RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES

KARNATAKA, BANGALORE.
PROFORMA FOR REGISTRATION OF SUBJECT FOR DISSERTATION
FOR AYURVEDA VACHASPATI (M.D) IN AGADA TANTRA VYAVAHARA
AYURVEDA EVUM VIDHI VAIDYAKA

AN EXPERIMENTAL STUDY TO EVALUATE THE EFFICACY OF

SHATADHOUTA GHRITA APPLICATION IN CONTACT POISONING
CAUSED BY BHALLATHAKA BEEJA. (Semecarpus anacardium Linn)
BY
DR. SRUTHY DAS
Ist year P.G. Scholar
Dept. of P.G Studies in Agada Tantra Vyavahara Ayurveda Evam Vidhi Vaidyaka.
Alvas Ayurveda Medical College & Hospital, Moodbidri. D.K,Karnataka.

Guide
DR. NIRANJANA ACHARYA
Asst. Professor,
Dept. Of PG studies in
Agada Tantra Vyavahar Ayurveda Evum Vidhi Vaidyaka,
Alvas Ayurveda Medical College & Hospital, Moodbidri, D.K, Karnataka.

Co Guide
DR. DEEPTI PATIL
Lecturer,
Dept. Of PG Studies in
Agada Tantra Vyavahar Ayurveda Evum Vidhi Vaidyaka,
Alvas Ayurveda Medical College & Hospital, Moodbidri, D.K, Karnataka.

2012 2013
Alvas Ayurveda Medical College , Moodbidri .

From
Dr. SRUTHY DAS
1st year P.G. Scholar,
Dept. of P.G. Studies in Agada Tantra Vyavahara Ayurveda Evum Vidhi Vaidyaka,
Alvas Ayurveda Medical College & Hospital,
Moodbidri, D.K (Dist) 574227
Karnataka.
To
The Registrar,
Rajiv Gandhi University of Health Sciences,
Bangalore, Karnataka.
Through,
The Principal
Alvas Ayurveda Medical College & Hospital,
Moodbidri, D. K.(Dist) 574227.
Sub: Submission of completed Proforma for registration of subject for Dissertation.
Respected Sir,
I request you to kindly register the synopsis on below mentioned subject in
my name for the submission of dissertation to the Rajiv Gandhi University of health sciences,
Karnataka, Bangalore, for partial fulfillment of M.D (Ayu) Agada Tantra Vyvahara Ayurveda
Evam Vidhi Vaidhyaka.

TITLE OF DISSERTATION:
AN EXPERIMENTAL STUDY TO EVALUATE THE EFFICACY OF
SHATADHOUTA GHRITA APPLICATION IN CONTACT POISONING
CAUSED BY BHALLATHAKA BEEJA .(Semecarpus anacardium Linn)
I am here with enclosing the completed proforma for registration of dissertation subject in
M.D (Ayu) Agada Tantra Vyavahara Ayurveda Evum Vidhi Vaidyaka.
Thanking you,
Yours faithfully,
Place: Moodbidri.
Date:22/04/2013.
(Dr. SRUTHY DAS)

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

KARNATAKA, BANGALORE.
ANNEXURE II
PROFORMA FOR REGISTRATION OF SUBJECT DISSERTATION
1 .NAME AND ADDRESS OF :
THE CANDIDATE

PERMANENT ADDRESS

2. NAME OF THE
INSTITUTION

3. COURSE OF STUDY
AND SUBJECT

Dr. SRUTHY DAS

IST YEAR P.G. SCHOLAR,
DEPT.OF P.G.STUDIES IN AGADA TANTRA
VYAVAHARA AYURVEDA EVUM VIDHI VAIDYAKA,
ALVAS AYURVEDA MEDICAL COLLEGE
&HOSPITAL.
MOODBIDRI, D.K. DISTRICT,
KARNATAKA - 574227.
D/o P.HARIDAS,
KARALASSERYIL HOUSE
PATHIRAPPALLY .P. O,
ALAPPUZHA DIST,
KERALA.
PIN : 688521 .

: ALVAS AYURVEDA MEDICAL COLLEGE

&HOSPITAL.
MOODBIDRI, MANGALORE TALUK,
D.K. DISTRICT,
KARNATAKA - 574227.
: AYURVEDA VACHASPATI (M.D),
AGADA TANTRA VYAVAHARA AYURVEDA
EVUM VIDHI VAIDYAKA.

