7/16/2014 Trigonella foenum-graecum L.

Seed Oil Obtained by Supercritical CO2 Extraction - Springer

http://link.springer.com/article/10.1007/s11746-012-2123-x 1/8
Look Inside Get Access
Find out how to access preview-only content
Journal of the American Oil Chemists' Society
December 2012, Volume 89, Issue 12, pp 2269-2278
Trigonella foenum-graecum L. Seed Oil Obtained by
Supercritical CO
2
Extraction
Abstract
Supercritical CO
2
extraction (SC-CO
2
) of fenugreek (Trigonella foenum-graecum L.) seed oil and its
chemical composition and antioxidant activity were investigated. A central composite design combined with
response surface methodology was used to study extraction conditions including pressure, temperature, and time.
The optimum extraction conditions were 28.5 MPa extraction pressure, 41 C extraction temperature, and
118 min extraction time, where 3.78 % yield was predicted. Fenugreek seed oil extracted under optimum
conditions by SC-CO
2
was mainly composed of 28.3 % C18:3, 33.45 % C18:2, 9.89 % C16, 8.1 % C18:1,
3.7 % C18, 0.71 % C20, and 0.61 % C22. The fenugreek oil was rich in unsaturated fatty acids (nearly 70 %
of the total fatty acids), and polyunsaturated fatty acids accounted for 61.42 % (mass percentage) of the total
amount. The 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity increased from 12.5 to 88.4 % when the
concentration was increased from 1 to 12 mg/ml. The reducing power of the seed oil was concentration-
dependent. The antioxidant activity of the supercritical fluid extraction extract was superior to those obtained by
Soxhlet extraction.
Citations
American Oil Chemists' Society
Within this Article
7/16/2014 Trigonella foenum-graecum L. Seed Oil Obtained by Supercritical CO2 Extraction - Springer
http://link.springer.com/article/10.1007/s11746-012-2123-x 2/8
1. Introduction
2. Experimental Procedures
3. Results and Discussion
4. Conclusions
5. References
6. References
7/16/2014 Trigonella foenum-graecum L. Seed Oil Obtained by Supercritical CO2 Extraction - Springer
http://link.springer.com/article/10.1007/s11746-012-2123-x 3/8
Related Content
References (23)
1. Kaufmann B, Rudaz S, Cherkaoui S, Veuthey JL, Christen P (2007) Influence of plant matrix on
microwave-assisted extraction process. The case of diosgenin extracted from fenugreek
(Trigonella foenum-graecum L.). Phytochem Anal 18:7076 CrossRef
2. Rayyan S, Fossen T, Andersen M (2010) Flavone C-glycosides from seeds of fenugreek,
Trigonella foenum-graecum L. J Agric Food Chem 58:72117217 CrossRef
3. Wang GR, Tang WZ, Yao QQ, Zhong H, Liu YJ (2010) New flavonoids with 2BS cell
proliferation promoting effect from the seeds of Trigonella foenum-graecum L. J Nat Med
64:358361 CrossRef
4. Pollard M, Eder B, Fischer P, Windhab E (2010) Characterization of galactomannans isolated
from legume endosperms of Caesalpinioideae and Faboideae subfamilies by multidetection
aqueous SEC. Carbohydr Polym 79:7084 CrossRef
5. Gopu C, Gilda S, Paradkar A, Mahadik K (2008) Development and validation of a densitometric
TLC method for analysis of trigonelline and 4-hydroxyisoleucine in fenugreek seeds. Acta
Chromatogr 20:709719 CrossRef
6. Haeri MR, Izaddoost M, Ardekani MRS, Nobar MR, White KN (2009) The effect of fenugreek
4-hydroxyisoleucine on liver function biomarkers and glucose in diabetic and fructose-fed rats.
Phytother Res 23:6164 CrossRef
7. El-Soud NHA, Khalil M, Hussein J, Oraby F, Farrag ARH (2007) Antidiabetic effects of
fenugreek alkaliod extract in streptozotocin induced hyperglycemic rats. J Appl Sci Res 3:1073
1083
8. Kaviarasan S, Sundarapandiyan R, Anuradha CV (2008) Protective action of fenugreek
(Trigonella foenum graecum) seed polyphenols against alcohol-induced protein and lipid
damage in rat liver. Cell Biol Toxicol 24:391400 CrossRef
9. Shabbeer S, Sobolewski M, Kachhap S, Davidson N, Carducci MA, Khan S (2009) Fenugreek:
a naturally occurring edible spice as an anticancer agent. Cancer Biol Ther 8:272278 CrossRef
10. Dixit P, Ghaskadbi S, Mohan H, Devasagayam T (2005) Antioxidant properties of germinated
fenugreek seeds. Phytother Res 19:977983 CrossRef
7/16/2014 Trigonella foenum-graecum L. Seed Oil Obtained by Supercritical CO2 Extraction - Springer
http://link.springer.com/article/10.1007/s11746-012-2123-x 4/8
11. Chatterjee S, Variyar PS, Sharma A (2010) Bioactive lipid constituents of fenugreek. Food Chem
119:349353 CrossRef
12. Sulieman AME, Ali AO, Hemavathy J (2008) Lipid content and fatty acid composition of
fenugreek (Trigonella foenum-graecum L.) seeds grown in Sudan. Int J Food Sci Technol
