Store at 20C

Paclitaxel
31 mg
n

#9807

Orders n 877-616-CELL (2355)

orders@cellsignal.com
Support n 877-678-TECH (8324)
info@cellsignal.com
Web n www.cellsignal.com
rev. 07/13/09

This product is for in vitro research use only and is not intended for use in humans or animals.
This product is not intended for use as a therapeutic or in diagnostic procedures.

Background: Paclitaxel belongs to the taxane family

of antitumor and antileukemic agents (3). By binding to
-tubulin and promoting the assembly of microtubules,
paclitaxel prevents microtubual depolymerization and blocks
normal cell division (13). The microtubule dysfunction
induced by paclitaxel results in aberrant cell mitosis and
sometimes apoptosis. The IC50 of paclitaxel-induced mitotic
block is 4 nM (4).

kDa

Storage: Store tightly sealed at 20C.

200

Please visit www.cellsignal.com for a complete listing

of recommended companion products.

140
100
80

Phospho-cdc25C
(Thr48)

Molecular Formula: C47H51NO14H2O

60
O

50
O
C6H5

200

OH
H5C6

N
H

140

OH

HO

100
O

H5C6
O

80

cdc25C

60

Molecular Weight: 853.92 Da

0.001

0.01

0.1

50

Directions for Use: Paclitaxel is supplied as 1 mg powder. Store at or below -20C. Before use, dissolve powder in
1.15 ml DMSO to make a 1 mM paclitaxel stock solution.
For working concentrations of 100 nM-1000 nM, dilute
DMSO stock 1:10,000 to 1:1000. Treat cells with the desired
concentration for 6-48 hours. Paclitaxel is more stable as
a powder than in solution; it is therefore recommended to
dissolve as close as possible prior to use. Store solution at
or below -20C.

Paclitaxel (M)

Western blot analysis of extracts from HT29 cells, either untreated or treated with indicated concentration of paclitaxel using
Phospho-Cdc25C (Thr48) Antibody #9527 (upper) or control
Cdc25C (5H9) Rabbit mAb Antibody #4688 (lower).

Background References:
(1) Rowinsky, E.K. et al. (1990) J. Natl. Cancer Inst. 82,
12471259.
(2) Jordan, A. et al. (1998) Med. Res. Rev. 18, 259296.
(3) A bal, M. et al. (2003) Curr. Cancer Drug Targets 3,
193203.

2007 Cell Signaling Technology, Inc.

(4) Long, B.H. and Fairchild, C.R. (1994) Cancer Res. 54,
43554361.

Applications Key:

WWestern

Species Cross-Reactivity Key:

IPImmunoprecipitation
Hhuman

Mmouse

Dgdog Pgpig ScS. cerevisiae Allall species expected

IHCImmunohistochemistry
Rrat

Hmhamster

ChIPChromatin Immunoprecipitation

Mkmonkey

Mimink

Cchicken

IFImmunofluorescence

FFlow cytometry

DmD. melanogaster XXenopus

Species enclosed in parentheses are predicted to react based on 100% homology.

Zzebrafish

E-PELISA-Peptide
Bbovine

#9807 MSDS

Material Safety Data Sheet (MSDS) for

Paclitaxel

I. Identification:

VIII. Exposure Controls/Personal: Occupational exposure

limits not found.

Ventilation System: A system of local and/or general exhaust is recommended.
Skin Protection: Wear compatible chemical resistant gloves and protective clothing.
Eye protection: Wear protective safety glasses. Maintain eye wash fountain and quick-drench
facilities in work area.

