Beruflich Dokumente
Kultur Dokumente
Abstract
This study evaluates the hematological response of rainbow trout fingerlings to crowding stress induced by high
stocking densities, during a 33 days period. A number of 254 rainbow trout, with an average weight of 29.51 1.32
g, were divided into four rearing units in order to create different stocking densities: in DS1-2.64 kg/m3, DS2-5.16
kg/m3, DS3-7.12 kg/m3 and in DS4-9.42 kg/m3. To determine the leukograma and also the absolute number of
leukocytes, blood samples were collected after the experimental trial and used immediately to make smears.
Physiological stress induced by maintenance under different stocking densities is reflected by rainbow trout
leukograma. We can notice a decrease of absolute number of leukocytes, with direct implication of stocking density
level, from 69.7910.22 (x103cel./mm3) in DS1 to 46.349.83(x103cel./mm3) in DS4. In this experiment, the immune
(defense) system has been influenced by stocking density, the general reduction of leukocytes number being a
characteristic response to stressful conditions.
Keywords leukograma, rainbow trout, recirculating aquaculture system, stocking density
1. Introduction
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Cristea V. et. al./Scientific Papers: Animal Science and Biotechnologies, 2012, 45 (2)
Number of fish
Initial biomass (g)
Mean individual weight (g/fish)
Initial stocking density (kg/m3)
Final numbers of fish
25
792
31.68
2.64
25
51
1550
30.39
5.16
50
75
2138.94
28.52
7.12
75
DS4
103
2828.20
27.46
9.42
103
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Cristea V. et. al./Scientific Papers: Animal Science and Biotechnologies, 2012, 45 (2)
Figure 1-4. Morphology of circulating blood cell of the rainbow trout, 10 oc x 100 ob, MGG staining: Ererythocytes, L-lymphocytes, M- monocyte, N-neutrophils, T-trombocytes
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Cristea V. et. al./Scientific Papers: Animal Science and Biotechnologies, 2012, 45 (2)
Figure 5. The leukograma of rainbow trout held under different stocking densities
Table 2. Changes in absolute values of the leukocyte series during experimental period
Cell type
Leukocytes
(x103cel./mm3)
Lymphocytes
(x103cel./mm3)
Monocytes
(x103cel./mm3)
Neutrophils
(x103cel./mm3)
Trombocytes
(x103cel./mm3)
DS1(26)
DS2(52)
DS3(76)
DS4(103)
AvgSD
69.7910.22
69.6217.46
54.5617.12
46.349.83
AvgSD
68.2410.25
68.2417.21
53.0617.29
44.809.64
AvgSD
0.160.22
0.090.16
0.180.24
0.240.30
AvgSD
1.40.21
1.280.59
1.320.16
1.300.58
AvgSD
2.503.54
7.932.22
9.381.65
8.092.27
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Cristea V. et. al./Scientific Papers: Animal Science and Biotechnologies, 2012, 45 (2)
4. Conclusions
The hematological assessment of intensively
farmed fishes is an integral part of their health
status evaluation. In conclusions, the results of the
present study shows that rainbow trout held at
high stocking densities appear to be experiencing
chronic stress, as indicated by the major changes
in the leukocyte system. A slight trend of reducing
the lymphocytes with increasing stocking density
can be observed. This demonstrates that stress
inducing by high stocking densities does change
the proportion and number of fish blood
leukocytes. The primary consequence of a low
number of lymphocyte is immunosuppresion,
resulting an increased susceptibility to desease.
Further experiments are required for studying
whether high stocking densities affects the ability
of fish to resist to additional stressors.
Acknowledgements
The work of Mirela (CRETU) MOCANU was
supported by Project SOP HRD TOP ACADEMIC
76822/2010 and the work of Sandita (ION)
PLACINTA and Marian Tiberiu COADA was
supported by Project SOP HRD EFFICIENT
61445/2009
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