1. Which of the following pairs are anomers?

a) Ribose and Ribulose

b) Glucose and mannose
c) Pyruvate lactate
d) -D-glucose and -D-glucose
2. Hybridoma technology is used in the production of
a) mRNA
b) Monoclonal serum
c) Monoclonal antibodies
d) Polyclonal antibodies
3. For mitochondrial structure and function mt-DNA specifies
a) 10%
b) 20%
c) 30%
d) 40%
4. Which of the following reagent is useful for visualizing DNA?
a) Uracil
b) DNA polymerase
c) Diphenylamine
d) Ethidium bromide
5. Homologous pairs line up along equatorial plane during
a) Anaphase II
b) Metaphase I
c) Telophase
d) Interphase
6. Between mitotic divisions, the cell is in
a) G
0

b) G
1

c) G
2

d) S phase
7. Most plant cells are surrounded by a rigid cell wall made primarily of
a) Monosaccharides
b) Pectin
c) Chitins
d) Polysaccharides
8. The organelle that functions in the breakdown of cells and tissues is the
a) Episomes
b) Mitochondrion
c) Centrosomes
d) Lysosomes
9. Endoplasmic reticulam
a) Is found only in animals
b) is a system of membrane bound channels
c) is called rough if mitochondria is attached to it
d) is a site of ATP production
10. 9+2 describes the basic structure of which of the following one
a) chromosome
b) Basal body
c) Chloroplast
d) Flagellum
11. The function of nucleus includes
a) Cellular respiration
b) Synthesis of proteins
c) Housing the hereditary information
d) Synthesis of carbohydrates
12. Bacculoviruses
a) RNA viruses
b) DNA viruses
c) Both a and b
d) None of the above
13. Secondary metabolites like antibiotics are not essential for
a) Log phase growth
b) Exponential growth
c) Both a and b
d) None of these
14. The restriction enzymes useful to the molecular biologist belongs to
a) Type I
b) Type II
c) Type III
d) Type IV
15. Which of the following vector is suitable for DNA sequencing
a) EMBL
b)PBR 322
c) M 13
d) Lambda
16. During which process, the free end of the chromosome moves from Hfr
donor in to the F cell across an intracellular bridge called piles
a) Transformation
b) Transduction
c) Conjugation
d) All of these
17. Francis Jacob and Monod Proposed
a) Lock and Key Hypothesis
b)Gene for Protein Synthesis
c) Operon Synthesis
d) Gene for protein synthesis
18. State which of the following is not a desired characteristic of a vector?
a) Unique restriction site
b) Large Size
c) Gene that confer antibiotic resistance
d) Autonomous replication
19. Which of the following is a carrier hosts for new genes?
a) EcoRI
b) Bacteriophages
c) Nucleus
d) Transplanted Organs
20. Which of the following chemical is used for preparation of competent cell?
a) HCl
b) NaCl
c) CaCl
2

d) Glycine
Answers
1.d) -D-glucose and -D-glucose 2.c) Monoclonal antibodies
3.a) 10% 4. d) Ethidium bromide 5.b) Metaphase I
6.b) G
1
7.d) Poly saccharides 8.d) Lysosomes
9.b) is a system of membrane bound channels 10.d) Flagellum
11.c) Housing the hereditary information 12. b)DNA viruses
13.c) Both a and b 14.b) Type II 15.c) M 13 16.c) Conjugation
17.c) Operon Synthesis 18.b) Large Size 19.b) Bacteriophages 20.c) CaCl
2

Must Read: Previous Questions on Agricultural Biotechnology (Questions: 21-40, 41-60, 61-80)




