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etary intake variables (current vitamin D supplementation and daily cows milk intake) on 25-hydroxyvitamin
D level in early childhood and to evaluate the relationship between these modifiable dietary factors and other
largely nonmodifiable determinants of vitamin D status
including skin pigmentation and season.
Design: Cross-sectional study.
Setting: Primary care pediatric and family medicine practices participating in the TARGet Kids! practice-based research network in Toronto, Ontario, Canada.
Participants: From December 2008 to June 2011,
healthy children 1 to 5 years of age were recruited during a routine physicians visit.
Interventions: Survey, anthropometric measurements, and laboratory data were collected. A multivariable linear regression model was developed to examine
the independent effects of vitamin D supplementation and
daily volume of cows milk on 25-hydroxyvitamin D level.
tamin D deficiency causes rickets but less severe vitamin D deficiency (vitamin D levels of
10-30 ng/mL) is emerging as a
risk factor for a number of acute and
chronic illnesses including asthma and allergies in children.1-12 Understanding modifiable determinants of vitamin D status in
early childhood is of practical importance to dietitians, physicians, and parents of young children.13-15
It has been suggested that dietary intake of vitamin D is an important modifiable determinant of vitamin D status in
young children.16-19 Further, a number of
studies have identified that the major dietary sources of vitamin D in young children are vitamin Dfortified cows milk and
vitamin Dcontaining supplements.20-26
JAMA PEDIATR/ VOL 167 (NO. 3), MAR 2013
230
Yet, the effect of cows milk intake and vitamin D supplementation on vitamin D
status is unclear, particularly in the context of known nonmodifiable vitamin D
determinants such as skin pigmentation
and season.
In 2008, the American Academy of Pediatrics Committee on Nutrition recommended that all children older than 1 year
receiving less than 1 L of cows milk per
day be supplemented with 400 IU of vitamin D daily.15 For the first time, healthy
children older than 1 year were recommended to receive supplemental vitamin
D, providing a unique opportunity to study
the effect of vitamin D supplementation
and cows milk intake on the vitamin D status of young children.
The primary objective of this study was
to determine the effect of current vitamin
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Author Aff
Applied He
of the Li Ka
Institute (D
Mamdani a
Departmen
Maguire), S
Paediatric O
Team, Divis
Medicine, T
Children (D
Khovratovi
Mss DeGro
Faculty of M
Departmen
Maguire, Bi
Dalla Lana
Health (Mr
Dan Facult
Mamdani),
Toronto, To
Canada.
Group Info
TARGetKid
Clinical Sit
listed at the
D supplementation and daily cows milk intake on 25hydroxyvitamin D level in a large cohort of healthy urban preschool children. Our secondary objective was to
evaluate the relationship between these modifiable dietary factors and other nonmodifiable determinants of
vitamin D status including skin pigmentation and season.
METHODS
SUBJECT RECRUITMENT
AND DATA COLLECTION
Study participants were recruited by research personnel embedded in 7 participating pediatric and family medicine practices. Data were collected prospectively through a standardized parent-completed survey instrument based on the Canadian
Community Health Survey27; anthropometric measurements including height and weight obtained by trained research assistants; and venous blood sampling collected at the primary care
clinic. Medidata RAVE (Medidata Solutions Inc, http://www
.mdsol.com/) was used as the secure electronic data capture system and data repository for all TARGet Kids! data.
The primary outcome for this study was 25-hydroxyvitamin D level. Specimens were sent daily to the Clinical Biochemistry Laboratory at Mount Sinai Hospital in Toronto. Total
25-hydroxyvitamin D level was measured from sera samples
using a competitive 2-step chemiluminescence assay with a DiaSorin LIAISON 25-hydroxyvitamin D TOTAL.28 Extensive testing and validation of this machine has been performed and has
demonstrated an intra-assay imprecision of 7.2% at a concentration of 85 ng/mL (to convert to nanomoles per liter, multiply by 2.496) and an interassay imprecision of 4.9% at 13 ng/
mL, 8.9% at 31 ng/mL, and 17.4% at 85 ng/mL, values that are
well within acceptable limits for biochemical measurements.29,30
The primary predictor variables were vitamin D supplementation and daily volume of cows milk intake. These were
determined from the response to the questions Does your child
take a vitamin Dcontaining supplement regularly? and How
many 250-mL cups of cows milk does your child drink in a
typical day?
