Protocolo Precellys

Precellys Application Database_Lysis protocols_Human & animal cells & tissues_v0413e
Successfully tested grinding parameters for
human and animal cells and tissues

Human and animal cells and tissues
Precellys parameters
(number of cycles, time period, rpm, pause)
Starting material
(amount)
Target molecule
(yield)
Buffer conditions/remarks
Bat (Pteropus vampyrus): spleen

in RNAlater
RNA
RNeasy Kit
CK14
2 x 20 s @ 6800 rpm
Beaver: muscle
(40 mg)
DNA
400 l Tissue DNA lysis buffer
CKM
1 x 15 s @ 6500 rpm
Beetle
(20, 80, 200)
RNA
0,8; 1,5; 4,5 ml TRIzol, partly with

Cryolys, good homogenisation
7 ml + 2 x
SK38 filling
5/15 s @ 6500 rpm
Beetle
PCS-Kit
Good homogenisation
CKM
2 x 20 s @ 6000 rpm; 10 s
pause
Bovine: muscle
(20 mg)
DNA
(approx. 10 g)
200 l Lysis B. T + 20 l Prot K

peqGOLD Tissue DNA-Mini-Kit
CK14
1 x 5 s @ 5000 rpm
Bovine: brain
RNA
TriFast; good homogenisation
CK14
2 x 10 s @ 5500 rpm
5 s pause
Bovine: colon, stored in RNAlater RNA

"rubber-like" (approx. 100 mg)
1 ml peqGOLD TriFast
MK28
3 x 60 s @ 6800 rpm,
30 s pause
Bovine: kidney, lung
RNA
CK14
1 x 20 s @ 5000 rpm
Bovine: muscle
RNA
CK28L
2 x 10 s @ 5500 rpm
5 s pause
Bovine: rumen
(50 mg)
RNA
500 l RLT buffer
CK28
2 x 20 s @ 6200 rpm
30 s pause
Bovine: muscle
(100 mg)
Protein
1 mL TES buffer (10 mM Tris (pH 7.6),

1 mM EDTA, 0.25 M sucrose)
Ck28
2 x 20 s @ 6000 rpm
Bovine: brain
(50 mg)
Prion protein
500 l 5 % glucose sol.
CK14
2 x 30 s @ 6500 rpm
Bovine: probably muscle
Metabolites
350 L methanol (d 4 ) and 150 L

0.2 M sodium phosphate buffer (pH 7);
1 H-NMR spectra analysis to identify
the geographical origin of the animal
CK28
2 x 20 s @ 5000 rpm
Bovine: muscle
(200 mg)
Metabolites
200 oder 500 l methanol-containing

buffer
CK14S; CKM
Bug (Naucoris cimicoides)

(20 mg larvae)
DNA
300l lysis buffer (15% Sucrose, 0.05

M Tris pH 8.5 ,0.05 M EDTA) + lysozyme
200 l 0,1
ceramic
beads
Caenorhabditis elegans
RNA/DNA
Different lysis buffer
RNA with parts of

gen. DNA
500 l M9-buffer, Qiagen RNA extraction, successful RT-PCR
VK05
2 x 10 s @ 6500 rpm
RNA
TRIzol; RNA quality very good, Bioanalyzer RIN > 9.7; 28S/18S > 2
VK05
2 x 5 s @ 6500 rpm; 10 s
CK14
2 x 20 s @ 6500 rpm
30 s pause
(suspension of an entirely covered 9 cm Petri dish)
(15 mg, lyophilised)
(20 mg, lyophilised)
Caenorhabditis elegans, fresh

and frozen
Protein
(high yield & spots on
2D gel)
Protein
Protein
500 ml 6 M Urea, 2 M Thiourea, 4 %

CHAPS, 6.5 mM DTT, 1 % Prot K
Inhibitor Cocktail, 3 % Servalyte 3-10
750 l urea-containing lysis buffer;
sample must not be warmer than 40C;
in experiment 32C was obtained
Good results in Western blot, comparable to homogenisation using a vortex
VK05; CK14
CK14; SK38
CK14
1 x 20 s @ 6000 rpm, mit

Cryolys cooling (level 1)
3 x 30 s @ 6800 rpm
1 2 x 20 s @ 5400 rpm; 10
s pause
2 x 20 s @ 6500 rpm; 30 s
pause
2 x 20 s @ 6500 rpm
info@peqlab.com - www.peqlab.com

Starting material
(amount)
Target molecule
(yield)
Cat: spleen, liver
Bacterial DNA
200 l PBS
CK14
1 x 30 s @ 6800 rpm
200 L of a wasser/acetonitrile (1:1)

mixture
CK14
2 x 30 s @ 6000 rpm
1 ml PBS
VK05
3 x 10 s @ 6500 rpm
15 s pause
Cell culture: Hepatocytes (human) RNA, pharmaceut.

cell pellets
substances
PCS-Kit
Cell culture
Protein, native
("plenty of")
Cell culture
Arsen
CK14
1 x 30 s @ 6000 rpm
Cell culture (CH4, Hela, NB231)
Protein
CK14
2 x 30 s @ 5000 rpm
Cell culture: PC12 cells
Protein
RIPA buffer with protease and phosphatase inhibitors, successful protein

analysis
CK14
2 x 30 s @ 5000 rpm
Cell culture: human SW480-line

(7 x 106 cells)
Metabolites; GC-MS
800 l Methanol/H2O (80:20)
CK14
2 x 15 s @ 5500 rpm
Centipede
DNA
CK14
1 x 15 s @ 5000 rpm
Chicken: thigh (Tibia), skeletal

muscle, hyaline cartilage
(100 mg)
RNA
1 ml TriFast; successful RT-PCR
MK28R
8 x 10 s @ 6500 rpm
20 s pause on ice each
Chicken: Bursa fabricii

