Name_________________________ ID___________________

Lab Day/Group Number: _______________________________

1

Labs 8 & 9 Genetic Profiling: Lab report

Research question:



Hypothesis:





Results:
1. Place your photo below.
2. Number each lane from left to right.
3. Indicate the sizes of each marker.
4. Fill in the table on the right with what the sample is, what volume it is.

5. The gel was a ____% agarose gel run in ____XTBE at _____V for ______minutes.


Lane
#
Contents
Lane
1



Lane
2



Lane
3



Lane
4



Lane
5



Lane
6



Lane
7



Lane
8






6. Measure the distances migrated for each of the size markers and record these measurements
in the table below.
7. Draw a standard curve using the semi-log graph paper provided.
8. Record the distance each band has migrated in the other lanes and determine the sizes of each
band from the standard curve. Record your measurements in the table.
Name_________________________ ID___________________
Lab Day/Group Number: _______________________________
2

Table :_______________________________________________________________

Lane 1: Marker Lane 2: Lane 3: Lane 4:
Band
#
Distance migrated
( )
Size (bp) Band
#
Distance migrated
( )
Size (bp) Band
#
Distance migrated
( )
Size (bp) Band
#
Distance migrated
( )
Size (bp)














Lane 5: Lane 6: Lane 7: Lane 8:
Band
#
Distance migrated
( )
Size (bp) Band
#
Distance migrated
( )
Size (bp) Band
#
Distance migrated
( )
Size (bp) Band
#
Distance migrated
( )
Size (bp)














Name_________________________ ID___________________
Lab Day/Group Number: _______________________________
3



Y

a
x
i
s
:

_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_

X axis: ___________________________________________
Title: ___________________________________________
Name_________________________ ID___________________
Lab Day/Group Number: _______________________________
4


Description of results
Describe your results by considering the following:
1. The quality of the gel and how it ran
2. The number of bands seen in each lane compared with what was expected.
3. Is the person homozygous or heterozygous? Justify your answer based on your data.
4. The sizes of bands seen in each lane compared with what was expected.
5. How many repeats of the D1S80 VNTR are present in the persons DNA? Show your
calculation.















Name_________________________ ID___________________
Lab Day/Group Number: _______________________________
5

Conclusions:
Interpret your results by considering the following.
1. From your data, what did you learn about VNTRs?
2. From your data what did you learn about how to analyse VNTRs?
3. Did you get the expected results? If not, why not?
4. What were the limitations of the techniques?
5. What were the limitations of the analysis?
6. Did you answer your research question?
7. Do your results support your hypothesis?
8. How could the experiment be improved?
9. What could be done next to study this VNTR?