Animal pregnancy diagnostic kit

PROGESTERONE a female sex hormone increase its

level during pregnancy and maintain its high level
throughout the entire gestation period.

Just after ovulation Corpus Luteum formed in the
ovary which produce Progesterone hormone (P4)

High levels of Progesterone indicates pregnancy
and low level reveals non-pregnancy

Bovipreg test was optimized to determine the
level of progesterone hormone in MILK or
BLOOD between 18 to 22 days post
insemination.


This test is a Quantitative test detects
level of progesterone in Milk or Blood
Serum.

Analytical sensitivity : 2ng/ml
Sensitivity : 96.9% ,
Specificity : 97.8%

Role of Hormones during Estrus Cycle
Higher concentration of progesterone after
insemination gives confirmation that cow is
pregnant.
Accuracy :
Positive Pregnancy : 96.9%
Negative Pregnancy : 97.8%
Detection of Progesterone is
the only method to detect
Early Pregnancy in the animal
just after 18
th
day post
insemination

This test detect accurate
pregnancy in recipients
after 11-15 days of post
Embryo Transplant
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SPECIMEN COLLECTION AND PREPARATION

For Dairy / Beef Cow, Sheep and Goat (Lactating Animal), Camel, Mares

Take whole milk to test its progesterone level directly. In order to get
reliable result, give up the first three milking and then collect sample
milk around 5-10ml. Mix the sample milk well before testing. Pour 2-3
drops Milk.
Sometimes milk with high viscosity prevents its spreading to all areas of
the kit. So, the specimen could not go up to the whole membrane
and apparently stopped in the middle of the test . To solve this problem,
you will need to keep the milk still (at rest) for half an hour after
collection, and then draw the milk from the bottom layer using the
dropper and use that milk on the test kit. Please note that the milk cream
usually floats on the surface.
In this condition pour 3-5 drops of Milk.

SPECIMEN COLLECTION AND PREPARATION
For Heifer/Lactating/Non-Lactating animal
Blood withdrawn and collect it in a collection tube, left
it in horizontal position for 30 minutes, then left it
vertical for another 30 minutes, till blood clot. A clear
liquid appear over the clot. Collect the serum in the
dropper and pour 3-4 drops on the circular well.

Keep Horizontal
Position for 30
Minutes
After 30 Minutes
Keep Vertical
Position for 30
Minutes
Serum
PROCEDURE

1. Remove the test device and dropper from its foil wrapper by tearing
along the slice.

2. Using the dropper, withdraw the milk or blood serum sample from the
specimen cup and slowly dispense 3 4 drops into the circular sample
well. The sample liquid moves slowly to the other end due to the
capillarity. You will see one or two red lines in the result window.

3. Read results at 5 minutes.(in high viscosity of milk sometime it takes
5-7minutes )
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A schematic diagram for the simple procedure
Bovipreg
INTERPRETATION OF RESULTS







Positive: If both T band and C band are visible, the test result is Positive
which means the cow is pregnant or in middle period of estrous cycle.
Negative: If only C line is visible, the test result is negative, which
indicates the cow is not pregnant.
Invalid: When control line does not appear on the membrane, the test is
invalid due to improper test procedure or deterioration of reagents. It is
recommended that the test be repeated.
PRECAUTION
(1) Test according to the law of the estrous cycle
strictly.

(2) For single use only; do not reuse the test devices.

(3) Dont keep the strip or cassette in the air for a long
time after unpacking.

(4) Do not use test kit after expiry date.
VALIDITY AND STORAGE

The period of the validity is 24 months.
Store as packaged in the sealed pouch under the
room temperature.
Avoid light.
We recommend 5 consecutive test for those
cows where we do not have sufficient
information regarding the date of
insemination, during this test if one result
comes to negative then you will be confirmed
that the animal is not pregnant, otherwise
pregnancy will be establish.
To detect early pregnancy please perform ONE test
between 18
th
-22
nd
days after insemination date.

These kits can be stored in normal room temperature
or can be transport anywhere in room temperature.

During early pregnancy test if found negative (OPEN),
wait for the heat(Estrus) to come within 21-22
nd
days
and re-inseminate them without wasting any day.

A. COWSIDE ANYBODY CAN DO THE TEST
B. EASY TO PERFORM THE TEST
C. TEST TAKE 5 MINUTES TO GET THE RESULT
D. NON-INVASIVE DOES NOT INJURE THE ANIMAL
E. DETECT EARLY PREGNANCY
F. USE IN VARIUOS REPRODUCTIVE MANAGEMENT
// Estrus Detection // Embryo Transplant // Non-cycling
cow// Endocrine treatment