Cell Dyn 3500

HOST INTERFACE SPECIFICATION
CELL-DYN
3500 SYSTEM
includes Reticulocyte Parameters
LIST NO. 03H50-01
REVISION F
ABBOTT DIAGNOSTICS DIVISION
Abbott Laboratories
Abbott Park, IL 60064
TABLE of CONTENTS
INTRODUCTION
DATA
RS-232 TRANSPORT
Q
Q
Q
Q
INTERFACE SPECIFICATION
CELL-DYN
3500 SYSTEM
includes Reticulocyte Parameters
Abbott Laboratories 1996, 1998, 1999 Abbott Diagnostics Division
Abbott Park, IL 60064 03H50-01F May 1999
CELL-DYN
3500 System Host Interface Specification with Reticulocyte Option i

03H50-01F May 1999
Table of Contents
1.0 INTRODUCTION.................................................................................................... 1
1.1 Purpose..................................................................................................... 1
1.2 Definitions and Conventions...................................................................... 1
1.3 Scope........................................................................................................ 1
2.0 DATA..................................................................................................................... 1
2.1 General Message Form............................................................................. 1
2.2 Instrument Messages................................................................................ 2
2.3 Host Messages ....................................................................................... 19
3.0 RS-232 TRANSPORT.......................................................................................... 22
3.1 Mechanical Interface ............................................................................... 22
3.2 Electrical Interface................................................................................... 22
3.3 Data Link................................................................................................. 22
ii CELL-DYN
3500 System Host Interface Specification with Reticulocyte Option

03H50-01F May 1999
NOTES
CELL-DYN
3500 System Host Interface Specification with Reticulocyte Option 1

03H50-01F May 1999
1.0 INTRODUCTION
1.1 Purpose
This document describes the communication methods and interface characteristics of
Abbott CELL-DYN 3500 automated hematology analyzer when connected to a Host.
1.2 Definitions and Conventions
The Host is an external computer or data collection system. The Instrument is the Abbott
CELL-DYN 3500 System.
Data transferred from the Host to the Instrument is called Host Data. Data transferred
from the Instrument to the Host is called Instrument Data.
Numeric Equivalents of characters are shown as hexadecimal values.
1.3 Scope
This document is divided into two major sections, DATA and RS-232 TRANSPORT.
This DATA section describes 2 communication protocols: one for transmitting data while in
Hematology mode and one for transmitting data while in Retic mode.
The RS-232 TRANSPORT section describes the means by which the Instrument and
Host transfer data to each other.
2.0 DATA
This section describes the data that is transferred from the Host to the Instrument and
from the Instrument to the Host. The syntax and meaning of communication messages is
described without reference to the underlying transport mechanism.
2.1 General Message Form
2.1.1 Messages
Data is communicated in discrete, independent messages. Each message
comprises one or more fields composed of printable ASCII characters.
2.1.2 Flow Control
Flow control characters are used to respond to the receipt of messages. These
are the non-printable ASCII characters ACK, NAK, DEL, and CAN.
2.1.3 Syntax and Functional Error Response
The Instrument accepts all messages that the Host successfully transmits. If a
Host message contains syntax or functional errors, the appropriate flow control
character is sent to the Host. Symmetrically, the appropriate flow control character
is sent by the Host in response to an Instrument message that contains syntax or
functional errors.
2 CELL-DYN

03H50-01F May 1999
2.1.4 Syntax of Host and Instrument Messages
Although Host and Instrument messages convey different information,
they share a common syntax:
2.1.4.1 Each message is partitioned into fields separated by commas.
2.1.4.2 All fields are of fixed length.
2.1.4.3 Text fields begin and end in double quotation marks (") and use
the blank character to occupy empty positions. RTC message
Type floating point values are transmitted as text.
2.1.4.4 Numeric floating point fields are right-justified, and zeroes are
used to fill the empty positions. Numeric integer fields are right-
justified, and zeroes are used to fill empty positions.
2.1.4.5 Flag fields are numeric fields consisting of a single character.
2.1.4.6 Empty fields
2.1.4.6.1 Text fields which are defined, such as Dr. Name, but
which are empty because the operator did not input
data are filled with the "-" character (2D hex).
2.1.4.6.2 Numeric fields which are defined but which have no
data reported by the Instrument are filled with the "-"
character (2D hex). Example: there is no 5 part diff for
Background counts, so Fields 11 through 15 in the
Count Data message are reported as "-" characters.
2.1.4.7 Spare Fields
2.1.4.7.1 Spare numeric fields (5 characters except for
Sequence Number which is 4 characters) consist
of five (5) zeroes.
2.1.4.7.2 Spare flag fields consist of a single zero.
2.1.4.8 Instrument messages convey results that may be out of range.
Out-of-range numeric fields are filled with the ">" character (3E hex).
2.2 Instrument Messages
Instrument messages are transmitted by the Instrument to the Host. All of these contain
result data. Each message comprises an identification segment followed by a results
segment.
CELL-DYN

