Yiding Yan

617-759-9754, yidingyan@rcn.com

SUMMARY
Senior Biologist with in-vitro and in-vivo experiences. Expertise includes cell-based and biochemical
assays development, small-molecule and siRNA screening, data analysis and interpretation, primary
cell work, molecular biology techniques, and murine/rat transgenic model development. Dedicated
individual with ability to learn quickly. Able to work well both independently and in a team environment.
TECHNICAL SKILLS
Cell Biology
• Expert in developing and performing in vitro cell-based assays, including cell proliferation,
apoptosis, GFP reporter assays, ELISA assay, radiometric enzyme kinetic assay, embryonic
stem cell differentiation assay, alkaline phosphatase activity assay, and bDNA assay.
• Expert in vitro cell-based screens, including siRNA, small molecule compound and cDNA
library.
• Expert in vitro cell culture techniques; primary osteoblast cell isolation, retroviral production,
infection, and stable cell line generation.
• Familiar in immunstaining in both mouse tissue and living cell, and protein
immunoprecipitation.
• Experienced in HRP conjugated/purification, and in using guava flow cytometry.
Molecular Biology
• Extensive experience with modern techniques in molecular biology including: genetic gene
construction, conditional knock in /out mouse construction, transgenic construction, gene
switch transgenic system, gene recombination by using yeast system, artificial bacterial DNA
library screening and purification, targeting mouse ES cell selection, mouse tail genotyping.
• Expert in DNA, RNA isolation, PCR, Taqman-RCR, site –directed mutagenesis,
and subcloning DNA/fragment into expression vectors, and primer designing.
• Familiar with bioinformatics database: BLAST
Mouse Genetics
• Experienced in proficiency of transgenic mouse/ rat organ isolation, necropsy.
• Experienced in proficiency of mouse subcutaneous with retroviral mutagenesis tumor model.
Compute and Automation
• Extensive experience with Prism-graph, MS word, Excel, PowerPoint and data analysis.
• Experience in Platemate plus; Biomek FX; Fluorometric Image Plate Reader.

PROFESSIONAL EXPERINCES:
Pfizer Cambridge, MA 2008-Present
Scientist III, Cell biology (8/08-present)
Contract Research Scientist (4/08 – 8/08)
• Established siRNA cell-based assay; evaluated and optimized transfection conditions in
multiple tumor cell-lines to assess potency of therapeutic lead molecules.
• Validated bDNA assays to measure mRNA knock down and IC50 values in cell based assay.
• Developed anti-proliferation and caspase based apoptosis assays and validated molecular
targets for human hepatocellular carcinomas.

Shire Pharmaceuticals Lexington, MA 2007-2008

Senior Scientific Specialist II, Cell Biology)
• Established cell-based ELISA assays, evaluated therapeutic fusion protein binding affinity and
uptake Kd, and characterized fusion protein binding conditions by Weston blot.
• Developed apoptosis cell-based assays and cell proliferation assays for evaluation of TNF-
alpha fusion protein uptake utilizing a cell flow cytometry assay.

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 Sanofi-Aventis/AVEO Cambridge, MA 2006
Contract Scientist, Functional Knockdown / Tumor modeling
Yiding Yan

• Developed both ATPlite cell proliferation assays and cAMP assays for the validation of lead
cancer inhibitor compounds.
• Participated in developed animal tumor model for the purpose of identifying genes and
pathways involved in tumor maintenance by conducted retroviral mutagenesis and IP injected.

Millennium Pharmaceuticals Cambridge, MA 2005

Research Investigator, Inflammation Cell Signaling Biochemistry
• Supported enzyme target projects within the Inflammation department, developed enzymatic
assays, and characterized small molecule inhibitors, including IC50 and Ki determinations.
• Developed biochemical endpoint sandwich ELISA assays for the screening of compounds on
cell signaling pathways, and analyzed the pathway data.
• Dissected and mapped protein kinase signal transduction pathways by using mutagenesis of the
kinase in response to stress using Western blot technique.

Wyeth Andover, MA 2001- 2005

Research Scientist, Molecular Genetics
• Generated a gene construct for use in developing a high bone mass transgenic mouse model.
Established primary cell-based proliferation assays and performed genotyping of transgenic mice to
characterize the animal model.
• Cloned neutrophil elastase gene for use in developing a transgenic mouse model for respiratory
disease. Validated transgenic mouse model through cell-based and genotyping assays.
• Generated human MMP12 gene knockout/in construct using a yeast recombination system to be
used for the generation of emphysema disease mice models.

Curis Inc. Cambridge, MA 1997- 2001

Research Associate, Developmental Biology
• Established a retroviral cell system for identifying factors/genes that drive the differentiation of
embryonic stem cells to the TH+ neuron stage, and pancreatic beta cells.
• Developed a GFP cell-based assay for targeting novel genes in SHH signaling pathway using
cDNA libraries, and provided transient transfections to support company-wide research projects.

Cardiology Division of Beth Israel Hospital Boston, MA 1994 – 1997

Boston University Medical School 1992 - 1994
Research Associate,
• Studied a cell cycle gene family involved in cell quiescence and senescence. Analyzed the
synthesis and degradation of cyclin-like mRNA during cellular life-span.
• Identified and expressed photosynthetic genes. Evaluated enzymatic activity assays to measure
polymerase activity from chloroplasts.

Education:
Bachelor of Medicine Beijing Capital Medical University, Beijing, China
(Degree was evaluated by US that equivalent to US Masters Degree in a scientific field)
Continue Education:
Cell Biology course Harvard Extension
Biochemistry course Boston University

Publications (selected):
• Troxler, R.F., Yan, Yiding, Jiang J, and Liu B. Nucleotide Sequence and expression of the genes
for the alpha and beta subunits of phycocyanin in Cyanidium caldarium. Plant physiology 107(3): 985-
994 (Mar 1995).

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• Jiang J, Liu B., Yan, Yiding, and Troxler, R. F. Differential binding of protein factors to upstream
regions of PsbA in Cyanidium caldarium plastid in vitro transcription system. Annual meeting of Plant
Physiology in 1994

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