(50 100 mg)
Protein
1 ml ice-cold 2D-DIGE lysis buffer/100

mg
CK14
CK14s
2 x 10 s @ 5000 rpm
Crayfish: Abdomen, muscle,

eyes, tendons (10 20 mg)
RNA, DNA of a
700 l CTAB-buffer (prewarmed to
pathogenic fungi
65C), successful RT-PCR and PCR
(Aphanomyces astaci)
MK28R
3 x (1 x 60 s @ 6500 rpm);
2 min pause
Dolphin: spleen
RNA
Qiagen extraction kit
CK14
2 x 15 @ 5500 rpm
Drosophila: entire (adults)
Nukleic acids
500 l buffer
(same result as with grinding)
CK14
3 x 30 s @ 6800 rpm
15 s pause
Drosophila: entire (10 adults)
Protein
(810 g)
in 100 l TBS/0.1 % Triton and Protease inhibitor
CK14S
1 x 25 s @ 5500 rpm
Drosphila: entire (4 adults)
Protein
in 150 l buffer; good homogenisation;

MINILYS
CK14S
1 x 25 s @ 5000 rpm
Drosophila: Larven (8)
Lipide
1mL 0.05% Tween-20
CK14/
CK14S
1 x 10 s @ 5000 rpm
Drosophila: larvae (15) or heads

of adults (80)
Lipids
1 ml buffer, GS/MS analysis
CK28
2 x 25 @ 5500 rpm
Fish (eal): kidney and gills

(100-150 mg)
Protein
50 mM Tris, 1 mM MgCl2 (pH 7.4)

buffer with Protease Inhibotor Cocktail,
ice-cold (1:10 w/v)
CK28R
3 x 20 s @ 6500 rpm
Fish (cod): intestinal segments
RNA
1 ml Qiazol lysis buffer
CK14
1 x 30 s @ 6000 rpm
Fish: pufferfish: muscle and liver

in RNAlater at -20C
(100 mg, in 7 x 7 mm pieces)
RNA
1 ml lysis reagent of RNeasy Mini Kit,

good results with MINILYS
CKM
2 x 30 s @ 5000 rpm
15 s pause
Fish (stickleback): liver

(50 150 mg fresh weight)
RNA/metabolites
8 ml/g (v/w) methanol and 2,5 ml

(v/w) water
CK14
2 x 10 s @ 6400 rpm
30 s pause
Fish (salmon): muscle

(300 mg)
DNA/Protein
1 ml extractionbuffer (100 mM Naacetate in 0,2 % Triton X-100, pH 5,5
CK28R
2 x 20 s @ 5500 rpm
Fish (salmon): colon

frozen
RNA
QIAzol lysis buffer
CK28R
2 x 30 s @ 6500 rpm
Fish (salmon): liver

frozen
RNA
QIAzol lysis buffer
CK28R
3 x 10 s @ 6000 rpm

Starting material
(amount)
Target molecule
(yield)
Fish (salmon): all kinds of organs
RNA
1 ml TRIzol
Fish (salmon): muscle

(300 mg)
Enzyme activity
1 mL 100 mM Na-Acetate in 0.2%

Triton X-100, pH 5.5; successful measurement of activity
Fish: gonads
Protein
Fish: liver
Protein
Flies (Heleomyza borealis):

larvae, adult insect
20 mg fresh weight, frozen
PCS-Kit
MK28 empty
+ 2 beads of
MK28
2 x 25 s @ 5000 rpm
CKM
2 x 20 s @ 5000 rpm
10 s pause
CK14
3 x 10 s @ 6000 rpm
Phosphate buffer pH 7.6
CK14
2 x 10 s @ 6000 rpm
Metabolites
200 l ice-cold methanol:water mixture

(2:1)
CK14
3 x 30 s @ 6800 rpm
Flies: larvae
(50 mg)
Drugs
500 l H2O deion., successful isolation

of drugs, use in the forensic field
MK28R
1 x 30 s @ 4000 rpm
Frog: eggs (in RNALater)

(40 mg)
RNA
400 l RNA Lysis B. T-P

(peqGOLD Total RNA Kit) or 1 ml
peqGOLD TriFast
CK14
2 x 20 s @ 5500 rpm,
15 s pause
Frog: tails and eggs
RNA
RLT-buffer
CK14; CK28
1 x 20 s @ 6500 rpm
CK28
2 x 50 s @ 6200 rpm
CK14
CK28
2 x 10 s @ 5000 rpm
CK14
CK28
3 x 5 s @ 6500 rpm
CK14
1 x 30 s @ 6000 rpm
CK28
1 x 40 s @ 5000 rpm
CK01
3 x 30 s @ 6500 rpm
45 s pause
CK28L
1 x 20 s 6500 rpm
CK28
2 x 30 s @ 5900 rpm
15 s pause
CKM;
MK28R
1 x 15 s @ 5500 rpm
Frog: skin
Horse: liver
RNA
Horse: tonsils
RNA
Game animals: liver
Arsen
Game animals: muscle

(approx. 0,5 x 0,5 cm)
Metabolites
Human: biofilm of colon, tissue of DNA of microorgaa biopsy

nism
Human: bone neoplasm
(500 mg)
Lysis buffer
Frozen tissue in methanol/chloroform

sol.; satisfying homogenisation; use of
MINILYS
200 l buffer ATL (Qiagen) + 20 l
proteinase K or 200 l buffer mix (20
mM Tris/HCL pH 8.0, 2 mM EDTA, 1.2
% Triton X-100, lysozyme (20 mg/ml)
DNA?
(good yield)
Human: bones, pre-homogenised

in N2 in Freezer Mill
DNA
(200 mg)
400 ml extraction buffer; in comparison to conventional Prot K method very

good and usable results in the field for
ensic
100 l RNA Lysis buffer T-P; little RNA
yield, not measurable, DNA visible on
gel, no improvement with Cryolys
Human: cartilage of wrist joint