4. DATE OF ADMISSION
TO THE COURSE

5. TITLE OF THE TOPIC

7/09/2012

AN EXPERIMENTAL STUDY TO EVALUATE THE EFFICACY OF

SHATADHOUTA GHRITA APPLICATION IN CONTACT POISONING
CAUSED BY BHALLATHAKA BEEJA. (Semecarpus anacardium Linn)

6. BRIEF RESUME OF THE INTENDED WORK:

6.1 NEED FOR THE STUDY
Bhallataka (Semecarpus anacardium Linn.,Anacardiaceae) is mentioned under Upavisha1
group in many Ayurvedic classics and it is also described as a poisonous medicinal plant in Drugs
and Cosmetic Act, India (1940)2. Bhallataka has been used for medicinal and non-medicinal
purpose since ancient times. Tarry oil present in the pericarp of the fruit contain Anacardic Acid
that contains urushiols, which cause blisters on contact. So there is every chance of accidental
poisoning causing contact dermatitis in children, and also in adults during the time of Bhallataka
shodhana.
In the treatment of contact poisoning by Bhallataka beeja, application of Shatadhouta
Ghrita as an extreme potent alleviator has been mentioned in Kriya Kaumudi 3. Other references
such as topical application of Shatadhouta Ghrita in treating Visarpa 4(Herpes zoster) is mentioned.
Dalhanacharya has cited that, Goghrita has Vishaghna property5.
Hence an experimental study to evaluate the efficacy of application of Shatadhouta Ghrita,
as a Visha hara dravya in contact dermatitis caused due to Bhallataka beeja has been taken.
6.2 REVIEW OF LITERATURE
Review of literature include a thorough screening of Ayurvedic texts, contemporary
Ayurvedic literatures, modern texts and internet sources to collect sufficient data. Some of the
texts are as follows,
The preparation, ingredients and indication of Shatadhouta Ghrita is available in.

Susruta samhita6 .

Sharangadhara samhita7.

Vaidyaka shabda sindhu8 .

Kriya Kaumudi .

Drug Review:
Sanskrit Name-Bhallataka.
Latin Name-Semicarpus anacardium.Linn.
Family Name-Anacardiaceae.
Kula- Amra kula .
Gana- Deepaneeya gana, Bhedaneeya gana , Kushtaghna Gana, Mutra Sangrahaneeya Gana.
Synonyms- Agnimukhi, Agnika, Arushkara, Viravrksa, Sphotabeejaka, Ranjaka.
Officinal part Fruit, Seed, Oil9.

Previous work done:

1. Singh Omveer A comparative study on the role of nirgundi(vitex nirgundo) and its
root parasite (Alectra Parasita) in the management of occupational skin disorders with
special reference to contact dermatitis.1997, Dept. of Dravya guna.Chitakoot post
graduate institute of Ayurveda, Mahatma Gandhi University Chitrakoot,Satna,MP.
2. Taraphadar

Amit

Dravyaguna(pharmacology)

of

the

extracts

of

Bhallataka(semicarpus Anacardium) and Tikia Koru(Tilicora racemosa) with special

reference to their cytotoxic activities on cultured cells.2000,Dept. of Dravya guna,
Kolkata Institute Of Post Graduate Education and Research in Ayurveda, University
of Kolkata.
6.3 OBJECTIVES OF STUDY

To study the Visa-hara property of Shatadhauta Ghrita in contact poisoning due to

Bhallathaka beeja.

6.4 HYPOTHESIS
H0- There is no effect of Shatadhauta Ghruta in blister`s caused by Bhallathaka beeja.
H1- There is significant effect of Shatadhauta Ghruta as a Visha-hara dravya in blisters caused by
Bhallathaka beeja.

7.

MATERIALS AND METHODS:

7.1 SOURCE OF DATA
A) Literary source:

Major literary source will be collected from Ayurvedic classical textbooks.

Information will be collected from modern texts, internet and relevant research
literature also.

B) Experimental sources:
Semecarpus anacardium Linn :- Seed of this plant is used after authentically
identifying.
Raw drugs required for the preparation of Shatadhouta Ghrita will be collected
from natural source.
Preparation of Shatadhouta Ghrita will be carried out in Alva`s Ayurveda Medical
College.
Animals for the study will be provided from animal house of Alva`s Ayurveda
Research Centre, Moodbidri.
C) Analytical source:

7.2

Analytical study will be carried out in Alva`s Ayurveda Medical College.

1. Organoleptic characters.
2. Physico-chemical analysis.

MATERIALS REQUIRED FOR THE STUDY:

o Seeds of Semecarpus anacardium Linn.
o Shatadhouta Ghrita.
o Facilities for Analytical study.
o Experimental Albino rats (wistar) and Animal house facilities.

METHOD OF COLLECTION OF DATA

1) Pharmaceutical Study:

Shatadhouta Ghrita will be prepared as per Susruta Samhita reference6.

2) Analytical Study:
The sample of Shatadhouta Ghrita will be analyzed with the following tests.
A) Organoleptic Characters
a. Colour
b. Taste
c. Odour
d. Appearance
B) Physico-ChemicalAnalysis
a. pH
b. Refractive index
c. Loss on Drying
d. Specific Gravity
e. Saponification Value
f. Iodine Value
g. Acid Value
h. Rancidity Test

3) Experimental study:
Three groups of six albino rats, not less than eight weeks old weighing about 150200 gms will be taken for experimental study to assess the blister healing property of Shatadhota
Ghrita in contact poisoning caused by Bhallataka beeja, from animal house of Alva`s College of
Ayurveda, Moodbidri.