21. Endochitinase can be used as an
a) Antibacterial
b) Antiviral
c) Antifungal
d) Antinematodal
22. The direct DNA uptake by the protoplast is stimulated by the chemical
a) Cellulose
b) Sucrose
c) PEG
d) Mannose
23. Which of the following is an herbicide resistant gene?
a) Bar
b) Hpt
c) Chitinase
d) oxidase
24. Restriction enzymes that cuts straight across the DNA produces
a) Nick
b) Sticky ends
c) Blunt ends
d) None of these
25. Lambda has short single stranded 5 projection of
a) 8 nucleotide length
b) 10 nucleotide length
c) 12 nucleotide length
d) 14 nucleotide length
26. In Agrobacterium mediated plant transformation which one of the following
is used to check the Agrobacterium growth
a) PPT
b) Kanamycin
c) Timentin
d) Pencillin
27. The T-DNA region of all the Ti and Ri plasmids are flanked by direct repeat
sequence
a) 15bp
b) 20 bp
c) 25 bp
d) 35 bp

28. During Agrobacterium infections the plant cell begins to synthesise an
Arginine derivative called as
a) Hygromycin
b) Opines
c) Acetobenzylpurine
d) Acetosyringone
29. During Agrobacterium transformation the T-DNA is coated by protein,
which is the product of gene
a) Vir B
b) Vir C
c) Vir D
d) Vir E
30. Most commonly used strategy to develop transgenic plant against any viral
disease is to engineer a
a) Anti sense RNA for viral genome
b) Coat or capsid protein gene
c) Direct DNA transfer to viral gene
d) Liposome Mediated transfer of viral RNA
31. The vir region is organised into six operons viz., vir A, vir B, vir C, vir D, vir
E, vir G out of these which four operons are required for virulence
a) A,B,C,D
b) B,C,D,E
c) C,D,E,G
d) A,B,D,G
32. Replication of DNA by rolling circle mechanism give rise to a
a) Blunt end DNA
b) Theta structure
c) Concatamer
d) Single strand DNA with nick
33. . The gene transferred from E.coli to tobacco to increase the level of
manitol is
a) Manitol dehydrogenase
b) Manitol carboxylase
c) Manitol oxygenase
d) Manitol hydrolase

34. Relaxed plasmid
a) Have only single copy per cell
b) Cannot be purified
c) Does not exist in bacteria
d) Have multiple copies per cell
35. Which of the following is a green tissue specific promoter?
a) Pea vicilin
b) Arabidopsis small subunit of RUBISCO
c) 35s CaMV
d) Bean phytahemaglutinin
36. In vitro packaging is required for which one of the following vector
a) BAC
b) YAC
c) PAC
d) None of these
37. Cosmids have the Cos site of
a) Lambda
b) Plasmid
c) ML3
d) YAC
38. Thiobacillus thioxidans grows on
a) Irron
b)Sulphur
c) Aluminium
d) Pyrites
39. An example of commercially important primary metabolites is
a) Toxin
b) Alkaloid
c) Amine
d) Alcohol
40. Secondary metabolites like antibiotics are not essential for
a) Log phase growth
b) Exponential growth
c) Both a and b
d) none of these
Learn more : Notes / MCQs /Videos/ ICAR ARS NET Previous Question Papers
Answers:
21. c) Antifungal
22. c) PEG
23. a) Bar
24. c) Blunt ends
25. c) 12 nucleotide length
26. c) Timentin
27. c) 25 bp
28. b) Opines
29. d) Vir E
30. b) Coat or capsid protein gene
31. d) A,B,D,G
32. c) Concatamer
33. a) Manitol dehydrogenase
34. d) Have multiple copies per cell
35. b) Arabidopsis small subunit of RUBISCO
36. c) PAC
37. a) Lambda
38. d) Pyrites
39. d) Alcohol
40. c) Both a and b