Product name: Paclitaxel

Product Catalog: 9807
CAS number: Not applicable to mixtures
Manufacturer Supplier: Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923 USA
978-867-2300 TEL
978-867-2400 FAX
978-578-6737 EMERGENCY TEL

IX. Physical And Chemical Properties

Appearance:
Color:
Odor:
Boiling Point:
Melting Point:
Ignition temperature
Volatile Organic Compounds
pH
Solubility:

II. Composition/Information:
Substance Name:
Synonyms:
CAS#:
Molecular Weight:
Molecular Formula:

Paclitaxel
Taxol
33069-62-4
853.92
C47H51NO14

III. Hazard Identification:

To the best of our knowledge, the chemical, physical, and toxicological properties of this solution have not been thoroughly investigated.
RTECS: DA8340700
EMERGENCY OVERVIEW: May be an irritant. May cause irritation to eyes, skin, mucous
membranes.
HMIS rating:
Health 1

Flammability 0

Reactivity 0

IV. First Aid Measures:

X. Stability and Reactivity:

Stability: Stable under recommended storage conditions.
Conditions to avoid: Moisture. Protect from light and heat.
Materials to avoid: Avoid acids and bases.
Hazardous Decomposition: Carbon oxides, sulfur oxides.
Hazardous exothermic reactions: During fire conditions may produce toxic gases such as
carbon monoxide, carbon dioxide.

XI. Toxicological Information: To the best of our knowledge, the

chemical, physical, and toxicological properties of this solution have not been thoroughly
investigated.

Inhalation: If inhaled, remove to fresh air. If breathing is difficult, get medical attention.
Ingestion: If swallowed, wash out mouth with water provided person is conscious. Get
medical attention.
Skin exposure: In case of contact, immediately wash skin with soap and water for at least 15
minutes. Remove contaminated clothing. Wash clothing before reuse.
Eye exposure: In case of contact with eyes, immediately flush eyes with water for at least 15
minutes. Get medical attention.

RTECS # DA8340700

V. Fire Fighting Measures:

XIII. Disposal Considerations: Dispose of in accordance with

Flash Point: Data not available.

Autoignition Temperature: Data not available.
Fire extinguishing media: Water spray, dry chemical, foam, or carbon dioxide.
Firefighting: Wear protective clothing and self-contained breathing apparatus to prevent
contact with skin and eyes.

VI. Accidental Release Measures: Take appropriate precautions

to minimize direct contact with skin, eyes, mucous membranes, and prevent inhalation of dust.
Isolate the spill area. Wear approved self-contained breathing apparatus, protective clothing and
chemical resistant gloves. Sweep up carefully to avoid raising dust and transfer to a chemical
waste container for disposal according to local, state, and federal regulations. Ventilate area and
wash spill site after material pickup is complete. Do not let substance enter drains.
2008 Cell Signaling Technology, Inc.

crystalline powder
white
none
data not available
210-220C
data not available
data not available
data not available
not soluble in water; soluble in DMSO, alcohols,
and chlorinated solvents

Acute Effects: Data not available.

Primary Effects: Data not available. May be an irritant to eyes, skin, respiratory system.
Chronic Effects: Data not available.

XII. Ecological Information: Data not available for this product.

federal, state, local environmental regulations.

XIV. Transport Information:

DOT: Proper Shipping Name: None.

This substance is considered non-hazardous for transport.
IATA: Proper Shipping Name: None.
This substance is considered non-hazardous for air transport.

XV. Regulatory Information:

VII. Handling And Storage:

EU Regulations/Classifications/Labeling Information: Not available for this product.

US Regulatory Information:

Not SARA listed.

Avoid contact with skin, eyes, mucous membranes and respiratory system. Wash hands
thoroughly after handling.

XVI. Other Information:

Storage: Store in tightly closed container at -20C.

Orders n 877-616-CELL (2355)

orders@cellsignal.com

This product is not intended for use in humans. To the best of our knowledge, this document is
accurate. It is intended to serve as a guide for safe use of this product in a laboratory setting by
experienced personnel. The burden of safe use of this material rests entirely with the user. The
above information is believed to be accurate but is not necessarily all-inclusive and shall be
used only as a guide. Cell Signaling Technology, Inc., shall not be held liable for any damage
resulting from the handling of or from contact with the above product.

Support n 877-678-TECH (8324)

info@cellsignal.com

Web n www.cellsignal.com