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M.Sc. entrance class Chapter Test:
10 Cell and Molecular Biology
All50questionsarecompulsoryandcarryequalmarks

Full Marks: 100

1. Ahumancellcontaining22autosomesandaXchromosomeis
a) Asperm
b) Anegg
c) anyoftheabove
d) SkeletalCell
2. IftheDNAcontentofadiploidcellintheG1phaseofthecellcycleisX,thentheDNAcontent
ofthesamecellatmetaphaseofmeiosisIwouldbe
a) x
b) 2x
c) 0.5x
d) 0.25x
3. Howmanydifferentcombinationsofmaternalandpaternalchromosomescanbepackagedin
gametesmadebyanorganismwithadiploidnumberof8(2n=8)?
a) 2
b) 4
c) 8
d) 16
4. Homologouschromosomesmovetowardoppositepolesofadividingcellduring
a) Mitosis
b) MeiosisI
c) MeiosisII
d) Fertilization
5. MeiosisIIissimilartomitosisinthat
a) Sisterchromatidsseparateduringanaphase
b) Thedaughtercellsarediploid
c) Homologouschromosomessynapse
d) DNAreplicatesbeforethedivision
6. MitochondrialDNAisadvantageousforevolutionarystudiesbecause:
a) Itisinheritedonlythroughthefemaleparentandthusevolvesinawaythatallowstress
ofrelationshiptobeeasilyconstructed
b) ItisinsertedintotheXchromosomes
c) Itevolvesmoreslowlythanthegenesinthenucleus
d) Itfirstappearedinhumansandisnotfoundinotheranimals
7. Whichofthefollowingaresimilarbetweentranscriptioninprokaryotesandeukaryotes?
a) RNApolymeraseproducesmRNAswhichgrowinthe53direction
b) RNApolymerasebindtoribosomestoallowtranscription
c) Apolytailisaddedtothe3endofmessengerRNAs
d) Intronsarepresentingeneswhicharesplicedoutaftertranscription
8. Translationinvolves
a) Mappinggenesinbacteriausingaviralcarrier
b) ReadingaDNAstrandandmakinganmRNAcopy
c) TakingupDNAintoacellandchangingitsgeneticmakeup
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9.

10.

11.

12.

13.

14.

15.

16.

17.

18.

d) ReadinganmRNAtoyieldanaminoacidsequenceinaprotein
Thenormalfunctionofapromoteristo
a) Bindthesmallsubunitoftheribosome
b) ServeasanoriginofDNAreplication
c) ServeasanacceptorfortransferRNA
d) ServeasabindingsiteforRNApolymerase
TheprocessofDNAreplicationinvolves:
a) Multipleoriginsofreplicationperchromosomeineukaryotes
b) Bindingofribosomestooriginsofreplication
c) Continuoussynthesisonbothstrandsofthedoublehelix
d) Conservativereplication,withoneoriginaldoublehelixandonetotallynewdoublehelix
asproducts
Whichofthefollowingbiomoleculehasselfrepairmechanisms?
a) DNA,RNAandprotein
b) DNAandRNA
c) DNAonly
d) DNAandproteins
Whichofthefollowingchemicalsinducedepurination
a) methylethanesulphonate(MES)
b) nitrosoguanidine
c) ethylethanesulphonate(EES)
d) allofthese
Which of the following mechanism of DNA polymerase helps in preventing error during DNA
replication
a) rechecking
b) proofchecking
c) proofreading
d) allofthese
Theproteinsinvolvedinmismatchrepairare
a) MutS
b) MutH
c) MutL
d) allofthese
Whichofthefollowingdimerformationismostcommon
a) thymidinedimer
b) cytidinedimer
c) bothaandb
d) noneofthese
Dimerrepairmechanisminclude
a) excisionrepair
b) photoreactivation
c) recombinationalrepair
d) allofthese
Thyminedimersareoftencorrectedbylightinducedrepairmechanism.Theenzymeinvolvedin
theprocessis
a) photolyase
b) photoligase
c) DNAglycosylase
d) Allofthese
Whichofthefollowingisdarkrepair

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19.

20.

21.

22.

23.

24.

25.

26.

