Beruflich Dokumente
Kultur Dokumente
Upright Microscope
System expandability
Optical performance
CFI Plan Apochromat objectives with chromatic aberration correction and high
transmission throughout a broad range of wavelengths
Objectives with improved transmission in near IR wavelength range for multiphoton
excitation imaging
Design
Operability
Ni-E: Motorized model with automatic changeover of observation methods and
adjustment of microscope accessories.
Ni-U: Manual model with some motorized options.
Most microscope controls can be operated with easy-to-reach buttons on the front of
the Ni-E.
Genetics
Immunology
Cell biology
Microbiology
Pathology
Neuroscience
Drug discovery
Cancer research
Developmental
biology
Demand for multi-color fluorescent imaging that uses newly developed fluorescent proteins and fluorescent reagents is
constantly increasing. Nikon meets such needs with diverse functions and optical technologies.
Research into the reactions and changes of stimulated cells has become popular in recent years. Nikon has developed a
new photoactivation unit for upright microscopes, a first in microscopy.
Total Power
500mW MAX.
CW 400700nm
IEC/EN60825-1:2007
Complies with FDA performance standard
for laser products except for
deviations pursuant to Laser Notice
No.50,dated June 24,2007.
The higher speeds of the motorized excitation and barrier filter wheel and the motorized shutter enable
quick wavelength changes, reducing photobleaching of the specimen.
It can be operated via easily accessible control buttons, increasing operation efficiency.
Ni-E
Multi-color
fluorescent
observation
With CFI Plan Apochromat objectives, chromatic aberration has been corrected up to 850 nm and
transmission improved in the long wavelength range, increasing accuracy and efficiency of laser excitation
at target site.
FISH
Photo
activation
FRAP
Signal
conversion
Ni-U
Ni-E
This structure allows two-layer mounting of a photoactivation unit with an epi-fluorescence cube turret.
Ni series accessories are segmented by function, allowing you to select required units and
flexibly combine them to create lean and effective system configurations.
Ni-E
Optimal brightness can be automatically adjusted with objective changeover, eliminating the need for manual adjustment.
As operations for optical zooming can be controlled from the control unit DS-L3, images of the desired angle of field can
be captured while maintaining image quality.
Two-photon
imaging
Configuration of motorized microscope Ni-E with
motorized quadrocular tilting tube, motorized
DSC zooming port, motorized ND filter, digital
camera DS-Fi2, motorized XY stage and joystick,
control unit DS-L3, control box A
Photo
activation
To meet diverse user demands for operability, system expandability and motorized control, Nikon provides two Ni series
models. The Ni-U, which has compatibility with some motorized accessories, is the manual model suitable for high-quality
image observation and digital imaging. The Ni-E is a fully motorized model that is efficient for experiments requiring
comprehensive control of various devices, such as photoactivation units and confocal systems.
Condenser, aperture and field diaphragm, and ND filter are automatically set to the optimal
position during objective changeover. In addition, stage XYZ travel amount per handle
rotation and parfocal distance deviation correction are automatically adjusted.
Microscope settings can also be manually adjusted.
10
11
Nikons proprietary noise terminator mechanism is employed in the epifluorescence cube turret and filter cubes. The S/N ratio has been dramatically
improved by thoroughly eliminating stray light in the filter cubes, allowing
images of weak fluorescent signals to be captured with high contrast and
brightness.
With remarkably high NA, greatly improved transmission in the long wavelength
range thanks to Nikon proprietary Nano Crystal Coat, and chromatic aberration
correction over 435 to 850 nm, these objectives are ideally suited not only for
brightfield and DIC observations but also for fluorescent observations. These lenses
allow acquisition of bright and clear images at any wavelength for near-IR imaging
and multi-color fluorescence imaging. Because bright images can be captured even
with a weak excitation light, damage to a specimen is minimized.
Camera
Fluorescence
filter cube
ND
Light
source
Stray light
Incident light
Reflected light
Incident light
Reflected light
Light absorbing
material
Lens
Lens
Lens
12
Epi-fluorescence observation
Brightfield observation
13
The Ni series can be controlled in conjunction with a Digital Sight series digital camera, facilitating
effortless digital imaging. Images can be captured with a dedicated button on the microscope body,
the touch panel on the camera control unit, or the software GUI on a PC, depending on your preference.
