HEMATOLOGYANALYZER

Service Manual

Tables of Contents
Charpter1 using This Manual.................................2
Charpter2 Hardware..............Error! Bookmark not
defined.
Chapter 3 liquid diagram analysis..........................9
Chapter 4 Histogram and pulse graph..................12
Charpter5 Troubleshouting....Error! Bookmark not
defined.

Charpter1 using This Manual

Introduction
This chapter explains how to use your HEMATOLOGY ANALYZER service manual, which
provides the reference information and procedures needed for servicing your
HEMATOLOGY ANALYZER. Read this manual carefully before servicing your analyzer
and service your analyzer strictly as instructed in this manual.
This manual is to be used in conjunction with the HEMATOLOGY ANALYZER operation
manual and does not contain information and procedures already covered in the
operation manual.

Who Should Read This Manual

This service manual is written for people who
Have a thorough understanding of electronic and fluidic principles.
Have a thorough understanding of reagent systems.
Have a thorough understanding of quality control.
Have a thorough understanding of troubleshooting concepts.
Have an operator's knowledge of the analyzer.
Have the ability to use basic mechanical tools and understand related
terminology.
Have the ability to use a digital voltmeter (DVM) and an oscilloscope.
Have the ability to read electronic and fluidic schematics and understand related
terminology.

Chapter2 Hardware
1. Interface board
(1)the whole block diagram

P104 bus

Motor
control
and drive
unit

CPLD--->printer driver

Oneway

CPLD total
control unit

Valve drive

WBC-RBC enlarge

CPLD<--- WR

CPLD<---HGB

Two-way

Analog power supply

Description:
(1) CPLD total control unit
Control every function module how to communicate with P104
2motor control and driver
Control motor working and amplify signal
3electromagnetic valve driver
3

Printer driver

HGB enlarge

Amplify the signal of electromagnetic valve driver

4printer driver
Amplify the signal of printer driver
5WBC-RBC amplify unit
Amplified WBC-RBC signal that can be more easy to change into digital signal.
6HGB amplify unit
Amplified HGB signal that can be easy to change into digital signal
Supply power to the HGB lamp
7P104 bus
The bus that Connect computer board with CPLD of interface board.
8analog power supply
supply power to each part+48V+12V+8V-8V
protect each part supply power

(2). The Connector Description

printer
J51

J47

J39

6 36 4

2 1

J52

J29

3 2 1

2 1

2 1

P104 Bus
......
...

110V
1 2

J31

WBC-RBC Amplify
sign
J48

CPLD Control

Motor Driver

1 2 3

1 2 3 4

1 2 3

J38

V12

V1

2 1

1 2 3 4
1 2 3 4

1 2 3

3 2 1

WASTE

J45

2 1
J30

4 3 2 1
J50

DLN

CLEAN

V R8

4 3 2 1

Valve Driver Port

Adjustable Resistor

J43

7 6 5 4 3 2 1 J24
SENS D Port

Adjustable Resistor
3 2 1
VR7

JP4

ASP REAGENT

..........
..........
..........
..........
..........
..........
..........
..........
..........
..........
..........

SE N S C Po rt

Mo to r C Po rt

Motor D Port

POWER Control

JP5

2
4
6
8

V R9

J37

Valve Driver

1
3
5
7

Adjustable Resistor

J36

SE N S B Po rt

Mo to r B Po rt

J42

SE N S A Po rt

Mo to r A Po rt

J41

J27

J35

1 2 3

1 2 3

2 1

J40

Adjustable Resistor
3 2 1
VR6
Adjustable Resistor

POWER

LYSE

3 2 1

3 2 1

3 2 1

J32

J33

J34

J49

3 2 1

HGB Amplify
HGB
J44 5 4 3 2 1

VR10

Bexplanation
(1) Motor and sensor input port
(1) J40: motor A motor A in related mechanism fasten board
moduleconnection port
(2) J35: the related sensor A of motor A sensor A in related mechanism fasten
board moduleinput port
(3) J41: motor B motor B in related mechanism fasten board
moduleconnection port
(4) J : the sensor of motor B the sensor B in the mechanism fasten
boardinput port
(5) J42: motor Cmotor C in the mechanism fasten moduleconnection port
(6) J37: the sensor C of motor C the sensor of the related mechanism fasten
boardinput port
4

(7) J43: motor D motor D in the mechanism fasten boardconnection port

(8):J38: the sensor of motor D(the sensor D in the mechanism fasten board
input port

