iiiiiiiiiiiiiiiiiiiiiiiii Founded 2007

International Journal of
Life Sciences
Mohsen Jo|or|on Dehkord|, Homed
So|eymon Dehkord|, Hom|d Ironpour
Moborokeh, FohomKhomes|pour ond
8ehzod Homed|
Internot|ono| Journo| o| L||e Sc|ences
ISSN No. 201-0525
DOI-
dx.do|.orgJ10.312J|j|s.v8|3.10433

Assessing tbe effect of tbe tbyme {1hyme
vulgurlx L.] essence on some biocbemical
factors in rat's blood serum
Tbis article is protected by copyrigbt, and all rigbts are beld exclusively by International )ournal of Life Sciences.
ACCESS
OFEN
I n t e r n o t | o n o | J o u r n o | o | L | | e S c | e n c e s 8 [ 3 } : 2 0 1 4 ; 1 5 - 1 8
An Independent, Open Access, Feer kev|ewed, Non-Fro||t Journo|
Webs|te: http:JJnepjo|.|n|oJ|ndex.phpJIJLSJ|ndex
Assessing the eIIect oI the thyme {Thgme oulgnris L.) essence on some biochemicul
Iuctors in rut's blood serum
Researcb Article
I,' 1 2
Moh:en lofor|on Dehhord| , Homed 5o|eymon Dehhord| , Hom|d |ronour Moboroheh ,
1 4
Fohomkhome:|our ondehzod Homed|
1
Ju1!DD! o] Tu!hoIoq_, uruI!_ o] `!11Du1_ ?n1r1D, 5huh1Io1n T1uDrh, J:IuD1r .zun 'D1:1:1!_, 5huh1Io1n, J1uD
2
uruI!_ o] `!11Du1_ ?n1r1D, 5huh1Io1n T1uDrh, J:IuD1r .zun 'D1:1:1!_, 5huh1Io1n, J1uD
_
\ouDq T:u1rh1: uDn JI1! CIuI, 5huh1Io1n T1uDrh, J:IuD1r .zun 'D1:1:1!_, 5huh1Io1n, J1uD

T:u1rh: CD!1 o] ?n1r1DuI TIuD!: & J!hDo-:!11Du1_ J:IuD1r .zun 'D1:1:1!_ o] 5huh1Io1n T1uDrh, 5huh1Io1n, J1uD
ArtIcIe InIormatIon ABSTRACT
Tbyme is a plant native to Iran tbat is widely found in soutbern part and mountainous center of country
and bas antibacterial and antifungal activity on digestive and cbronic diseases in traditional medicine.
However tbe medicinal species is called Tbyme vulgaris, all of tbe Tbyme are enricbed witb volatile
compounds including tbymol and carvacrol acting as strong antiseptic. In present study we decided on
evaluating tbe effect of tbyme essence on biocbemical factors sucb as {triglyceride, cbolesterol, HDL, LDL,
ALT, AST and total protein] in rat's blood serum. Tbyme essences in 200 and 400 mgJhg levels were
prescribed respectively for animals in two treatment group tbrougb oral administration during 21 days
and tbe tbird group as control received no compositions. It was observed tbat after measuring bio-
cbemical factors of blood serum tbe tbyme essences led to decrease cbolesterol level significantly in botb
treatment groups compared witb control group {p< 0.05].
Submitted: May, 2014
Revised: May,2014
Accepted: May, 2014
Key worJx:
Tbyme;
Tbyme vulqoris l.;
Biocbemicol foctors;
Serum;
Rots
INTRODUCTION
* Correspondence to: Mobsen JaIarIan DebkordI, Department oI PatboIogy, FacuIty oI VeterInary MedIcIne,
Sbabrekord Brancb, IsIamIc Azad UnIversIty, Sbabrekord, Iran
EmaII: m.jaIarIandyaboo.com; Pbone no - 09S-913-2S0-6S14
Inteinational 1ouinal of
ife Sciences
L
Iounded 2OO7
Copyr|ght Internot|ono| Journo| o| L||e Sc|ences
Copyr|ght reserved Internot|ono| Journo| o| L||e Sc|ences Do|:dx.do|.orgJ10.312J|j|s.v8|3.10433
Internot|ono| Journo| o| L||e Sc|ences Internot|ono| Journo| o| L||e Sc|ences
Thyme is an aiomatic plant of Lamiaceae family, founu
in the mountainous iegion of Iian anu has meuicinal
uses. Its essence has antifungal activity anu iemoves
Asperqillus fungi the cause of aflatoxin, anu show
effective woik on bovine iespiiatoiy uisease (Katooli et
al., 2u12; Aizani et al., 2u1S). All of the Thyme is
eniicheu with volatile compounus which mainly contain
thymol anu caivaciol that aie consiueieu as stiong
antiseptic (Shati anu Elsaiu, 2uu9; Bajimehuipooi et al.,
2u1u). The shoots (aeiial oigans) act as antiseptic
expectoiant anu aie useu to tieat chionic chest pain. It
is helpful to tieat inuigestion anu uiaiihea too (Aizani
et al., 2u1S; Kensaia et al., 2u1S). This meuicinal plant is
iecommenueu to tieat iheumatism, muscle stiains,
insect bites, uisinfecting wounu, chionic cough, lung
uiseases, soie thioat, anu iiiitateu stomach bowels.