A wide array of models with high sensitivity, high resolution, high speeds and faithful
color reproduction are available to meet all research needs and purposes.
Microscope control
Confocal imaging
The Ni series is sturdy enough for the incorporation of a confocal scanner.
The broad flux of light allows the capture of bright confocal images.
Camera/microscope control
Confocal microscope C2
14
15
16
17
ENG-mount
Zooming
Adapter
ENG-mount
TV Adapter
C-mount
TV Adapter
Relay
Lens
1x
C2
C-mount
TV Adapter
0.35x,
0.45x,
0.6x,
0.7x
NI-DRS
DSC Rotation Stopper
*7
C-mount
Adapter
0.7x
C-mount
TV Adapter A
CFI 12.5x
CFI 10x
CFI 10x M
C-mount
Adapter
0.55x
C-CT
Centering
Telescope
CFI 15x
CFI UW
10x
*6
Relay
Lens
1x
TV Zoom
Lens
NI-RPSAA
DSC Adapter A
C-mount
TV Adapter
VM2.5x
C-mount
TV Adapter
VM4x
C-ISA Analyzer
Tube for Simple Polarization
NI-RPSAB
DSC Adapter B
C-TAQ Tube
Adapter for
Quadrocular Tube
V-T
Photo Adapter
JAPAN
NI-SHCS Motorized
Shutter Cable Short
NI-RPS
DSC Support
Column
A1
Adapter
Y-TV TV
Tube
NI-BAW-E Motorized
Barrier Filter Wheel
NI-SH-E
Motorized
Shutter
NI-AST Analyzer
Slider Stopper
*5 C-TB
Binocular
Tube
NI-RPZ-E Motorized
DSC Zooming Port for
Quadrocular Tube
NI-TT-E Motorized
Quadrocular Tilting Tube
*5 C-TE2
Ergonomic
Binocular Tube
C-TT
Trinocular
Tube T
C-TF
Trinocular
Tube F
NI-TT Quadrocular
Tilting Tube
NI-RPZ
DSC Zooming Port for
Quadrocular Tube
D-ES Slider for
Reected Illumination
D-DA Analyzer
Slider for DIC
NI-ND6-I Intelligent
DIC Sextuple Nosepiece
*7
*4
*2
NI-FLT6 Epiuorescence
Cube Turret
D-FB
D-FB
Excitation Balancer
NI-FLT6-I
Intelligent Epiuorescence
Cube Turret
*1
NI-FLT6-E
Motorized Epiuorescence
Cube Turret
*1
TI-SHAEP-I Motorized
Shutter Adapter for
Inverted Epi-FL
NI-EXW-E
Motorized
Excitation
Filter Wheel
*2
C-HGFIF15/30
HG Fiber
NI-ND6-E
Motorized DIC
Sextuple Nosepiece
NI-N7-I Intelligent
Septuple Nosepiece
NI-N7-E Motorized
Septuple Nosepiece
*1
C-HGFIB HG
100W Adapter R
Hg Lamphouse
HMX-3B
CFI60
Objective Lens
D-FLD Filter
Cube for Darkeld
Illumination
D-LP
Lambda Plate
NI-FLEI EPI-Fluorescence
Attachment
*3
DIC Slider
D-FLE Filter
Cube for Reected
Illumination
NI-SH-E
Motorized
Shutter
NI-SSR Substage
for Rotatable/Motorized
Stage
C-HGFI/HGFIE HG Precentered
Fiber Illuminator Intensilight
Mercury Lamp
Socket S 100W BL
C-FL Epi-
Filter Cube
TI-A DIC
Analyzer
Cube
NI-ND-E
Motorized
ND Filter
D-ND6 DIC
Sextuple Nosepiece
NI-FA FL/
DIC Analyzer
NI-SHAEP-U Motorized
Shutter Adapter
for Upright Epi-FL
Y-TV 0.55
TV Tube
C2
Adapter
C-N Sextuple
Nosepiece
JAPAN
A1
C-T T-C
Trinocular
Tube
CFI UW
10x M
ENG-mount
TV Adapter
0.45x, 0.6x
Projection
Lens
PLI 2x,
PLI 2.5x,
PLI 4x,
PLI 5x
C-mount Camera
ENG-mount Camera
FX-III Series