Covariates that might influence vitamin D status were identified through a literature review. Covariates were chosen for
inclusion if they were associated with 25-hydroxyvitamin D in
1 or more published studies and included age, sex, season (MaySeptember vs October-April), daily outdoor play time, daily
screen time, and skin pigmentation. Weight was measured using
a precision digital scale ("0.025%; SECA) and standing height
was measured using a stadiometer (SECA). Body mass index
was calculated as weight in kilograms divided by height in me-
7346 Eligible
1884 Declined
2066 Deferred
STATISTICAL ANALYSES
Descriptive statistics were performed for the main outcome, predictors, and covariates. For our primary analysis, a multivariable linear regression model was developed to examine the independent effects of vitamin D supplementation and daily
volume of cows milk on 25-hydroxyvitamin D level with adjustment of prespecified, clinically relevant covariates (described earlier). All covariates were included in the final model
irrespective of their associated P values.
To reduce the likelihood of overfitting and obtain more conservative estimates of model performance, bootstrap validation of the linear regression model was performed. Additionally, the bootstrap assessment was repeated 100 times to obtain
95% confidence intervals.34 Missing data were handled by single
imputation using conditional means and recursive partitioning.35 A sensitivity analysis including or not including imputed data was also conducted.
For our secondary analyses, we explored the relationship
between vitamin D supplementation and milk intake on
25-hydroxyvitamin D level in the context of childrens skin
pigmentation and season. To accomplish this, we built on the
multivariable linear regression model developed for the primary analyses through the addition of biologically plausible
interactions. These included vitamin D supplementation by
milk intake, vitamin D supplementation by season, vitamin D
supplementation by skin pigmentation, and skin pigmentation by season. To achieve a balance between overfitting and
interpretation and to limit the biases that can result from standard variable selection approaches, these interactions were
tested together. If the joint P value was large (#.30), no further testing was considered. Otherwise, the interactions were
retained in the model. To assess the contribution of each variable and interaction on the variation in 25-hydroxyvitamin D
level, the partial R2 for each was calculated and displayed
graphically.
Data were analyzed using the R project for statistical computing.36 This study was approved by the research ethics board
at The Hospital for Sick Children, and all parents of participating children consented to participation in the study.
RESULTS
STUDY POPULATION
Of the 3396 children who consented to participate, venous blood sampling was obtained in 1898 children
who were included in this analysis (Figure 1). Sev-
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50
% of Total Population
40
Daily vitamin D
supplementation (yes vs no)
Cows milk intake (per cup/d)
Season (May-Sep vs Oct-Apr)
Outdoor free play (per 1 h/d)
Skin pigmentation
(Fitzpatrick type I-III vs IV-VI)
Age (per year)
BMI z score (per unit)
Screen time (per 1 h/d)
30
20
10
25-Hydroxyvitamin D
Effect, ng/mL (95% CI)
Variable
20
40
60
80
100
120
25-Hydroxyvitamin D, ng/mL
Subjects
Age, mo, mean (SD)
Male
BMI z score, mean (SD)
Overweight
Obese
Current breast feeding
Daily milk intake, mL, mean (SD)
Current bottle use
Daily vitamin D supplementation
Daily outdoor play time, min, mean (SD)
Daily screen time, min, mean (SD)
Season (Oct-Apr)
Skin pigmentation (Fitzpatrick type)
1 (lightest)
2
3
4
5
6 (darkest)
37 (18)
964 (51)
0.22 (1.1)
271 (15)
86 (5)
218 (12)
455 (307)
465 (26)
1021 (57)
61 (56)
79 (77)
991 (52)
155 (9)
783 (44)
566 (32)
201 (11)
41 (2)
23 (2)
enty-six percent of children had complete survey, anthropometric, and laboratory data. Fifty-seven percent
of the population were regularly consuming a vitamin
Dcontaining supplement. Mean daily cows milk intake was 455 mL. Mean 25-hydroxyvitamin D level was
35 ng/mL (95% CI, 34.86-35.66 ng/mL). Thirty-five
percent (95% CI, 33%-38%) had a 25-hydroxyvitamin
D level less than 30 ng/mL and 6% (95% CI, 5%-7%)
had a 25-hydroxyvitamin D level less than 20 ng/mL
(Figure 2). Subject characteristics are presented in
Table 1. Imputation for missing values did not change
descriptive characteristics.
