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Food Control
journal homepage: www.elsevier.com/locate/foodcont
Sensory quality control for food certication: A case study on wine. Panel
training and qualication, method validation and monitoring
I. Etaio, M. Albisu, M. Ojeda, P.F. Gil, J. Salmern, F.J. Prez Elortondo *
Laboratorio de Anlisis Sensorial Euskal Herriko Unibertsitatea (LASEHU), Facultad de Farmacia, Universidad del Pas Vasco Euskal Herriko Unibertsitatea,
Unibertsitateko Ibilbidea Paseo de la Universidad, 7, 01006 Vitoria-Gasteiz, Spain
a r t i c l e
i n f o
Article history:
Received 9 December 2008
Received in revised form 31 July 2009
Accepted 8 August 2009
Keywords:
Sensory quality control
Method validation
Panel training and monitoring
Method accreditation
a b s t r a c t
The use of qualied panels applying specic evaluation methods for sensory quality control increases the
reliability of the results, even more if the method is accredited by ofcial accreditation bodies.
Based on the work developed with young red wine, this paper describes all the steps carried out after
having dened the method: assessor selection, basic and specic training, qualication of assessors (individual performance checking) and method validation (panel performance checking). Other procedures
necessary to demonstrate technical competence in a permanent way (monitoring of panel and assessors
at each session, quality controls and assessor re-qualication) are also described.
Due to the scarce references in sensory method accreditation, many of the tests and criteria proposed
are original. They could be helpful for other laboratories dealing with sensory quality control of food
products, especially those with quality distinctiveness labels.
2009 Elsevier Ltd. All rights reserved.
1. Introduction
The development of methods to assess in a reliable way the sensory quality of specic foods, mainly products with Protected Designation of Origin (PDO), in order to certicate them is a pressing
need (Feria-Morales, 2002). In this sense, the importance of laboratory accreditation will probably increase over the next years
(Gacula, 2003).
After method denition and before starting product assessment
in a systematic way, several steps have to be carried out: assessor
selection, basic and specic training, assessor qualication and
method validation. Only when a method provides reliable results
(repeatability, reproducibility and discrimination ability) it can
be accredited according to ISO 17025 (ISO, 2005a).
To prove that technical competence remains through the time, a
continuous monitoring of assessors and panel, assessor re-qualications, as well as periodical quality controls are necessary.
Regarding assessor selection and training, there are many standards and publications (ASTM, 1981, 2003; Bressan & Behling,
1977; International Olive Oil Council (IOOC), 2007; ISO, 1993,
2005b, 2006, 2008; Issanchou, Lesschaeve, & Kster, 1995; Lyon,
2002; Muoz, Civille, & Carr, 1992). There are also many reports
for panel performance measurement (Brien, May, & Mayo, 1987;
Brcenas, Prez Elortondo, & Albisu, 2000; Findlay, Castura,
Schlich, & Lesschaeve, 2006; King, Hall, & Cliff, 2001; Kwan &
Kowalski, 1980; Latreille et al., 2006; Rossi, 2001; Scaman & Dou,
2001), usually referred to numerical scores. However, references
for training and checking panels and methods in specic food
product evaluation are very scarce (Prez Elortondo et al., 2007;
Torre, 2002), so often internal procedures have to be developed
in each laboratory.
Having dened the method to evaluate the sensory quality of
Rioja Alavesa (RA) young red wines (Etaio et al., 2010), this work
shows the approach and criteria followed for assessor selection,
basic training, specic training, assessor qualication and method
validation. It also presents the criteria for continuous monitoring
of the panel and individual assessors, as well as quality controls
carried out throughout the systematic evaluation of wine samples,
once the method was accredited in February 2008 (http://
www.enac.es).
The innovative approaches and criteria described could be very
useful for other laboratories needing to implement sensory methods for the evaluation of specic foods, not only for PDO products,
but also for other products with quality distinctiveness labels.
2. Materials and methods
2.1. Assessors
The number of assessors participating in each of the steps explained in this work varied due to three main reasons: (i) some
minimum requirements had to be overcome in each step to pass
onto the next one; (ii) some assessors left the panel during the
543
Table 1
Results of selection tests.
Testa
Reference
Ishihara test
ISO (1991)
ISO (2004a)
ISO (2004b)
Ranking test
of colour
of odour
of taste
of texture
ISO (1988)
Description test
of odour
of texture
ISO (1993)
% of passesb
94.4
33.3
96.3
88.9
100.0
77.8
55.6
83.3
64.8
96.3
544
Table 2
Results of basic training tests.
Test
Reference
% of passesa
ISO (1993)
ISO (1983)
95.8
ISO (2004b)
ISO (2003)
ISO (2003)
ISO (1985)
ISO (1994)
79.2
95.8
83.3
41.7
87.5
87.5
95.8
87.5
100
91.7
95.8
Test repetition of non-passed test by assessors not reaching the established 75%
are not considered in this table.