03H50-01F May 1999
2.2.1 Instrument Transmission Modes
2.2.1.1 Run Menu Automatic Transmit Mode
The operator may select automatic transmit mode in the Setup Menu.
This mode allows the automatic transmission of results during the RUN
cycle. The operator may also request the Instrument to transmit
histogram data in the automatic transmit mode. If histogram
transmission is not requested, then only the count data is transmitted.
2.2.1.2 Data Log Menu Transmit Mode
On the Data Log Menu, the operator may request transmission of
results from a block of samples. Only count data is transmitted.
2.2.1.3 Display Specimen Menu Transmit Mode
On the Display Specimen Menu, the operator may request
transmission of results from the specimen being displayed. Only count
data is transmitted.
2.2.2 Message Identification Segment Hematology Mode
In the Hematology Mode, the Identification Segment of each message gives the
message type and identifies the specimen.
2.2.2.1 Message Type Field I
The Message Type is the first field in the Identification Segment. It
occupies an alphanumeric field of three (3) characters plus the enclosing
double quotation marks. The seven message types are as follows:
WBC Histogram Message 1: "WB1"
WBC Histogram Message 2: "WB2"
RBC Histogram Message: "RBC"
PLT Histogram Message: "PLT"
WIC Histogram Message: "WIC"
Count Data Message: " " (3 blanks)
Retic Data Message: "RTC"
2.2.2.2 Sequence Number Field 2
The sequence number is the second field in the Identification Segment.
It is automatically generated by the Instrument, and it occupies a
numeric field of four (4) characters.
2.2.2.3 Vet Package On Field 3
Vet Package On is the third field in the Identification Segment. This
field has a 1 if the veterinary package is turned on, or a 0 if it is not.
The meaning of field 6 is a function of the setting of this flag.
4 CELL-DYN

03H50-01F May 1999
2.2.2.4 Specimen Type - Field 4
The specimen type is the fourth field in the Identification Segment. It
occupies a numeric field of one character whose value gives the specimen
type as follows:
0: Patient
1: QC sample
2: Background
3: Electrical background
4: Calibrator (for calibration)
5: Whole-blood calibrator (for calibration)
6: Latex
7: MPV latex (for calibration)
8: Resistant RBC
9: Auxiliary
2.2.2.5 Specimen ID Field 5
The specimen ID is the fifth field in the Identification Segment. For
patient samples, the specimen ID is entered by the operator on the
Instrument, while for other samples, such as controls and
background samples, the specimen ID is supplied by the
Instrument. In either case, it occupies a text field of twelve (12)
characters plus the enclosing double quotation marks.
2.2.2.6 Name (Specimen or Animal) Field 6
The name is the sixth field in the Identification Segment. It occupies a
text field of sixteen (16) characters plus the enclosing double quotation
marks. If the Vet Package is on (field 3) then the name is the name of
the animal. If the Vet Package is off, then the name is the patient name
for sample types of patient, and it is undefined otherwise.
2.2.2.7 Operator ID Field 7
The operator ID is the seventh field in the Identification Segment. It
occupies a text field of three (3) characters plus the enclosing
double quotation marks.
2.2.2.8 Specimen Date Field 8
The specimen date is the eighth field in the Identification Segment. It
is generated by the Instrument when the specimen is run. It occupies
a text field of eight (8) characters plus the enclosing double quotation
marks in a format that depends upon a choice made in the
Date/Time Submenu in the Setup Menu by the operator: month/
day/year, day/month/year, year/month/day, or year/day/month.
CELL-DYN

