Description

ProductSheet

RPMI8226(ATCCCCL
155)
PleasereadthisFIRST

StorageTemp.
liquidnitrogen
vaporphase

BiosafetyLevel
1

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CompleteGrowthMedium
ThebasemediumforthiscelllineisATCCformulated
RPMI1640Medium,CatalogNo.302001.Tomakethe
completegrowthmedium,addthefollowing
componentstothebasemedium:fetalbovineserumto
afinalconcentrationof10%.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:RPMI8226(ATCCCCL155)

Organism:Homosapiens,human
Isotype:lambdalightchain
Tissue:peripheralblood
Disease:plasmacytomamyeloma
CellType:Blymphocyte
Age:61years
Gender:male
Morphology:lymphoblast
GrowthProperties:suspension
Isoenzymes:
G6PD,A
DNAProfile:
Amelogenin:X,Y
CSF1PO:12
D13S317:11
D16S539:9
D5S818:11,13
D7S820:9,10
THO1:8
TPOX:8,11
vWA:16,18
CytogeneticAnalysis:Unstablekaryotypeintriploidrangeof6870chromosomes.Twolargemarker
chromosomeswithterminalcentromeres.

BatchSpecificInformation
RefertotheCertificateofAnalysisforbatchspecifictestresults.

SAFETYPRECAUTION
ATCChighlyrecommendsthatprotectiveglovesandclothingalwaysbeusedandafullfacemaskalwaysbe
wornwhenhandlingfrozenvials.Itisimportanttonotethatsomevialsleakwhensubmersedinliquidnitrogen
andwillslowlyfillwithliquidnitrogen.Uponthawing,theconversionoftheliquidnitrogenbacktoitsgas
phasemayresultinthevesselexplodingorblowingoffitscapwithdangerousforcecreatingflyingdebris.

Unpacking&StorageInstructions
1. Checkallcontainersforleakageorbreakage.
2. Removethefrozencellsfromthedryicepackagingandimmediatelyplacethecellsatatemperature
below130C,preferablyinliquidnitrogenvapor,untilreadyforuse.

HandlingProcedureforFrozenCells

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page1of3

Toinsurethehighestlevelofviability,thawthevialandinitiatethecultureassoonaspossibleuponreceipt.If
uponarrival,continuedstorageofthefrozencultureisnecessary,itshouldbestoredinliquidnitrogenvapor
phaseandnotat70C.Storageat70Cwillresultinlossofviability.
1. Thawthevialbygentleagitationina37Cwaterbath.Toreducethepossibilityofcontamination,keep
theOringandcapoutofthewater.Thawingshouldberapid(approximately2minutes).
2. Removethevialfromthewaterbathassoonasthecontentsarethawed,anddecontaminateby
dippinginorsprayingwith70%ethanol.Alloftheoperationsfromthispointonshouldbecarriedout
understrictasepticconditions.
3. Transferthevialcontentstoacentrifugetubecontaining9.0mLcompleteculturemediumandspinat
approximately125xgfor5to10minutes.
4. Resuspendthecellpelletwiththerecommendedcompletemedium(seethespecificbatchinformation
fortheculturerecommendeddilutionratio)anddispenseintoa25cm2ora75cm2cultureflask.Itis
importanttoavoidexcessivealkalinityofthemediumduringrecoveryofthecells.Itissuggestedthat,
priortotheadditionofthevialcontents,theculturevesselcontainingthecompletegrowthmediumbe
placedintotheincubatorforatleast15minutestoallowthemediumtoreachitsnormalpH(7.0to7.6).
5. Incubatethecultureat37Cinasuitableincubator.A5%CO2inairatmosphereisrecommendedif
usingthemediumdescribedonthisproduct.

HandlingProcedureforFlaskCultures

ProductSheet

RPMI8226(ATCCCCL
155)
PleasereadthisFIRST

StorageTemp.
liquidnitrogen
vaporphase

Theflaskwasseededwithcells(seespecificbatchinformation),grown,andcompletelyfilledwithmediumat
ATCCtopreventlossofcellsduringshipping.
1. Uponreceiptvisuallyexaminethecultureformacroscopicevidenceofanymicrobialcontamination.
Usinganinvertedmicroscope(preferablyequippedwithphasecontrastoptics),carefullycheckfor
anyevidenceofmicrobialcontamination
2. Incubatetheflaskinanuprightpositionforseveralhoursat37C.Afterthetemperaturehas
equilibrated,asepticallyremovetheentirecontentsoftheflaskandcentrifugeat125xgfor5to10
minutes.Removeshippingmediumandsaveforreuse.Resuspendthecellpelletin10mLofthis
medium.
3. Fromthiscellsuspensionremoveasampleforacellcountandviability.Adjustthecelldensityofthe
suspensionto5x105viablecells/mLintheshippingmedium.
4. Incubatetheculture,horizontally,at37Cina5%CO2inairatmosphere.Maintainthecelldensityof
thecultureassuggestedunderthesubcultureprocedure.

SubculturingProcedure
Culturescanbemaintainedbytheadditionoffreshmediumorreplacementofmedium.Alternatively,cultures

BiosafetyLevel
1

canbeestablishedbycentrifugationwithsubsequentresuspensionat5x105viablecells/mL.Donotallow
thecelldensitytoexceed2to3x106cells/mL.CorningT75flasks(catalog#431464)arerecommendedfor
subculturingthisproduct.
Interval:Maintaincelldensitybetween5x105and2x106viablecells/mL.
MediumRenewal:Every2to3days

IntendedUse

CryopreservationMedium
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CompleteGrowthMedium
ThebasemediumforthiscelllineisATCCformulated
RPMI1640Medium,CatalogNo.302001.Tomakethe
completegrowthmedium,addthefollowing
componentstothebasemedium:fetalbovineserumto
afinalconcentrationof10%.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:RPMI8226(ATCCCCL155)

Completeculturemediumdescribedabovesupplementedwith5%(v/v)DMSO.
CellculturetestedDMSOisavailableasATCCCatalogNo.4X.

Comments
Thereisnoevidenceofheavychainproduction(cytoplasmicorsecreted).

References
Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.

BiosafetyLevel:1
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

ATCCWarranty
TheviabilityofATCCproductsiswarrantedfor30daysfromthedateofshipment,andisvalidonlyifthe
productisstoredandculturedaccordingtotheinformationincludedonthisproductinformationsheet.ATCC
liststhemediaformulationthathasbeenfoundtobeeffectiveforthisstrain.Whileother,unspecifiedmedia
mayalsoproducesatisfactoryresults,achangeinmediaortheabsenceofanadditivefromtheATCC
recommendedmediamayaffectrecovery,growthand/orfunctionofthisstrain.Ifanalternativemedium
formulationisused,theATCCwarrantyforviabilityisnolongervalid.

Disclaimers
AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page2of3

Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
confirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
ismadetoinsureauthenticityandreliabilityofstrainsondeposit,ATCCisnotliablefordamagesarisingfrom

themisidentificationormisrepresentationofcultures.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

ProductSheet

RPMI8226(ATCCCCL
155)
PleasereadthisFIRST

StorageTemp.
liquidnitrogen
vaporphase

BiosafetyLevel
1

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CompleteGrowthMedium
ThebasemediumforthiscelllineisATCCformulated
RPMI1640Medium,CatalogNo.302001.Tomakethe
completegrowthmedium,addthefollowing
componentstothebasemedium:fetalbovineserumto
afinalconcentrationof10%.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:RPMI8226(ATCCCCL155)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page3of3

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2014.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[08/25]