Photomicrographic
System
C-SHG1
Power Supply
for HG100W
C-FCL Epi-Fl
Collector Lens
NI-SHCS Motorized
Shutter Cable Short
Hg Lamphouse
HMX-4B
RUN TIME
REPLACE BULB
BEFORE THE
SPECIFIED TIME
POWER
LAMP
READY
IGNITION
POWER
0
SUPER HIGH PRESSURE
MERCURY LAMP
POWER SUPPLY
NI-SHADI Motorized
Shutter Adapter for
Dia-Illumination
C-FCL Epi-Fl
Collector Lens
Xe Lamphouse
HMX-4
Xe Lamp
Socket 75W
Xenon Power
Supply 75W
XENON POWER SUPPLY XPS-100
LAMP HOURS
NI-CH
Condenser Holder
NIE-CB
Connector Box
ECLIPSE Ni-E
C-SP Polarizer
for Simple
Polarization
D-DP DIC
Rotatable
Polarizer
Coarse
Achromat
Swing-out
Condenser
2-100x
Darkeld
Condenser
for X/Y (Oil)
1.2
1.0
0.8
0.6
0.4
D 0.95 - 0.80
JAPAN
Darkeld
Condenser
for X/Y (Dry)
C-C Slide
Achromat
Condenser
2-100x
C-C Abbe
Condenser
NA 0.9
X LWD
Condenser
C-C Achromat
Swing-out
Condenser
1-100x
D-CUO DIC
Condenser
Oil
- C Oi l
D
NI-CUD Universal
Condenser Dry
JAPAN
18
MADE IN JAPAN
HMX Lamphouse
POWER
MIN.
MAX.
UN2 Transformer
100W
Z Speed
+X
-Y
C-FCL Epi-Fl
Collector Lens
0.2
D F C
Fine
NI-ERG NI
Ergo Controller
Ex Fine
-X
A-A N.A=1.4
1.4
JAPAN
Coarse
+Y
O 1.43 - 1.20
Ex Fine
Fine
XY Speed
Constant Speed
NI-LH Precentered
Lamphouse
Quartz Epi-Fl
Collector Lens
NI-CUD-E Motorized
Universal Condenser Dry
D F C
POWER
LIGHT
PUSH ON
Halogen
Lamp Socket
100W
C-CTC Camera
Trigger Cable U3/L3
DS-L3 DS
Camera
Control Unit
19
Projection
Lens
PLI 2x,
PLI 2.5x,
PLI 4x,
PLI 5x
C-mount Camera
ENG-mount Camera
FX-III Series
Photomicrographic
System
ENG-mount
TV Adapter
C-mount
Zooming
Adapter
ENG-mount
Zooming
Adapter
C-mount
TV Adapter
Relay
Lens
1x
C-mount
Adapter
0.7x
C-mount
TV Adapter A
NI-DRS
DSC Rotation Stopper
CFI 12.5x
CFI 10x
CFI 10x M
C-mount
Adapter
0.55x
CFI 15x
C-CT
Centering
Telescope
CFI UW
10x
C-LHGFI
HG Lamp
CFI UW
10x M
*5
JAPAN
TV Zoom
Lens
A1
JAPAN
C2
*5
C-mount
TV Adapter
0.35x,
0.45x,
0.6x,
0.7x
Relay
Lens
1x
NI-RPSAA
DSC Adapter A
C-mount
TV Adapter
VM2.5x
C-mount
TV Adapter
VM4x
C-HGFIF15/30
HG Fiber
NI-RPSAB
DSC Adapter B
C-TAQ Tube
Adapter for
Quadrocular Tube
V-T
Photo Adapter
C-HGFIB HG
100W Adapter R
A1
Adapter
Y-TV
0.55
TV Tube
Y-TV
TV Tube
NI-SHCS Motorized
Shutter Cable Short
NI-AST Analyzer
Slider Stopper
C2
Adapter
NI-FA FL/
DIC Analyzer
NI-RPZ-E Motorized
DSC Zooming Port for
Quadrocular Tube
NI-TT-E Motorized
Quadrocular Tilting Tube
NI-TT Quadrocular
Tilting Tube
*4 C-TF
Trinocular
Tube F
NI-SH-E
Motorized
Shutter
NI-FLEI EPI-Fluorescence
Attachment
NI-FLT6-I
Intelligent Epiuorescence
Cube Turret
*1
NI-FLT6-E
Motorized Epiuorescence
Cube Turret
*1
*2
D-FB
D-FB
Excitation Balancer
NI-RPZ
DSC Zooming Port for
Quadrocular Tube
NI-EXW-E
Motorized
Excitation
Filter Wheel
TI-SHAEP-I Motorized
Shutter Adapter for
Inverted Epi-FL