03H50-01F May 1999
2.2.2.9 Specimen Time Field 9
The specimen time is the final field in the Identification Segment. It is
generated by the Instrument when the specimen is run. It occupies a
text field of five (5) characters plus the enclosing double quotation
marks, and its format is "HH:MM", where HH is the hour (from 00 to 23)
and MM is the minute. The 24-hour method of representing time is used.
2.2.3 Results Segment Hematology Mode
The Results Segment contains numeric data and flags generated by the
Instrument. There are two different Result Segment forms, one for histogram and
one for count data.
2.2.3.1 Results Segment of a Histogram Message
2.2.3.1.1 Scale Factor Field 10
The scale factor is a number that holds the full-range value
for the following normalized histogram data. The scale factor
is a numeric field, five (5) characters in length, with an
implied decimal point after its fourth character. At present,
the scale factor is not implemented, and so it is always
transmitted as five (5) zero characters.
2.2.3.1.2 Channel Data Fields 11 through 74
The channel data fields are normalized counts from every
fourth channel of the 256 channels used internally in the
Instrument. The channel data appear in numeric fields three
(3) characters in length.
2.2.3.1.3 Histogram Message Example
STX [Start of text]
"WB1" [message type]
0416, [sequence no.]
0, [vet package on/off]
0, [specimen type]
"------------" [specimen ID]
"----------------" [specimen name]
"stc", [operator ID]
"01/25/89", [specimen date]
"14:08", [specimen time]
00000, [scale factor]
6 CELL-DYN

03H50-01F May 1999
000, [channel 2 data]
CELL-DYN

03H50-01F May 1999
F7 [checksum]
1
ETX [end of text]
1
The checksum is shown for example only.
8 CELL-DYN

03H50-01F May 1999
2.2.3.2 Results Segment of a Count Data Message
2.2.3.2.1 Conversion of Count Data Fields
Each blood parameter in the Count Data
message (fields 10 through 31) is transmitted in
a numeric field of five (5) characters, one of which
is usually a floating decimal point. The values are
sent in US units. Conversion to other units is the
responsibility of the Host.
The hemoglobin parameters (HGB, MCH, and
MCHC) must also be multiplied by 0.6206 when
modified SI units are being used.
2.2.3.2.2 WBC Count Field 10
2.2.3.2.3 NEU Count Field 11
2.2.3.2.4 LYM Count Field 12
2.2.3.2.5 MONO Count Field 13
2.2.3.2.6 EOS Count Field 14
2.2.3.2.7 BASO Count Field 15
2.2.3.2.8 RBC Count Field 16
2.2.3.2.9 HGB Value Field 17
2.2.3.2.10 HCT Value Field 18
2.2.3.2.11 MCV Value Field 19
2.2.3.2.12 MCH Value Field 20
2.2.3.2.13 MCHC Value Field 21
2.2.3.2.14 RDW Value Field 22
2.2.3.2.15 PLT Count Field 23
2.2.3.2.16 MPV Value Field 24
2.2.3.2.17 PCT Value Field 25
2.2.3.2.18 PDW Value Field 26
2.2.3.2.19 NEU % Value Field 27
CELL-DYN

03H50-01F May 1999
2.2.3.2.20 LYM % Value Field 28
2.2.3.2.21 MONO % Value Field 29
2.2.3.2.22 EOS % Value Field 30
2.2.3.2.23 BASO % Value Field 31
2.2.3.2.24 Moving Average Flag Field 32
The moving-average flag (X bar B) is a
numeric field of one character. A 1 indicates
that the Specimen was included in the X bar
B RBC batch, while a 0 indicates that it was
not included.
2.2.3.2.25 DFLT Flag Field 33
This flag is in a numeric field of one
character. A 1 indicates that the flag is set,
while a 0 indicates that it is not set.
2.2.3.2.26 Blast flag Field 34
2.2.3.2.27 Variant Lym Flag Field 35
character. A 1 indicates that the flag is
set, while a 0 indicates that it is not set.
2.2.32.28 DFLT Flag Neutrophil Qualifier Field 36
2.2.3.2.29 DFLT Flag Eosinophil Qualifier Field 37
2.2.3.2.30 DFLT Flag Lymphocyte Qualifier Field 38
2.2.3.2.31 IG Flag Field 39
2.2.3.2.32 Band flag Field 40
10 CELL-DYN

