Summer 2014 - Biology 021 Lab Schedule- Enriquez

All consumable materials are listed per lab section !0 students"#

$une 1% : &he Scienti'ic (ethod E)ercise 1"
1. 6 drafting compasses with pointy ends
2. 6 plastic rulers (6-inch)
3. 20 Cotton alls in ea!er " #o $e %isposed after &se'
(. 6 #uning for!s
). 6 *rotractors
6. 6 #hermometers
$une 1* : (easurement E)ercise 2"
+ection 2.1: ,etric +ystem
1. 6 rulers with oth inch and centimeter units on opposite edges (12- preferred. 6-
o!)
2. 6 2)0m/-ea!ers
3. 6 2)0m/- 0rlenmeyer flas!s
(. 6 10m/-graduated cylinders
). 6 2)m/- graduated cylinders
6. 6 100m/-graduated cylinders
1. 6 1-2t 3ars or ottles with fill line
4. 6 plastic dropping pipettes (not graduated)
5. 6 1-pound pac!ages of anything (pasta. coffee. etc.)
10. 6 coffee mugs. cups or glasses
11. 6 1-gallon mil! ottles
12. 6 tape measures
13. 6 thermometers
1(. 6 hot plates
1). oiling chips (enough for 6 groups)
16. 6 electronic scales (as precise as possile6 0.01 g at least)
11. one gallon d728
14. athroom weight scale
15. ice uc!et with ice
$une 1+ : ,omeostasis E)ercise 4"
+ection (.2: 9egulation of p7
1. 6 plastic trays
2. 30 100m/-ea!ers
3. 6 glass stirring rods
(. mas!ing tape
). 6 sets of p7 test papers with color scale
6. ) )m/-pipettes laeled :;inegar-. :water-. : 8.<.-. :detergent-. :mil!-
1. 6 green pipetters
4. )00m/ container of non-phosphate detergent solution (1=(teaspoon=)00m/)
5. carton of mil! (200m/=section)
10. carton orange 3uice (200m/=section)
11. )00m/ ottle of white ;inegar
12. )00m/ d728
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13. 6 )m/- pipettes
$une 1-. (acromolecules E)ercise /" 0 1oggles needed
+ection ).1. ).2: Carohydrates ($enedict>s and /ugol>s tests)
1. )0 small test tues
2. 6 test tue rac!s
3. ?t least 3 test tue rushes
(. 6 wa@ pencils
). mas!ing tape
6. 6 test tue clamps=holders
1. 6 hot plates
4. 6 heat-resistant glo;es
5. 6 2)0- or (00-m/ ea!er
10. oiling chips
11. stoc! solutions: each stoc! solution needs to ha;e its own laeled
one-piece plastic dropping pipette.
?. 10m/ 6A stoc! solutions 0?C7: glucose. fructose. starch
$. 20m/ 0?C7: cream. onion 3uice. potato 3uice
C. 10m/ 0?C7: lemon 3uice. clear diet soda and non-diet soda
%. 100m/ d728
0. 3 1)m/ un!nowns
12. 6 20m/ /ugol>s solution in dropper ottles
13. 6 20m/ $enedict>s reagent in dropper ottles
+ection ).3: /ipid #est
1. stoc! solutions: each needs to ha;e its own laeled
one-piece plastic dropping pipette.
?. 10m/ ;egetale oil
$. 1)m/ hamurger 3uice
2. 6 20m/ +udan BC in dropper ottles
+ection ).(: *rotein test
1. stoc! solutions: each needs to ha;e its own laeled
one-piece plastic dropping pipette.
?. 2m/ egg alumin
2. 6 20m/ $iuret reagents in dropper ottles
Dote for instructors:
o 0ach group is assigned with either $enedict>s (#ale )-1). or /ugol>s (#ale )-2).
or +udan BC ()-3). or $iuret ()-() test.
?ssign $enedict>s and /ugo>s tests to two groups since there are
6 groups in la. +hare data.
o Bn addition. each group needs to test one un!nown solution (#ale)-)) for
presence of simple sugars. comple@ carohydrates. lipids and proteins.