Piegnant women aie auviseu not to use this heib anu
ISSN 201-0525
Cite tbis article as -
Doi:dx.doi.orgJ
Debhordi, M. ), Debhordi, H. S., Mobaraheb, H. I., Kbamesipour, F., Hamedi, B. {2014] Assessing tbe effect of tbe tbyme {1hyme vulgurlx
L.] essence on some biocbemical factors in rat's blood serum. Internutlonul journul of Ilfe Sclencex 8{3]: 15 - 18.
10.312Ji|ls.v8i3.10433
MATERIALS & METHODS
also it is bettei to use uiluteu foim of plant because the
thickei foim may iiiitate mucosal tissues anu skin
(Fachini-Queiioz et al., 2u12; Kensaia et al., 2u1S).
Thyme has anticoagulation effect anu pievents platelets
accumulation stiongly thiombosis anu atheioscleiosis
(Al-Khalaf, 2u1S). Its infusion as 1ug leave powuei in 1
litei watei is useu to tieat lung uiseases as 2Sg pei litei
with a little honey to tieat uigestive system uisoiueis
(Fachini-Queiioz et al., 2u12; Al-Khalaf, 2u1S; Kensaia
et al., 2u1S).
In piesent stuuy we aimeu to evaluating the effect of
thyme essence on biochemical factois such as (tiigly-
ceiiue, cholesteiol, BBL, LBL, ALT, AST anu total piot-
ein) in iat's bloou seium.
S1udg ond somp1!ng
This stuuy was conuucteu in 2u1S in the Islamic Azau
0niveisity of Shahiekoiu Bianch laboiatoiy. The bio-
TabIe 1. Tbe eIIect oI tbe tbyme (Tgme vu1gor!s L.) essence
on cboIesteroI IeveI
1
chemical factois weie measuieu at Al-Nahui Neuical
Biagnostic Laboiatoiies of Shahiekoiu, Iian. The
thyme plant tabs weie collecteu anu the extiact of
ielateu plant was piouuceu in the Islamic Azau
0niveisity of Shahiekoiu Bianch laboiatoiy.
The thyme plants weie collecteu fiom Iian anu they
weie accuiately iuentifieu anu confiimeu by means
of auapting the heibaiium specimens. Then they
weie uiieu at 2S-SS C tempeiatuie foi S houis. Aftei
uiying they weie ciusheu anu extiacteu using BP
(Biitish Phaimacopoeia, 1988). It shoulu be noteu
that 1 cc essence weie obtaineu pei 1uu gi plant.
Drg!ng ond e1roo1!ng
In piesent stuuy 24 seiies of white wistai iats piepaieu
fiom Islamic Azau 0niveisity of Shahiekoiu Bianch
laboiatoiy animals bieeuing centei weighteu 21S1S
gi weie kept in stanuaiu cages with easy access to
watei anu foou. The iats weie uiviueu into S eight -
gioups. In the fiist anu seconu gioup the ueteimin-
ation of essence uose was caiiieu out thiough pieli-
minaiy expeiiments anu no composition was useu foi
contiol gioup. The fiist anu seconu gioup ieceiveu
2uu anu 4uu mgkg savoiy uose iespectively while
contiol useu steiile uistilleu watei. Beibal piesciiption
lasteu foi 21 uays among gioups anu at the enu of this
peiiou the animals weie anesthetizeu in the specific
containeis using chloiofoim. The bloou samples weie
taken by means of caiuiac punctuie technique (Sumiko
et al., 2uu1) anu theii seiums was isolateu via 2uuu
ipm centiifuge anu finally biochemical factois such as
tiiglyceiiue, total piotein, cholesteiol, BBL, LBL, ALT,
anu AST anu weie measuieu. All expeiiments weie
caiiieu out unuei ethical guiuelines of the Islamic
Azau 0niveisity of Shahiekoiu Bianch, foi the caie
anu use of laboiatoiy animals.
