VINEET CHOPRA 5, Bankers Colony, Gumanpura,

vineetpchopra@gmail.com Kota, Rajasthan

+919261345470, +919529532375



Objective

To enhance my skills in a work related environment and learn new things that will make me a better
professional. Use my talents by putting them into good use for the benefit of the organization that I
work for.

Academic background

Examination Specialization University Year CPI/ %
Post graduation Biotechnology IIT Bombay 2011 7.87

Graduation Biotechnology M.G.S. university 2009 75

Intermediate Board of Secondary
Education, Rajasthan
2003 63

Academic achievement

Qualified in GATE(Graduate aptitude test of Engineering) organized by IIT (2010)
Secured AIR - 23 in IIT-JAM (2009)
Secured AIR - 124 in JNU - CEE (Combined entrance exam for M. Sc.
Biotechnology) (2009)

Academic projects and seminars

M. Sc. Project: Purification and characterization of carbaryl degrading enzyme carbaryl
hydrolase from Pseudomonas sp. strains C4 & C5
The enzyme carbaryl hydrolase was purified and characterized from both strains
Guide: Prof. P. Phale, Dept. of Biosciences & Bioengineering, IIT Bombay (2010-11)
Certification course on Fundamentals of Intellectual property rights (IPRs): patents,
copyright, trademark, trade secrets, trade dress, designs etc at IIT Bombay (2011)
Paper presentation on Kinetics of BSA-Bilirubin binding and effect of palmitate by
Spectrophotometry, IIT Bombay (2010)
Presented a seminar on Cell immobilization and its applications, IIT Bombay (2009)
Presented a seminar on industrial applications of bacteria Aeromonas caviae (2010)

Technical Skills

Biochemistry & Instrumentation: Protein concentration determination (Lowry, Bradford,
UV-visible Method), Ion-exchange chromatography, Hydrophobic chromatography, Gel
filtration chromatography, Affinity chromatography, Thin Layer Chromatography (TLC),
Enzyme assay, Enzyme kinetics
Molecular biology Techniques: Polymerase chain reaction (PCR), Plasmid based cloning,
Cloning into bacterial expression and phage vectors, Restriction analysis of Plasmid DNA,
Plasmid isolation, Agarose Gel Electrophoresis, SDS-PAGE, Isolation of Genomic DNA,
Bacterial transformation
Microbiological techniques: Growth curve, Staining techniques, Sterilization techniques,
Biochemical identification of Microbes, Antibiotic assay, replica plating etc
Bioinformatics tools and techniques: Multiple Sequence alignment, Sequence analysis,
BLAST, FASTA, Hidden Markov models; Structure prediction methods (homology),
Substitution matrix
Computer Skills: Windows XP/08/00/07/Vista OS, Microsoft office (Word, Excel, Power
point)


Laboratory Project

Microbiology laboratory project- Isolation, identification and characterization of an
unknown bacterium from sewage water sample.
Analytical biochemistry laboratory project- GDH protein purification from bacterial
culture.
Biophysics laboratory project- Protein-Ligand binding assays, Protein folding-unfolding
studies.
Genetic engineering laboratory project- Cloning, transformation and expression of Cst
gene in E.coli DH5 and purification of Cst gene product using affinity column
chromatography

Research Experience

Worked as Project assistant in metabolic engineering lab in Dept. of Biosciences and
Bioengineering at IIT Bombay (Feb 2011 June 2011)

Kinetic characterization of enzyme carbaryl hydrolase obtained from Pseudomonas
sp. Strain C4 & C5 in terms of kinetic constant Km and pH optimum.

Enzymes sensitivity towards various metal ions was studied.

M.Sc. Dissertation (May 2010 Nov. 2011): Purification and characterization of enzyme
carbaryl hydrolase from carbaryl degrading Pseudomonas sp. strain C4 & C5

Carbaryl is an insecticide and is a member of carbamate family of pesticide. It is one
of the most widely used insecticides in agricultural fields. It is an inhibitor of acetyl
choline esterase causing toxic effects on exposure.

Carbaryl hydrolase was partially purified using various chromatographic techniques
such as ion-exchange, hydrophobic and gel filtration and ammonium sulphate
precipitation etc.

Molecular mass of the enzymes were determined with the help of SDS-PAGE
calibration curve and Sephacryl S 200 gel filtration curve.

Positions of responsibility

Generations 2010: Contributed to organization of national level festival organized by
Symbiotek Bioschool Association, IIT Bombay (2010)
GeneRations 2011: Worked as finance coordinator, leading the team to attract sponsors
for the event (2011)



Personal Details

Date of birth: 23 December 1985
Marital Status: Single
Languages: English, Hindi
Home-town: Bikaner (Rajasthan)
Permanent Address: c/o: C. L. Chopra, Gandhi Chowk, New Lane, Gangashahar, Bikaner-
334401, Rajasthan
Interested in cricket, carom, computers and crosswords