(2) Valve input port

(1) J45:valve input portusing valve from V1 to V11

(3) Waste checking input port

(1) J30: connect to the waste port

(4) Input port of the flow tubing board

(1) J50:the blank line from flow tubing board

(5) Reagent sensor explanation

(1) J32: diluents sensor input port
(2) J33: Rinses sensor input port
(3) J34: Lyses sensor input port

(6) Liquid tubing input port

(1) J24:
(7) power input portJ49

(8) Analog power port

(1) JP5:when you check the situation of interface board, short-circuit port
(2) JP4:the output connector in the analog power part

(9) HGB amplify module

(1) VR6:HGB signal adjustable resistance
(2) VR10point of zero voltage adjustable resistance
(3) J44:related measurement cup port (LED and HGB in)

(10) WBC-RBC amplify module

(1) VR7:PLT amplify adjustable resistance
(2) VR8VR9: adjustable resistance for WBC-RBC-PLT signal
(3) J48:signal input port( WBC-RBC-PLT signal input line)
(4) J31:110V voltage input port
(11) APS indicated lamp portJ29
(12) The drive line of black light portJ39
(13) Port of printer drive boardJ47

(14) CPLD control module

(1) J27: burn software for 95288
(15) PC104 bus
(1) J51J52: compose with two part

2. Control board
(1). The Connector Description

()corresponding port introduction

(1) CN15: connect to display
(2) CN14: connect to CF card
(3) CN12: power of control board
(4) CN8CN9connect to interface board
(5) CN6: mouse and keyboard port
(6) CN7: connect external parallel port printer from 19 pin
(7) CN1: connect buzzer
(8) CN17: connect to display
(9) CN2: connect serial line (ex: printer)
(10) CN11: connect USB mouse and keyboard

Chapter 3 liquid diagram analysis

According the liquid diagram, there are several kinds of functions.
1. diluent analysis

2. rinse analysis

3. lyse analysis
7

4. test process analysis

5. Waste Analysis

6. enhanced cleaning

Chapter 4 Histogram and pulse graph

1. HISTOGRAM
There are some practical WBC histograms.
(1). Normal WBC histogram

Description: the space between the first line and the second line is lymphocyte; the
space between the second line and the third line is intermediate cell; the space between the
third line and the fourth line is granulocyte.

(2). Histogram compress too narrow without classification

(3). WBC<0.5, without classification

(4).peak value in the middle, without classification

(5). Nucleated RBC increase or interference or hemolysis insufficiency

10

(6) WBC serious interference

(7). without lyse or Hemolytic effect is not good

(8). Increased neutrophils

(9). Increased lymphocytes

(10). Patients with tumor

11

(11). Increased monocyte

2. Pulse graph
After test, the pulse graph will be saved in the memory, it
can help you to analyze the problem.
(1) Normal Pulse Graph
Select special, then input the password: 6666, press F5 to
check WBC pulse graph; press F6 to check RBC pulse graph; press
F7to check PLT pulse graph; press F1 to return to main menu.
* The normal range of total pulse for normal people:
WBC< 1M
RBC< 600K
PLT< 1M
* the normal range of total pulse for normal QC:
WBC400 700K
RBC250450K
PLT300 600K
Below the normal pulse graph.

WBC pulse graph of normal sample

WBC pulse graph of blank

RBC pulse graph of normal sample

RBC pulse graph of blank

12

PLT pulse graph of normal sample

PLT pulse graph of blank

3. abnormal pulse graph

WBC serious interference (Blank)

WBC serious interference (normal sample)

RBC serious interference (Blank)

RBC serious interference (normal sample)

PLT serious interference (Blank)

sample)

13

PLT serious interference (normal

PLT high-gain (Blank)

PLT high-gain (normal sample)

WBC Slight disturbance (normal sample) not enough lyse or without

hemolysis

RBC Slight disturbance (normal sample) high concentration sample for

14

RBC

PLT Slight disturbance (normal sample) high concentration sample for PLT

Charpter5 Troubleshouting
Problem
description
WBCRBC
bubble

15

reason

Solution

When liquid flow into the

metering uint
A if there are some bubble in
the liquid,
B if the liquid flow too fast
C if liquid flow into flow
sensor , the two LED is
always on or the down LED
is always on

A: do the function: cleaning

B: 1 according the liquid diagram
to check the tubing
whether is
there leakage
2enter into submenu
:mechanicpassword:6800and
check each solenoid valve .
C: change the metering unit

