About the Animal Biotechnology Centre, Nanaji Deshmukh Veterinary

Science University, Jabalpur

Opportunity of admission in M.V.Sc with DBT-JRF fellowship


Nanaji Deshmukh Veterinary Science University, Jabalpur
No.525DI/NDVSU/2014 Dated: 08/08/2014
ADMISSION NOTICE
Application on the prescribed form are invited for admission
through All India Merit Basis to M.V.Sc. (10 seats) and Ph.D. (02
seats) in Animal Biotechnology Centre, NDVSU, Jabalpur, under
Department of Biotechnology-HRD, GoI, New Delhi supported
programme for the academic session 2014-15. Each student admitted
in M.V.Sc. programme will be paid the DBT-JRF fellowship of Rs.
8000/- PM. The last date for submission of form is 29/08/2014 and
counselling will be on 30/08/2014 The details can be downloaded
from website : www.mppcvv.org.
Director Instructions


Highlights of Animal Biotechnology Centre

Biotechnology centre, Jabalpur, was established by financial assistance of Madhya
Pradesh State Agriculture Cooperative Board (Mandi Cess Fund), Bhopal, with the
Budget outlay worth of Rs. 7.43 crore in the year 2002-03. Centre is well equipped
with most modern equipments like Next Generation Sequencer (ABI), Capillary
Sequencer, Flowcytometer with cell sorter (BD), Real Time PCR (ABI),
Micromanipulating system (Nikon Narishge), Trinocular Fluorescent Microscope
(Leica) etc. The centre has highly qualified, experienced faculty, well trained in
modern area of molecular and embryo biotechnology.





Biotechnology Building
Objectives of the Centre

1. Human resource development in Animal Biotechnology.
2. Characterization and conservation of Animal Genetic resource using reproductive and
molecular techniques.
3. Diagnosis of animal diseases using molecular and ELISA base techniques.
4. Embryo Biotechnology for livestock improvement and conservation through IVF and
ETT.
5. Development of clone and transgenic animal for improving the livestock productivity.

Achievement of Animal Biotechnology Centre

Research Projects Undertaken:

Till date the centre has successfully completed 6 research projects funded by DBT,
ICAR-NAIP, RKVY etc. The centre has successfully completed two NAIP research projects
out of which for one project entitled Development of goat having knocked down myostatin
gene through RNA interference technology to enhance the meat production, the centre was
recognized as National Lead Centre along with two partners, one from Department of
Genetics, IVRI, Bareilly and other from Department of Animal Biotechnology, AAU, Anand,
Gujrat. Under this project twenty research papers has been published, mostly in International
Journals having high impact factor. One patent entitled Development of cost effective
microtools for oocyte enucleation during SCNT embryo production has been submitted vide
patent file No. 1762/MUM/2012 dt. 19-06-2012 which is the first patent filed in our
university.

Other NAIP project entitled Developmental Potency of Parthenogenetic Goat
Embryos, the centre was selected as consortium partner of IVRI. These two projects were
based on most advanced molecular and reproductive bio-techniques like RNAi for down
regulating myostatin gene and production of stably transfected cell line having knocked down
myostatin gene, Microarray for embryonic developmental genes, production of cloned
embryos by using two innovative techniques (partial replenishment of ooplasm and use of
patented cost effective micro-tools), embryonic gene expression techniques in
parthenogenetic, IVF and cloned embryos etc.

Placement

Till date more than 55 M.V.Sc. students have passed out from this centre and 11 of
them have been selected for Ph.D. degree in various premier National Institute of India like
Centre for Cellular and Molecular Biology, Hyderabad, Rajiv Gandhi Centre for
Biotechnology, Thiruvananthapuram, Indian Veterinary Research Institute, Izatnagar,
National Dairy Research Institute, Karnal etc. and more than 95% students have been well
placed in different government and private industries with good position.
FUTURE PROSPECTS:

Publications of research: Students mostly published research papers from their theses work
in peer reviewed international journals with high impact factor which will help in getting
placement in good national and international institute.

Embryo Biotechnology division of this centre is doing exceeding well during last 5-6 years
and many publications have appeared in International Journals. Thus students well trained in
embryo production and micromanipulation can easily be absorbed by human IVF
laboratories. There are hundreds of IVF laboratories in India and their number is increasing
day by day. At present in Madhya Pradesh, which was considered as backward state has more
than 10 such laboratories and more than 25 in Central India. Our students are also getting
good offers from these laboratories (around average 5-7 lakhs at entry level and upto a
package of 20 lakhs within 5-6 years of experience). Hence, our PG students have option to
enter in human IVF areas at very good salary with bright future prospects.

Advanced technology based research: The students are getting exhaustive training in
molecular techniques related to DNA polymorphism, gene expression, sequencing using
automated DNA Sequencer, RNAi and cell culture. Shortly students will get opportunity to
work on next generation sequencer for more advanced sequencing. These students, well
trained in molecular technique can get placement in different pharmaceutical and
biotechnology industries in R&D section.

Transgensis and cloning: Students also get the opportunity to work on transgenesis and
cloning techniques which may help in getting placement abroad for Ph.D. and post doc
research.

Stem cell Research - We have already initiated work on stem cell techniques and in coming
days stem cell research will be intensified hence opening many new avenues of research and
placement in such advance technology based industries.

Hence as director of this centre, I can assure that the students will get full opportunity
to work in any area of research as per their choice and will get free hand to utilize the most
modern and expensive equipments.