03H50-01F May 1999
2.2.3.2.33 DFLT Flag Monocyte Qualifier Field 41
This flag is in a numeric field of one character. A 1 indicates
that the flag is set, while a 0 indicates that it is not set.
2.2.3.2.34 DFLT Flag Basophil Qualifier Field 42
2.2.3.2.35 IG/Band Flag Field 43
2.2.3.2.36 FWBC Flag Field 44
2.2.3.2.37 WBC Count Flag Field 45
2.2.3.2.38 Nucleated RBC Flag Field 46
2.2.3.2.39 DLTA Flag Field 47
that there is a WIC WOC delta and that a DLTA flag is set.
A 0 indicates that a WIC WOC delta does not exist and that
the flag is not set.
2.2.3.2.40 NWBC Flag Field 48
2.2.3.2.41 RBC MORPH Flag Field 49
This flag is in a numeric field of one character. A 1
indicates that the flag is set, while a 0 indicates that it is
not set.
2.2.3.2.42 RRBC Flag Field 50
2.2.3.2.43 Spare Flag Field 51
This flag is in a numeric field of one character. Its value is
undefined at this time.
CELL-DYN

03H50-01F May 1999
2.2.3.2.44 Platelet Recount Flag Field 52
This flag is in a numeric field of one character. A 1
indicates that the flag is set, while a 0 indicates that it is
not set.
2.2.3.2.45 Spare Flag Field 53
This flag is in a numeric field of one character. Its value
is undefined at this time.
2.2.3.2.46 LRI Flag Field 54
The LRI flag is a numeric field of one character. A 0
means the flag is not set, and a 1 means that is it set.
2.2.3.2.47 URI Flag Field 55
The URI flag is a numeric field of one character. A 0
means the flag is not set, and a 1 means that it is set.
2.2.3.2.48 RBC Count Time Field 56
The RBC count time is an integer in a five-character
numeric field. It represents the RBC count time in
milliseconds.
2.2.3.2.49 RBC Upper Meniscus Time Field 57
The RBC upper meniscus time is an integer in a five-
character numeric field. It represents the RBC upper
meniscus time in milliseconds.
2.2.3.2.50 RBC Recount Upper Time Field 58
The RBC recount upper time is an integer in a five-
character numeric field. It represents the RBC recount
time in milliseconds.
2.2.3.2.51 RBC Recount Upper Meniscus Time - Field 59
The RBC recount upper meniscus time is an integer in
a five-character numeric field. It represents the RBC
recount upper meniscus time in milliseconds.
2.2.3.2.52 WIC Count Time Field 60
The WIC count time is an integer in a five-character
numeric field. It represents the WIC count time in
milliseconds.
2.2.3.2.53 WIC Upper Meniscus Time Field 61
The WIC upper meniscus time is an integer in a five-
character numeric field. It represents the WIC upper
meniscus time in milliseconds.
12 CELL-DYN

03H50-01F May 1999
2.2.3.2.54 Spare Fields Field 62, 63
Both of these fields are integers in five-character numeric
fields. Their values are undefined at this time.
2.2.3.2.55 WIC WBC Concentration Field 64
The WIC WBC concentration is a numeric field with 5
characters, including a decimal point. It represents the
WIC derived WBC concentration.
2.2.3.2.56 WOC WBC Concentration Field 65
The WOC WBC concentration is a numeric field with 5
characters, including a decimal point. It represents the
WOC derived WBC concentration.
2.2.3.2.57 Limits Set Field 66
The limits set is a numeric field of one character. It
indicates which set of patient limits (1 to 4) was in effect
for the given patient specimen.
2.2.3.2.58 Sample Mode Field 67
The sample mode is a numeric field of one character. A
0 indicates that the specimen was taken in Open Sample
Mode; a 1 indicates Closed Sample Mode.
2.2.3.2.59 RBC Metering Fault Flag Field 68
The RBC metering fault flag is a numeric field of one
character. A 0 indicates that there was no metering
fault, a 1 indicates a flow error, and a 2 indicates a
clog error.
2.2.3.2.60 WIC Metering Fault Flag Field 69
The WIC metering fault flag is a numeric field of one
character. A 0 indicates that there was no metering
fault, a 1 indicates a flow error, and a 2 indicates a
clog error.
2.2.3.2.61 Sampling Error/Incomplete Aspiration Flag Field 70
This is a numeric field of one character. A 0 indicates
that there was no sampling error, while a 1 indicates
that there was such an error.
CELL-DYN

03H50-01F May 1999
2.2.3.2.62 Count Data Message Example
STX [Start of text]
" ", [message type]
0, [Vet package on/off]
0, [specimen type]
"------------", [specimen ID]
"-----------------", [specimen name]
05.00, [WBC count]
03.20, [NEU count]
01.20, [LYM count]
0.300, [MONO count]
0.200, [EOS count]
0.100, [BASO count]
04.94, [RBC count]
015.3, [HGB value]
043.1, [HCT value]
092.3, [MCV value]
031.0, [MCH value]
034.4, [MCHC value]
012.0, [RDW value]
00177, [PLT count]
010.7, [MPV value]
01.90, [PCT value]
019.2, [PDW value]
064.0, [NEU % value]
024.0, [LYM % value]
06.00, [MONO % value]
04.00, [EOS % value]
14 CELL-DYN