$une 2!. (icroscopy E)ercise !"
+ection 3.1: Compound /ight ,icroscope
1. 24-30 compound light microscopes (one=student)
2. lens paper
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3. s2ueeEe-wash ottle containing 10A alcohol
(. 10 slides of each of the following:
letter :e-
smeared lood
Oscillatoria
large neurons or other cell types
:3-colored threads-
+ection 3.2. 3.3: 7ow to ,a!e a Fet ,ount. and ,icroscopic 8ser;ations
1. 6 dropper ottles containing d728
2. o@ of glass microscope slides (for 0@. 3 and 6)
3. o@ of co;er slips (for 0@. 3 and 6)
(. pond water or some other mi@ed cultures
). reference oo!s to identify the microorganisms in pond water
6. 6 dropper ottles of *rotoslo
+ection 3.(: %issecting microscope
1. 24-30 dissecting microscopes
2. 24-30 specimens for ;iewing (small flowers. insects. lea;es. etc.)
Structure and 2unction o' Li3ing 4ells E)ercise %"
+ection 6.1:*ro!aryotic cells
1. ;ial of Cyanoacteria
2. two pasteur pipettes laeled :Cyanoacteria-
3. 3 %0,8D+#9?#B8D-8D/G microiology slides of acteria 3 shapes (coccus.
acillus. spirillum)
+ection 6.2: 0u!aryotic cells
1. 32 toothpic!s
2. 6 forceps
3. 6 dropper ottles containing d728
(. 6 dropper ottles containing methylene lue
). 0lodea
6. one onion ul
1. animal cell model and !ey
4. plant cell model and !ey
5. pro!aryotic cell model (or a poster if we don>t ha;e any)
10. 2 2)0-m/ ea!ers containing 10A leach for toothpic! disposal
11. 2 )00-m/ ea!ers containing 10A leach for chee! cells wet mounts
disposal
$une 24. 5i''usion and 6smosis. E)ercise *"
+ection 1.1: 0ffect of +olute Concentration on 8smosis
1. 2( 1)-cm lengths of dialysis tuing. soa!ing in d728
2. (4 dialysis tuing clamps
3. 14 10-ml pipettes
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(. 6 pipetters
). 2( (00-ml ea!ers
6. dishpan half-filled with d728
1. paper towels
4. 6 alances
5. 100m/ 1)A sucrose solution
10. 1(00m/ 30A sucrose solutions
11. 2 10-m/ pipettes laeled :1)A sucrose- and pipetters
12. 2 10-m/ pipettes laeled :30A sucrose- and pipetters
13. 2 10-m/ pipettes laeled :d728- and pipetters
+ection 1.(: *lasmolysis in *lant Cells
1. 2 forceps
2. o@ of microscope slides
3. o@ of co;er slips
(. 0lodea in water
). 2 dropper ottles containing d728
6. 2 dropper ottles containing 20A DaCl
$une 2/. Enzymes E)ercise +" - 1oggles needed
+ection 4.1. 4.2?. 4.2$. 4.2C: ,easuring Catalase ?cti;ity
1. ea!er of li;er e@tract (20m/)
2. 6 2)m/- graduated cylinders
3. 6 10m/- graduated cylinders
3. 6 rectangular pans
(. 6 forceps
). 6 stoppered ottle with tues inserted to collect gas
6. filter paper
1. hole punch
4. source of d728 near sin!s
5. ice uc!et
10. water ath at 31 degree C
11. 2 thermometers
12. 20m/ each of phosphate uffers p7(. 1. and 10
13. 3 )-m/ pipettes laeled :p7(-. :p71-. :p710-. and 3 pipetters
1(. 2 ottles or stoppered flas!s. each containing 100m/ fresh 7282
1). 2 10-m/ pipettes laeled :7282-. and pipetters
Dote to instructors: %i;ide the wor! etween 6 groups. and share data
0ach group is assigned with either a specific temperature or p7 or enEyme
concentration
$une 2%. 7hotosynthesis E)ercise -"
+ection 5.1: &pta!e of Caron %io@ide during *hotosynthesis
1. 0lodea in water
2. 6 lamps
3. 12 )0-m/ graduated cylinders
(. 6 2)0-m/ ea!ers
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). 10 straws
6. 6 rulers
1. 3 raEor lades or small scissors
4. 100m/ diluted phenol red solution
5. colored cellophane (plastic that turns white light different colors. e.g. red.