WBC and
RBC clog

When liquid flow into the

metering uint
A the liquid stop flowing
B the liquid flow along wall
C the liquid flow is normal
D the liquid flow slowly
E the second foot of interface
board
connector J50 is
disconnected with ground

A The most possible is block

1.enhanced flush 2 cauterant the
aperture
(enter
mechanic:
password is 6800,) 3 clean the
aperture and reassemble the
chamber, 4. according the liquid
diagram, check the block
B use EZ cleaner to clean the
metering unit ( put the rinse tube
into EZ cleaner, then do the
function: enhanced cleaning
C check the two LEDs of metering
unit, if the LED is always off,
change the the board
D 1 enhances flush 2 cauterant
the aperture (enter mechanic:
password is 6800,) 3 clean the
aperture and reassemble the
chamber 4. according the liquid
diagram, check the block
E connect the second foot to the
ground

WBC or RBC
clog

When liquid flow into the

metering uint
A the liquid flow along wall
B the liquid flow is normal C
the liquid flow slowly

A use EZ cleaner to clean the

metering unit ( put the rinse tube
into EZ cleaner, then do the
function: enhanced cleaning
B check the two LEDs of metering
unit, if the LED is always off,
change the the board
C 1 enhances flush 2 cauterant
the aperture (enter mechanic:
password is 6800,) 3 clean the
aperture and reassemble the
chamber 4. according the liquid
diagram, check the block

5.enter into special(password:

6868), increase the setting
time
No measuring
time

16

When liquid flow into the

metering uint
A
the upside LED of
metering unit is always off
B the the liquid stop flowing

A change the metering unit

B the most possible is block

1.enhanced flush 2 cauterant the

aperture
(enter
mechanic:
password is 6800,) 3 clean the
aperture and reassemble the
chamber, 4. according the liquid

diagram, check the block

HGB
failure HGB
bubble

Hemoglobin
unstable

17

enter into mechanic

(password:6800) and check
the HGB blank voltage;
the normal range 4.254.31
A if the voltage is 0V, 1.the
HBG lamp is off 2. the HGB
photometer is failure
B 0< HGB voltage <= 5

A 1. Check the 1 pin of U57, if the

voltage is 5V change the HGB
lamp; if not, change the interface
board 2. reconnecting the receiver
line
B adjust the resistance VR6 to
normal range 4.254.31V

A other
unstable,
B other
stable

A check the sampling pump, there

is leakage , change the gasket ring
B
enter
into
mechanic(password:6800) check
the HGB blank voltage fluctuation ,
if the HGB blank voltage is larger
than 0.2V, change the HGB lamp

parameters

are

parameters

are

PLT value is
too high in
ground test

A ground wire problem

B it is shared socket with
large efficient machine
C
whether
environment
temperature is suit for the
request of the machine
D the route maintenance
:once every week
E whether the reagent is
expired date or sordid
F check every part especially
chamber,1. it is rusting2. the
shielding box connect to
ground.(how to check it:
disconnect the signal wire,
check the connection of
shielding box and ground

A check the voltage of the

groundwire and zero curve
If it is less than 5V, it is OK,
if it is more than 5V , reconnect
ground wire
B use a separate socket
C environment temperature is suit
for the request of the machine
D if not, use the function park
clean all the tubing
E change the reagent
F change the shielding shim.

PLT unstable

A ground wire problem

B it is shared socket with
large efficient machine
C
whether
environment
temperature is suit for the
request of the machine
D the route maintenance
:once every week

A check the voltage of the ground

wire and zero curve
If it is less than 5V, it is OK,
if it is more than 5V , reconnect
ground wire
B use a separate socket
C environment temperature is suit
for the request of the machine

E whether the reagent is

expired date or sordid
F check every part especially
chamber,1. it is rusting2.
The shielding box connect to
ground.(how to check it:
disconnect the signal wire,
check the connection of
shielding box and ground

D if not, use the function park

clean all the tubing
E change the reagent
F changes the shielding shim.

