25, 100 preps
DNA + RNA + Protein Ex-
traction Kit (GeneMatrix) /
25, 100 preps
Human Blood RNA Purifi-
cation Kit (GeneMatrix) /
25 preps
High Pure RNA Isolation Kit
High Pure RNA Tissue Kit
TriPure Isolation Reagent
High Pure FFPE RNA
Micro Kit
High Pure RNA Paraffin Kit
High Pure miRNA
Isolation Kit
High Pure Viral RNA Kit
mRNA Isolation Kit
mRNA Capture Kit
mRNA Isolation Kit for
Blood/Bone Marrow
Quick Spin Columns for
radiolabelled RNA purifica-
tion, Sephadex G-25 /
Sephadex G-50
Mini Quick Spin
RNA Columns

Company/Distributor
Stratec Molecular
Berlin, Germany
www.invitek.de
Contact:
info.berlin@stratec.com
Phone +49 30 9489 2901
or 2910
Sd-Laborbedarf
Gauting, Germany
www.suedlabor.de
Contact: Uli Schaffar
Phone: +49 898506527
info@suedlabor.de
Takara/Clontech
Takara Bio Europe
St-Germain-en Laye, France
www.takara-bio.eu
Contact: Francois-X. Sicot
tech@clontech-europe.com
Phone +33 1 3904 6880
(Europe)
Thermo Fisher
Scientific
St. Leon-Rot, Germany
www.fermentas.com
Contact: Luiz Correa
Techservice.emea.
genomics@thermofisher.
com
Phone +49 6227-356790
Total RNA from cells, tissue, FFPE tissue slices and
blood samples
Total RNA from mammalian cell cultures, Gram-positive
and Gram-negative bacteria cells and yeast cells
Total RNA from up to 20 mg human or animal tissue
and paraffin embedded tissue samples
Total RNA from up to 1.5 ml of whole blood, 0.3 ml
buffy coat, up to 1 x 10
7
lymphocyte pellets
Total RNA from 100 mg plant material or 10
7
plant cells
Co-purification of viral RNA from phytopathogens
Total RNA from 5 x 10
5
human and animal cells

Total RNA from 1 - 10 mg tissue sample
Total RNA from up to 50 mg of plant material

Viral RNA from plasma, serum, blood, tissue,
stool samples, mammalian cells, cell culture
supernatants, rinse liquid from swabs
Viral RNA from 200 l of virus containing samples of
serum and plasma or 100 l blood samples
Viral RNA (and DNA) from up to 200 l of serum, plasma,
other cell-free body fluids, cell culture supernatants, etc.
Viral RNA from up to 200 l of plasma, serum,
cell-free body fluids of human origin, etc.
Viral RNA from up to 200 l of plasma, serum,
cell-free body fluids of human origin, etc.
Most difficult plant types, including strawberry leaf,
cotton leaf, cotton seeds and pine needles
Total RNA from all common soil types
Nucleic acids from biofilm samples

RNA from filtered water samples, including turbid waters
Up to 100 l of RNA products
Mammalian cells or total RNA

96-well cell culture should be used as sample to
prepare RNA lysate
96-well cell culture should be used as sample to pre-
pare RNA and proteins lysate
Total RNA from mammalian cell cultures and tissues,
whole blood, bacteria, yeast, insects
Total RNA from whole blood and related body fluids,
bone marrow, buffy coat
Total RNA as well as mitochondrial, chloroplastidial, viral
and bacterial RNA from a wide variety of plant species
Viral nucleic acids from various human and animal
liquids
Viral nucleic acids from human plasma or serum sam-
ples for in vitro diagnostic purposes

202.- / 803.-
(Germany)
185.50
781.50
173.50
746.50
185.50
781.50
226.-
888.-
449.-
788.50
4,604.-
468.- / 858.-
4,640.-
708.50
4,088.-
152.-
658.-
375.-
275.-
164.-
689.-
415.- / 735.-
4,337.-
400.-
716.-
4,254.-
4.95 /prep
11.- /prep
8.50.- /prep

7.90 /prep
1.42 /prep
375.-

180.-

190.-

175.- / 750.-
(Germany)
200.-
(Germany)
200.- / 800.-
(Germany)
165.-
(Germany)
200.-
(Germany)

Membrane adsorption
See above
See above
See above
See above
Membrane adsorption, filter plates


Membrane adsorption in capped spin
columns
Purification with spin columns

Pre-filled extraction tubes /
Purification with spin columns
Membrane adsorption, filter plates;
96-well format, using a vacuum manifold
Bead beating technology combined with
silica spin filter tubes
Inhibitor Removal Technology (IRT) for iso-
lating total RNA from up to 2 grams of soil
Chemical and mechanical lysis; Inhibitor
Removal Technology (IRT) to remove the
concentrated inhibitors
Bead tubes for optimal lysis and Inhibitor
Removal Technology (IRT)
RNA Pure Buffer ensures easy binding of
DNA to the glass fiber matrix of spin column
Direct RNA prep and cDNA synthesis to
prepare template from few cells (2-200) or
limited RNA amount (< 20 ng)
Direct preparation of RNA template to be
analysed by RT-qPCR (one or two step)
Direct preparation of RNA template to be
analysed by RT-qPCR and proteins for
western blot analysis
Silica-based membrane technology in the
form of a convenient spin column
Silica-based membrane spin column



InviTrap Spin Universal RNA
Mini Kit / 50, 250 preps
InviTrap Spin Cell RNA Mini
Kit / 50, 250 preps
InviTrap Spin Tissue RNA
InviTrap Spin Blood RNA
InviTrap Spin Plant RNA
InviTrap RNA Cell HTS 96
Kit/C / 2 x 96, 4 x 96,
24 x 96 preps
InviTrap RNA Tissue HTS
96/C / 2 x 96
InviTrap RNA Plant
HTS 96 Kit/C /
4 x 96, 24 x 96 preps
Invisorb Spin Virus RNA
RNAsure Virus System (RNA-
sure Spin Virus Kit + Protec-
tion Module I) / 50 preps
RTP DNA/RNA Virus Mini
Kit / 50, 250 preps
Invisorb Virus RNA
HTS 96 Kit/C
Invisorb Virus RNA HTS
96 Kit/V / 2 x 96, 4 x 96,
24 x 96 preps
PowerPlant RNA
Isolation Kit
RNA PowerSoil Total RNA
Isolation
PowerBiofilm RNA
Isolation Kit
PowerWater RNA
Isolation Kit
Hi Yield RNA Pure Kit
CellAmp Whole Transcrip-
tome Amplification Kit
(Real Time) / 50 preps
CellAmp Direct RNA Prep
Kit for RT-PCR (Real Time)
/ 200 preps
CellAmp Direct Prep Kit for
RT-PCR (Real Time) & Pro-
tein Analysis / 200 preps
GeneJET RNA Purification
Kit / 50, 250 preps
GeneJET Whole Blood RNA
Purification Mini Kit /
50 preps
GeneJET Plant RNA Purifica-
tion Mini Kit/50, 250 preps
GeneJET Viral DNA and
RNA Purification Kit /
50 preps
Viral DNA/RNA Purification
Kit / 50 preps

Old and New Ways To RNA

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page 52 Lab Times 2-2012 Products

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