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PROTEIN STANDARDS Precision Plus Protein WesternC Standards n Protein bands are visible during electrophoresis, after blot

PROTEIN STANDARDS

Precision Plus Protein WesternC Standards

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Protein bands are visible during electrophoresis, after blot transfer, and during chemiluminescent detection

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Sharp bands give accurate molecular weight estimation

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Integral Strep -tag sequence allows chemiluminescent detection

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Dual color makes band reference easy

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Migration pattern is identical to that of other standards in the Precision Plus Protein standards family

Prestained Standards for Chemiluminescent Blot Detection

A precise protein standard is a critical component of every gel electrophoresis experiment. High-quality protein standards should generate sharp, distinct, and reproducible bands for easy and accurate molecular weight determination.

Precision Plus Protein WesternC standards combine the accuracy and convenience of prestained protein markers with integrated chemiluminescent detection to allow visualization during the entire electrophoresis and western blotting process.

Traditional protein standards incorporate natural proteins of predetermined molecular weight for sizing. Bio-Rad’s Precision Plus Protein standards use recombinant proteins for accurate sizing, providing clear and precise bands that are not achievable with native proteins. The family of Precision Plus Protein standards includes WesternC, all blue, Kaleidoscope , dual color, and unstained standards for various experimental applications.

Recombinant Protein Bands

The Precision Plus Protein standards family uses carefully engineered recombinant proteins to generate precise molecular weights. All Precision Plus Protein standards contain ten highly pure recombinant protein bands of 10, 15, 20, 25, 37, 50, 75, 100, 150, and 250 kD.

Chemiluminescent

Membrane Image MW, kD 250 150 100 75 50 37 25 20 15 10
Membrane
Image
MW, kD
250
150
100
75
50
37
25
20
15
10

Precision Plus Protein WesternC standards.

Prestained for Easy Monitoring

The 25, 50, and 75 kD bands in the WesternC

standards are stained pink for easy reference and blot orientation; all other bands are stained blue. These prestained bands are clearly visible during electrophoresis and show equal brightness when transferred onto a membrane. Prestained protein standards make it easy to monitor electrophoresis progress and transfer efficiency.

PROTEIN STANDARDS Precision Plus Protein WesternC Standards n Protein bands are visible during electrophoresis, after blot

Chemiluminescence and Charge-Coupled Device (CCD) Imaging

Precision Plus Protein WesternC standards contain an integrated Strep-tag sequence on each band that enables parallel detection on western blots using a StrepTactin-horseradish peroxidase (HRP) conjugate. Thus, molecular weight of sample proteins can be estimated directly on western blots. The WesternC standards are ideal for visualization of blotting transfer and accurate molecular weight estimation directly from film exposure or CCD images.

Because WesternC standards are part of the Precision Plus Protein standards family, they have clean, sharp bands, enabling generation of publication-quality bands directly on the image.

Ordering Information

Catalog #

Description

161-0376

Precision Plus Protein WesternC Standards,

  • 250 µl, 50 applications

161-0385

Precision Plus Protein WesternC Pack, 50 applications of WesternC standards and 50 applications of StrepTactin-HRP

161-0375

Precision Plus Protein Kaleidoscope Standards,

  • 500 µl, 50 applications

161-0374

Precision Plus Protein Dual Color Standards,

  • 500 µl, 50 applications

161-0373

Precision Plus Protein All Blue Standards,

  • 500 µl, 50 applications

161-0363

Precision Plus Protein Unstained Standards, 1 ml, 100 applications

161-0380

Precision Protein StrepTactin-HRP Conjugate,

  • 300 µl, 150 applications

161-0381

Precision Protein StrepTactin-HRP Conjugate,

  • 125 µl, 50 applications

170-5070

Immun-Star WesternC Chemiluminescent Kit, includes 50 ml luminol/enhancer, 50 ml peroxide solution

170-5044

Immun-Star Goat Anti-Mouse (GAM)-HRP Detection Kit, 500 ml

170-5045

Immun-Star Goat Anti-Rabbit (GAR)-HRP Detection Kit, 500 ml

StrepTactin and Strep-tag are trademarks of Institut für Bioanalytik GmbH.

Strep-tag technology for western blot detection is covered by U.S. patent 5,506,121 and by UK patent 2,272,698. StrepTactin is covered by German patent application P 19641876.3. Bio-Rad Laboratories, Inc. is licensed by Institut für Bioanalytik GmbH to sell these products for research use only.

Bio-Rad Laboratories, Inc.
Bio-Rad
Laboratories, Inc.

Life Science

Group

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US/EG

Rev B

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Sig 1106