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SHRIMP
Welcome to Expert Topic. Each issue will take an in-depth look
at a particular species and how its feed is managed.
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2
1
The evolution
of modern
shrimp farming
by Malachi Stone, International
Aquafeed
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by Tilman Wilke, Susanne Kirwan (Dr. Eckel GmbH, Niederzissen, Germany), and Niti Chuchird,
Hataitip Niyamosatha (Aquaculture Business Research Center, Kasetsart University, Bangkok, Thailand)
Dr Eckel was one of the first European feed additive producers who
expanded its business to aquaculture and aquafeed industries. The success stories in livestock feed was encouragement to transfer the natural
nutraceutical concepts to aquafeed applications. Hence, the target was
to develop a functional feed additive that has positive effects on growth
and immune defence of Pacific White Shrimp.
After two years of research and development Dr Eckel is proud to
reap the fruits of its labours. In 2014, the Dr Eckel research partners
of the Aquaculture Business Research Centre at Kasetsart University
in Bangkok reported a breakthrough. The tested phytogenic feed additives proved to have positive effects on growth, survival and immune
response of shrimp.
In a series of tank trial experiments at Kasetsart University the
effects of dietary supplementation of phytogenic feed additives was
Survival rates did not differ between groups in the growth experiments. However, in the challenge experiment shrimp from the group
fed with Dr Eckel feed additives at 800 ppm and at 400 ppm had
significantly higher survival rates (78 percent and 67 percent respectively) compared to shrimp in the control group (64 percent) when
challenged with a virulent strain of Vibrio parahaemolyticus.
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cal effects as one of the target mechanism of the novel feed additive.
The tested feed additive improved shrimp immune response, which
led to higher survival rates in the challenge experiment. In the course
of their immunological studies the researchers measured the ratio
of hemocytes cells that do phagocytosis to the total number of
hemocytes. Phagocytosis is the central and terminal mechanism of the
BTR
immune system to seek
and destroy pathogenic bacteria or infected
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cells. The share of hemocytes that perform phagocytosis was significantly larger in the treatment group (23 percent) than in the control
group (17 percent). Additionally shrimp in the treatment group had
twice as much hemocytes compared to shrimp of the control group
(4.4 x 106 versus 1.9 x 106 cells / ml).
When the researchers examined the hepatopancreas of the challenged animals they discovered a spectacular histopathological pattern:
Shrimp from the treatment group had less cell necrosis in the hepatopancreas compared to other groups (Figure 2). That means that the
phytogenic feed additive from Dr. Eckel took a cell-protective effect
in the hepatopancreas of shrimp.
Ge
Leiber GmbH
Hafenstrae 24
49565 Bramsche
Germany
Tel. +49 (0)5461 9303-0
Fax +49 (0)5461 9303-29
www.leibergmbh.de
info@leibergmbh.de
2
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Control
MFeed+ 0.1
percent
Variation over
control
MFeed+ 0.2
percent
Variation over
control
6.32 0.13
6.32 0.13
6.32 0.13
12.4 0.8
13.09 1.1
+5.6%
13.44 0.1
+8.4%
1.22 0.1
1.31 0.1
+7.4%
1.34 0.1
+9.8%
8.66 0.64
8.21 0.92
8.52 0.77
1.42 0.1
1.22* 0.09
-14.1%
1.23* 0.1
-13.4 percent
* p-value < 0.05
Control
MFeed+ 0.1
percent
Variation over
control
MFeed+ 0.2
percent
Variation over
control
3.07 0.39
2.83 0.31
-7.8%
1.17** 0.13
-61.9%
1.67 0.30
1.58 0.25
-5.4%
1.13 0.12
-32.3%
67.2
4.38
78.4**
4.56
+16.7%
82.4** 3.58
+22.6%
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increased, hence a better digestibility of feed
and increased nutrient uptake.
Nevertheless, it seems that the action
of clays to enhance feed digestion in the
intestine also involves other mechanisms.
Reichardt (2008) and Habold et al (2009)
both report the ability of clays to favor the
contact between enzymes and nutrients and
therefore to improve the rate of digestion
of the feed. Indeed, digestive enzymes need
to be in contact with their substrate in order
for hydrolysis to occur. The physico-chemical
interactions of the enzymes with clay particles
seem to enhance the contact between the
digestive enzymes and the feed, making clays
a good supporting matrix for enzymes and
acting as a meeting point for them to be in
contact with their substrate. Indeed, Cabezas
et al (1991) demonstrated that clay-enzyme
complexes are formed at enteric pH values.
These active stable complexes are resistant
to proteolysis and increase the amount of
active digestive enzymes in the intestine, thus
improving nutrient digestibility.
In the same way, Habold et al (2009)
observed higher pancreatic lipase activity in
rats supplemented with Kaolinite; Xia et al
(2004) showed an increase in small intestinal
digestive enzyme activity in broilers supplemented with Montmorillonite; and Paolo et
al (1999) observed an increase in protein and
energy retention coefficients for growing pigs
et al, 2009). Cofactors are defined as thermostable non-protein compounds that form the
active portion of an enzyme system. In other
words, cofactors are helper molecules required
for enzymes to be active. They can be organic
www.catalysisagrovet.com
January-February 2015 | INTERNATIONAL AQUAFEED | 41
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or inorganic, most commonly vitamins in the
first case and metallic ions in the latter.
Clays are layered mineral materials, composed of a succession of aluminum and
silicium based sheets, the order of which
varies depending on the type of clay. In
Montmorillonite, several metallic ions replace
some aluminum and silicium ions in the structure. Known as the substitution phenomenon, this event provides montmorillonite
with part of its physico-chemical reactivity. Moreover, the presence of metallic ions
may contribute to the activation of some
enzymes, through their action of cofactors
(Niederhoffer, 2000). In this way, copper
is known to activate lipase and phospholipase A (Jondreville et al, 2002) and zinc
is a required cofactor of carboxypetidase
(Williams, 1960), to mention only a couple
of examples.
The combination of the matrix support
provided by the clay and the cofactor effect
coming from the metallic ions present in its
structure can be referred to as biocatalysis:
the improvement of performance of a biochemical reaction through the action of an
external compound, a biocatalyst. Due to a
large variety of clay minerals, one can imagine
that all clays do not have the same potential
for biocatalysis depending on their type, their
purity, their source or their treatment. As
such, clay structure can be modified and asso-