(50 mg)
DNA/RNA
Human: finger nails

(20 mg)
DNA
200 l lysis buffer; yield: 4 g/20 mg

= 2-fold compared to other protocols
MK28
6 x 20 s @ 6500 rpm;
Human: foetal or neonatal tissue

(skin) (0,5 cm2); chorionic villi
DNA
190 l EZ1 lysis buffer (Qiagen); good

yield in comparison to proteinase K
treatment
CK14
3 x 10 s @ 5500 rpm
15 s pause
Human: hair
(150 mg)
DNA/drugs
Dry; 1 H NMR-Spectroscopy
CK28L
2 x 20 s @ 6500 rpm
Human: liver
(20 mg)
DNA/RNA
600 l Prot K-buffer/1 ml TriFast,

MINILYS
CKM
1 x 15 s @ 5000 rpm
Human: lung
DNA
1 ml buffer; yields 10-20 % higher

than with TissueLyser
SK38
2 x 20 s @ 6500 rpm
2 x 30 s @ 6500 rpm

Starting material
(amount)
Target molecule
(yield)
Human: lung
(20 mg)
DNA/RNA
600 l Prot K-buffer/1 ml TriFast,

MINILYS
CKM
2 x 15 s @ 5000 rpm
Human: nasal concha

(30 mg)
DNA/RNA
500 l PBS and 500 l 3M urea,

MINILYS, satisfying homogenate (small
pieces of connective tissue and matrix
remained)
CKM
MK28
3 x 60 @ 5000 rpm
Human: prostate tissue
Nucleic acids
Anti-Foam-reagent von Sigma-Aldrich
CK28
2 x 20 s @ 6000 rpm
15 s pause
Human: prostate tissue
DNA
CK28R
2 x 20 s @ 6000 rpm
Human: salivary stone

(approx. 5 mg)
DNA/RNA
200 l PBS, MINILYS, satisfying homogenate
CKM
MK28
3 x 60 s @ 5000 rpm
Human: black skin cancer

(20 mg)
DNA/RNA
600 l Prot K buffer/1 ml TriFast,

MINILYS
CKM
1 x 25 s @ 5000 rpm
Human: teeth, ground

(100 mg)
DNA
1,2 ml extraction buffer; EZ1 Biorobot,

Qiagen
CK28
2 x 30 s @ 5900 rpm
Human: teeth
DNA
MK28R
3 x 50 s @ 6500 rpm
Human: adipose tissue

(100 400 mg)
RNA
RLT-buffer + -Mercaptoethanol,
higher yield than with Ultra Thurrax
CKM
2 x 15 s @ 6000 rpm
20 s pause
Human: adipose tissue; thyroid

gland
RNA
High quality RNA on gels, Bioanalyzer and photometric measurements
CKM
2 -3 x 10 s @ 5000 rpm
Human: bladder tissue

(approx. 100 mg)
RNA
CK28
2 x 25 s @ 6000 rpm
20 s pause
Human: brain
(450 mg)
RNA
4,5 ml TriFast, satisfying homogenate
CK28L
2 x 15 s @ 5500 rpm
15 s pause
Human: foetal brain

(approx. 60 mg)
RNA
1 ml peqGOLD TriFast; high yield
CK14
2 x 10 s @ 5500 rpm
5 s pause
Human: breast cancer tissue
RNA
CK14
1 x 20 s @ 5500 rpm
Human: cardiac valves, strongly

calcified
(approx. 100 mg)
RNA
(good yield)
600 l RLT buffer;

RNeasy-Mini-Kit, Qiagen
CK28
2 x 20 s @ 6500 rpm
15 s pause
Human: cartilage tumor

(50 100 mg)
RNA
TRIzol reagent (Invitrogen) + 200 l

Guanidine thiocyanate 4M; high
quality RNA
CK28R
3 x 15 s @ 6500 rpm
10 s pause
Human: colon
(30 mg, in RNAlater)
RNA
Good results, 2-fold yield of mRNA

with Cryolys than using only PCS
CKM
1 x 15 s @ 6000 rpm
Human: colon
(30 mg)
RNA
11 g
250 l TRIzol; satisfying results
CK28
2 x 10 s @ 6500 rpm
10 s pause
Human: glioma = brain tumor

(Neuroepithel); (10 15 mg)
RNA
1 ml QIAzol lysis buffer
CK14
2 x 40 s @ 5500 rpm
Human: hair follicle
mRNA
350 l RLT-buffer + -Mercapto-ETOH;

RNeasy Micro kit
MK28R
2 x 50 s @ 6500 rpm
Human: liver, adipose tissue

(55mg /300 mg)
mRNA/Protein
500 l lysis buffer with protease inhibitor
CK14
2 x 15 s @ 6500 rpm
Human: liver
(1/2 = 450 mg)
RNA
CK28L
2 x 15 s @ 5500 rpm
15 s pause
Human: lymph node metastases
RNA
600 l lysis buffer (RTL and Mercaptoethanol)
CK14
1 x 20 s @ 5500 rpm
Human: kidney
(2 x = 500 mg)
RNA
CK28L
2 x 15 s @ 5500 rpm
15 s pause
PCS-Kit

Starting material
(amount)
Target molecule
(yield)
Human: muscle
(50 mg)
RNA, Protein, chem.

substances
CK14
1 x 20 s @ 5000 rpm
Human: nose tissue with mucosa
RNA
CK14
1 x 10 s @ 5500 rpm
Human: oral mucosa tumorsstored in RNAlater
RNA
(high yield)
600 l RLT buffer