27.

a) nucleotideexcisionrepair(NER)
b) baseexcisionrepair(BER)
c) bothaandb
d) noneofthese
DNAglycosylaseisanenzymeinvolvedinbaseexcisionrepair.Thefunctionis
a) additionofcorrectbase
b) additionofcorrectnucleotide
c) removalofincorrectbase
d) removalofphosphodiesterbond
Recombinationalrepairisoftendueto
a) incorporationofmanyincorrectnucleotidesbyDNApolymerase
b) manycytidinedimerandassociatedlargegapsinastrand
c) manythymidinedimerformationandassociatedlargegapsinastrand
d) allofthese
WhichistheDNApolymeraseinvolvedinBER
a) DNApolymerase
b) DNApolymerase
c) DNApolymerase
d) DNApolymerase
Whichofthefollowingisabypassrepairsystem
a) BER
b) NER
c) SOS
d) Recombinationalrepair
DNArepairmechanismisabsentin
a) nucleargenome
b) mitochondrialgenome
c) chloroplastgenome
d) bothbandc
umuC,umuDgenefamilyandRecAproteinsareinvolvedin
a) BER
b) NER
c) SOSrepair
d) Recombinationalrepair
ThedistortioninDNAhelixduetopyrimidinedimerformationiscalledas
a) nick
b) singlestrandedbreaks
c) kink
d) noneofthese
.WhatprovidestheenergyforDNApolymerization?
a) ThehydrolysisofATP(releasingPi)
b) ThehydrolysisofGTP(releasingPi)
c) Thehydrolysisofincomingnucleosidetriphosphates(releasingPPi)
d) None
Replication origins typically consist of a small stretch of DNA that is relatively easy to open.
Whichstatementistrue?
a) ReplicationoriginsarerichinAandTnucleotides.
b) ReplicationoriginsarerichinGandCnucleotides.
c) ReplicationoriginshaveequalnumbersofA,C,G,andTnucleotides.
d) None

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28. DNAreplicationrequirestwoprimers:oneprimerforthelaggingstandandoneprimerforthe
leadingstrand.
a) True
b) False
29. Copyingerrorsnotcaughtbythereplicationmachinerycanbecorrectedby:
a) RNApolymerase.
b) theDNAMismatchRepairSystem.
c) DNAtelomerase.
d) DNAmaintenancemethyltransferase.
30. In addition to its role in DNA repair, homologous recombination is also responsible for
generatinggeneticdiversityduringwhatprocess?
a) Mitosis
b) Meiosis
c) Independentassortmentofchromosomes
d) both(a)and(b)
31. HomologousrecombinationoccursonlybetweenDNAmoleculesthatareidenticalinnucleotide
sequence.
a) True
b) False
32. TheenzymeresponsibleforinitiatingDNAreplicationinprokaryotesis:
a) DNApolymeraseI
b) DNApolymeraseIII
c) DNAPolymerase
d) Primase
33. Antibiotics such as Ciprofloxacin and Flouroquinolines work by inhibiting a specific enzyme
which is normally necessary to relieve torsional strain that is caused by the unwinding of the
helix.Whatisthenameofthisenzyme?
a) DNAligase
b) Topoisomerase(DNAGyrase)
c) singlestrandedbindingprotein
d) primase
34. Whatiscalledabortiveinitiation?
a) Afterinitiationthereisatendencytoproducetruncatedtranscripts
b) PriductionofSmallRNA
c) Initiationfactoraccumulation.
d) Failtoaccumulateinitiationfactor
35. BacterialtranscriptioninitiationrequiresGeneralTranscriptionFactor(GTF)includes
a) TFllD,TFllA
b) IF1andIF2
c) SigmaFactor
d) RhoFactor
36. AllofthefollowingareproteinswithinthecorenucleosomeparticleEXCEPT
a) H1
b) H2B
c) H3
d) H4
37. Asilentmutationinageneresultsin
a) nochangeinthenucleotidesequenceofthemRNAofthegene
b) nochangeintheaminoacidsequenceoftheproteinofthegene
c) noexpressionoftheproteinofthegene
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d) significanteffectonthefunctionoftheproteinthegene
38. Xeroderma pigmentosum, or XP, is an autosomal recessive genetic disorder of DNA repair in
which the ability to repair damage caused by ultraviolet (UV) light is deficient. Which type of
repairsystemisabsentinsuchindividual?
a) NucleotideExcisionRepair
b) BaseExcisionRepair
c) SOS
d) MismatchedRepairSystem
39. Apreviouslyunknownorganismthatlacksanuclearmembraneandmitochondriahasjustbeen
discovered.Whichofthefollowingwouldthisorganismmostlikelypossess?
a) Lysosomes
b) Cilia
c) Ribosomes
d) Endoplasmicreticulum
40. Matchwithmostappropriateone.