An!mo1s
The uata was analyzeu statistically by means of SPSS
softwaie 18.u (SPSS Inc., Chicago, IL, 0SA) anu signif-
icance levels (p<u.uS) weie compaieu by means of
Buncan test.
S1o1!s1!oo1 Ano1gs!s
RESULTS
The level of seium tiiglyceiiue in the fiist gioup was
121.298.7 mgul which showeu no significant
uiffeience (p<u.uS) compaieu to contiol gioup
(111.721 mgul). Also the level of seium tiiglyceiiue
in the seconu gioup was SS.78.4 mgul which
showeu no significant uiffeience (p<u.uS) compaieu
to contiol gioup (111.721 mgul) (Table 1;
Figuie 1). The level of total piotein in the fiist gioup
was 6.S1. 6 mgul which showeu no significant
uiffeience in compaiison to contiol gioup (7.S 1.7
mgul). The iatio of total piotein in the seconu gioup
was 7.4u.71 mgul which showeu no significant
uiffeience in compaiison to contiol gioup but total
piotein ieuuction ielatively in both tieatment gioups
in compaiison to contiol gioup (Table 1; Figuie 2).
The level of LBL in the fiist gioup was S.SS.S mgul
which showeu no significant uiffeience in compaiison
to contiol gioup (4.S1 mgul). LBL level in the
seconu gioup was 12.1S.8 mgul which showeu
significant uiffeience compaieu with contiol gioup
(4.S1 mgul) (Table 1; Figuie S). The level of BBL in
the fiist gioup was 9.21.9 mgul which showeu no
significant uiffeience in compaiison to contiol gioup
(121.4 mgul). The iatio of BBL in the seconu gioup
was 11.Su.S8 mgul which showeu no significant
uiffeience in compaiison to contiol gioup (121.4 mg
ul) but BBL iatio showeu a ielative ieuuction gioups
in compaiison to contiol gioup (Table 1; Figuie 4).
In the fiist gioup the level of cholesteiol was 64.7
12.6 mgul which showeu ieuuction significant
uiffeiences than contiol gioup (142.224.1S mgul).
The cholesteiol in the seconu gioup was at 6S.S1S.S2
mgul level that showeu significant uiffeiences
(p< u.uS) than contiol gioup (142.224.1S mgul)
(Table 1; Figuie S). The piopoition of ALT in the fiist
gioup was S9.S 17.4 mgul which showeu no signifi-
cant uiffeiences than contiol gioup (8u1u.1 mgul).
The piopoition of ALT in the seconu gioup was 92.7
S8.S mgul which showeu no significant uiffeiences
than contiol gioup (8u1u.1 mgul). Bowevei, both
tieatments gioups showeu a ielative inciease than
contiol gioup (Table 2; Figuie 6). The piopoition of
AST in the fiist gioup was 12644 mgul which
showeu no significant uiffeiences than contiol gioup
(17S.7SS.8 mgul). The piopoition of AST in the
seconu gioup was 12414.S mgul which showeu no
significant uiffeiences than contiol gioup (17S.7SS.8
mgul). Bowevei, both tieatments gioups showeu a
ielative ieuuction than contiol gioup (Table 2; Figuie 7).
uioups
T. vulqoris l.
essence was
useu
Total
piotein
Cholesteiol Tiiglyceiiue LBL BBL
Contiol -
7.Su
1.7
a
142
24.1S
a
111.7
21
a
4.Su
1
a
12
1.4
a
uioup 1 2uu mgkg
6.Su
1. 6
a
64.7u
12.6
b
121.2
98.7
ab
S.Su
S.S
ab
9.2u
1.S
a
uioup 2 4uu mgkg
7.4u
u.71
a
6S.Su
1S.S
b
S1.7
8.4
b
12.1u
S.8
b
11.Su
1.S8
a

In eacb column tbe numbers witb similar letter bave no significant differences {p< 0.05].