(RNeasy-Mini-Kit, Qiagen)
CK28
1 x 25 s @ 6500 rpm
Human: palatine tonsil

(100 mg)
RNA
CK28
1 x 30 s @ 6500 rpm
Human: pancreas
(3 x = 135 mg)
RNA
CKM
2 x 15 s @ 5500 rpm
15 s pause
Human: skeletal muscle
RNA
(high yield)
1 ml peqGOLD TriFast;
CK14
2 x 10 s @ 5500 rpm
5 s pause
Human: skin
(approx. 5 x 10 mm, cut in small
stripes, frozen in N2)
RNA
500 l Trireagent Sigma
CK28
3 x 20 s @ 6200 rpm
15 s pause
Human: skin biopsy
RNA
Lipid TissuE Kit, Agilent Nanochip
CK14
4 x 23 @ 6300 rpm
Human: skin biopsy
RNA
RA1 Lysis buffer (Macherey-Nagel) +

1% -Mercapto-Ethanol
CK14
6 x 23 @ 6300 rpm
Human: skin node, strongly

collagenous
RNA
CK28
3 x 60 s @ 6500 rpm
30 s pause
PCS-Kit
Human: testis
(20 mg)
RNA
500 l RLT buffer
CKM
1 x 5 s @ 6000 rpm
Human: tumor tissue (Meningioma) (10 15 mg)
RNA
1 ml QIAzol lysis buffer
CK14
2 x 40 s @ 5500 rpm
Human: bladder tumor

(500 mg)
Protein
2 ml lysis buffer
CK28L
2 x 20 s @ 6500
Human: breast cancer (Mammacarcinoma)
Protein
CK14
2 x 50 s 5000 rpm
Human: cancer of the colon

(30 300 mg)
Protein
300 l lysis buffer
CK14
2 x 30 s @ 6400 rpm
Human: colon/small intestine,

stomach
Protein
0,32 M sucrose buffer (min 400 l; 5

% tissue)
CK14
4 x [1 x 50 s @ 5000 rpm]
each 3 min pause on ice
Human: cornea
Protein
550 l PBS buffer, -Mercapto-EtOH,

SDS
CK14
4 x 23 s @ 6300 rpm
Human: liver, pericardium membrane, colon, (fine-cuts, freezedried)
Protein
Dry, cooled
CK28;
CK14S
1 x 20 s @ 6000 rpm
Human: lung
(3 x 1 cm)
Protein ?
5 ml RIPA buffer, satisfying homogenisation , with SKL better results
SKL;
CKL
2 x [ 2 x 30 s @ 6500]
Human: meninges
Native protein
PBS buffer
CK28L
2 x [1 x 30 s @ 6500 rpm]
pause on ice
Human: ovary cancer tissue

(1 mg)
Protein
30 l buffer
CK14
2 x 30 s @ 6500 rpm
30 s pause
Human: pancreas
Protein
CK14
2 x 50 s @ 5000 rpm
Human: skin cancer tissue

(100 mg)
Protein
500 l DNA-free water
CK28
3 x 30 s @ 6800 rpm
30 s pause
Human/bovine: sperm
(4 x 107 /sample)
Protein (nucleus)
in 250 l Heparin/DDT/Triton buffer
VK01
2 x 15 s @ 5000 rpm, 3 min

pause on ice
Human: tumor tissue (Meningioma) (100 mg)
Protein
1 ml homogenisation buffer [100mM

HEPES, pH7,4, 100 mM NaCl 0,5%
CK28
3 x 60 s @ 6500 rpm

Starting material
(amount)
Target molecule
(yield)
CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonic
acid)] per 100 mg tissue
200 l 5 x TBS + 100 l TritonX100 +
protease inhibitors; almost completely
homogenised
PCS-Kit
CKM
3 x 20 s @ 6800 rpm; 3 x
repeated
Human: umbilical
Protein
Human: liver
(pea-like piece)
Adenovirus
500 l H20; after centrifugation pos.

detection of the virus in the supernatant
CKM
2 x 20 s @ 5500 rpm
Human: placenta and kidney
Metabolites?
With interim cooling on dry ice satisfying results with MINILYS, better than
vortexing;
CK14
1 x 60 s @ 5000 rpm
Human: hair strains, 1- 2 cm

(20 mg)
MS, forensic analytic
Dry homogenisation, successful HPLCMS/MS
MK28
2 x 50 s @ 6500 rpm
15 s pause
Human: brain
(50 mg)
Fatty acids
350 l methanol (0C)
CK28
2 x 30 s @ 6000 rpm
Human: skin (with blisters)
NO, Nitrat, Nitrit
NEM (NEthylmaleimide)/EDTA/Trypsin
CK14
2 x [3 x 20 s @ 5500 rpm
30 s pause] with 3 min on ice
Cryolys level 1
CKM
2 x 20 s @ 5500 rpm
Human: colon and placenta

Krill (Euphausiacea)
(100 mg frozen abdominal
tissue)
Enzymes activity
In 1 ml ice-cold H2O; with Cryolys

combined; successful enzyme assays
CKM
2 x 15 s @ 5000 rpm
10 s pause
Leech: nerve cords
RNA
QIAzol lysis buffer
CK14
2 x 45 s @ 6500 rpm
Locusts
(20 mg)
DNA
(500 g) ??
Addition after homogensisation:

1 ml CTAB (acc. to Dumolin et al.,
1995)
CK14
1 x 15 s @ 5000 rpm
Lumbricus rubellus
Metabolites
1,1 g of 1 mm circonia beads in 2 ml

tube
CK01
2 x 10 s @ 6500 rpm
15 s Pause
Mammal: placenta
DNA
CK28
1 x 30 s @ 6000 rpm
Mammal: spleen
DNA
CK28
1 x 30 s @ 6000 rpm
Mammal: breast cancer tumor

and lymph node metastases
(frozen)
RNA
600 l RLT lysis buffer + mercaptoethanol
CK14
1 x 20 s @ 5500 rpm
Mammal: rumen
RNA
0.5 mL CTAB buffer and 0.5 mL

phenolchloroform3-methyl-1-butanol
(25:24:1) mixture
CK28
2 x 30 s @ 6200 rpm
Mammal: heart, spleen, pancreas
Virus RNA
Mammal: skin
RNA/protein
200 300 l buffer (partly with fluid

loss)
CK28;
CK28L
3 x 20 s @ 6200 rpm; 5 s
pause
Mammal: artery
Pharmaceut. substances
CK28
2 x 50 s @ 6000 rpm
Mammal: spinal cord
Protein, chem. substances
CK14
2 x 20 s @ 5500 rpm
CK14
brain: 2 x 30 s @ 6500 rpm,

lymph tiss.: 3 x 30 s @ 6500
rpm
15 s pause
CK14
CK28
skin: 2 - 4 x 30 s @ 6000 rpm

spleen: 1 x 30 s @ 6000 rpm
CK28L
1 x 40 s @ 5000 rpm
Mammal: brain, lymph tissue

(50 150 mg)
Prion protein
Mammal: skin (122 mg), spleen

(38 mg)
Bacteria (Streptococcus pyogenes)
Marten: faeces
(dry piece, approx. 2 x 2 cm
Metabolites
After homogenisation: 500 1500 l

5 % glucose sol.; successful analysis of
the Prion protein with ELISA and Western Blot
1 ml sterile NaCl buffer, viability of the
bacteria after homogenisation verified
by growth on agar plates
Homogenisation to powder without any
buffer, worked well in MINILYS

Starting material
(amount)
Target molecule
(yield)
Non-biting midge (Chironomus

riparius) larvae, adults
DNA
400 l 5 % Chelex (Sigma)
Mite (Psoroptes) of sheep: mixture of larvae, adults, nymphs
RNA
Monkey (Makaka): brain

(500 mg)
RNA
Monkey (Makaka): spleen, liver,

muscle, joints (30/100 mg)
Viral RNA
Monkey (chimpanzee): hair

(20 mg , pieces of 2 cm)
Steroids
Moth (Looper, Geometridae):

head and legs
DNA
Mouse: Aorta
DNA
Mouse: brain
(20 mg)
DNA
(15 g)
200 ml Lysis B. T + 20 l Prot K

(peqGOLD Tissue DNA Mini Kit)
Mouse: bones, thigh (femur)
DNA
dry, good quality
Mouse: ear
(20 mg)
DNA
(32 g)

CK14
2 x 20 s @ 5000 rpm
10 s pause
Mouse: fur with skin

(approx. 50 mg)
DNA & RNA
750 l peqGOLD TriFast or

350 l RLT-buffer(RNeasy, Qiagen)
CK14
3 x 15 s @ 6500 rpm
30 s pause
Mouse: heart
(20 mg)
DNA
(22 g)

CK14
1 x 15 s @ 5000 rpm
Mouse: liver
(20 mg)
DNA
(90 g)

CK14
1 x 15 s @ 5000 rpm
Mouse: lung
(20 mg)
DNA
(35 g)

CK14
1 x 15 s @ 5000 rpm
Mouse: lung, frozen

(15 mg)
DNA
500 l XTO buffer (Qiagen)
MK28R
1 x 15 s @ 6000 rpm + (1 x
15 s @ 5000 rpm)
Mouse: lung
DNA
4 ml buffer
CK28L
2 x 20 s @ 5000 rpm/ 2 x 20
s @ 6500 rpm; 10 s pause
Mouse: kidney
(20 mg)
DNA
(37 g)

CK14
1 x 15 s @ 5000 rpm
Mouse: muscle
(20 mg)
DNA
(9 g)

CK14
1 x 15 s @ 5000 rpm
Mouse: spinal cord

(10 mg)
DNA, RNA
200 l PBS; MINILYS, satisfying homogenate
CK14s
1 x 10 s @ 5000 rpm
Mouse: spleen
(20 mg)
DNA
(150 g

CK14
1 x 15 s @ 5000 rpm
Mouse: Aorta, testes, muscles
RNA
100 200 l lysis buffer, MINILYS,

satisfying homogenate
CK14S
1 x 20 s @ 5000 rpm
Mouse: arterial tissue

(30 mg)
RNA
(13 ng)
250 l TRIzol, nearly 2-fold higher

yield compared with competitor
CK14
2 x 10 s @ 6500 rpm + 1 x
15 s @ 6500 rpm, je 10 s
pause
Mouse: bones, Tibia

(100 mg)
RNA
MK28
8 x 10 s @ 6500 rpm, 20 s
pause on ice each cycle
Mouse: bones, tibia
RNA
Dry, frozen in N2, complete homogenisation, RNA quality comparable to

competitor devices
CK28
1 x 30 s @ 6000 rpm
PCS-Kit
VK05
2 x 30 s @ 6500 rpm
CK28
4 x 30 s @ 6200 rpm
5 ml TriFast and Tissue RNA lysis

buffer
CK28L
2 x 20 s @ 5500 rpm
15 s pause
1 ml lysis buffer for viral RNA

extraction (Macherey&Nagel); successful RT-PCR
CK28
MK28
2 x 10 s/20 s @ 5000 rpm
CK28R
2 x 30 s / 3 x 50 @ 6500
rpm
CK14
2 x 20 s @ 5000 rpm
CK28
2 x 30 s @ 5000 rpm
CK14
1 x 15 s @ 5000 rpm
100 l TE pH 8.0; peqGOLD Tissue