ICAR ARS NET Previous Questions on Agricultural Biotechnology
41 Pure line theory was given by
a) Bateson
b) Johannson
c) Mendel
d) Morgan
42. Which of the following enzyme is used for making cDNA
a) DNA ligase
b) Taq polymerase
c) Restriction enzyme
d) Reverse transcriptase
43. The chemical used as an inducer of the vir operons is
a) Kanamycin
b) Hygromycin
c) Pencillin
d) Acetosyringone
44.9+2 describes the basic structure of which of the following one
a)Chloroplast
b) Flagellum
c) Mitochondria
d) Lysosome
45. The organelle that functions in the breakdown of cells and tissues is the
a) Centrosomes
b) Centriole
c) Lysosome
d) Liposome
46. An example for naturally occurring Auxin
a) IAA
b) ABA
c) BAP
d) 2, 4-D
47. A general method to confirm expression of transgene at translation level is
a) western blotting
b) northern blotting
c) eastern blotting
d) southern blotting
48. The browning of culture medium induced by some explants can be
prevented by the addition of
a) GA
b) 2-4-D
c) IBA
d) PVP (polyvinyl pyrollidone)
49. Which of the following is not used as a cryoprotectant
a) glycerol
b) DMSO
c) polyclar
d) PEG
50. Anther culture provide a method for the production of
a) polyploidy
b) heterozygous line
c) apomictic line
d) homozygous line
51. The first stable product of photosynthesis
a) glucose
b) DHAP
c) RUBP
d) 3PGA
52. Oxidation of water during photosynthesis involves
a) PSI
b) PS II
c) both
d) none of the above
53. Which one of the following chemical causes frame shift mutation
a) 2-amino purine
b) hydroxylamine
c) 2 bromo uracil
d) acridine orange
54. The restriction enzyme useful to the molecular biologist belongs to
a) Type I
b) Type II
c) Type III
d) both a and b.
55. A good molecular marker must be
a) polymorphic
b) heritable
c) both a and b
d) none
56. Shortgun approach is used in
a) gene mapping
b) DNA sequencing
c) gene transformation
d) genomic library
57. The length of Okazaki fragments in E.coli are about
a) 100-200 nucleotides
b) 500-1000 nucleotides
c) 1000-2000 nucleotides
d)10000-100000 nucleotides
58. The sigma factor of RNA polymerase in E.coli involved
a) RNA synthesis
b) promoter binding
c) transcription initiation
d) binding with template DNA
59. The Casparian stripes is present in
a) epidermis
b)endodermis
c) cortex
d) root hair
60. Flavr Savr is a variety of
a)potato
b) tomato
c)pomato
d)avocado
Learn more: Notes/ MCQs/ Videos/ Previous Question Papers/ ARS-NET-Books to
Refer

Answers: 41-b, 42-d, 43-d, 44-b, 45-c, 46-a,47-a, 48-d, 49-d,50-d, 51-d, 52-b, 53-d,
54-b, 55-c, 56-d, 57-c, 58-b, 59-b, 60-b.





Previous question papers

61. During Agrobacterium infections the plant cell begins to synthesize an
Arginine derivative called as
Opines
62. Enzyme used for making cDNA
Reverse transcriptase
63. Reagent used for visualizing DNA?
Ethidium Bromide (EtBr)
64. Enzyme used in PCR (Polymerase Chain Reaction)
Taq polymerase (Taq: Thermus aquaticus)
65. PCR invented by
Kary Mullis
66. In PCR, the double stranded DNA is generally denatured by
Temperature
67. DBM paper is used for
Northern Blotting
68. Number of base pair units in a single turn of DNA is
10
69. Father of Plant Tissue culture
Haberlandt
70. A good molecular marker must be
Polymorphic, Heritable
71. Acid fast staining used for which bacteria?
Mycobacterium
72. Inhibitory action of Penicillin is
Cell wall synthesis
73. DNA staining is done by
Feulgen staining
74. The most widely used chemical for protoplast fusion, as fusogens, is
PEG(Poly Ethylene Glycol)
75. In order to obtained virus free plants through tissue culture the best method
is
Meristem culture
76. Endochitinase can be used as an
Antifungal
77. Flavr Savr is a variety of
tomato
78. The chemical used as an inducer of the vir operons is
Acetosyringone
79.The restriction enzyme useful to the molecular biologist belongs to
Type II
80. Which amino acids can stabilize protein structure by forming covalent cross
links between polypeptide chains ?
Sulphur