MicroRNA Scientic Interest

Group
University of California San Diego
Erick Tatro
etatro@ucsd.edu
First Meeting
October 14, 2014

4:00 - 4:05 Introduction

4:05 - 4:20 Study of MicroRNA and Memory

Decits in Aging

4:20 - 4:50 Sketches of miRNA Regulation in T

and B Cells: Prospects for
Immunogenomic Therapy

4:50 - 5:00 Discussion

AGENDA
MicroRNA and Memory Decits in
Aging
Erick T. Tatro
Department of Psychiatry, UC San Diego
October 14, 2014

Sam and Rose Stein Institute for Research on Aging

Young vs Aged Mouse

Some Aged Mice Exhibit Memory Decit

BACKGROUND
Model Mammal
O
b
j
e
c
t

D
i
s
c
r
i
m
i
n
a
t
i
o
n

R
a
t
i
o

(
D
R
)
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
O
b
j
e
c
t

D
i
s
c
r
i
m
i
n
a
t
i
o
n

R
a
t
i
o

(
D
R
)
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
Young Old
Impaired Normal Normal
Aged
Training Phase
Testing Phase
?
Mouse - TaqMan Low Densi ty Array (384 wel l s)
MIRNA ARRAY
Hippocampus
User Guide
TaqMan

Array Micro Fluidic Cards

L
o
g

(
F
o
l
d

C
h
a
n
g
e
)
-0.4
-0.3
-0.2
-0.1
0.0
0.1
0.2
0.3
0.4
Young Unimpaired Aged Impaired Aged
Group
Legend
miR-130b
miR-138
miR-222
miR-29b
miR-339-3p
miR-340-5p
miR-582-3p
Young Aged
Normal Normal Impaired
L
o
g

(
F
o
l
d

C
o
n
t
r
o
l
)

E
x
p
r
e
s
s
i
o
n
Tatro & Scott et al. PLOS One (2010)
Validate & Image
RESULTS
Q PCR
L
o
g
(
m
i
R
-
1
3
8
)
-1.0
-0.5
0.0
0.5
1.0
Group
Young
Aged Unimpaired
Aged Impaired
Young Old
Impaired Normal Normal
In Situ Hybridization
Mi R-138
Whole Brain
Hippocampus
Whole Brain
Hippocampus
Young
Aged /
Impaired
Mi R-138
Aged
Exiqon Corporation
Target of miR138
SIGNIFICANCE
S
SH
APT1
APT1
mRNA
miR-138
Palmitoylation of Proteins in Dendritic Spine
Stable
Extend
Acyl Protein Thioesterase 1
Target of miR138
RESULTS
A
P
T
1
-
m
R
N
A
-0.25
0.00
0.25
0.50
0.75
1.00 Group
Young
Aged Unimpaired
Aged Impaired
Young Aged
Normal Normal Impaired
Q PCR In Situ Hybridization
Acyl Protei n
Thi oesterase
Acyl Protei n
Thi oesterase
Whole Brain
Dentate Gyrus
Hippocampus
Aged
Serial Sections
RESULTS
miR-138 APT1 Protein
Dentate
Gyrus
Aged /
Impaired
Young
Tatro et al. Am J Geriatric Psych. (2013)
CONCLUSION

Age associated memory impairment correlated

with lower miR-138 expression, particularly in
the hippocampus.
FURTHER STUDY

Examine miR - mediated syanptodendritic

changes.

Examine causes of differential miR-138

regulation: epigenetics, transcription factors,
HPA axis homeostasis.
THANK YOU

Department of Psychiatry

Cristian Achim, Ian Everall, Mariana Cherner, Svetlana Semenova, David Moore, Igor
Grant, Hamp Atkinson

Eliezer Masliah, Erick Scott

Ben Soontornniyomkij, Virawudh Soontornniyomkij, Silke Nuber, Ben Guoux, Michael

Yang, Intan Purnajo, Alex Yermanos

Sam and Rose Stein Institute for Research on Aging

Dillip Jeste, Vicki Risbrough, Jared Young

UC San Diego Center for AIDS Research

Virology Core

Davey Smith, Sara Gianella, Doug Richman

Genomics Core

Christopher Woelk, Steffney Rought, Pinyi Du

HIV Neurobehavioral Research Programs

Exiqon Corporation (Small Grants Award for Supplies)

NIDA (R03DA031591) and NIMH

4:00 - 4:05 Introduction

4:05 - 4:20 Study of MicroRNA and Memory

Decits in Aging

4:20 - 4:50 Sketches of miRNA regulation in T and

B cells: Prospects for immunogenomic
therapy

4:50 - 5:00 Discussion

AGENDA
MicroRNA Scientic Interest
Group
University of California San Diego
Erick Tatro
etatro@ucsd.edu
First Meeting
October 14, 2014