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Enzymes in Action
Abstract
The labs purpose is to teach you how to measure the effects of changes in temperature, enzyme
concentration and substrate concentration and how these affect the reactions of enzymes in a
controlled experiment. Enzymes are in our bodies and this is being shown in the experiment
with the liver reacting in the hydrogen peroxide. The methods in this experiment must be
followed as directed. The results show what the hypothesis had stated. It is supporting the
hypothesis by showing that the factors used in the lab are affecting the enzyme-substrate
reaction. This lab is teaching you that enzymes are in living things even in humans. It can
denature by temperature, pH, and enzyme and substrate concentration. Time also has a great
Introduction
Enzymes are proteins that are produced by living cell that act as biochemical catalysts. As
everybody knows an enzyme must bind to a substrate at the active site in order to make a product
of the reaction formed. There are several ways an enzyme action can be affected. Some of the
factors are salt concentration, pH, temperature, and if there are any activations or inhibitors
acting on the enzyme. Many of these factors can make an enzyme denature or slow down making
the reaction inactive. The purpose of this lab is to seeing how enzyme catalysis works. If you put
[Type text] [Type text] Lizet Enriquez B3 Biology Ap
the catalase solution in different things such as hydrogen peroxide mixed with water then you are
For the first test you will need: Hydrogen peroxide, 3 medicine cups, catalase solution, 1 test
tube, test tube rack, cube of liver, 12mL syringe, 1 mL pipette, pencil and paper. For the second
test you will need: Hydrogen peroxide, 8 medicine cups, 6mL syringe, 1 mL pipette, 1 mL of
distilled water in medicine cups, Sulfuric acid, 12mL syringe, and Potassium permanganate.
3. After observing the reaction and recording the results clean all materials and dry
them
2. After that place the test tube in the water bath for 5 minutes
4. Take 1 mL of the cooled boiled catalase solution and add it to the medicine cup
5. After recording the results clean all materials and dry them
3. After recording the results clean all materials and dry them
5. Remove a 5 mL sample
8. Using the syringe add 5 mL of potassium permanganate one drop at a time after each
drop gently swirl and keep adding drops until you get a pink or brown color in the
solution.
Results
bubbles
For the test of catalase activity 2 the solution didn’t bubble as much as activity 1 because the
boiling denatures the catalase. The test of catalase activity 2 it is bubbling more than activity 1 or
[Type text] [Type text] Lizet Enriquez B3 Biology Ap
2 because the liver has catalase. If you would have boiled the liver it would had denatured the
Conclusion
You will see different reactions depending on what you put the enzyme and substrate through.
[Type text] [Type text] Lizet Enriquez B3 Biology Ap
This is proved in the lab when you boil the catalase and you don’t and the one that is not boiled
is more reactive than the one that was boiled. This is showing how temperature affects an
enzyme. Many things you do in the experiment can affect the outcome. Some of these factors are
when you put the drops in the solution you must make sure you are stirring gently, you must
wash the materials correctly in order to avoid left over solution affecting the outcome of the
experiment.