*2
C-TB
Binocular
Tube
*4 C-TT
Trinocular
Tube T
NI-SHAEP-U Motorized
Shutter Adapter
for Upright Epi-FL
*5
NI-FLT6 Epiuorescence
Cube Turret
*4 C-T T-C
Trinocular
Tube
C-HGFI/HGFIE HG Precentered
Fiber Illuminator Intensilight
NI-BAW-E Motorized
Barrier Filter Wheel
NI-RPS
DSC Support
Column
*3
*1
NI-AMSS Arm Spacer 49mm
C-TE2
Ergonomic
Binocular Tube
D-FLD Filter
Cube for Darkeld
Illumination
D-FLE Filter
Cube for Reected
Illumination
TI-A DIC
Analyzer
Cube
C-FL Epi-
Filter Cube
NI-SH-E
Motorized
Shutter
Objective
Slider
Fiber Illuminator
NI-FIA Fiber
Illumination Adapter
NI-SHCS Motorized
Shutter Cable Short
DIC Slider
CFI75
Objective Lens
DIC Slider
CFI60
Objective Lens
NI-SA Motorized
XY Stage Adapter
NI-SHADI Motorized
Shutter Adapter for
Dia-Illumination
ECLIPSE Ni-E
FN-C LWD
Condenser
NIE-CB
Connector Box
NI-SSF Substage
for Focusing
Nosepiece
Coarse
Ex Fine
Coarse
Fine
XY Speed
Constant Speed
Fine
NI-LH Precentered
Lamphouse
NI-ERG NI
Ergo Controller
Ex Fine
+Y
-X
N 1 Dr
NI-IRLH Precentered IR
Lamphouse
Z Speed
+X
-Y
JAPAN
FN-PT4X 4X
Support Lens
D-C DIC N1
DRY DIC Module
NI-PT Polarizer
Turret
D-C DIC N2
DRY DIC Module
FN-3PS2 FN1
Standard Stage
FN-IRP IR Ploarizer
FN-P Polarizer
NI-S-E Motorized
XY Stage
NI-SH-D
Dish Holder
20
NI-CTLA
Control Box A
DS-L3 DS
Camera
Control Unit
C-CTC Camera
Trigger Cable U3/L3
21
Projection
Lens
PLI 2x,
PLI 2.5x,
PLI 4x,
PLI 5x
C-mount Camera
ENG-mount Camera
ENG-mount
TV Adapter
C-mount
Zooming
Adapter
ENG-mount
Zooming
Adapter
C-mount
TV Adapter
0.35x,
0.45x,
0.6x,
0.7x
C-mount
TV Adapter
Relay
Lens
1x
*8
CFI 10x
CFI 10x M
CFI 15x
C-CT
Centering
Telescope
C-TAQ Tube
Adapter for
Quadrocular Tube
Relay
Lens
1x
C-mount
TV Adapter
VM2.5x
CFI UW
10x M
CFI UW
10x
JAPAN
TV Zoom
Lens
CFI 12.5x
C-mount
Adapter
0.55x
C-mount
Adapter
0.7x
C-mount
TV Adapter A
NI-DRS
DSC Rotation Stopper
*7
C-mount
TV Adapter
VM4x
NI-RPSAA
DSC Adapter A
NI-RPSAB
DSC Adapter B
Y-THPL LED
Pointer Unit
for Teaching Head
V-T
Photo Adapter
C2
JAPAN
TH-AC2 AC Adapter
Y-TV
0.55
TV Tube
Y-TV
TV Tube
NI-FA FL/DIC
Analyzer
NI-RPS
DSC Support
Column
C2
Adapter
NI-AST Analyzer
Slider Stopper
*8
NI-SHCL
Motorized Shutter
Cable Long
*6 C-TB
Binocular
Tube
C-T T-C
Trinocular
Tube
NI-FLT6
Epi-uorescence
Cube Turret
*6 C-TE2
Ergonomic
Binocular Tube
C-TT
Trinocular
Tube T
C-TF
Trinocular
Tube F
*1
*2
NI-RPZ
DSC Zooming Port for
Quadrocular Tube
D-FB Excitation
Balancer
NI-FLT6-I
Intelligent
Epi-uorescence
Cube Turret
*2
NI-PAU
Ni Photoactivation Unit
NI-FLT6-E
Motorized Epiuorescence Cube Turret
4
NI-ND6-I Intelligent
DIC Sextuple Nosepiece
NI-ND6-E
Motorized DIC
Sextuple Nosepiece
NI-N7-I Intelligent
Septuple Nosepiece
NI-N7-E Motorized
Septuple Nosepiece
*3