green. lue)
+ection 5.2: 9elease of 8@ygen in *hotosynthesis
1. 1000m/ 0.2A sodium icaronate solution in flas! or ottle
2. 6 dropper ottles containing 20m/ diluted li2uid soap
3. 12 100-m/ ea!ers
(. 12 plastic 10-cc syringes
). leaf material (hairy leaf will not wor!) " each group will need 20 leaf dis!s
6. hole punch
1. sharpie or other fine-tipped mar!er
4. light source
5. source of d728 near sin!s
$une !0. 58A Structure E)ercise 14#2"
1. %D?-puEEles with handouts for %D? replication. transcription and translation
2. 2 %D?-models with !eys
Dote to Bnstructors: ?ssign section 5.1 to 3 groups. and section 5.2 to remaining 3 groups.
+hare data.
E)ercise 14#1A. 9solation o' 58A
1. 6 small funnels with cheesecloth s2uares
2. 2( small Hiploc ags
3. 2( test tues
(. 6 stirring rods
). 6 test tue rac!s
6. ice uc!et
1. 2( froEen strawerries. thawed
4. 100m/ ice cold 5)A ethanol di;ided into two s2ueeEe-ottles
5. 100m/ detergent (10A woolite preferred) and 1A DaCl solution in flas! fitted with a
)-m/ pipette and pipetter
10. 8ne 10-m/ pipette (laeled alcohol) and pipetter
$uly 01. (itosis and 4yto:inesis E)ercise 11"
+ection 11.2: #he Cell Cycle in *lant Cells: 8nion 9oots
1. 24-30 compound microscopes
2. 30 slides of onion. ?llium. and root tip mitosis
3. chromosome model
+ection 11.3: #he Cell Cycle in ?nimal Cells
1. 30 slides of whitefish lastula
+ection 11.(: +imulating ,itosis
1. 6 sets of ((-eads 0?C7 of yellow and pin! or red
2. (4 magnetic centromeres
3. strings
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(. 7uard chart of mitosis
). *oster of mitosis
$uly 02. (eiosis E)ercise 12"
+ection 12.1: %emonstration of ,eiosis using pop eads
1. 7uard chart of meiosis
2. *oster of meiosis
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$uly 0!. ,uman ,eredity E)ercise 1!"
1. *#C taste papers. enough for 30 students
$uly 0*. 7edigree Analysis
?ppendi@ 2: Jenetic prolems
Do material re2uired
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$uly 0-. E3olution Lab E)ercise 1*"; pages 4*/-4** and 4+0
+ection 11.1: Datural +election
1. 12 +poons
2. 12 forceps
3. 1 l dry na;y eans
(. 1 l dry lac! eans
). 1 l dry lentil eans
6. 1 l dry lima eans
1. 1 l dry garanEo eans (chic!peas)
4. 2 ls lac! a2uarium gra;el
5. 2 ls white a2uarium gra;el
10. 12 medium siEed dissection trays
$uly 0-. E3olution Lab E)ercise 1*"; pages 4*/-4** and 4+0
+ection 11.2: Jeologic #ime
11. ,etric tape
+ection 11.3: #he Kossil 9ecord and 7uman 0;olution
12. +!ulls of modern human. Australopithecus,
Homo erectus. and Deanderthal
13. 7uman s!eleton (articulated)
1(. Korelim homologous set (2)
1). 7ind lim homologous set (2)
16. Certerates s!ull set
11. Krog s!eleton
14. $ird s!eleton
15. *lastic mount of /iEard s!eleton
20. *lastic mount of at s!eleton
21. *lastic mount of whole at
22. *lastic mount of pig emryo
23. +na!e s!eleton
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2(. Cat s!eleton
2). #urtle s!eleton
$uly 10. Bacteria and 7rotista E)ercise 1- "
+ection 15.1: ?re acteria present in the laL
1. ) Dutrient agar plates=section
2. sterile cotton swas
3. 6 wa@ pencils
(. ottle containing tap water
). ottle containing 10A alcohol
6. posters of 3-shapes acteria (acilli. cocci. spirilla if we ha;e any)
Bacteria and 7rotista E)ercise 1- and 20" continued.