A.
B.
C.
D.
E.
F.

TFIIA
TFIIB
TFIID
TFIIE
TFIIF
TFIIH

i)InteractswiththeTBPandaidsitsbindingwithTATAbox
ii)MakessequencespecificproteinDNAinteractionsandformsacomplex
iii)Bindstothecorepromotertopositionthepolymeraseproperly
iv)InvolvedinDNAmeltingatthepromoterandbindtosinglestrandedDNA
v)StabilizestheRNApolymeraseII
vi)Consistsoftensubunits,havehelicaseandATPaseactivities.

a) Ai,Bii,Ciii,Div,Ev,Fvi
b) Aii,Bi,Ciii,Div,Ev,Fvi
c) Aii,Bi,Ciii,Dv,Eiv,Fvi
d) Noneofaboveiscorrect
41. Matchthefollowing

A. IF1
i.
EnterProkaryoticsubunitintheAsite
B. IF2
ii.
BindstoFormyltRNAandcontrolstheentry.
C. IF3
iii.
Enables30SsubunitstobindtomRNA
D. eIF1
iv.
EssentialfortransferoftRNAmetto40SsubunittoformPreinitiationComplex
E. eIF2
v.
Formationofaternarycomplex(TC)withGTPandtRNAmet
F. eIF3
vi.
Preventingthelargeribosomalsubunitfromprematurelybinding.
G. eIF4A vii.
HelicaseofRNA
H. eIF4B viii.
Enhancestheprocessivityofhelicase
I. eIF4E
ix.
Bindsto7methylGuaninecap
J. eIF4G
x.
BindstoPolyAtailthroughPABP

a) Ai,Bii,Ciii,Dv,Eiv,Fvi,Gviii,Hvii,Iix,Jx
b) Ai,Bii,Ciii,Div,Ev,Fviii,Gvii,Hvi,Iix,Jx
c) Ai,Bii,Ciii,Div,Ev,Fvi,Gvii,Hviii,Iix,Jx
d) Noneofaboveiscorrect

42. AllofthefollowingstatementsaretrueaboutdamagebyultravioletlighttoDNAinlivingcells
EXCEPT:
a) ThedamageblocksnormalDNAreplication.
b) Themostdamagingwavelengthisabout260nm.
c) Covalentbondsareformedthatjoinneighboringpyrimidines.
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43.

44.

45.

46.

47.

48.

49.

50.

d) Neighboringphosphodiesterbondsarecleaved.
Geneswithinanoperon:
a) Tendtoberegulatedbyacommonregulatorymechanism.
b) Aregenerallyinvolvedinthesamebiochemicalpathway.
c) AreexpressedasapolycistronicRNA.
d) Thisisthecorrectanswer.

e) Alloftheabove.
Atranslationalregulatoryprotein:
a) BindstoDNAandpreventstranslation.
b) BindstomRNAandpreventstranslation.
c) BindstorRNAandpreventstranslation.
d) BindstotRNAandpreventstranslation.
Theoperatorregionnormallycanbeboundby:
a) Attenuator.
b) Inducer.
c) mRNA.
d) Repressor.
Theprimereasonofe.colicannotutilizelactosebecause
a) Absenceofbetagalactoseenzyme
b) Absenceoflactosepermease
c) both
d) None
ThetryptophanoperonofE.coliisrepressedbytryptophanaddedtothegrowthmedium.The
tryptophanrepressorprobably:
a) BindstoRNApolymerasewhentryptophanispresent.
b) Bindstothetrpoperatorintheabsenceoftryptophan.
c) Bindstothetrpoperatorinthepresenceoftryptophan.
d) Bindstotherepressorinthepresenceoftryptophan
WhichstatementisNOTcorrectaboutthelacoperon?
a) Itregulatestheproductionofaseriesoffiveenzymes.
b) Itisnormallyturnedoffifglucoseispresent.
c) Lactosebindstotherepressorproteinandinactivatesit.
d) Itisaninduciblesystem.
The______stimulate(s)apoptosis.
a) p53gene
b) BRCA1andBRCA2genes
c) rasoncogene
d) bcl2protein
Eukaryotic cells with DNA damage often cease progression through the cell cycle until the
damageisrepaired.Thistypeofcontroloverthecellcycleisreferredtoas
a) Damagecontrol
b) Checkpointcontrol
c) Anticyclincontrol
d) Transcriptionalcontrol

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Answer to the M.Sc. entrance class Chapter objective Test 10

Marks:

Name:
_____________________________________
_____________________________________

DARKEN THE CIRCLE FOR CORRECT RESPONSE. RESPONSES ONCE GIVEN CANT BE CHANGED.

Q. No.

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