03H50-01F May 1999
02.00, [BASO % value]
1, [moving average flag]
1, [DFLT flag]
0, [Blast flag]
0, [Variant Lymph flag]
1, [DFLT Flag Neutrophil Qualifier]
1, [DFLT Flag Eosinophil Qualifier]
0, [DFLT Flag Lymphocyte Qualifier]
0, [IG flag]
0, [Band flag]
0, [DFLT Flag Monocyte Qualifier]
0, [DFLT Flag Basophil Qualifier]
0, [IG/Bands flag]
0, [FWBC flag]
1, [WBC count flag]
0, [Nucleated RBC flag]
0, [DLTA flag]
0, [NWBC flag]
1, [RBC morphology flag]
0, [RRBC flag]
0, [spare flag]
0, [Platelet Recount flag]
0, [spare flag]
1, [LRI flag]
0, [URI flag]
07761, [RBC count time]
02473, [RBC upper meniscus time]
00000, [RBC Recount time]
00000, [RBC Recount upper meniscus time]
02445, [WIC count time]
01223, [WIC upper meniscus time]
00000, [spare field]
00000, [spare field]
CELL-DYN

03H50-01F May 1999
05.00, [WIC WBC concentration]
04.80, [WOC WBC concentration]
3, [limits set]
1, [sample mode]
0, [RBC metering fault flag]
1, [WIC metering fault flag]
0, [Sampling Error/incomplete aspiration flag]
F7 [checksum]
1
ETX [end of text]
1
2.2.4 Message ldentification Segment ReticMode
This section applies only to Reticulocyte samples. The Identification Segment of
each message gives the message type and identifies the specimen.
2.2.4.1 Message Type Field 1
The Message Type is the first field in the Identification Segment. It
occupies an alphanumeric field of three (3) characters plus the
enclosing double quotation marks as follows:
Retic Data Message: "RTC"
2.2.4.2 Sequence Number Field 2
The Sequence Number is the second field in the Identification Segment.
It is automatically generated by the Instrument, and it occupies a
numeric field of four (4) characters.
2.2.4.3 Vet Package On Field 3
Vet Package On is the third field in the Identification Segment. It
occupies a numeric field of one (1) character. This field has a 1 if the
Veterinary Package is turned on, or a 0 if it is not. At this time, Vet
Package is not available for the Retic mode.
2.2.4.4 Specimen Type Field 4
The Specimen Type is the fourth field in the Identification Segment. It
occupies a numeric field of one character whose value gives the
specimen type as follows:
0: Patient type
1: QC sample type
2: Background type
16 CELL-DYN

03H50-01F May 1999
The Specimen ID is the fifth field in the Identification Segment. For
patient samples, the Specimen ID is entered by the operator on the
Instrument, while for other samples, such as controls and
background samples, the Specimen ID is supplied by the
Instrument. In either case, it occupies a text field of twelve (12)
characters plus the enclosing double quotation marks.
2.2.4.6 Specimen Name Field 6
The Specimen Name is the sixth field in the Identification Segment.
It occupies a text field of sixteen (16) characters plus the enclosing
double quotation marks. It is undefined except for patient samples.
2.2.4.7 Specimen Sex Field 7
The Specimen Sex is the seventh field in the Identification Segment.
It consists of one (1) character "M" or "F" plus the enclosing double
quotation marks. If not entered by the operator, Specimen Sex is
transmitted as a "-" character (2D hex) plus the enclosing double
quotation marks.
2.2.4.8 Specimen Date of Birth (DOB) Field 8
The Specimen Date of Birth is the eighth field in the Identification
Segment. It occupies a text field of eight (8) characters plus the
enclosing double quotation marks in a format that depends on a
choice made in the Date/Time Submenu in the Setup Menu by the
operator: month/day/year, day/month/year, year/month/day, or
year/day/month.
2.2.4.9 Dr. Name Field 9
The Dr. Name is the ninth field in the Identification Segment. It
occupies a text field of twenty two (22) characters plus the enclosing
2.2.4.10 Operator ID Field 10
The Operator ID is the tenth field in the Identification Segment. It
occupies a text field of three (3) characters plus the enclosing
2.2.4.11 Specimen Date Field 11
The Specimen Date is the eleventh field in the Identification Segment. It
is generated by the Instrument when the specimen is run. It occupies a
text field of eight (8) characters plus the enclosing double quotation
marks in a format that depends on a choice made in the Date/Time
Submenu in the Setup Menu by the operator: month/ day/year,
day/month/year, year/month/day, or year/day/month.
CELL-DYN