C-FL Epi-
Filter Cube
D-DA Analyzer
Slider for DIC
DIC Slider
D-FLD Filter
Cube for
Darkeld
Illumination
CFI60
Objective Lens
D-LP
Lambda Plate
NIU-CSRR2
Right Handle Rotatable
Ceramic-coated Stage
with Holder
D-ND6 DIC
Sextuple Nosepiece
C-LHGFI HG Lamp
NI-SHAEP-U
Motorized
Shutter Adapter
for Upright Epi-FL
NI-FLEI EPI-Fluorescence
Attachment
*4
NI-TT Quadrocular
Tilting Tube
C-N Sextuple
Nosepiece
Y-THSP Support
for Side by Side
D-FLE Filter
Cube for
Reected
Illumination
C-HGFIB HG 100W
Adapter R
NI-SSR Substage
for Rotatable/
Motorized Stage
NI-SH-E
Motorized Shutter
C-HGFIF15/30
HG Fiber
TI-A DIC
Analyzer Cube
NI-SHCL Motorized
Shutter Cable Long
C-HGFI/HGFIE HG
Precentered Fiber
Illuminator Intensilight
Hg Lamphouse
HMX-3B
Mercury Lamp
Socket S 100W BL
C-FCL Epi-Fl
Collector Lens
Hg Lamphouse
HMX-4
NI-SS Substage
C-CSR Right
Handle Ceramic-coated
Stage
C-SR2S
Right Handle Stage
with 2S Holder
NI-SHADI Motorized
Shutter Adapter for
Dia-Illumination
NI-CH
Condenser Holder
C-CSR1S Right
Handle Ceramic-coated
Stage with 1S Holder
C-SHG1
Power Supply
for HG100W
NI-LH Precentered
Lamphouse
ECLIPSE Ni-U
C-H2L Specimen
Holder 2L
TI-100WRC 100W
Lamphouse
Remote Cable
C-H1L Specimen
Holder 1L
NI-CALN1 2-4X Auxiliary Lens
for N1 Position
D-C 2-4X Auxiliary Lens
C-C Phase
Contrast Turret
Condenser
Achromat
Swing-out
Condenser
2-100x
Darkeld
Condenser
for X/Y (Oil)
Darkeld
Condenser
for X/Y (Dry)
C-C Slide
Achromat
Condenser
2-100x
22
C-C Abbe
Condenser
NA 0.9
X LWD
Condenser
C-C Achromat
Swing-out
Condenser
1-100x
D-CUO DIC
Condenser
Oil
D-C DIC
Module Oil
NI-CUD Universal
Condenser Dry
C-SP Polarizer
for Simple
Polarization
D-DP DIC
C-TP Polarizer
Rotatable
for First-order
Red Compensation Polarizer
NI-CTLB
Control Box B
Xe Lamphouse
HMX-4
Xe Lamp
Socket 75W
*5
C-CTC Camera
Trigger Cable
U3/L3
Quartz Epi-Fl
Collector Lens
C-FCL Epi-Fl
Collector Lens
C-CTC Camera
Trigger Cable
U3/L3
*5
Xenon
Power Supply
75W
HMX Lamphouse
UN2 Transformer
100W
DS-L3 DS
Camera Control Unit
C-FCL Epi-Fl
Collector Lens
NI-SRCP
Simple Remote
Control Pad
Halogen Lamp
Socket 100W
23
Specifications
Ni-E
Optical system
Focusing
(stroke from focus
point)
Ni-U
Ni-E
Manual
6 filter cubes mountable, High S/N noise terminator mechanism for all turrets
NI-FLT6-E Motorized Epi-fluorescence Cube Turret
Motorized shutter, Status check function*3
NI-FLT6-I Intelligent Epi-fluorescence Cube Turret
Manual shutter, Status check function*3
NI-FLT6 Epi-fluorescence Cube Turret
Manual shutter
Light distribution
device
Option
Controls
Tubes
F.O.V. 25 mm*1
(Eyepiece/Port)
CFI 10x (22) CFI 10xM Photomask (22) CFI 12.5x (16)
CFI 15x (14.5) CFI UW10x (25) CFI UW10xM Photomask (25)
C-TB Binocular Tube
