Beruflich Dokumente
Kultur Dokumente
AND
ITS APPENDAGES
Histology lecture by
Doc. MUDr.
MUDr. Kraj
Kraj
Dimitrolos
Dimitrolos,, CSc
Department of
Histology & Embryology
Faculty of Medicine
Palacky University, Olomouc, CZ
10/2009
CHAPTER OUTLINE
A]
B]
C]
D]
E]
THE EPIDERMIS
THE DERMIS
GLANDS OF THE SKIN
THE HAIR
THE NAIL
[w]
SKIN - introduction
The heaviest single organ of the body (16% of the total body wt.)
It covers 1.2 - 2.3 m2 surface exposed to the external environment
----------------FUNCTIONS of the skin:
a) Protection against the external ENVIRONMENT.
b) Prevention of extreme WATER LOSSES by evaporation.
c) Protection against MECHANICAL INJURY (friction
(friction).
).
d) THERMOPROTECTION , UV - rays protection.
e) RECEPTOR ORGAN.
f) EXCRETION of various substances, metabolites.
g) Formation of VITAMIN D3.
[?]
[W]
EPIDERMIS
This layer consists of the STRATIFIED
SQUAMOUS KERATINIZED EPITHELIUM
EPIDERMIS
Horny cells
Langerhans
cell
Flattened keratinocyte
Keratinocyte
Melanin
granules
Spinous keratinocyte
Melanocyte
Dermis
Basal keratinocyte
EPIDERMIS
STRATUM CORNEUM
[8]
EPIDERMIS
This layer consists of the STRATIFIED
SQUAMOUS KERATINIZED EPITHELIUM
[10]
EPIDERMIS
[1] STRATUM BASALE :
(GERMINATIVUM)
Features :
Basophilic columnar cells adhere
to basal lamina.
Hemidesmosomes at basal lamina
(dermal <
<> epidermal junction).
Some cytokeratin filaments.
Frequent mitoses - cell turnover
is approx. 15 - 30 days.
BASAL KERATINOCYTE
[10]
EPIDERMIS
[1] STRATUM BASALE :
(GERMINATIVUM)
Features :
Basophilic columnar cells adhere
to basal lamina.
Hemidesmosomes at basal lamina
(dermal <
<> epidermal junction).
Some cytokeratin filaments.
Frequent mitoses - cell turnover
is approx. 15 - 30 days.
[3]
EPIDERMIS
BASAL KERATINOCYTES
[8]
EPIDERMIS
[2] STRATUM SPINOSUM :
Features::
Features
Cuboidal (polyhedral) cells.
Tonofibrils are abundant in their
cytoplasm.
Numerous desmosomes join the
cells together (giving these cells
spiny appearance).
SPINOUS KERATINOCYTE
[10]
EPIDERMIS
[2] STRATUM SPINOSUM :
Features::
Features
Cuboidal (polyhedral) cells.
Tonofibrils are abundant in their
cytoplasm.
Numerous desmosomes join the
cells together (giving these cells
spiny appearance).
SPINOUS KERATINOCYTE
[7]
EPIDERMIS
[2] STRATUM SPINOSUM :
Features::
Features
Cuboidal (polyhedral) cells.
Tonofibrils are abundant in their
cytoplasm.
Numerous desmosomes join the
cells together (giving these cells
spiny appearance).
SPINOUS KERATINOCYTE
[7]
N2
N1
[dk]
N2
N1
[dk]
[7]
EPIDERMIS
[3] STRATUM GRANULOSUM :
Features::
Features
GRANULAR KERATINOCYTE
EPIDERMIS
[3] STRATUM GRANULOSUM :
Features::
Features
GRANULAR KERATINOCYTE
[8]
EPIDERMIS
[3] STRATUM GRANULOSUM :
Features::
Features
EPIDERMIS
[4] STRATUM LUCIDUM :
Features::
Features
Flattened pale eosinophilic cell.
No organelles and nuclei.
EPIDERMIS
[4] STRATUM LUCIDUM :
SCor
Features::
Features
Flattened pale eosinophilic cell.
No organelles and nuclei.
SSp
EPIDERMIS
[5] STRATUM CORNEUM :
HORNY CELLS
Features:
Features:
15 - 20 layers of flattened
non--nucleated and fully
non
keratinized cells.
