Imagine that following translation, the carboxy terminal amino acid of the cell surface insulin

receptor is hydroxylated by a cytosolic enzyme. After insertion of the receptor into the plasma
membrane, what fluid compartment is bathing this modified amino acid?
a. cytosol *
b. ECF
c. lipid region of bilayer

When a mRNA emerges from the nucleus in to the cytosol, it will bind to a ribosome and
translation will begin. Which of the following statements is true of proteins destined for
secretion?
A. The ER signal sequence will bind to the SRP receptor on the ER membrane
B. The ER signal sequence will bind to the SRP which will take the ribosome to a receptor
on the ER membrane*
C. Glycosylation occurs exclusively in the Golgi
D. If they have a KDEL or KKXX amino acid sequence they will be transported to the basal
cell membrane

Which of the following coat proteins is involved in receptor mediated endocytosis of vesicles
containing the LDL receptor ?
A. Clathrin*
B. COP I
C. COP II
D. Caveolin

A group of cells in culture have a defect in the Ran protein in which Ran-GTP cannot be
hydrolyzed to Ran-GDP. Which of the following would most likely be observed as a result?
Excess of.
a. Cytoplasmic Ran-Exportin-cargo complexes *
b. Cytoplasmic Ran-Importin-cargo complexes
c. Nuclear Ran-Export-cargo complexes
d. Nuclear Ran-Importin-cargo complexes

ARF-GEF acts upon its substrate ARF, a small G-protein, to:
a. accelerate the hydrolysis of GTP
b. assist in the release of GDP and the binding of GTP *
c. bind to importin complex and direct it through the nuclear pore complex
d. dissociate the exportin-protein complex in the cytoplasm
e. bring the vesicle membrane close to a target membrane









A 3 year old male is brought to your paediatric clinic, presenting with physical and mental
deterioration. The child was normal at birth, but developmental delays and deterioration were
first noticed several months ago. On physical examination you find organomegaly, a mild
degree of hirsutism, and deafness. There is no clouding of the cornea. Both parents are in good
health and there is no family history of any disorders. Given the clinical findings, you suspect
that the child is suffering from a lysosomal storage disorder and order the relevant tests.
Enzyme assays reveal that iduronodate sulphatase is absent, all other lysosomal enzymes are
present within normal range. What is your diagnosis?

A. Pseudo-Hurler disease
B. Scheie syndrome
C. Hunter syndrome*
D. Hurler syndrome
E. I-cell disease


In I-cell disease lysosomes are often devoid (empty) of soluble enzymes. Which of the
following tags/signal sequences are mutated or absent?

a. ER signal sequence
b. mannose-6-phosphate*
c. secretory signal sequence
d. SKL
e. KKXX

Cells biopsied from a 36-year-old female with breast cancer were identified as estrogen receptor
positive. Which of the following most correctly describes the class of receptors to which
estrogen receptors belong?
a. G-protein Coupled Receptors
b. Intracellular Receptors *
c. Receptor Tyrosine Kinases
d. Receptor Serine/Threonine Kinases

A 5-year-old boy presents with a blistering skin condition. The pediatrician diagnoses his
condition as epidermolysis bullosa simplex after genetic analysis reveals a mutation in a gene for
that most likely encodes which group of proteins?

a. Microtubules
b. Microfilaments
c. Intermediate filaments *
d. Actin-related proteins (Arp)
e. Kynesin motors



A 4 year old male patient presents with increasing difficulty walking, is positive for Gowers
sign, and you notice calf pseudohypertropy. He has mild cognitive impairment and further
consultation with the parents reveals that his 15 year old maternal cousin has the same condition
and has been wheelchair-bound for the last 2 years. Your patient has inherited a gene mutation
that encodes for a protein that binds to ____________ in the membrane?
A. Dystrophin
B. Dystroglycan*
C. Integrin
D. Emerin
E. Actin

After activation of a calcium dependant cell signaling pathway, PKC is activated. Which second
messenger or protein is needed for PKC activation?
a. STAT
b. cAMP
c. RAS
d. DAG*
e. GRB2
f. CREB

Which one of the following drugs/proteins will inhibit tubulin polymerisation?
A. Phalloidin
B. Allopurinol
C. Colchicine*
D. Cofilin
E. Kinesin

Which of the following proteins are involved in creating communicating (gap) junctions?
A. Claudin
B. Cadherin
C. Actin
D. Catenin
E. Connexin*

A ribosomal defect where the ribosomes are unable to attach to the ER membrane would result in
which of the following?
A. Increased production of lysosomal enzymes
B. Decreased production of secretory glycoproteins*
C. Global decrease in glycoprotein synthesis
D. Increased secretion of glycoproteins
E. Decreased production of mitochondrial enzymes