Name: _______________________ Course&Sec: _____________ Group No: _________

Date Performed: _______________ Date Submitted: __________ Score: _____________

Activity No: ____________________ Instructor: _________________________________


3. Put about 12mL of CBB in the petri dish and cover it.
4. Place the cut potato on a slide. Make thin sections of the potato by cutting it with two
razor blades held tightly with your fingers. The distance between the two razor blades held
this way is 0.3 mm.
5. Using the pincette remove the thin section located in between the razor blades. Place it
in a petri dish with water. A good section appears almost transparent when placed in
water.
6. Make several sections. Wipe the razor blades before cutting another thin section. Place
the thin sections in the petri dish with water.
7. Soak the thin sections in CBS for 30 minutes.
8. After 30 minutes, place a good section on another slide. Put a cover slip over it.
9. Observe under the LPO and HPO of the microscope. Find the blue stained portions in
the cells. These are proteins in solution form.

Question 1: Draw some cells with proteins as observes under the HPO. Color and label
them.

100x (LPO) 400x(HPO)
10. Now, put a piece of filter or tissue paper on one side of the cover slip. Then add about
four drops of iodine solution on the other side.
Safety Precaution: Be careful in handling stains. They leave mark that are difficult to
remove.
11. After 5 minutes, examine samples under the LPO and HPO of the microscope
Question 2. Which of the two stained structures is starch? Explain why. _______________
________________________________________________________________________
________________________________________________________________________

Question 3. How does the protein differ in form from starch in potato cells? ____________
________________________________________________________________________
________________________________________________________________________
Name: _______________________ Course&Sec: _____________ Group No: _________
Date Performed: _______________ Date Submitted: __________ Score: _____________
Activity No: ____________________ Instructor: _________________________________

Question 4: Draw a few cells showing protein and starch as observed under the HPO.
Color and label them.


100x(LPO) 400x(HPO)

Question 5: Which is more abundant in potato cells, protein or starch? ________________

RESULTS AND DISCUSSION









GENERALIZATION










Name: _______________________ Course&Sec: _____________ Group No: _________
Date Performed: _______________ Date Submitted: __________ Score: _____________
Activity No: ____________________ Instructor: _________________________________

3. Get one good section and put it on another slide. Add several drops of Sunda III until the
section is evenly covered by the stain.

Safety Precaution: Handle stains with care. They can leave persistent mark on your hands
and clothes!

4. Put a cover slip over the specimen. Set aside for 25 minutes. Add more Sudan III when
you notice that the section is not evenly covered by the stain.
5. After 25 minutes, remove the cover slip. Wash the section with 50% ethyl alcohol ass
shown in figure 5.3A. Blot dry the specimen using strips of filter paper (Figure 5.3B).
6. Add two drops of glycerin to the stained section. Put a cover slip over it.
7. Observe the specimen under the low and high power objectives of the microscope. Look
for the red stained oil globules within the cell. Oil is a kind of lipid.

Question 1. How much are the cells magnified under the LPO and HPO? ______________
________________________________________________________________________
Question 2. Draw some cells with oil globules. Label and color the parts of the cells.

100x(LPO) 400x(HPO)

B. For Coconut Endosperm
1. Cut a 2 x 1 cm piece of the coconut endosperm (Fig. 5.4 A). Remove the brown/black
outer covering.
2. Place the cut piece of endosperm on a slide. Cut very thin longitudinal sections using a
new razor blades or a hand microtome.
3. Follow steps 3 to 6 of procedure A.
4. Observe the specimen under the LPO and HPO of the microscope. Identify the lipid.


Name: _______________________ Course&Sec: _____________ Group No: _________
Date Performed: _______________ Date Submitted: __________ Score: _____________
Activity No: ____________________ Instructor: _________________________________

Question 3. Draw some cells. Label the parts. Color the lipid.

100x(LPO) 400x(HPO)

Question 4. Describe the lipids that you observe in peanut and coconut endosperm.
________________________________________________________________________
________________________________________________________________________
________________________________________________________________________

Question 5. What roles do lipids play in human body?
________________________________________________________________________
________________________________________________________________________

RESULTS AND DISCUSSION







GENERALIZATION







Name: _______________________ Course&Sec: _____________ Group No: _________
Date Performed: _______________ Date Submitted: __________ Score: ___________
Activity No: ____________________ Instructor: _________________________________

Table 6.1
Starch Solution Temperature Starch Suspension
+ Iodine
Starch Suspension
+Amylase + Iodine
A 5
o
C
B
C 80
o
C

11. Add a drop of Iodine solution to each sample. Describe the color of the reactions in
Table 6.1

Question 1. Based on your results, explain the effect of temperature on amylase activity


RESULTS AND DISCUSSION






GENERALIZATION





Name: _______________________ Course&Sec: _____________ Group No: _________
Date Performed: _______________ Date Submitted: __________ Score: ___________
Activity No: ____________________ Instructor: _________________________________

Activity 7
Determining the Effect of pH on Amylase Activity

INTRODUCTION
pH is a measure of acidity or alkalinity of a solution or a culture medium. It is a
measure of the activity of the hydrogen ion concentration
Some enzymes are active at low pH or acid values. Some enzymes are active at
high pH or alkaline values. Most enzymes work best in the neutral range.
In this activity, you will determine the effect of pH on amylase activity.

OBJECTIVE
To determine the effect of pH on amylase activity

MATERIALS
Test tubes 0.5% Starch suspension- pH 3.0, 6.0, 9.0
Test tube rack 0.1% Amylase solution (or Fresh Raddish Extract)
Graduated cylinder Iodine solution
Spot plate pH paper
Pasteur pipettes marking pen
Medicine droppers

PROCEDURE
1. Label three test tubes as A, B and C.
2. Label three spot plates as A, B and C.
3. Add 10 drops of amylase solution to each test tube. Mix well. Set aside the three test
tubes at room temperature for 10 minutes.
4. Get a drop of the mixture from each test tube and put it on a spot plate.
5. Add a drop of the iodine solution to each sample
6. Indicate the color of each reaction and record the results in table 7.1.

Table 7.1
Starch Solution pH Starch suspension +iodine Starch suspension +
Amylase + iodine
A 3.0
B 6.0
C 9.0

Name: _______________________ Course&Sec: _____________ Group No: _________
Date Performed: _______________ Date Submitted: __________ Score: ___________
Activity No: ____________________ Instructor: _________________________________

Question 1. Based on your results, explain the effect of pH on amylase activity.







RESULTS AND DISCUSSION