TabIe 2. Tbe eIIect oI tbe tbyme (Tgme vu1gor!s L.) essence
on IIver enzymes oI rat bIood serum

uioups
Tbyme vulqoris l.
essence was useu
ALT AST
Contiol -
8u
1u.1
a
17S.7
SS.8
a
uioup 1 2uu mgkg
S9.S
17.4
a
126
44
a
uioup 2 4uu mgkg
92.7
S8.S
a
124
1S.S
a

In eacb column tbe numbers witb similar letter bave no significant differences {p< 0.05].
I n t e r n o t | o n o | J o u r n o | o | L | | e S c | e n c e s 8 [ 3 } : 2 0 1 4 ; 1 5 - 1 8
DISCUSSION
This manusciipt aims to assess the effect of the
'thyme' essence on levels of biochemical factois in iat
bloou. Accoiuing to the Table 1 the tieatment gioups
uiffeieu significantly fiomcontiol gioup as the seconu
gioup hau moie ieuuction than the fiist one which
can be because of ieceiving moie thyme essence uose.
Besiues the thyme essence in 4uu mg uose leu to
ieuuction of tiiglyceiiue level significantly. Beiiis et
al. uemonstiateu that some meuicinal heibs playeu a
iole in inhibiting action of lipopioteins glicacion,
enzymes, anu pioteins involveu in lipiu anu lipo-
pioteins metabolism so that cause seium lipiu
ieuuction (Baiiis et al., 2u11). Nany ieseaicheis
inuicateu that some plants with natuial antioxiuants
such as likopen, vitamin E anu phenols iesulteu in
seium lipiu ieuuction (}iang anu Busting, 2uuS).
Sahin anu kukuk showeu that vitamin E- eniicheu
uiets anu some phenolic compounus of meuicinal
plants can enfoice antioxiuant uefense system anu
holu back fiee iauicals foimation within bouy anu
ieuuce oxiuation (Sahin anu Kucuk, 2uuS). Theii
iesults aie paiallel to the iesults of piesent stuuy as
thyme with phenolic composites ieuuceu the seium
cholesteiol significantly. Rauvan et al founu that
thyme ieuuceu malonuialuehyue compaieu to contiol
gioup. They suggesteu that thyme can be consiueieu
as an appiopiiate antioxiuant (Rauwan et al., 2uu8).
In piesent stuuy thyme causeu a ielative cholesteiol
ieuuction (LBL) in the fiist gioup with 2uu mgkg
thyme essence which this ieuuction can be attiibuteu
to the antioxiuant effect of thyme plant. Smaith et al
ievealeu that the thyme leu to glutathione ieuuctase
enhancement in livei iat feeu with it anu finally
iesulteu in ieuucing piouuction of fiee iauicals
(Smaith et al., 2uu8). Some ieseaich which stuuieu
anticanuiual activity of thymol (effectiveness mateiial
I n t e r n o t | o n o | J o u r n o | o | L | | e S c | e n c e s 8 [ 3 } : 2 0 1 4 ; 1 5 - 1 8
17

FIgure 1. Tbe eIIect oI tbyme essence IeveI oI trIgIycerIde oI rat bIood serum;
FIgure 2. Tbe eIIect oI tbyme essence IeveI oI totaI proteIn oI rat bIood serum;
FIgure 3. Tbe eIIect oI tbyme essence IeveI oI LDL oI rat bIood serum;
FIgure 4. Tbe eIIect oI tbyme essence IeveI oI HDL oI rat bIood serum;
FIgure S. Tbe eIIect oI tbyme essence IeveI oI cboIesteroI oI rat bIood serum;
FIgure 6. Tbe eIIect oI tbyme essence IeveI oI ALT oI rat bIood serum;
FIgure ?. Tbe eIIect oI tbyme essence IeveI oI AST oI rat bIood serum.
1 2 3
4 5
7
Abu El Kauei NA anu Nohameu NZ (2u12) Evaluation of Piotective anu Antioxiu-
ant Activity of Thyme (Thymus vulgaiis) Extiact on Paiacetamol-Inuuceu Toxicity
in Rats. Austialian }ouinal of Basic anu Applieu Sciences 6(7): 467-474.
Al-Khalaf NI (2u1S) Thyme anu Thymol Effects on Inuuceu Bionchial Asthma in
Nice. Life Science }ouinal 1u (2): 69S- 699.