The blister will be inflicted on rats by external application of Bhallataka beeja niryasa. Totally animals are
divided into 3 groups each group containing 6 animals.
GROUP1- Control
GROUP2- Standard

GROUP3- Test drug

Group

Number

Drug

Form

Purpose

Control

of Rats
Six

No drug

Standard

Six

Silver Nitrate

Ointment

To observe the changes occurring in the

blister and also to compare
with the wound healing process of
other groups.
To assess the healing process.

(Group 2)
Test

Six

Shatadhouta

Ghrita

To assess the healing process.

(Group 1)

application

(Group 3)

Ghrita

Total no of Animals: 18
Route of administration: External application.
Inclusion criteria:
Not less than eight week old adult albino rats of both sexes weighing about 150-200gms will
selected in random.
Exclusion Criteria:
Diseased rats, rats under trial for other experiments and rats below 150gms and more than
200gms.
Interventions:
Duration of experiment: 21days.
Method of application: The Shatadhouta Ghrita is applied over the inflicted wound daily and the
rate of epithelialization is assessed on 7th, 14th and 21st day.
Assessment Criteria:
The fur from the dorsal part of the albino rats will be removed with the help of a razor. The area
has to be marked and apply the sap of the nut shell to the skin of the albino rats externally. Then

wound margins are traced on a trace paper from the day of wounding and inspected for the
percentage of the wound closure on 7th, 14th and 21st day of creating the wound.

Experimental Parameters:
The animals are inspected for the healthy closure of wound and the same is assessed based on the
physical parameters,
1. Wound contraction
2. Duration of healing.

Statistical Plan:
Appropriate test for statistical analysis will be used.

7.3 Does the study require any investigations or interventions to be conducted on Patients or
humans or animals? If so, please describe briefly:
Yes, the study will be conducted on experimental Albino rats (wistar) or animals.

7.4 Has ethical clearance been obtained from your institution in case of 7.3?
Yes, clearance has been obtained from the ethical committee constituted in the institution.

8. LIST OF REFERENCES:
1. Sadananda Sharma. Rasa Tarangini. : Kasinath sastri, editor. 11
Banarasidas; 2004. p.648-9.

th

ed. New Delhi: Motilal

2.Malik V, drugs and cosmetics act 1940 with drug and cosmetic rules, 18th edition , Lucknow
eastern book company, 2005, page 282.
3.VM. Kutti Krishna menon , Kriya kaumudi, National book stall, Kottayam 1986, Sthavara visha
prakaranam. Page No:780, sloka 1095.
4.Kaiyadeva Nighantu, 1st edition, edited by Prof. PV Sharma, Chaukhambha Orientalia ,
Varanasi, 1979 , Page no: 439.
5. Dalhana acharya , Susruta acharya , Gayadasa acharya , edited by Vaidya Jadavji Trikamji
Acharya, Narayan Ram Acharya, Susruta samhita, Published by Chowkhamba Krishnadas
Academy, Varanasi, Kalpasthana, chapter 2 , sloka 42, Page no:566.
6.Susruta acharya , Dalhana acharya, Gayadasa acharya , edited by Vaidya Jadavji Trikamji
Acharya, Narayan Ram Acharya, Susruta samhita,Published by Chowkhamba Krishnadas
Academy, Varanasi, Uttara tantra, chapter 39 , sloka 283, Page no:692.
7.Sharangadhara acharya, Sharangadhara samhita with the commentary of Adhamallas Dipika
and Kasiram,s Gudartha dipika, Chaukamba orientalia, 2008,7th edition, Page no: 361.
8.Kaviraja

umessachandra

Gupta,

Vaidyaka

Sabda

Sindhu,Chaukhamba

orientalia,

Varanasi,UP,1999, 4th edition, Page no:1021.

9.JLN Sastry,Dravya guna Vijnana,Volume 2nd ,Reprint edition:2010,Chaukhamba orientalia
Varanasi ,Chapter23,Page no:135.

9) SIGNATURE OF THE CANDIDATE :

10) REMARKS OF THE GUIDE

11) NAME AND DESIGNATION

11.1) GUIDE
: DR. NIRANJANA ACHARYA
Asst. Professor,
Dept. Of PG studies in Agada Tantra Vyavahar
Ayurveda Evum Vidhi Vaidyaka,
Alvas Ayurveda Medical College,Moodbidri,
D.K, Karnataka.
11.2) SIGNATURE AND SEAL

11.3. CO GUIDE
: DR. DEEPTI PATIL
Lecturer,
Dept. of PG studies in Agada Tantra
Vyavahar Ayurveda Evum Vidhi Vaidyaka,
Alvas Ayurveda Medical college,
Moodbidri,DK,Karnataka
11.4. SIGNATURE AND SEAL

11.5) HEAD OF THE DEPARTMENT

:
DR. NIRANJANA ACHARYA
Asst. Professor,
Dept. Of PG studies in Agada Tantra Vyavahar
Ayurveda Evum Vidhi Vaidyaka,
Alvas Ayurveda Medical College,Moodbidri,
D.K, Karnataka.

11.6) SIGNATURE AND SEAL

12) REMARKS OF THE PRINCIPAL

12.1) SIGNATURE AND SEAL