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• Detectors:
• UV- a phototube (photocell) is used , as well as in visible, can be very specific for
different regions of the spectrum. Also, photomultiplier tube – more sensitive than
phototube. Third type id CCD charge-coupled device
• IR- poses property of heat, and heat detectors that transduce heat into an electrical
signal can be used. Thermocouples and bolometers are used. For rapid
measurements with FTIR instruments, and for high sensitivity measurements,
photon detectors are used.
•
• IR:
• Absorbing (vibrating) groups in the infrared region absorbs withing a certain
wavelength region, and exact wavelength will be influenced by the neighbouring
groups. Absorbtion peaks are so much sharper than UV, each molecule has a
fingerprint region (specific for each molecule).
• Most important use of IR is identification and structure analysis, it could also be
used for quantitative analysis of complex mixtures of similar compounds (with
intensities proportional to the concentration of absorbing species).
• Can have rotational/vibrational –(see notes above) transitions (far-infrared),
• For Vibrational modes:
• Quantitative analysis:
• Beer-Lambert Law generally applies:
• Determine peak baseline
• Measure height
• Convert to absorbance units
• A=-log T=-log (%T/100)
• Quantitation relies on pathlength
• -use liquid cell
• -use internal standard (resolve IR peaks)
• -plot Astd/AIS vs. Cstd.
• -add same amount of IS to sample
• -record Asample/AIS
• Two forms of IR :
• 1.) Dispersive
• -uses a grating monochromator
• -takes time to scan wavenumber range
• -need to calibrate wavenumber position using a standard
•
• 2.)Fourier Transform Infrared Spectroscopy (FTIR)
• -uses a interferometer
• -advantages of FTIR – greater throughput, increased signal –to-noise ration,
simultaneous measurement of all wavelengths.