Aizani B, Notameui Nanu Aizani A (2u1S) Chemical Compounus of Thyme as a
Neuicinal Beib in the Nountainous Aieas of Iian. The }ouinal of Nutiitional Biso-
iueis & Theiapy S12: uuS. uoi:1u.41722161-uSu9.S12-uuS.
Bahiami N, Shaiiatmauaii N, Kaiimi F anu Toishizi NA (2u11) Effect of uietaiy
extiact of Thyme anu Peppeimint anu vitamin E supplementation on immune
iesponses of laying hen in heat stiess anu content of peioxiuation egg uuiing sto-
iage. Iianian }ouinal of Neuicinal anu Aiomatic Plants 27 (2): S26-SS7.
Biitish Phaimacopoeia. 1988. Biitish phaimacopoeia, vol. 2. BNS0, Lonuon, pp.
1S71S8.
Fachini-Queiioz FC, Kummei R, Estevao-Silva CF, ue Baiios Caivalho NB, Cunha
}N, uiespan R, Beisani-Amauo CA anu Cuman RKN (2u12) Effects of Thymol anu
Caivaciol, Constituents of Thymus vulgaiis L. Essential 0il, on the Inflammatoiy
Response. Eviuence-Baseu Complementaiy anu Alteinative Neuicine volume 2u12,
Aiticle IB 6S7u26, 1u pages uoi:1u.11SS2u126S7u26.
uanong WF (2uuS) Review of meuical physiology 22 euition. NCuRAW. Bill Comp-
anies,Inc.
uuyton AC anu Ball }E (2uu6) text book of meuical physiology. 11 euition, Elseviei
Inc. SS9-SS6 .
Bajimehuipooi B, Shekaichi N, Khanavi N, Auib N anu Amii N(2u1u) A valiuateu
high peifoimance liquiu chiomatogiaphy methou foi the analysis of thymol anu
caivaciol in Thymus vulgaiis L. volatile oil. Phaimacognosy Nagazine 6 (2S): 1S4-
1S8. uoi: 1u.41uSu97S-1296.66927.
Baiiis CS, Beaulieu LP, Fiasei NB, NcIntyie KL, 0wen PL, Naitineau LC, Cueiiiei
A, }ohns T, Bauuau PS, Bennett SA anu Ainason }T (2u11) Inhibition of auvanceu
glycation enu piouuct foimation by meuicinal plant extiact coiielates with phen-
olic metabolites anu antioxiuant activity . Planta Neuica 77 (2): 196-2u4 .
}iang F, Busting u} (2uuS) Natuial phenolic compounu as caiuiovasculai theiape-
utics: potential iole of theii anti-inflammatoiy effects. Cuiient vasculai Phaimac-
ology 1 (2): 1SS-1S6 .
Katooli N, Naghsoulo R, Bonaii B anu Razavi SE (2u12) Fungistatic activity of Ess-
ential oil of Thyme anu Eucalyptus against of Posthaivest anu soilboine Plant Pat-
hogenic fungi. ulobal }ouinal of Neuicinal Plant Reseaich 1(1): 1-4.
Kensaia 0A, ElSawy NA, El-Shemi Au anu Beauei EA (2u1S) Thymus vulgaiis sup-
plementation attenuates bloou piessuie anu aoita uamage in hypeitensive iats.
}ouinal of Neuicinal Plants Reseaich 7 (11): 669-676.
0mian SN, Esmaeilzaueh S anu Rahmani Z (2u1u) Laboiatoiy stuuy of anticanui-
ual activity of thyme, pennyioyal anu lemon essential oils by micio uilution meth-
ou. }unuishapui }ouinal of Niciobiology S(4): 161-167.
Rauwan NL, Bassan RI, Qota ENanu Fayek BN(2uu8) Effect of natuial anti oxiu-
ant on oxiuative stability of eggs anu piouuctive anu piepiouuctive peifoimance
of laying Bens. Inteinational }ouinal of Poultiy Science 7 (2): 1S4-1Su.
Sahin K anu Kucuk 0 (2uuS) Beat stiess anu uietaiy vitamin supplementation of
poultiy uiets . Nutiition abstiact anu ieviews seiies B: live stock feeus anu feeuing
7S (7): 41-Su.
Shati AA anu Elsaiu Fu (2uu9) Effects of watei extiacts of thyme (Tbymus vulqoris)
anu gingei (Zinqiber officinole Roscoe) on alcohol abuse. Foou anu Chemical Toxic-
ology 47: 194S1949.
