8ewlrlng Cell Surfaces for 1herapeuuc Appllcauons

ln 1lssue Lnglneerlng, ulsease and Cancer

1
List of drugs
As of 2008, 11 drugs with liposomal delivery systems have been approved and 6 additional
liposomal drugs were in clinical trials.[10]
List of clinically approved liposomal drugs
Name Trade name Company Indication
Liposomal amphotericin B Abelcet Enzon Fungal infections
Liposomal amphotericin B Ambisome Gilead Sciences Fungal and protozoal infections
Liposomal cytarabine Depocyt Pacira (formerly SkyePharma) Malignant lymphomatous meningitis
Liposomal daunorubicin DaunoXome Gilead Sciences HIV-related Kaposi!s sarcoma
Liposomal doxorubicin Myocet Zeneus
Combination therapy with cyclophosphamide in metastatic
breast cancer
Liposomal IRIV vaccine Epaxal Berna Biotech Hepatitis A
Liposomal IRIV vaccine Inflexal V Berna Biotech Influenza
Liposomal morphine DepoDur SkyePharma, Endo Postsurgical analgesia
Liposomal verteporfin Visudyne QLT, Novartis
Age-related macular degeneration, pathologic myopia, ocular
histoplasmosis
Liposome-PEG doxorubicin Doxil/Caelyx
Ortho Biotech, Schering-
Plough
HIV-related Kaposi!s sarcoma, metastatic breast cancer,
metastatic ovarian cancer
Micellular estradiol Estrasorb Novavax Menopausal therapy

LmpLy?
nanoparucle wlLh noLhlng speclal
lnslde?
We care abouL llplds
Llposome
Challenge:

uellver new funcuonallLy Lo Cells for a range of dlagnosuc
1herapeuuc and 1lssue Lnglneerlng Appllcauons. (SLem Cell lasuclLy).
-The ability to spatially and temporally direct 3D cell assembly comprised of multiple
cell lines is crucial for next generation therapeutic applications in immunity and
regenerative medicine.

-In vivo Imaging, tracking and targeted drug delivery to specific cells, cell tissues and
drug resistant bacteria is critical for many diagnostic biotechnologies, vaccine
development and drug screening platforms.

-These research fields and markets are diverse but converge at the ability to rewire
(engineer) cell surfaces, which can subsequently be tailored for specific
applications.
lnLroducuon
Cell-Surface
Lnglneerlng
8egenerauve
Medlclne
8lo-lmaglng
and
Senslng
Cell Largeung and
Cell 1herapy
1lssue
Lnglneerlng
ConLrolllng "#$$ %&'()*# lnLeracuons: key Lo +,,$-*)./01 ln Cell 8lology/Medlclne
New Model System
O
O
NH
2
O
O
H
2
N
Liposome Adhesion Liposome Fusion
Oxime Bond
Formation
O
O
NH
2
+
Liposome-Liposome Fusion via Oxime Bond Formation
Neutral POPC
(95 %)
Liposome Functionalization with Alkyl Ketones and Oxyamines (5 %)
1nm 30nm 1m-100m 100m-mm mm-cm cm-dm meLer
Cell-
Surface
Lnglneerlng
1lssue
Assembly
Llposome
luslon
Llposome
lormauon
Llpld
SynLhesls
1lssue
CrowLh
1lssue
Moldlng
1lssue
1ransplanL
llpld llposome Lnglneered Cell Spherolds 1lssue Crgan
unlque 2'/*#11 for Ceneraung 1lssue 8ased on 1allored Cell Surfaces
vla Llposome luslon and 8lo-orLhogonal Llplds
llpld Llposome Lnglneered Cell Spherolds
llposome Lnglneered Cell Crgan
new 8loreacLors and 3u 1lssue lormauon
8loreacLor
Complex 1lssue
3u 1lssue rlnLer
1ransplanL Llposomes Cells Lnglneered Cell
8loreacLor 1echnology
3u 1lssue lormauon