1. poster of :*rotista- or :*rotoEoa-
2. poster of :?lgae- (if we ha;e any)
3. ri!er mounts of rown algae. red algae. green algae
(. do we ha;e a poster of :slime mold-L
). %0,8. 8D/G $acterial types slide
6. %0,8. 8D/G culture plate of any acteria from micro la
1. petri plate containing Physarum. plasmodial stage
4. 3 dissecting needles
5. 6 slides of each of the following:
%esmids
Oscillatoria
Oedogonium
Volvox (if we ha;e any)
Paramecium
Euglena
Amoeba proteus
%iatoms
Spirogyra
Trypanosoma gambiens
Plasmodium vivax
Saprolegnia (water mold)
10. could we ha;e li;e:
Paramecium
Spirogyra
Volvox
Chlamydomonas
Oscillatoria
11. o@ of slides
12. o@ of co;er slips
13. Paramecium and Amoeba models with !eys
$uly 14. 2ungi E)ercise 2/"
+ection 2).3: $read mold and food preser;ati;es
1. 6 droppers
2. 6 slices of read with :preser;ati;es-
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3. 6 slices of read with :no preser;ati;es-
(. o@ of small Eip loc! ags (at least 12)
). 3 culture tues of read mold (!hi"opus) spores suspension in roth
6. 3 20m/ sterile d728 in dropper ottles
2ungi E)ercise 2/" continued.
1. culture plates of the following:
read lac! mold (!hi"opus)
Penicillium
Aspergillus
2. 6 slides of each of the following:
!hi"opus Eygospore (se@ual spore)
!hi"opus sporangiospore (ase@ual spores)
Pe"i"a
3. preser;ed :
:cup fungi- (nest fungi) (dry)
:shelf fungi- (rac!et fungi) (dry)
puffall (dry)
lichens (dry)
(. plastic mount of lichen types
). model of edile mushroom
6. 6 fresh edile mushrooms
1. 6 dissecting needles
4. cutting oard
5. o@ of slides
10. o@ of co;er slips
$uly 1/. 7lant 5i3ersity 9
0@ercise 21: $ryophytes " /i;erworts and ,osses
1. *lastic mounts:
/i;erworth
$ryophytes
#hallophytes
,oss
:,oss collection-
#nium life history
2. +panish moss ri!er mount
3. *reser;ed:
sporophyte (in a glass 3ar in +112)
#archantia (in +112)
$ryophytes
#hallophytes
,oss
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). +lides:
#nium (moss) antheridia
#nium archegonia
#nium capsule (containing sporangium)
#nium sporophyte
moss protonema
6. #archantia life cycle model and !ey
0@ercise 22: +eedless Cascular *lants: Clu ,osses and Kerns
1. *lastic mounts:
$ycopodium (clu moss). and preser;ed
E%uisetum (horsetail) (in +112)
Kern Kronds (in +112)
Kern (in the white case in cainet ne@t to +112)
primiti;e ;ascular plants (in the same white case)
Selaginella (lycophyta or spi!e moss). and preser;ed
$ycopodium (clu moss) gametophyte. and preser;ed
Psilotum (whis! fern). and preser;ed
Kern gametophyteL +porophyteL
2. %o we ha;e Kern rhiEome (stem) slidesL
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$uly 1%. 7lant 5i3ersity 99
0@ercise 23: +eed *lants B: Jymnosperms
l. *lastic mounts:
Jin!go life history
H?,B? life history (male and female cones young and mature)
:C8,,8D JG,D8+*09,+-(general iological supply house. Chicago. Bll.)