All the items

unstable

A ground wire problem

B it is shared socket with
large efficient machine
C
whether
environment
temperature is suit for the
request of the machine
D the route maintenance
:once every week
E whether the reagent is
expired date or sordid
F check every part especially
chamber,1. it is rusting2. the
shielding box connect to
ground.(how to check it:
disconnect the signal wire,
check the connection of
shielding box and ground
G there is leakage in
sampling pump

A check the voltage of the ground

wire and zero curve
If it is less than 5V, it is OK,
if it is more than 5V , reconnect
ground wire
B use a separate socket
C environment temperature is suit
for the request of the machine
D if not, use the function park
clean all the tubing
E change the reagent
F changes the shielding shim.
G check the sampling pump, and
change the gasket ring

Vacuum pump
skid and make
a big noise

the vaccum pump move

up or down continuously
the vaccumpump skid
when the vaccum pump
stop moving, the piston come
out from vacuum chamber.

check whether the voltage in

the first foot of connector J36 is

1.2V.if not, change the sensor.
Check the voltage of three foot in
connector, when the sheet metal
enters into sensor, the voltage is
4.0-5.0V; when the sheet metal is
out of sensor, the voltage is 0.01.0V. if not ,change it
B changes the rack and the gear.
C the waste tubing flow disfluency,
check the block place and clean it.

The value of A if the value of HGB is zero, A prime lyse, or change the piston
WBC is * or the lyse is not enough in the gasket ring of sampling room,
tube.
B if the total pulse of WBC less
0
B If the value of HGB is not
zero, after test QC or sample
18

than 5000, change the interface

board

RBC no value

check the pulse of

RBC,RBC,PLT(enter into
special, the password is
6666
(press F5 to check the pulse
of WBC)
C enter into mechanic
(password: 6800), check the
function sampling break

If the total pulse of WBC is too

high, adjust the potentiometer VR8
clockwise
If the total pulse of WBC is too
high , adjust the potentiometer
VR9 anticlockwise
C if the function is ok, try to
reconnect CONTROL BOARD, if it
is failed , change the interface
board

A after test QC or sample

check the pulse of RBC,
(enter into special, the
password is 6666
(press F6 to check the pulse
of RBC)
B enter into mechanic
(password: 6800), check the
function sampling break

A if the total pulse of WBC less

than 5000, change the interface
board
If the total pulse of WBC is too
high, adjust the potentiometer VR8
clockwise
If the total pulse of WBC is too
high, adjust the potentiometer VR8
anticlockwise.
B if the function is ok, try to
reconnect CONTROL BOARD, if it
is failed , change the interface
board

PLT no value

A enter into mechanic

(password: 6800), check the
function sampling break
B after test QC or sample
check the pulse of RBC,
(enter into special, the
password is 6666
(press F7 to check the pulse
of PLT)

A if the function is ok, try to

reconnect CONTROL BOARD, if it
is failed , change the interface
board
B if the total pulse of PLT less than
5000, change the interface board
If the total pulse of WBC is too
high, adjust the potentiometer VR8
clock wisely
If the total pulse of WBC is too
high, adjust the potentiometer VR7
anticlockwise.

distort no lyse

A check the voltage of 1th

pin of J34 it is about 1.2V
normally
B check the voltage of 3th
pin it is more than 3.0V
when the tube is filled with

A if not, the sensor have broken,

change it
B if the voltage is about 5V
or0V, change the sensor. If the
voltage is between 2.0V and 3.0V,
try to clean the white reagent

19

distort no
diluent

reagent; it is less than 2.0V

when the tube is empty

detector.

A check the voltage of 1th

pin of J32 it is about 1.2V
normally
B check the voltage of 3th
pin it is more than 3.0V
when the tube is filled with
reagent; it is less than 2.0V
when the tube is empty

A if not, the sensor have broken,

change it
B if the voltage is about 5V
or0V, change the sensor. If the
voltage is between 2.0V and 3.0V,
try to clean the white reagent
detector.

distort no rinse A check the voltage of 1th A if not, the sensor have broken,
pin of J33 it is about 1.2V
normally
B check the voltage of 3th
pin it is more than 3.0V
when the tube is filled with
reagent; it is less than 2.0V
when the tube is empty

change it
B if the voltage
or0V, change the
voltage is between
try to clean the
detector.

not be good at
classification

A inappropriate magnification
B inappropriate sampling
C inappropriate store of
blood

A use the quality control to adjust

the diagram of WBC
B check the sampling
C check the expiry date, test
another new blood

Only blank in
the screen

A no display but other function

A check the connector of screen

B reconnect the control board or
change the CF card or change the
control board

is OK
B no display and no other
function

Full waste
alarm

alarm if disconnect the

waste sensor
B it is OK when it is not use
the waste sensor

20

is about 5V
sensor. If the
2.0V and 3.0V,
white reagent

A try to change the interface baord

B clean the waste sensor or
change it