EFFECT OF VITAMIN D SUPPLEMENTATION
AND COWS MILK INTAKE
ON 25-HYDROXYVITAMIN D LEVEL
For our primary analysis, results of the multivariable linear regression model are shown in Table 2. In the ad-
P Value
3.4 (2 to 4)
$.001
1.6 (1 to 2)
1.6 (0.4 to 2.8)
0.3 (0 to 0.4)
2.7 (1.2 to 4.4)
$.001
.004
.03
.001
%0.2 (%0.4 to 0)
%0.6 (%1.2 to 0)
0.2 (0 to 0.4)
.05
.03
.22
In this prospectively designed study, we have examined the contribution of 2 modifiable dietary determinants on 25-hydroxyvitamin D level in the context of
known, and largely nonmodifiable, factors including
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No supplementation
Vitamin D supplementation
40
40
40
35
Vitamin D
Vitamin D
35
30
25
30
25
12
3
Mi
nth
nta
lk I
Mo
up
e, C
2
0
nta
lk I
Mi
No supplementation
Vitamin D supplementation
40
up
e, C
30
nth
10
Mo
35
12
10
40
25
35
Vitamin D
35
Vitamin D
45
30
25
30
25
12
10
Mo
nth
2
0
Mil
ups
e, C
k
Inta
12
10
Mo
nth
20
2
0
Mil
ups
e, C
k
Inta
Figure 3. The effect of milk intake, skin pigmentation, vitamin D supplementation, and month on 25-hydroxyvitamin D serum levels. Light skin pigmentation
represents Fitzpatrick skin pigmentation types I to III and dark skin pigmentation represents Fitzpatrick skin pigmentation types IV to VI. Effects adjusted for the
average child (age, 37 months; body mass index z score = 0.2; 1 hour of outdoor play; and 1 hour of daily screen time). To convert 25-hydroxyvitamin D to
nanomoles per liter, multiply by 2.496.
and maintaining optimal vitamin D levels in early childhood may be relevant to health outcomes in later childhood and adulthood.15,37 Dietary records from Canadian infants have demonstrated that at 12 months of
age, average daily intake of vitamin D from complementary food accounts for only 11% of the currently recommended dietary allowance of vitamin D.38,39 Therefore,
other strategies to maintain 25-hydroxyvitamin D level
as children transition from vitamin Dfortified formula or breast milk with vitamin D supplementation
are necessary. To date, there has been limited understanding of modifiable influences on 25-hydroxyvitamin D level in early childhood. Our findings have practical implications for both clinical practice and health
policy.
Our finding that variables reflecting cutaneous production of 25-hydroxyvitamin D (skin pigmentation,
season, and outdoor play time) appear to explain less
variation in 25-hydroxyvitamin D level than dietary
intake variables in early childhood is consistent with
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Milk intake
Supplementation
Skin pigmentation
Season
Outdoor play
BMI z score
Screen time
Age
0.000
0.005
0.010
0.015
0.020
0.025
Partial R 2
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Thompson, BSc, for administrative and technical support for the TARGetKids! program.
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