DNA Mini Kit
MK28 (R);
CK28
1 x 30 s @ 6000 rpm/1 x 20
s @ 6500 rpm

PCS-Kit
MK28R
3 x 30 s @ 6500 rpm
CK14
1 x 20 s @ 5000 rpm
RNA
300 l TriFast; macroscopically good

results
CK14S
1 x 10 s /20 s @ 5000 rpm
Mouse: cartilage
RNA
1 ml QIAzol
CK28
4 (6) x 15 s @ 6800 rpm
Mouse: cartilage (hyaline)

(100 mg)
RNA
Mouse: colon
RNA
500 l GTC-buffer
CK28
2 x 15 s @ 6800 rpm
30 s pause
Mouse: colon
(20 mg)
RNA
(6,4 g)
Precellys Tissue RNA Kit
CKM
1 x 20 s @ 5000 rpm
2 x 20 s @ 5500 rpm, 30 s
pause
Mouse: colon, frozen in N2

(1 piece : 8 to 10 mm length)
RNA
500 l peqGOLD TriFast
CK14
1 x 15 s @ 6600 rpm
Mouse: cornea
RNA
150 l RLT buffer + -Mercaptoethanol
CK14
3 x 20 s @ 6300 rpm
Mouse: ear
(50 mg)
RNA
(18 g)
CK14
2 x 20 s @ 5000 rpm
5 s pause
Mouse: inner ear bones, frozen

(1 piece)
RNA
500 l peqGOLD TriFast
CK28
2 x 20 s @ 5500 rpm
30 s pause
CK28
3 x 30 s @ 6800 rpm
15 s pause
Mouse: embryo, fixed in formalin RNA
Phenol-Chloroform extraction
CK14
CK28
2 x 20 s @ 6500 rpm
Mouse: heart
(50 mg)
RNA
(19 g)
CK14
1 x 20 s @ 5000 rpm
Mouse: kidney
(20 mg)
RNA
(approx. 25 g)
100 l RNA Lysis buffer T-P

CKM
1 x 20 s @ 5000 rpm
Mouse: kidney
(50 mg)
RNA
(23 g)
CK14
1 x 20 s @ 5000 rpm
Mouse: liver
(whole liver)
RNA
TRIzol, RNeasy
CK28L
1 x 15 20 s @ 5000 rpm
Mouse: liver
(30 mg)
RNA
350 l RA1 lysis buffer

(NucleoSpin RNA II Kit, M&N); (2-fold
yield compared to FastPrep 24)
CK14
2 x 20 s @ 6500 rpm
30 s pause
Mouse: liver
(50 mg)
RNA
(17 g)
CK14
1 x 20 s @ 5000 rpm
Mouse: lung
(50 mg)
RNA
(12 g)
CK14
1 x 20 s @ 5000 rpm
Mouse: lung, spleen, kidney
RNA
500 600 l lysis buffer, satisfying

homogenate, MINILYS
CK14
1 x 20 s @ 5000 rpm
Mouse: midriff (Diaphragma)
RNA
600 l buffer; approx. 3-fold higher

yield than with Retsch grinding mill
SK38
2 x 15 s @ 6500 rpm; 30 s
pause
Mouse: muscle
(50 mg)
RNA
(44 g)
CK14
1 x 20 s @ 5000 rpm
Mouse: pancreas
RNA
Saline buffer; Titration log CCID50/ml

Pancreas homogenate
CK14
CK28
3 x 5 s @ 5000 rpm
Mouse: pancreas
(30 75 mg)
Virus-RNA
Lysis buffer RLT (volume was adapted

to the amount of tissue to obtain a 5%
CK28
3 x 5 s @ 6500 rpm
Starting material
(amount)
Target molecule
(yield)
Mouse: bones
(50 mg)
RNA/Protein
Mouse: brain
(50 mg)
RNA
(12 g)
Mouse: brain
(5 mg)
Mouse: embryos, near to birth

1/2 embryo
RNA
MK28R
8 x 10 s @ 6500 rpm, 20 s
pause on ice each cycle

Starting material
(amount)
Target molecule
(yield)
PCS-Kit
w/v homogenate, successful quantitation of Virus-RNA

600 l RLT buffer, (RNeasy-Mini-Kit,
Qiagen); (good yield and cDNAsynthesis)
CK14
CK28
2 x 30 s @ 6500 rpm
20 s pause
RNA
(7 11 g/20 mg)
100 l RNA Lysis buffer T-P;
CKM
1 x 10 s @ 5000 rpm bis 2 x

15 s @ 5500 rpm
Mouse: skin, frozen in little

pieces
RNA or Protein?
With Cryolys cooling level 1
CKM
2 x 20 s @ 5500 rpm
5 s pause
Mouse: spleen, infected with

Listeria monocytogenes (20 mg)
RNA
RA1 buffer
CK14
1 x 30 s @ 6000 rpm
Mouse: uterus
RNA
RNeasy Mini Kit
CK28
2 x 20 s @ 6000 rpm
20 s pause
Mouse: testes
tRNA
1 ml TRIzol reagent, RT-PCR
CK14
2 x 20 s @ 6000 rpm
Mouse: tail
(50 mg)
RNA
(9 g)
CK14
3 x 20 s @ 6500 rpm
15 s pause
Mouse: embryonic tooth germs,

15-16 days post coitum
(0,75 mg)
RNA
200 l RLT lysis buffer + -MercaptoEtOH
CK14s
2 x 10 s @ 5500 rpm
10 s pause
Mouse: prostate gland