03H50-01F May 1999
2.2.4.12 Specimen Time Field 12
The Specimen Time is the final field in the Identification Segment. It is
generated by the Instrument when the specimen is run. It occupies a
text field of five (5) characters plus the enclosing double quotation
marks, and its format is "HH:MM", where HH is the hour (from 00 to 23)
and MM is the minute. The 24-hour method of representing time is used.
2.2.5 Results Segment Retic Mode
2.2.5.1 Count Data Message
Each parameter in the Count Data message (fields 13 through 16) is
transmitted in a text field of four (4) digits plus a floating decimal point
and enclosing double quotation marks (a total of 7 characters). Fields 17
through 22, which are also text fields, are spare fields.
Retic% Field 13
Retic Abs Field 14
RBC Count Field 15
Background Count Field 16
Note: If the Specimen Type is Background, Field 16 will contain the
background count. Otherwise, Field 16 will contain "0.000".
* Immature Reticulocyte Fraction Field 17
Spare Field 18
Spare Field 19
Spare Field 20
Spare Field 21
Spare Field 22
* Confirmation not available.
2.2.5.2 Retic Flags
2.2.5.2.1 Retic Flow Error Flag Field 23
This flag is in a numeric field of one (1) character. A 1 indicates a
flow error, while a 0 indicates no flow error.
2.2.5.2.2 Fragile RBCs Flag Field 24
This flag is in a numeric field of one (1) character. A 1 indicates the
presence of fragile RBCs, while a 0 indicates no fragile RBCs.
18 CELL-DYN

03H50-01F May 1999
2.2.5.2.3 Too Few Events Flag Field 25
This flag is in a numeric field of one (1) character. A 1 indicates too
few cells, while a 0 indicates sufficient cells are present.
2.2.5.2.4 Excessive Nucleated Cells (ENC) Flag Field 26
This flag is in a numeric field of one (1) character. A 1 indicates an
ENC error, while a 0 indicates no ENC error.
2.2.5.2.5 Excessive RBC Loss (ERL) Flag Field 27
This flag is in a numeric field of one (1) character. A 1 indicates an
ERL error, while a 0 indicates no ERL error.
2.2.5.2.6 No Hematology Data Warning Flag Field 28
This flag is in a numeric field of one (1) character. A 1 indicates no
hematology data was found, while a 0 indicates hematology was
found.
2.2.5.2.7 Spare Fields
Fields 29 through 32 are spare fields. Each consists of a numeric
field of one (1) character.
Field 29 0 [Spare Flag]
2.2.5.3 Retic Data Message Example
STX [Start of text]
"RTC", [message type]
0, [Vet package on/off]
0, [specimen type]
"------------", [specimen ID]
"----------------", [specimen name]
"M", [specimen sex]
"10/25/75", [specimen DOB]
"----------------------" [Dr name]
CELL-DYN

03H50-01F May 1999
"1.000", [Retic%]
"50.00", [Retic Abs]
"5.000", [RBC count]
"0.000", [Background count]
"0.010", [Immature Reticulocyte Fraction]
"0.000", [spare field]
1, [Flow error]
0, [Fragile RBCs]
0, [Too Few Events]
0, [ENC]
0, [ERL]
0, [No hematology data]
0, [spare flag]
0, [spare flag]
0, [spare flag]
0, [spare flag]
F7 [checksum]
1
ETX [end of text]
1
2.3 Host Messages
Host messages are transmitted by the Host to the Instrument. The Instrument uses Host
messages to add entries to the work list. However, if a Host message contains syntax or
functional errors, the work list is not altered.
2.3.1 Instrument Reception Mode
To prepare the Instrument to receive work list messages from the Host, the
operator should turn the bar code option on in the Work List menu, then go to the
Work List Set Up menu and choose the desired association for the bar code ID
(4-digit CELL-DYN 3500 System bar code or Laboratory Specimen ID) When the
bar code option is off, the Instrument considers all Host messages to contain
syntax errors.
20 CELL-DYN