43:380382 CrossRef
13. Kavishree S, Hemavathy J, Lokesh BR, Shashirekha MN, Rajarathnam S (2008) Fat and fatty
acids of Indian edible mushrooms. Food Chem 106:597602 CrossRef
14. Fan YY, Chapkin RS (1998) Importance of dietary gamma-linolenic acid in human health and
nutrition. J Nutr 128:14111414
15. Wang LJ, Weller CL (2006) Recent advances in extraction of nutraceuticals from plants. Trends
Food Sci Technol 17:300312 CrossRef
16. Senyay-Oncel D, Ertas H, Yesil-Celiktas O (2011) Effects of supercritical fluid extraction
parameters on unsaturated fatty acid yields of Pistacia terebinthus berries. J Am Oil Chem Soc
88:10611069 CrossRef
17. Li GL, Zhang XL, You JM, Song CH, Sun ZW, Xia L, Suo YR (2011) Highly sensitive and
selective pre-column derivatization high-performance liquid chromatography approach for rapid
determination of triterpenes oleanolic and ursolic acids and application to Swertia species:
optimization of triterpenic acids extraction and pre-column derivatization using response surface
methodology. Anal Chem Acta 688:208218 CrossRef
18. Liu S, Yang F, Zhang C, Ji H, Hong P, Deng C (2009) Optimization of process parameters for
supercritical carbon dioxide extraction of Passiflora seed oil by response surface methodology. J
Supercrit Fluid 48:914 CrossRef
19. Eller FJ, Cermak SC, Taylor SL (2011) Supercritical carbon dioxide extraction of cuphea seed
oil. Ind Crops Prod 33:554557 CrossRef
20. Amarowicz R, Pegg RB, Rahimi-Moghaddam P, Barl B, Weil JA (2004) Free-radical scavenging
capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem
84:551562 CrossRef
21. Gulcin I (2010) Antioxidant properties of resveratrol: a structure-activity insight. Innov Food Sci
Emerg Technol 11:210218 CrossRef
22. Ahmadi F, Sadeghi S, Modarresi M, Abiri R, Mikaeli A (2010) Chemical composition, in vitro
anti-microbial, antifungal and antioxidant activities of the essential oil and methanolic extract of
Hymenocrater longiflorus Benth., of Iran. Food Chem Toxicol 48:11371144 CrossRef
7/16/2014 Trigonella foenum-graecum L. Seed Oil Obtained by Supercritical CO2 Extraction - Springer
http://link.springer.com/article/10.1007/s11746-012-2123-x 5/8
23. Isabel CFRF, Baptista P, Vilas-Boas M, Lillian B (2007) Free-radical scavenging capacity and
reducing power of wild edible mushrooms from northeast Portugal: individual cap and stipe
activity. Food Chem 100:15111516 CrossRef
About this Article
Title
Trigonella foenum-graecum L. Seed Oil Obtained by Supercritical CO
2
Extraction
Journal
Journal of the American Oil Chemists' Society
Volume 89, Issue 12 , pp 2269-2278
Cover Date
2012-12-01
DOI
10.1007/s11746-012-2123-x
Print ISSN
0003-021X
Online ISSN
1558-9331
Publisher
Springer-Verlag
Additional Links
Register for Journal Updates
Editorial Board
About This Journal
Manuscript Submission
Topics
Agriculture
Food Science
Biomaterials
Industrial Chemistry/Chemical Engineering
Biotechnology
Keywords
7/16/2014 Trigonella foenum-graecum L. Seed Oil Obtained by Supercritical CO2 Extraction - Springer
http://link.springer.com/article/10.1007/s11746-012-2123-x 6/8
Fenugreek (Trigonella foenum-graecum L.)
Seed oil
Supercritical carbon dioxide extraction
Response surface methodology
HPLC-FLD-MS
Antioxidant activity
Industry Sectors
IT & Software
Biotechnology
Electronics
Oil, Gas & Geosciences
Engineering
Aerospace
Telecommunications
Chemical Manufacturing
Automotive
Materials & Steel
Energy, Utilities & Environment
Consumer Packaged Goods
Pharma
Authors
Renming Yang
(1)

(2)
Honglun Wang
(1)
Nianhua Jing
(1)

(2)
Chenxu Ding
(1)
Yourui Suo
(1)
7/16/2014 Trigonella foenum-graecum L. Seed Oil Obtained by Supercritical CO2 Extraction - Springer
http://link.springer.com/article/10.1007/s11746-012-2123-x 7/8
Jinmao You
(1)

(3)
Author Affiliations
1. Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest
Plateau Institute of Biology, Chinese Academy of Sciences, 23 Xinning Road, 810001,
Xining, Qinghai, Peoples Republic of China
2. Graduate School of the Chinese Academy of Sciences, 100039, Beijing, Peoples
Republic of China
3. College of Chemistry Science, Qufu Normal University, Qufu, 273165, Shandong,
Peoples Republic of China
Continue reading...
To view the rest of this content please follow the download PDF link above.
Over 8.5 million scientific documents at your fingertips
Springer, Part of Springer Science+Business Media
7/16/2014 Trigonella foenum-graecum L. Seed Oil Obtained by Supercritical CO2 Extraction - Springer
http://link.springer.com/article/10.1007/s11746-012-2123-x 8/8