embryo differentiation of neuroblasts was the same as was in FGF-

treated.
Conclusions: Our model system is simpler than the embryo in vivo,
but closer to normal development than the cell cultures used in other
studies. The neuroblasts found were direct answer to molecular
signals added. This fact is in accordance with the idea that in a multi-
level process of neural differentiation, FGF is the neural inducer in
mammals.
doi:10.1016/j.jns.2013.07.2388
Abstract WCN 2013
No: 2576
Topic: 36 Other Topic
Spinal schistosomiasis: Relevance of epidemiologic and imaging
features in an unusual case of myelopathy
J. Morgado
a
, S. Dias
a
, R. Figueira
b
, B. Maia
a
, H. guas
a
, R. Pedrosa
a
.
a
Neurology Department, Centro Hospitalar Lisboa Central, Lisbon, Portugal;
b
Physical Medicine and Rehabilitation Department, Centro Hospitalar
Lisboa Central, Lisbon, Portugal
Background: Neuroschistosomiasis is a common cause of myelopathy
in endemic regions but it is infrequent in developed countries.
Objective: Our goal is to present a case of spinal schistosomiasis
where epidemiologic and imaging features played an important role.
Patients and methods: Clinical case.
Results: A 26-year-old woman from Guinea-Conakry was referred to a
Portuguese Neurosurgery Department for progressive paraparesis and
urinary retention. Spinal MRI showed an intramedullary lesion causing
enlargement of the spinal cord from D910 to L1L2. The lesion was
hyperintense on T2-weighted images and showed a heterogeneous
pattern of contrast enhancement. The patient improved to some extent
without treatment and two weeks later she was transferred to our
Neurology Department. Control MRI revealed slight decrease of the
spinal enlargement and CSF analysis showed lymphocytic pleocytosis
and elevated protein. Brain MRI, somatosensory evoked potentials of
the lower limbs, and blood analysis including cianocobalamine levels
and autoimmunity were normal, and infectious serologies were
negative except for schistosomiasis serology which came back positive.
A diagnosis of spinal schistosomiasis was established based on
epidemiologic and imaging features, supported by a positive schistoso-
miasis serology andexclusionof other causes. Treatment consisted of an
antihelminthic and corticotherapy according to guidelines, as well as
physical rehabilitation. On reevaluation 7 months later there was
clinical improvement and imagiological resolution.
Conclusion: Spinal schistosomiasis is rarely encountered in developed
countries, making diagnosis challenging. Epidemiologic and imaging
features are essential for diagnosis, leading to prompt treatment and
improved prognosis.
doi:10.1016/j.jns.2013.07.2389
Abstract WCN 2013
No: 2589
Topic: 36 Other Topic
Behavior and reproduction of mothers as well as behavior of the
second generation pups are inuenced by maternal separation
R. Bousalham, A. Mesoui. Ibn Tofail University, Kenitra, Morocco
Background: Daily separation of rat pups from their mothers while in
the preweaning period has an impact on behavior and stress response
of pups.
Objective: We hypothesized that maternal separation may create a
depression like state in mother rats from which we separate the rat
pups and decrease their reproductive function and fertility. Also, we
aimed to evaluate the transmission degree of depression and anxiety
across generations.
Material and methods: Females aged 3 months were divided in two
groups of 10 rats each. After a rst mating and a rst parturition, we
conducted maternal separation 3 h during 22 days for the experienced
group while the control group mothers kept their pups. At 4 months of
age, females underwent behavioral tests and a second mating. Second
generation pups were also subjected to behavioral tests.
Results: Behavioral tests shown that mothers who experienced
separation were more depressive and anxious than control ones, also
they have a lower litter size. Rat pups of the second generation whose
mothers' experienced maternal separation also revealed behavior
changes akin to depression and anxiety but less noticeable than the rst
generation pups.
Conclusion: Thus, maternal separation causes depressive and
anxious like-states on mother rats which experienced separation
and has an impact on their litter size. Also, consequences of maternal
separation seem to last throughout generations.
doi:10.1016/j.jns.2013.07.2390
Abstract WCN 2013
No: 2591
Topic: 36 Other Topic
Work stress in health care workers in Hopital El Idrissi
M. Bakrim, R. Bousalham, A. Mesoui. Ibn Tofail University,
Kenitra, Morocco
Background: Health care workers are strongly exposed to work
stress, their conditions of work and multiple other factors increase
their susceptibility to this adjustment disorder. Our work is an
epidemiological study designed to assess the state of total health, to
identify risk factors of stress, its consequences among hospital staff
in of the region of Gharb Cherarda Beni Hsen (Morocco).
Methods: This study is a survey based on a questionnaire covering the
Total Health Test (TST) and the Global Stress Index (GSI), distributed to
doctors and nurses of the CHR and collected for statistical analysis.
Results: Our results have shown the involvement of socio-demo-
graphic factors relating to professional careers in the initiation and
maintenance of stress. Our study showed the dissatisfaction of health
workers, particularly females, towards working conditions. More-
over, a majority of respondents, regardless of their prole, have high
TST scores reecting an alarming exposure to stressful factors.
Conclusion: Aware of the scarcity of works dealing with stress of
health care staff in morocco and negative impact that this stress has
in terms of morbidity and mortality, our work aims to improve the
quality of hospital work in the Gharb region and this by under-
standing and reducing risk factor of this disease.
doi:10.1016/j.jns.2013.07.2391
Abstract WCN 2013
No: 2193
Topic: 36 Other Topic
Clinical features of aseptic meningitis
D. Fujii, H. Mori, K. Shindo. Department of Neurology, Kurashiki Central
Hospital, Kurashiki-shi, Japan
Background: Aseptic meningitis is a self-limited common disease.
But there are few reports about the clinical course, the laboratory
Abstracts / Journal of the Neurological Sciences 333 (2013) e679e727 e692