C-TE2 Ergonomic Binocular Tube (100/0, 50/50 with C-TEP2 DSC Port)
Inclination angle: 10-30 degree, Extension up to 40 mm
C-TF Trinocular Tube F (100/0, 0/100)
C-TT Trinocular Tube T (100/0, 20/80, 0/100)
C-TT-C Trinocular Tube (100/0, 0/100, for confocal)
NI-TT Quadrocular Tilting Tube (Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100)
Inclination angle: 15-35 degree
Motorized
Intelligent
Nosepieces
Manual
Power consumption
211W
(with max. halogen lamp intensity
and full motorized options)
Weight (approx.)
29 kg
(Epi-fluorescent configuration with motorized
quadrocular tilting tube)
FN-S2N Sliding Nosepiece
(for CFI60 objectives)
Changeover 2 objectives, DIC slider insertable
FN-MN-H Single Objective Holder
(for CFI75 objective)
DIC slider insertable
FN-3PS2 FN1 Standard Stage
Cross travel 30(X) x 30(Y) mm
Stages
NI-S-E Motorized XY Stage
Resolution: 0.1 m
NI-SH-D Dish Holder (option)
Substages
NI-SSR Substage
(for Motorized Universal Condenser and
Rotatable/Motorized stages)
24
Ni-U
96W
(with max. halogen lamp intensity
and full motorized options)
42 kg
(Photoactivation configuration with motorized
quadrocular tilting tube)
20 kg
(Brightfield configuration with ergonomic
binocular tube)
*1 When used with a double layer, such as with layered epi-fluorescence cube turrets, F.O.V. is 22.
*2 Can only be mounted on the NI-SS Substage
*3 Status check function: Status of Filter/Nosepiece etc. can be recorded with captured images and/or displayed on the controller monitor.
25
Dimensional diagram
Total Power
500mW MAX.
CW 400700nm
IEC/EN60825-1:2007
604
JAPAN
389
60
FN-S2N
205
A.S
320
437
130
Ni-U
Configured with a motorized epi-fluorescence cube turret
and quadrocular tilting tube
205
340
60
488
320
383
130
383
130
Ni-U
205
340
60
501
320
Unit: mm
26
27
Company names and product names appearing in this brochure are their registered trademarks or trademarks.
N.B. Export of the products* in this brochure is controlled under the Japanese Foreign Exchange and Foreign Trade Law.
Appropriate export procedure shall be required in case of export from Japan.
*Products: Hardware and its technical information (including software)
Monitor images are simulated.
WARNING
Specifications and equipment are subject to change without any notice or obligation on the part
of the manufacturer. September 2011 2011 NIKON CORPORATION
NIKON CORPORATION
Shin-Yurakucho Bldg., 12-1, Yurakucho 1-chome, Chiyoda-ku, Tokyo 100-8331, Japan
phone: +81-3-3216-2375 fax: +81-3-3216-2385
http://www.nikon.com/instruments/
NIKON UK LTD.
NIKON BELUX
NIKON AG
SWITZERLAND phone: +41-43-277-28-67 fax: +41-43-277-28-61
This brochure is printed on recycled paper made from 40% used material.
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