(15 mg)
Enzymes/RNA
1 ml TRIzol
CK14
1 x 20 s @ 5000 rpm
Mouse: lung, testes, brain
Enzymes/RNA
1 ml Proteinbuffer/TRIzol
CK14
1 2 x 20 s @ 6000 rpm
Mouse: Aorta
Protein
CK28
2 x 30 s @ 5000 rpm
Mouse: Aorta
Protein
200 l 5 x TBS + 100 l TritonX100 +

protease inhibitors; complete homogenisation
CKM
3 x 15 s @ 6500 rpm
Mouse: brain
(20 mg)
Protein
200 l buffer
CK14
1 x 20 s @ 5000 rpm
Mouse: brain, Hippocampus
Protein
CK14
2 x 30 s @ 5000 rpm
Mouse: brain ; Hemibrains,

frozen
Protein
CK14
2 x 30 s @ 6500 rpm
Mouse: brown adipose tissue

(30 50 mg)
Protein
CK28
2 x 50 s @ 5000 rpm
5 s pause
Mouse: cartilage
Protein
With and without lysis buffer
CK28 (R)
3 x 15 s @ 6800 rpm
Protein
1 ml 150 mM PBS with anti-protease

cocktail
CK14
1 x 60 s @ 6500 rpm
Mouse: eye
Protein
1 ml Ripa buffer
CKM
2 x 15 s @ 5000 rpm; 30 s
pause
Mouse: heart
Protein
1 ml RIPA-buffer
CKM
1 x 15 s @ 5000 rpm
2 x 15 s @ 5000 rpm; 30 s
pause
Mouse: heart
Protein
4-fold yield with MINILYS versus sonification; no degradation
CK14
1 x 20 s @ 5000 rpm
Mouse: heart
(40 60 mg)
Protein
(55 g/50 mg)
In Tris/HCL; SDS addition after homogenisation
CKM
10 s /15 s @ 5000 rpm
Mouse: skin
(30 mg)
RNA
Mouse: skin of sole of foot

(15 25 mg)
Mouse: ear, infected with Bacillus
anthracis
RIPA buffer with protease and phoshpatase inhibitors, successful protein

analysis
500 l of 10 mM Tris, pH 7.5, 100
mM NaCl, 5 mM EDTA, 0.5% Triton,
10% Glycerol
500 l 25 mM Tris/HCl, 1 mM EDTA;
1 % Triton X-100; 0,5 % Nadeoxycholate, successful Western blot

Starting material
(amount)
Target molecule
(yield)
Mouse: kidney
Protein
1 ml RIPA buffer
Mouse: kidney
Protein; mitochondria
shouldnt be destroyed
300 l buffer; complete homogenisation in CK28 ; in CK14 small pieces of

tissue remained
Mouse: liver
(100 mg)
Protein
Mouse: lung
(40 mg)
Protein
Mouse: lung
Protein
Mouse: pancreas
Native Protein
800 l PBS, incl. Cryolys cooling
CKM
Cryolys: 1 x 15 s @ 6000 rpm
Mouse: spleen, lung, colon
Protein
250 l RIPA buffer with 10 % protease

inhibitor (Sigma); very good results
with MINILYS
VK05
3 x 60 s @ 5000 rpm
Mouse: spleen
(20 mg)
Protein
RIPA-buffer with protease inhibitor

1:100 (Sigma 8340)
VK05
1 x 60 s @ 6000 rpm
Mouse: skin
Protein
700 l PBS
CK28
2 x 20 s @ 6200 rpm
20 s pause
Mouse: skin
(1-2 x 0,4 cm)
Protein
500 l PBS; equal results in FACS with

or without cooling
CKM (?)
2 x 30 s @ 6500 rpm; 20 s
pause
Mouse: sperm
Membrane protein,
partly native
Small volumes (200 l); better results

with buffer without Triton
CK14S
VK01S
2 x 20 s @ 5000 rpm
3 min pause on ice
Mouse: testes
Protein
CKM
1 x 30 s @ 5000 rpm
Mouse: tumors, subcutane autogenic

(approx. 100 mg)
Phoshorylated protein
CK28
1 x 30 s @ 6500 rpm
Mouse: tumor, (xenograft)
Natives Protein
CK28R
1 x 20 s @ 5000 rpm; 2 x 60
s @ 6000 rpm
1 ml PBS with defined germ number;

(nearly 100% recovery)
CK14
3 x 10 s @ 6800 rpm
15 s pause
Mouse and chicken: viscera

(200 mg)
Intact germs
Burkholderia thailandensis
PCS-Kit
CKM
1 x 15 s @ 5000 rpm
CK14; CK28
3 x 20 s @ 3000 rpm;
1 x 20 s @ 4000 rpm;
1 x 20 s @ 5000 rpm
1 ml PBS
CK14
1 x 25 s @ 5500 rpm
600 l RIPA-buffer
CKM
1 x 10 s @ 5000 rpm
CK28
MK28R
2 x 50 s @ 5000 rpm
1 ml detergent-containing buffer
with phosphatase inhibitor; very good
results: no dephosphorylation
With Cryolys temperature remained
ander 5C, better results in contrary to
grinding
Mouse: frontaler Cortex
Fatty acids
350 l methanol bei 0C
CK28
2 x 30 s @ 6000 rpm
Mouse: liver, dried
Fatty acids
500 l 2 % KCL
CK14
1 x 15 s @ 5000 rpm
Mouse: colon, liver, brain, lung

testes, partly complete
Stable chemical
elements, like Arsen
Almost complete homogenisation
CK28 L
2 x 20 s @ 6500 rpm
Mouse: adipose tissue

(200 mg)
Metabolites

buffer, with Cryolys cooling (level 1)
CK14S; CKM
2 x 20 s @ 5500 rpm
5 s pause
Mouse: brain
(200 mg)
Metabolites

CK14S; CKM 1 x 26 s @ 6500 rpm
Mouse: liver
(200 mg)
Metabolites

CK14S; CKM
Mouse: liver, kidney, muscle,

adipose tissue, brain (50 150
mg)
Metabolites
Methanol, 10 mM phosphat buffer, 6

l/mg liver + kidney; 3 l/mg other
tissue. Good, reproducible results in
High-Throughput tests
CK14
Mouse: kidney
(200 mg)
Metabolites

