ANIMAL CARE AND USE STATEMENT

(Protocol and Review Form)

I.

PROCEDURE(S) OR TITLE OF RESEARCH STUDY:

Pro-megakaryopoietic
Thrombocytopenia

II.

effects

of

Quail

Eggs

in

Rats

with

Induced

PURPOSE/ OBJECTIVES:
This study aims to investigate the pro-megakaryopoietic effect of quail eggs in
rats with induced thrombocytopenia. Specifically to determine:
a. The effectiveness of quail eggs in increasing platelet levels.
b. Whether greater consumption of quail eggs leads to higher platelet values.

III.

DURATION OR TIME FRAME:

The research study will be conducted on February up to March, 2015.

IV.

RESPONSIBLE PERSON OR PRINCIPAL INVESTIGATOR:

a. NAME: MARJI SIM JAMIAS-RAGANIT
b. QUALIFICATION (degree or qualification/s): REGISTERED MEDICAL
TECHNOLOGIST, MSMT

V.

BACKGROUND AND SIGNIFICANCE OF THE PROCEDURE OR RESEARCH:

This study aims to investigate the pro-megakaryopoietic effect of quail eggs in
rats with induced thrombocytopenia.

VI.

DESCRIPTION OF METHODOLOGIES/ EXPERIMENTAL DESIGN:

Quail eggs will be administered to Group C rats with induced
thrombocytopenia through oral gavage. The rats to be used are female albino
weighing approximately 100 to 150 grams. Fifteen rats will be needed in the research
study. The rats will be divided into three groups consisting five rats each and will be
placed in a cage. Group A will be the standard, group B will be the negative control,
and group C will be the treatment group. Food and drink will be given to the rats as
diet for the one week acclimatization at the SLU Animal House. Hydroxyurea will be
orally administered to Group B and group C to induce thrombocytopenia.
Administration of quail eggs to group C only will be done for fourteen days.
Dissection and smear preparation of spleen of all the rats will be performed by a
licensed veterinarian at Benguet State University. A pathologist will be reading the
results of the smears at Saint Louis University Laboratory.

VII.

DECLARATION BY THE RESPONSIBLE PERSON:

I ACCEPT RESPONSIBILITY FOR ASSURING THAT THE PROCEDURE/
STUDY WILL BE IN ACCORDANCE WITH THE APPROVED PROTOCOL. I
ASSURE THAT ALL PERSON WHO USE THIS PROTOCOL AND WORK
WITH ANIMALS HAS RECEIVED APPROPRIATE
TRAINING/INSTRUCTIONS IN PROCEDURAL AND HANDLING
TECHNIQUES, AND ON ANIMAL WELFARE CONSIDERATIONS.
I AGREE TO OBTAIN WRITTEN APPROVAL FROM THE INSTITUTIONAL
ANIMAL CARE AND USE COMMITTEE PRIOR TO MAKING ANY
CHANGES AFFECTING MY PROTOCOL. I ALSO AGREE PROMPTLY
NOTIFY THE IACUC IN WRITING OF ANY EMERGENT PROBLEMS THAT
MAY ARISE IN THE COURSE OF THIS STUDY INCLUDING THE
OCCURRENCE OF ADVERSE SIDE EFFECTS.

Signature of the Responsible Person:

_________________________

Date:___________________

Noted:

RUTH C. DIEGO
Chair, BSU-IACUC

Date:___________________

APPLICATION FOR AUTHORIZATION

(For the Conduct of Scientific Procedures using Animals)

1. Name of Entity: Pro-megakaryopoietic effects of Quail Eggs in Rats with Induced

Thrombocytopenia
2. Address: Saint Louis University, Baguio City
3. Telephone number: 444-8246
4. Name and Position of Representative Person:
RAGANIT
Last Name

MARJI SIM
First Name

JAMIAS
Middle Name

Position: RESEARCH PROMOTER

5. Description/ Profile of entity (attach organization chart):
This study is A Research Proposal in Partial Fulfillment of the Requirements for the
Degree, Bachelor in Medical Laboratory Science.
6. Purpose of the conduct of Scientific Procedures (encircle one or more):
a. Biochemical research experiment, studies, investigation (including pre-clinical
research)
b. Teaching and Instruction
c. Product testing
d. Production of antiserum or other biological
7. Identify the key Instructional representatives (including the ACLUC Chairperson,
Veterinarians, and researcher)
I certify that the statements made herein are correct and true:

______________________

__________________________

Signature of Representative

Signature of Head of Institutions

Date:__________________

Date: _____________________