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Source:
http://mycourses.mycandi.ac.uk/pluginfile.php/237165
(Core practical)
/mod_resource/content/0/01_core_practicals/Activity_
2.11_Enzyme_concentration/Notes%20for%20writing
%20up%20Enzyme%20core%20practical%20_v1.pdf
Maryam Jamshidi
(Core practical)
Maryam Jamshidi
(Core practical)
time to 50%T / s
Enzyme
conc. / gdm3
1
2
3
4
5
1
1
8
1
2
6
4.
5
3.
5
2
23
13.
3
12.
2
9.1
8.7
8
12 /
4.
2 /
4. 10.
1
4
3.
5 5.3
3.
2 6.5
5
17.
9
13.
2
8.8
1
7.7
6
6.0
6
6
15
7
1
3
8
1
7
1
3
1.5
3
2.5
7
9
24.3
5
18.7
6
14.8
8
10.1
7
5.9
mea
n
S
D
18
11
mea
n
0.05
7
0.09
1
0.12
1
0.16
0
0.21
2
SD
0.014
3
0.040
3
0.064
1
0.062
8
0.106
5
Maryam Jamshidi
(Core practical)
0.200
0.150
rate of reaction/s-1
0.100
0.050
0.000
0.5
1.5
2.5
3.5
4.5
5.5
Through my class results I can see that as less water is used to dilute the
trypsin solution (so the stronger the concentration of the trypsin) the less
time it takes for the trypsin to break down the casein molecules in the
milk mixture added to the enzyme solution.
Interpretation:
It seems that the higher the trypsin concentration, will mean that there is more
trypsin enzymes within the given volume, which will allow the casein to hit an
active site of an enzyme more likely than a diluted solution as there are more
trypsin molecules available compared to water molecules.
Evaluation:
According to the standard deviation, there appears to be a very wide range in
the time taken for the trypsin to react for each individual group. This makes the
data unreliable as the standard deviation shows that there are likely to be
anomalies in the results. Accuracy was not able to be measured properly as the
Maryam Jamshidi
(Core practical)
experiment wasnt repeated, instead, as a class it was done once by each group,
and results were put together to show a range of the results. People tried to be
very accurate, to make a fair class result, but although precision was said to be
carried out, it cannot be proved and therefore not is not very reliable.
The syringe doesnt very accurately measure the volumes of water and trypsin
required and also can gather bubbles within, which will affect the overall volume
of the solution.
The stopwatch may have not been too accurate as time is wasted to start and
stop the clock, which has an impact on the rate of reaction that we write down.