EPIDERMIS
[5] STRATUM CORNEUM :
HORNY CELLS
Features:
Features:
15 - 20 layers of flattened
non--nucleated and fully
non
keratinized cells.
[8]
Keratinocytes in strat.
corneum, EM x 6000
[1]
EXPRESSION
OF KERATINS
IN ASCENDENT
LAYERS OF
THE EPIDERMIS
[?]
keratinocytes
2]
1] MELANOCYTES
2] LANGERHANS
CELLS
Langerhans
cell
1]
3]
3] MERKELS
CELLS
[1]
1]
Melanocytes
Melanocytes
[1]
1]
MELANOCYTES
Are specialized cells
of the epidermis
producing pigments
EUMELANIN
(brown pigment) or
PHEOMELANIN
(red--brown pigment).
(red
Cell bodies are found
between the cells of
stratum basale.
[7]
MELANOCYTES
EM:
[1]
2]
LANGERHANS CELLS
LANGERHANS CELL
[dk]
BIRBECKS GRANULES
EM x 18 000
Function:: - These cells are responsible for processing and presenting of
Function
antigens within the epithelium to lymphoid cells
- they arise from the bone marrow precursor cells (mononuclear
phagocyte system)
[dk]
FUNCTION OF
LANGERHANS CELLS
[1]
3]
MERKELS CELLS
Hairless skin
Sensory transduction.
[1]
[10]
STRUCTURE OF DERMIS
It consists of :
CONNECTIVE TISSUE with
GLANDS, HAIR FOLLICLES,
BLOOD VESSELS, and
SENSORY NERVE
ENDINGS.
LAYERS OF THE DERMIS:
1] PAPILLARY LAYER :
(the outermost) forms
DERMAL PAPILLAE
2] RETICULAR LAYER : (The inner thicker layer)
Papillary
layer
Reticular
layer
[W]
Papillary layer
+ BASAL LAMINA
+ RETICULAR LAMINA
+ ANCHORING FIBRILS.
[8]
[8]
[4]
[8]
[2]
Reticular layer
[8]
[7]
[A] ECCRINE
SWEAT GLANDS
[B] APOCRINE
SWEAT GLANDS
[C] SEBACEOUS
GLANDS
[w]
2)
[7]
[7]
[1]
[2]
2) DUCT PORTION :
Two
Two--layered cuboidal epithelial cells
of dense appearance form a straight
tubulus transversing the papillary
layer of the dermis.
Lumen
Lumen--bordering cells have
prominent (eosinophilic) terminal web
(reabsorption of Na).
There is no duct within the epidermis,
the sweat penetrates through a system
of spiralspiral-shaped channels called
ACROSYRINGIUM and opens up
on the surface the stratum corneum.
[7]
[13]
[1]
[6]
[1]
Basal cells
LM x 450
[7]
Sweat gland
[8]
Sebaceous gland
[8]
[?]
[3]
[13]
ypodermis
[6]
[6]
[6]
[8]
HAIR BULB
DERMAL PAPILLA
[6]
[?]
HAIR BULB
[7]
[?]
HAIR BULB
DERMAL PAPILLA
[6]
[?]
[8]
[7]
[?]
[13]
[6]
H
APM
[8]
[1]
[1]
[1]
[1]
Fingernail, l.s., LM x 14
[8]
[1]
Meissners corpuscle
corpuscle,, LM x 540
[8]
[8]
MAMMARY GLAND
It is a compound
tubulo-alveolar gland
consisting of 15 - 25
lobes located in a
connective and
adipose tissues of the breast.
[10]
[8]
12 w.
25 w.
30 w.
[17]
[17]
[10]
[8]
E] POSTPOST-LACTATIONAL REGRESSION
With cessation of breast-feeding
most alveoli undergo
desquamation and sloughing of
whole cells + autophagic
absorption of cell organelles with
help of macrophages.
Myoepithelial and basal cells
persist as cords of cells.
F] SENILE INVOLUTION
After menopause the involution
encounters a reduction in size
and atrophy of secretory portions
of some ducts and glands
+ reduction of the interlobular
connective tissue.