03H50-01F May 1999
2.3.2 Host Message Format
2.3.2.1 Four-Digit Bar Code Field 1
This is a text field of four characters plus enclosing quotation marks,
giving the four-digit bar code to be used for the given specimen, if
any. The format requirements for this field are as follows: if the work
list is set up on the Instrument to use four-digit bar codes, this field
must be filled with characters which are digits; if four-digit bar codes
are not being used, this field must be filled with either the "-"
character or digits (the value is ignored).
This is a text field of twelve characters plus enclosing quotation
marks, giving the specimen ID to be used for the given specimen, if
any. If four-digit bar codes are being used, this field may be filled with
the "-" character to signify that no other specimen ID is required.
Otherwise, if this field is filled with the "-" character or is blank, the
Instrument will conclude that the message contains a syntax error.
2.3.2.3 Specimen Name Field 3
This is a text field of sixteen characters plus enclosing quotation
marks, giving the specimen name to be used for the given
specimen, if any. If there is no specimen name, this field should be
filled with the "-" character.
2.3.2.4 Limit Set Field 4
This is a numeric field of one character, giving the limit set to be used
for the given specimen. Valid values range from 0 to 4, with 0 signifying
that the Instrument should use the default limit set value as specified
on the Work List Set Up menu. If this field does not contain a valid
value, the Instrument will conclude that the message contains a syntax
error.
2.3.2.5 Parameter Set Field 5
This is a numeric field of one character, giving the parameter set to
be used for the given specimen. Valid values range from 0 to 4, with
0 signifying that the Instrument should use the default parameter set
value as specified on the Work List Set Up menu. If this field does
not contain a valid value, the Instrument will conclude that the
message contains a syntax error.
CELL-DYN

03H50-01F May 1999
2.3.2.6 Doctor Name Field 6
This is an optional field that, if used, must be followed by the Date of
Birth field, otherwise the message will be rejected. This is a text field of
22 characters plus enclosing quotation marks, giving the name of the
patients Doctor for the given specimen. If there is no Doctor Name,
this field should be filled with the "-" character, however, any printable
character string shall be accepted.
2.3.2.7 Date of Birth Field 7
This is an optional field that, if used, must be preceded by the Doctor
Name field, otherwise the message will be rejected. This is a text field of
8 characters of form mm/dd/yy plus enclosing quotation marks, giving the
patients date of birth for the given specimen. If there is no Date of Birth,
this field must be represented as --/--/--, otherwise the Instrument will
conclude that the message contains a syntax error.
2.3.3 Short Entry Work List Message Example
STX [Start of text]
"1135", [four-digit bar code]
"237-45-7789", [specimen ID]
"Smith, Richard ", [specimen Name]
0, [limit set]
3, [parameter set]
F7 [checksum]
1
ETX [end of text]
1
2.3.4 Long Entry Work List Message Example
STX [Start of text]
"1135", [four-digit bar code]
"237-45-7789," [specimen ID]
"Smith, Richard ", [specimen Name]
0, [limit set]
3, [parameter set]
"Jones, Henry ", [doctor name]
"09/30/45", [date of birth]
F7 [checksum]
1
ETX [end of text]
1
22 CELL-DYN

03H50-01F May 1999
3.0 RS-232 TRANSPORT
3.1 Mechanical Interface
The Instrument provides a standard DB9 male connector mounted on the back
panel. The RS-232C interface is configured as DTE (Data Terminal Equipment). A
cable is available for converting the 9-pin interface to a standard 25-pin interface.
Pins on the DB9 connector:
Pin 1: Carrier Detect (CD), an input (not used)
Pin 2: Receive Data (RxD), an input
Pin 3: Transmit Data (TxD), an output
Pin 4: Data Terminal Ready (DTR), an output
Pin 5: Signal Ground
Pin 6: Data Set Ready (DSR), an input
Pin 7: Request to Send (RTS), an output
Pin 8: Clear to Send (CTS), an input
Pin 9: Ring Indicator (RI), an input (not used)
Further information on the physical interface can be obtained from the IBM Technical
Reference, Options and Adapters (Copyright IBM Corporation).
3.2 Electrical Interface
Voltage levels and electrical characteristics are defined by the EIA RS-232C
specification. The maximum recommended cable length is 30 meters, or 100 feet.
The maximum workable cable length may differ from that figure because of
environmental factors, the selected baud rate, and the nature of the equipment being
connected.
3.3 Data Link
3.3.1 Symmetrical Interface
The communication is full duplex; the Host and the Instrument may begin
transmitting at any time except as restricted by error control and recovery.
3.3.2 Asynchronous Serial
The transmission of data by the Instrument is in an asynchronous, bit-serial
format, using the ASCII character set. A setup menu is provided for specifying
the number of bits per character (seven or eight), the number of stop bits (one
or two), the parity (even, odd, or none), and the baud rate (300, 600, 1200,
2400, 4800, or 9600).
CELL-DYN