Photographs showing highlights of research work done in the Centre






Ooplasm Replishment
technology for SCNT
embryo production






The process of
enucleation by
patented technology
and subsequent
production of excellent
blastocyst













Different developmental
stages of IVF embryos
along with Hoechst
stained Blastocyst nuclei









Transfection and transient expression of myostatin gene in goat fibroblast and myoblast cell line
Important publications of the Centre during the Last Five Years:

1. Menka Sharma, Sanjeev Singh, Ameeta Kushwah and B.C. Sarkhel (2009). Molecular
characterization of Malvi and Sahiwal breeds of cattle (Bos indicus) by RAPD-PCR.
Indian Journal of Animal Science. 79 (1): 44-46.
2. G. S. Ajithkumar, B. Krishnamohan and B.C. Sarkhel (2009) Optimization of
enucleation time after ivm for somatic cell nuclear transfer in goat, Reproduction,
Fertility and Development, Vol. 21(1), pp. 109-110
3. Sanjeev Singh, Pushpendra Kumar, T K Bhattacharya, Bharat Bhushan, and Arjuva
Sharma. PCR-SSCP and nucleotide sequencing of Kappa casein gene in Indian
buffalo. The Indian Veterinary Journal, 86(6):592-593, June 2009.
4. Monika Koul, Megha Kadam, YPS Malik, AK Tiwari, JL Vegad and B.C. Sarkhel
(2009), Nucleocapsid gene sequence anlysis and characterization of an Indian isolate
of Avian infectious bronchitis virus. International journal of poultry Science. 8 (1)
5. Hemlata Jain, Sanjeev Singh, Megha Kadam, and B.C. Sarkhel (2010) Knockdown
of the myostatin gene by RNA interference in caprine fibroblast cells, Journal of
Biotechnology (Elseviar), 145 (2010) 99102.
6. Sanjeev Singh, Suneet Sharma, J.S. Arora and B.C. Sarkhel. (2010) Molecular and
biochemical evaluation of Indian breeds of cattle (Bos indicus). Biochemical Genetics
2010 DOI 10.1007/s10528-010-9402-8.

7. Shiva Nema, Sanjeev Singh, Megha Kadam and B.C. Sarkhel. Identification of Kappa
casein alleles by PCR-SSCP. The Indian Veterinary Journal, 87(4):334-336, April
2010.

8. Koul, M., Jain, S.K., Singh, S. and Bedekar, M.K. (2011). Nucleocapsid Gene
Sequence Analysis and Characterization of Indian Isolates of Avian Infectious
Bronchitis Virus. International Journal of Advanced Biotechnology Research 1:85-91.
9. Arvind Sharma, S. N. Shukla, R. G. Agrawal, E. Joseph, B C Sarkhel (2011) Fertility
response on administration of insulin in repeat breeding crossbred cows Short
communication, Indian Journal of Animal Sci. Vol.81 (2).
10. Singh, Nirmal, Singh, Sanjeev; Jain, Sudhir and Sarkhel, Bikash (2012) Evaluation of
interferon response induced by anti-myostatin shRNA constructs in goat (Capra
hircus) fetal fibroblasts by quantitative Real time-Polymerase Chain Reaction,
Animal Biotechnology, 23 (3), 174-183.
11. Azad Singh, Megha Kadam Bedekar, Rakesh Sharma, Bikash Chandra Sarkhel,
Sanjeev Singh and Sudhir Kumar Jain (2012). Detection of very virulent infectious
bursal disease virus from a field outbreak in Central India, Acta Veterinaria

Hungarica, 60 (1), pp. 165174.

12. Tripti Jain, Kadam Megha, Sarkhel B.C. (2013) Prokaryotic expression of N gene of
Infectious Bronchitis virus and assessment of its immunogenicity and antigenicity.
Indian Journal of Animal Research.47 (4): 292-300.
13. Tripti Jain, Meena Haldkar, Bikas C Sarkhel. (2013). Expression profile of BMPRIB

gene in goat reproductive tract. Indian J. Anim. Res., 48: 329 335.


14. Kumar D., Gopalkrishna R., Ajay P. Singh, Rakesh Ranjan, Saurabh K. Pandey and
Bikash C. Sarkhel (2014). Developmental potency of pre-implant parthenogenetic
goat embryos- effect of activation protocols and culture media. In Vitro Cellular and
Developmental Biology- Animal 50(I):1-6. (NAAS 7.5 (NAAS Rating - 7.5).
15. Gopalkrishna R, Kumar D, Singh A P, Pandey S K, Ranjan R, Sarkhel B C 2014.
"Assessment of Developmental Potential of Caprine C loned Embryos with Ooplasm
Replenishment under Two C ulture Media," In Vitro Cell.Dev.Biol.Animal.
(Accepted-DOI: 10.1007/s11626-014-9744-3) (NAAS 7.5)
16. Amol Ashok Sahare, Megha Kadam Bedekar, Sudhir Kumar Jain, Azad Singh,
Sanjeev Singh, Bikas Chandra Sarkhel (2014) Inhibition of infectious bursal disease
virus by vector delivered siRNA in cell culture, Animal Biotechnology (Accepted)

17. Tripathi S.M., Sahani Y.P.S., Sharma R.K., Sankhala L.N., Prem Kumar G., Bedekar
M.K., Sarkhel B.C., Quadri M.A., Thaker A.M. (2014) The effect of subchronic doses
of organophosphorus pesticide , acephate, on hematological and biochemical
parameters of chickens. Veterinary Practitioner (Accepted).


For further details of admission please visit the website : www.mppcvv.org.