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QUESTIONS

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What is anticoagulant?(3)
Write the name of anticoagulant with their use in various labs.(3)
Describe in detail how to perform urine detail examination (urine analysis).
Write down microscopic finding in urine D/R.
What do you mean by hepatitis?
Describe the different methods used in laboratory for the performance of test.
Name the different media used for detection of bacteria in urine, stool, and pus and blood culture.
(2)
How will you collect the sample of blood for culture and sensitivity?
Describe the procedure of any two:
Widal test(2)
Cholesterol
Blood sugar
Write down the manual method of TLC (total leucocytes count) estimation.
Draw the diagram of different White Blood Cells(2)
Describe the method of Glucose estimation by colorimeter or spectrometer (2). Write the normal
range of fasting blood sugar and random blood sugar.
Describe the method of collecting urine for culture in adult male. How you will process the urine
culture specimen in your laboratory.
Write down the steps of ZN staining method in detail and draw the diagram of Acid Fast Bacilli
(AFB).
Describe the urine detail report (urine D/R) write down the chemical method for detection of
sugar in urine.
Write down the manual method of Hemoglobin estimation.
Describe the manual method of platelet count.(3)
Describe the method of ALT (SGPT) in your lab and write down the reference range.
Describe the methods of collection of blood culture in a febrile patient admitted in medical
I.C.U.how you will proceed the blood culture specimen in your laboratory.
Write down the steps of gram staining method in detail(2) and name the most common gram
positive and gram negative organism.
Describe the urine detail report (urine D/R).explain physical, chemical and microscopic
examination.
What is CBC? (2)Write the parameters of CBC and their normal ranges in adult male.
Name the cardiac enzymes? How will you perform the test?
Name the different serological tests.
How will you collect and process the blood culture for the investigation of an adult male coming
with history of fever.
What do you mean by Stool D/R?
How will you perform the occult blood test in stool?
Write the normal values of following:
(a)RBS (b)Urea (c)Creatinine (d)Cholesterol (e)ALT (f)Aibumin (g)CK (h)Calcium (i)HCO3
Name common microbiological culture media.
How would you collect a 24 hours urine sample? Name the tests done on 24 hour urine sample.
Draw the picture of neutrophil, lymphocyte, eosinophil, monocyres and platelet.
What are parasites commonly found in stool and blood on microscopic examination. Draw their
diagram.

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Chemical method to collect sugar in urine.


Dengue serology.
Coombs test
Blood group
Pregnancy test(2)
RA factor(4)
ZN stain(2)
M.T(2)
LFT(2)
Prothrombin time
ESR(3)
MP(4)
Reticulocyte count(2)
Gram Staining
Typi dot(2)
Dengue test(2)
Hand washing in laboratory(2)
Sterilization
Biosaftey
APTT
Widal test
ASOT
Disinfectant
Autoclaves role in sterilization
PT & PTT
Serum & plasma
PCR
Parts of microscope
Sterilization by heat