*ine life history
Conifer twigs (in the white case)
2. *reser;ed:
male and female pine cones
Jymnosperm pine (staminate cone) (in little 3ar in +112)
Jymnosperm pine (o;ulate cone) (in little 3ar in +112)
3. +lides:
male pine cone
female pine cone
*ine emryo
0@ercise 2(: +eed *lants: ?ngiosperms
1. ,odels and !eys:
leaf
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apple lossom
/ily flower
2. *lastic mounts
cactus flower=fruit
monocot and dicot flowers
$ilium
/ima ean germination
$ilium entire flower
$ilium. anther. mature pollen
$ilium o;ary
Capsella emryo
3. $otany mount of seed dispersal display
$uly 1*. Animal 5i3ersity 9
0@ercise 26: +ponges and Cnidarians
1. *lastic mounts:
Obelia
Hydra
Aurelia
Coral mount laeled :$-
2. $iosmounts of:
Coral collection
Pori&era
Coelonterate
3. 9i!er mounts:
Kinger sponge
Kan coral
(. *reser;ed:
Scypha
%ry large sponge
%ry ;enus flower as!et (glass sponge)
). +lides:
Spongilla or Scypha (Jrantia) spicules
6. Could we ha;e li;e Hydra
0@ercise 21: Klatworms and 9otifers
1. *lastic mounts:
*lanaria
*latyhelminthes
2. 6 slides each:
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Taenia pisi&ormis or Taenia solium
Clonorchis sinensis
3. *reser;ed:
#apeworms
flu!es such as 'asciola hepatica
(. *lanaria model
). /i;e:
*lanaria
9otifers
6. o@ of slides
1. o@ of co;er slips
4. 2 pasteur pipettes laeled: :planaria-. and :rotifer-
0@ercise 24: +egmented Forms and ,ollus!s
1. *reser;ed mount of /umricus (earthworms). leech. lugworm. sandworm.
#ueworm. (9epresentati;e ?nnelids. fisher scientific)
2. $iosmounts:
?nnelids
,ollus!s (s2uid. oyster. scallop. limpet)
Kresh water mussel dissected
3. %issected s2uid mount
(. *reser;ed leeches
). *lastic mount of /eech
6. ,ussel model
$uly 21. Animal 5i3ersity 99
0@ercise 25: 9oundworms and <oint-/egged ?nimals
1. *reser;ed female and male Ascaris
2. *lastic mounts:
Ascaris
Crayfish (non-dissected)
,illipede-centipede
7orseshoe cra
$lac! widow spider
+corpion
,ain order of insects (Fard>s plastic mount)
3. 9i!er mounts
Centipede and millipede
#arantula
(. $iosmount of ?rthropoda (crayfish. shrimp. arnacles. cra)
0@ercise 30 : 0chinoderms and Bn;erterate Chordates
1. *lastic mounts:
+ea urchin
+tarfish
/ancelet
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2. %ry specimens of +ea stars (2)
3. $o@ trays:
0chinoderms
+ea stars
(. 9i!er mount containing sand dollar. +ea star sea urchin and sea cucumer
$rittle star. Keather star
). $iosmount of 0chinodermata
6. *reser;ed:
?mphio@us (lancelet)
+ea tunicates (sea s2uirts)
0@ercise 31: Certerates
1. *lastic mounts
/amprey (with laeled parts)
*8, 2130 /amprey
*erch
$ay turtle
+!ates
9ays
2. +har! 3aw
3. %ry specimens:
Krog. 9attle sna!e
$ird mounts
7aw!
$lac!ird
7ummingird
,ammals mount
9ait
+2uirrel
$at
(. *reser;ed specimens:
+alamander in 3ar
/iEard in 3ar
9attle sna!e in 3ar
/ung fish in 3ar
Krog de;elopment in cylindrical 3ar
+har!
$uly 22: 0cology B:
1. /egal or *oster paper (11@11 or larger) (at least 6)
2. ,ar!ers and=or coloring pencils
$uly 2!: 0cology BB: Dature wal!6 no materials needed
$uly 24: /a final
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