Beruflich Dokumente
Kultur Dokumente
Basal media
Soft agar tube For keeping stock cultures by stab
Autoclave at 110°C for 10 minutes
(in small tube) inoculation.
To demonstrate motility of organism.
*both with cotton-wool stoppers.
Lihatlah pd manisnya kejayaan, bukan pd peritnya usaha: Husnun Nisak Binti Hamdan =)
(nutrient agar+ sterile defibrinated Differential medium: differentiating org.
blood) Autoclave at 120° for 20 minutes According to their haemolytic action on
blood.
Chocolote agar Enriched media Culture of Haemophilus & Neisseria
(nutrient agar+ sterile defibrinated Autoclave at 120° for 20 minutes
blood+ heated to 80°C for 10 min)
Lihatlah pd manisnya kejayaan, bukan pd peritnya usaha: Husnun Nisak Binti Hamdan =)
(peptone water+agar+bile
salt+lactose+neutral red(phenol Steaming for 30-60 min (autoclave)
red).
Sodium taurocholate(bile salt)
*selective agent or inhibitory substance:
- growth of gram +ve bacteria
Indicator-phenol red
*pinkfermenting colonies
*yellownon-fermenting colonies
Sugar media( peptone water, 0.5- Sugar media Placed in tubes containing:
1.0% sugar:) inverted Durham’s tubes
Glucose Indicatorsphenol red, to trap air bubbles in case of
Maltose Change their color w change of pH fermentation with gas.
Mannitol Yellowacid pH An indicator media to differentiate
Lactose Redalkaline pH bacteria according to fermentation
Sucrose Tyndallisation for 30 min for 3 of sugars.
successive days.
Lihatlah pd manisnya kejayaan, bukan pd peritnya usaha: Husnun Nisak Binti Hamdan =)
Lihatlah pd manisnya kejayaan, bukan pd peritnya usaha: Husnun Nisak Binti Hamdan =)