03H50-01F May 1999
3.3.3 Message Frame
Each data message (defined in section 2) is transported in a frame composed
of the start character, data message, checksum, and the stop character. All
characters are printable ASCII values except the start and stop, which have the
default values two and three, respectively. The ASCII standard defines these
as STX and ETX. The operator can choose alternative unprintable characters,
but this document uses "STX" and "ETX" as synonyms for the start and stop
characters, whatever their values.
The checksum is provided for the receiver to verify message integrity. It is
generated by taking the modulo 256 sum of the characters in the data
message. The checksum includes all characters in the message except itself,
the STX, and the ETX. The two-digit ASCII-hexadecimal representation of the
checksum is placed immediately before the ETX.
3.3.4 Error Recovery Response From Host
3.3.4.1 Between-Transmission Time-Out
The time-out interval after transmission of one message is programmable
from 0.1 second to 9.9 seconds in increments of 0.1 second.
3.3.4.2 Release for Next Message
The Host can release the Instrument to send the next message by sending
an ACK (06 hex) before the time-out interval passes. Otherwise, the
Instrument will send the next message automatically if the selected time-out
interval passes.
3.3.4.3 Retransmission
If for any reason the Host requires retransmission of the message, it signals
the Instrument by sending a NAK (15 hex) before expiration of the time-out
interval. A message will be sent by the Instrument a maximum of twice.
After this, the same conditions prevail as after a time-out.
3.3.5 Response to Host
The Host should wait for one of these three responses before it sends another
message to the Instrument.
3.3.5.1 NAK Response
If the Instrument receives a message that fails its test for integrity (see
section 3.3.3), it sends a NAK (15 hex) character to signal the Host
that it should transmit the message again.
24 CELL-DYN

03H50-01F May 1999
3.3.5.2 DEL Response
If the Instrument receives a message that passes its test for
integrity but has an incorrect format (see section 2.3), then it
sends a DEL (7F hex) character to reject the message.
integrity but the bar code identification (4-digit specimen ID, as
chosen by the operator) matches an entry already in the work list,
then it sends a DEL character to reject the message.
3.3.5.3 CAN Response
If the Instrument receives a message that passes the integrity test
but the bar code option is off or the work list is full, it sends a CAN
(18 hex) character to reject the message.
3.3.5.4 ACK Response
integrity and does not need to be rejected for the reasons cited in
sections 3.3.5.1 through 3.3.5.3, it sends an ACK (06 hex)
character to signal the Host that it is ready to receive the next
message.
3.3.6 XON/XOFF and CTS
Two methods of transmission control are provided: XON/XOFF and CTS
(hardware handshake). To use XON/XOFF control, the Host must transmit an
XOFF character (13 hex) to the Instrument while the Host still has room for at
least two additional characters from the Instrument and must transmit an XON
character (11 hex) to cause the Instrument to resume transmission. To use
CTS control, the Host must set the CTS signal false while the Host still has
room for at least two additional characters and must set CTS true again to
cause transmission to resume. There is a two-second time-out for XON/XOFF
control. Transmission will automatically resume two seconds after an XON/
XOFF character is received even if no XON character follows it. There is no
time-out with CTS contol.

Cell Dyn 3500

Hochgeladen von

Dokumentinformationen

Originalbeschreibung:

Copyright

Verfügbare Formate

Dieses Dokument teilen

Dokument teilen oder einbetten

Freigabeoptionen

Stufen Sie dieses Dokument als nützlich ein?

Sind diese Inhalte unangemessen?

Copyright:

Verfügbare Formate

Cell Dyn 3500

Hochgeladen von

Copyright:

Verfügbare Formate

HOST INTERFACE SPECIFICATION

3500 System Host Interface Specification with Reticulocyte Option i

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 1

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 3

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 5

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 7

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 9

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 11

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 13

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 15

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 17

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 19

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 21

3500 System Host Interface Specification with Reticulocyte Option

3500 System Host Interface Specification with Reticulocyte Option 23

3500 System Host Interface Specification with Reticulocyte Option

Das könnte Ihnen auch gefallen