Development of DERMIS :
It is derived from dermatomes of somites
and lateral plate mesoderm (mesenchyme).
It forms the superficial corium (dermal papillae)
and subcorium (the deeper layers
of the dermis).
At birth the skin is covered by vernix caseosa,
the product of
sebaceous
glands and
degenerated
OF SWEAT
epidermal cells. DEVELOPMENT
GLANDS
[17]
[17]
DEVELOPMENT OF NAILS
At 10 weeks - primordia in epidermis - NAIL FIELDS develop at the tips
of fingers and toes.
Lateral and proximal NAIL FOLDS surround the nil field.
The cells of he proximal nail fold grow over the nail field, become
keratnized and form the NAIL PLATE.
PLATE
The development of fingernails precedes that of toenails by 4 weeks.
10 weeks
32 weeks
[17]
The End
[dk]
REFERENCES
10
REFERENCES
11
12
13
16
17
18
14
15
FULL REFERENCES
(1) Kierszenbaum A.L.: Histology and Cell biology, Mosby, London, 2002
(2) Fawcett D.W., Jensh R.P.: Concise Histology, Chapman&Hill, New York, 1997
(3) Stevens A., Lowe J.: Human Histology, 2nd ed., Mosby, London, 2000
(4) Cormack D.H.: Essential Histology, Lippincott-Raven, Philadelphia, 1997
(5) Cohen B.D., Wood D.L.: Structure and Function of the Human Body,
7th ed. Lippincott Williams & Wilkins, Philadelphia, 2000
(6) Kerr J.B.: Atlas of Functional Histology, Mosby, London, 2000
(7) Junqueira L.C., Carneiro J., Kelly R.O.: Basic Histology, Text and Atlas
[?]
FULL REFERENCES
10) Ross M.H., Kaye G.I., Pawlina W.:Histology, text and atlas, 4th ed., Lippincott
Williams & Wilkins, Philadelphia, 2003
11) Krstic R.V.: Human Microscopic Anatomy, Atlas for students of Medicine and
Biology, Springer-Verlag, Berlin, 1991
12) Ross M.H., Reith E.J.: Histology, Text and Atlas, Harper& Row, Lippincott
Co., New York, 1985
13) Roroschenko V.P.:Di Fiores Altlas of Histology with Functional correlations,
9th ed., Lippincott Williams & Wilkins, Philadelphia, 2000
14) Lamberti A., MarinoT., Sodicoff M: The Histology lab assistant. Interactive
CD-ROM , AGC Educational Media,
15) Sadler T.W.: Langmans Medical Embryology, 8th ed., Lippincott Williams &
Wilkins, Philadelphia, 2000
16) Gartner L.P., Hiatt J.L.: Color Textbook of Histology, 2nded., W.B. Saunders
Co., Philadelphia, 2001
17) Moore KL, Persaud TVN: The Developing Human. Clinically Oriented
Embryology, 8th ed., Saunders, Elsevier Philadelphia, 2008.
18) Carlson BM: Human Embryology and Developmental Biology, 3rd ed., Mosby,
Reference numbers are added to pictures in their right bottom corner.
2004
[?]
INTERNET RESOURCES
http://www.visualhistology.com/products/atlas/VHA_Chpt9_Skin.html
https://histo.life.illinois.edu/histo/atlas/showcat.php?cat_to_show=N&w=500
http://www.youtube.com/watch?v=-KmfEbKmKFw&feature=related
http://www.youtube.com/watch?v=ghWq2zjtE2w&feature=channel
http://www.ihcworld.com/video/histology/integument.htm
http://www.search.eb.com/eb/art-18506/Animation-and-microphotography-showing-the-skins-three-layers-theepidermis
http://www.anatomyatlases.org/MicroscopicAnatomy/Section07/Section07.shtml
http://www.path.uiowa.edu/cgi-bin-pub/vs/vl_browse.cgi?cat=h_integument
http://projects.galter.northwestern.edu/rhodin/
http://www.siumed.edu/~dking2/intro/skin.htm
http://www.meddean.luc.edu/lumen/MEdEd/Medicine/dermatology/melton/skinlsn/sknlsn.htm
http://www.histology-world.com/photoalbum/thumbnails.php?album=23