545
Calculations
ide
100
Odour reference identification No ref
20
No ref ide
100
Aroma reference identification
20
ide
100
Imbalance reference identification No ref
10
ide
Total reference identification No ref
100
50
t-Student test (with data from A and B wines from all the
assessors and from the two sessions as a
whole) ? parameters with signicant differences were
discriminative
j
xA
xB j
CI p
2
2
I1 I2
SDR SDs1wASDs2wASDs1wBSDs2wB
4
SDR: standard deviation in repeatability. Calculated for each
sensory parameter and for each assessor. Average of the
standard deviations (SD) in the two sessions (s1 and s2)
considering only the wines evaluated in triplicate (wA and wB)
p
SDRr Var s2 Var rep2
SDRr: standard deviation of reproducibility. Calculated for
each sensory parameter and for each assessor. Only wines
evaluated in triplicate (A and B) were considered
Var s: variance between sessions. The standard deviation of
the standard deviations of the two sessions.
Var rep: variance of repeatability. It is the average of the
standard deviations of the two sessions
att
CF Max Cit
cit att
pos
1000
CF: citation frequency. Calculated for the panel and for each
assessor
Cit att: times that one attribute is cited in a wine
Max cit att pos: maximum times that this attribute can be
cited in this wine
For each wine evaluated in triplicate in both 2nd and 3rd
sessions (wines A and B) the six samples were computed
together. For each wine evaluated once in each session (C
and D) the two samples were computed together
Attribute/defect identication was calculated separately for
odour and for aroma. Each attribute was linked to the
respective wine
546
Table 4
Parameters studied in method validation and criteria to pass each test.
Studied parameter
Repeatability in scores: ability to give the same or similar scores when the same
wine is evaluated in replicate in the same session
Reproducibility in scores: ability to give the same or similar scores when the same
wine is evaluated in replicate in different sessions
Citation difference among the replications of the same wine in the same session
must be 62 for at least 80% of the attributes with a citation frequency (CF) P50%
The two sessions are considered separately. There are considered only the
attributes with a CF P50% (4 of 7 assessors citing it) in any of the three
replications of wine A or B in a session
Citation difference for an attribute in the same wine between sessions 1 and 2
must be 66 for at least 80% of the attributes with a CF P50%
Only the attributes with a CF P50% in at least one of the two sessions are
considered (attributes cited at least 11 times in a wine, since the maximum
citation times would be: 3 replications 7 assessors = 21)
The number of discriminative parameters in the second session must not be <50%
or >150% of the discriminative parameters in the rst session
The sensory parameters discriminative between A and B wines are determined by
the t-Student test
Table 5
Attribute citation results for validation in repeatability and reproducibility in attribute identicationa.
Attribute
Wine A
Attribute
Citations in session 1
Rep1
Citations in session 2
Wine B
Citations in session 1
Rep2
Rep3
Total
Rep1
Rep2
Rep3
Total
Odour complexity
Ripe fruit
6
Licorice
4
Forest berry 4
Herbaceous
3
6
3
4
4
7
5
4
5
19
12
12
12
7
3
6
3
5
4
2
2
3
2
1
2
15
9
9
7
Aroma complexity
Ripe fruit
6
Licorice
5
Forest berry 3
6
4
3
6
5
5
18
14
11
7
4
4
5
4
3
4
3
3
Citations in session 2
Rep1
Rep2
Rep3
Total
Rep1
Rep2
Rep3
Total
Ripe fruit
Un-determined fruit
Smoky
Herbaceous
2
5
2
4
2
5
2
3
4
3
1
4
8
13
5
11
2
4
1
3
2
5
4
2
3
4
2
3
7
13
7
8
16
11
10
Un-determined fruit
Herbaceous
4
2
5
3
4
3
13
8
3
3
4
5
5
3
12
11
Acidity excess
Only attributes with a citation frequency P50% in at least one of the repetitions (four or more citations) are included.
The number of cases that this requirement is not fullled (considered a deviation) must be lower than 15% of total cases (product of
multiplying the number of samples by eight parameters), as proposed by Prez Elortondo et al. (2007).
If panel deviation occurred the wines with a higher number of
standard deviations equal to or higher than 1 were evaluated again
in the next session, and the results checked again.
Regarding the results of panel monitoring, in eight sessions
(40%) the standard deviations equal or higher than 1 exceed 15%
of total cases. When the most problematic wines were tasted again
in the next session, results fullled the established requirements.
Parameters with the most cases of standard deviation equal to or
higher than 1 were odour complexity (30.6% of the wines), aroma
complexity (24.8%), global aroma persistence (22.3%) and balance
and body (21.5%). Parameters with the least number of panel deviations were colour intensity (2.5% of the wines), colour hue (5.0%),
aroma intensity (14.0%) and odour intensity (15.7%).
547
Session 1
Session 2
Citations in
wine A
Citations in
wine B
Discriminative?
Citations in
wine A
Citations in
wine B
Discriminative?
19
2
12
12
12
8
13
5
5
11
YES
YES
YES
YES
NO
15
6
9
9
7
7
13
2
1
8
YES
YES
NO
NO
NO
YES
YES
NO
NO
Aroma attributes
Ripe fruit
18
Un-ripe fruit
3
Licorice
14
Forest berries
11
Herbaceous
8
8
13
7
3
8
YES
YES
YES
YES
NO
16
5
11
10
7
9
12
4
1
11
YES
YES
YES
NO
YES
YES
YES
YES
NO
NO
Odour attributes
Ripe fruit
Un-ripe fruit
Licorice
Forest berries
Herbaceous
Attribute discriminative in
session 1 and in session 2
Only attributes with a citation frequency P50% in at least one of the sessions (11 or more citations) are included.
548