Beruflich Dokumente
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his revised key for the identification of bacterial pathogens of fish was developed by Dr. Emmett
Shotts in 2001 and was designed to replace and simplify the key found in the 1994 edition of the
Blue Book. This new key provides a broad perspective of strategies useful for the biochemical
identification of bacterial fish pathogens and it will be helpful in both diagnostic work and in teaching.
Users of these new tables must keep in mind that this is a presumptive key and thus it may lead to an
incorrect identification of bacterial isolates having atypical biochemical characteristics or of species not
included in this key. It is also important to note that some bacteria require special growth media or
conditions for growth and that the initial isolations must be made using appropriate substrates and
conditions. Diagnosticians should use this key as a helpful guide, but final diagnostic decisions should
be based on more information provided in other specific bacteriology subchapters of this chapter or in
Section 1, 1.1 General Procedures for Bacteriology.
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2014
Yellow-Pigmented Colonies
Cell Morphology
Cocci
Rods
No
See Figure 2
Gram + Cocci
Gram + Rods
Reaction
Positive
Yes
See Figure 6
Yellow Pigmented
Rods
Negative
See Figure 4
See Figure 5
Aeromonas,
Pseudomonas,
and Vibrio
Gram Negative,
Oxidase Negative,
Rod-shaped bacteria
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LAP Testa
No
Hemolysis on
Blood Agar
Micrococcus Staphylococcus
Aerococcus
alpha (green)
beta (clear)
gamma (none)
P Disc +
Inhibitionb
- Bile +
Solubility
Lancefield
typeable
CAMP
test
Streptococcus
viridans
Group
Streptococcus
pneumoniae
Group
No Yes
Streptococcus
Other
Miscellaneous Nonhemolytic
iniae
Streptococcus Streptococcus Streptococcus
suspect
Lancefield Group B
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Yes
Acid Fast
Aerobe or
Facultative
Anaerobe
Anaerobe
Yes
No
Mycelia
Bacillus
Special
Media
Required
Clostridium
Yes
Nocardia
No
Mycobacterium
No
H2S
Production
Yes
Renibacterium
No
Esculin Hydrolysis
Erysipelothrix
or
Yes
No
Hemolysis
Carnobacterium
Lactobacillus
Yes
Listeria
( hemolysis
on blood agar)
(none)
Corynebacterium
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+
O/129 Sensitivity + Novobiocin Sensitivity -
Yes
Inositol
Yes
Miscellaneous
Nonfermenters
No
Motility
Pseudomonas
fluorescens
Group
Plesiomonas
Growth
at 35C
No
No
Yes
Yes
Yes
Pseudomonas
putida
Group
Aeromonas salmonicida
Group
Motile
Aeromonas
Group
Pigment
+
< 2 mm Colony Size > 2 mm
> 5 days Growth Rate < 5 days
Atypical
Typical
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No
+ Indole - Citrate +
Phenylamine
Deaminase
No
Escherichia
Yes
Motility
H2S
No
Enterobacter
Yes
Klebsiella
Yes
Ornithine Proteus
Decarboxylase
Citrate
No
No
+ H2S - DNAse +
Yes
Providencia Morganella
Motility at 35C
Serratia
Salmonella
Indole
No
- ONPG +
+ Maltose Malonate
Edwardsiella
ictaluri
Yes
No
Yes
Edwardsiella
tarda (H2S+)
Hafnia
Edwardsiella
hoshinae
- Lysine +
+ Arginine - Ornithine +
Yersinia
intermedia
Yersinia
ruckeri
3 % NaCl
Required For Growth
Yellow
Flavobacterium
branchiophilum
(Gelatinase Positive)
Yes
No
Rhizoid Colony
Morphology
Tenacibaculum
maritimum
No
Colony Tightly
Adherent
To Agar
No
Yes
Flavobacterium
columnare
Yes
Flavobacterium psychrophilum
1
Updated