Shittu 0K, 0mai B anu 0sman 0Y (2u1S) Effect of methanolic leaf extiact of
Tbymus vulqoris on some biomaikei enzymes in Tiypanosoma biucei infecteu iats.
Inteinational }ouinal 0f Phaimaceutical Anu Biomeuical Reseaich 4(2): 8S-87
Smaith RN, Panwai N, Kumai N, Soni I, Kumai Nanu Kumai I (2uu8) Evaliuation
of antioxiuant anu iauical scavemging activities of ceitain iauiopiotective plant
extiacts . Foou Chemistiy 1u6 (2): 868-87S .
Sumiko N, Cisinta FN, uuileimo RN, Nestoi NP anu vicente BS (2uu1) Testosteione
effect on insulin content, messengei iibonucleic aciu lvel, Piomotei activity anu
seciation in iat. Enuociinology 142: 144- 147.
REFERENCES
Submit your next manuscript to I)LS witb a -
1. Convenient online submission,
2. Rapid editorial reviewfollowed by peer review,
3. Immediate publication on acceptance.
International )ournal of Life Sciences
NUTE: AutborsJcontributors are responsible
for originality, contents, correct references, and
etbical issues.
of thyme) (0mian et al., 2u1u). The Abu El Kauei anu
Nohameu stuuy (2u12), suggests that the aqueous
thyme extiact has a tiemenuous potential to pievent
oi ieveise the changes inuuceu by paiacetamol
toxicity back to noimal via its antioxiuant activity
(Abu El Kauei anu Nohameu, 2u12). Bahiami et al
(2u11) stateu that phenolic compounus incluueu in
thyme can leu to inciease the efficiency of enzymes
involveu in the antioxiuant uefense system anu boost
bouy's immune cells (Bahiami et al., 2u11). In anothei
stuuy, inuicates that thyme anu thymol incieaseu the
iates of antioxiuants in the bouy, anu the ability to get
iiu of oxiuative agent anu fiee iauicals that aie
geneiateu insiue the bouy, oi uue pollution
enviionment. Bence, this stuuy confiims the potential
effect of both thyme anu thymol as possible means to
tieat asthma (Al-Khalaf, 2u1S). Katooli et al. (2u12)
showeu that uemonstiateu the antifungal activity of
essential oil of Thyme against phytopathogenic fungi
in vitio (Katooli et al., 2u12). Thymus vulgaiis
methanolic leaves extiact has tiypanociual piopeities
anu incieases the seiumanu livei specific activities of
some biomaikeis, which might be uue to inteiaction
between infective conuition anu the constituents of
the extiacts (Shittu et al., 2u1S). supplementation
with T. vulgaiis as an heibal iemeuy has shown
iemaikable antihypeitensive effect anu maikeu
impiovement on hypeitension-ielateu biochemical
changes anu aoitic vasculai uamage in iats (Kensaia
et al., 2u1S). Accoiuing to the Table 1 total piotein
iatio in the fiist tieatment with 2uu mgkg uose
ieuuceu compaieu to contiol gioup but no
significantly that was moie in the seconu gioup
compaieu to the fiist one. The ieason of ieuuction
total piotein ieuuction may explain the ieuuction of
bloou buffei capacity anu colloiual osmotic piessuie
capacity which iesult in fluiu loss in capillaiies since
plasma piotein is iepoiteu as a 1S% iesponsible foi
bloou buffei capacity (uanong, 2uuS) anu plasma
pioteins inuuceu osmotic piessuie (which calleu
colloiual osmotic piessuie) uesiie to move fluiu using
osmotic piessuie to the intei-tissue space (uuyton
anu Ball, 2uu6).
CONCLUSION
Consequently, the essence of thyme leu to ueciease
bloou seium cholesteiol significantly. Also its effect
on LBL anu Tiig factois is uose-uepenueu, since LBL
iatio in the seconu gioup with highei thyme (4uumg
kg) incieaseu significantly meanwhile in the fiist
gioup with lowei essence (2uumgkg) it wasn't
significant. Foi this ieason it can be saiu that thyme
in 2uumgkg uose hau antioxiuant activity. Fuithei-
moie the Tiig level in the seconu gioup with highei
essence (4uumgkg) uecieaseu significantly but in
the gioup with lowei uose showeu a ielative
inciement.
I n t e r n o t | o n o | J o u r n o | o | L | | e S c | e n c e s 8 [ 3 } : 2 0 1 4 ; 1 5 - 1 8
18