CK14S;
CKM
10
2 x 20 s @ 5500 rpm, 5 s
pause
3 x 20 s @ 5500 rpm
30 s pause
2 x 20 s @ 5000 rpm, 5 s
pause

Starting material
(amount)
Target molecule
(yield)
Mouse: muscle
(200 mg)
Metabolites

Mouse: heart (1/2)
Drugs
Concentration of specific drugs successfully measured
Mouse: lung
PCS-Kit
CK14S; CKM 1 x 20 s @ 6000 rpm

CK14
2 x 15 s @ 6500 rpm
Drugs
CK28
2 x 30 s @ 6000 rpm
Mouse: ovaries
(100 mg)
Drugs/HIV
CK28
2 x 25 s @ 5500 rpm
Mouse : feaces, frozen
Cortisol
10 vol 90 % ethanol, Cayman Corticosterone EIA Kit
CK14
3 x 20 s @ 5200 rpm bis 3 x

30 s @ 6500 rpm
Mouse: liver (100 mg), muscle

(165 mg)
Living cells
600 l PBS
3 beads
CK28
1 x 15 s @ 5000 rpm
Mouse: lymph nodes

(2 nodes = 8 mg)
Living cells
600 l PBS
3 beads
CK28
1 x 15 s @ 5000 rpm
Mouse: spleen
Living cells
CK28
2 x 20 s @ 5900 rpm
CK28R
3 x 30 s @ 6800 rpm; repeat

45x
Mouse: foetus, dried
Dry, good homogenisation
Mouse: testes
(120 mg)
1 ml buffer; good homogenisation
CKM
1 x 20 s @ 6500 rpm
Mouse: skeletal muscle & heart

(each 220 mg)
CK14
1 x 20 s @ 6000 rpm
Mouse: salivary glands
CK28
2 x 50 s @ 5000 rpm
Metabolites
4 ml /g (v/w) methanol + 0,85 ml

H2O, successful 1H NMR-spectroscopy
CK14
2 x 10 s @ 6400 rpm
30 s pause
DNA and RNA
1 ml TRIzol reagent
VK05
2 x 30 s @ 6800 rpm
Nematodes (Litomosoides sigmodontis) (3 - 5 female)
DNA and RNA
800 l TRIzol reagent
VK05
4 x 10 s @ 5000 rpm
Nematodes
Bacterial DNA
TRIzol reagent
MK28R
4 x 30 s @ 6800 rpm
Nematodes
(300 l suspension)
intact bacteria
300 l suspension
CK14; VK05
2 x 30 s @ 5000 rpm
10 s pause
Pig: ear
DNA
(approx. 7 g)
100 l TE-buffer + 25 l Prot K

Precellys Tissue DNA Kit
CKM
2 x 20 s @ 5000 rpm,
20 s pause
Pig: ear
(20 mg)
DNA
(approx. 20 g)

CK28
3 x 20 s @ 6500 rpm,
10 s pause
Pig: liver
(30 mg)
DNA
(90 g)
100 l TE-buffer + 25 l Prot K

Precellys Tissue DNA Kit
CKM
1 x 10 s @ 5000 rpm
Pig: liver
(20 mg)
DNA
(> 40 g)

CK14
1 x 5 s @ 5000 rpm
Pig: ear
RNA
(approx. 28 g)

CKM
2 x 20 s @ 5000 rpm
20 s pause
Pig: heart
(approx. 100 mg)
RNA
(high yield)
600 l RLT-buffer
RNeasy-Mini-Kit, Qiagen
CK28
2 x 20 s @ 6500 rpm
15 s pause
Pig: liver
(20 mg)
RNA
(approx. 32 g)

CKM
1 x 10 s @ 5000 rpm
Pig: bladder
(150 mg, frozen)
Protein
700 l PBS
CK28
2 x 25 s @ 6000 rpm
20 s pause
Mussels: gills
(100 mg)
Nematodes (Acanthocheilonema
viteae)
(2 female)
11

1 x 15 s @ 5000 rpm;
2 x 20 s @ 5500 rpm;
1 x 20 s @ 6500 rpm
Starting material
(amount)
Target molecule
(yield)
Pig: liver
(170 mg & 240 mg)
Protein
Sodium-calcium-phosphate-buffer
CK14
Pig: muscle
(100 mg)
Protein
0,5 ml TES buffer (10mM Tris ph 7,6;

1 mM EDTA and 0,25M sucrose)
CKM
2 x 20 s @ 6000 rpm
Pig: brain, kidney, liver, muscle

(1 g)
Drugs
4 volumes buffer pH 9
CK28L
2 x 30 s @ 6500 rpm
Pig: Iris ciliary body

(700 mg)
Drugs
2 volumes NaCl buffer; use of MINILYS
Pig: colon, frozen

(approx. 100 mg)
400 l PBS
PCS-Kit
CK28L + 2 4 x 30 s @ 5000 rpm

Kugeln CK50 15 s pause
CK14
1 x 30 s @ 6500 rpm
MK28R
2 x 20 s @ 6500 rpm
Pig (tea cup pig): skin
RNA
protein
Rabbit: gonads
(20 50 mg)
Lipids and Protein
Incl. Cryolys; higher yield compared to

grinding with mortar, little degradation
of lipids
CK28
MK28
2 x 15 s @ 6000 rpm
Rabbit: Retina
Drugs
1 ml buffer
CK28R
2 x [2 x 60 s @ 6800 rpm
Rat: bones
RNA
TriFast
Rat: liver
(30 mg)
RNA
(30-50 g)
600 l RLT buffer

(RNeasy-Mini-Kit, Qiagen)
Rat: shinbone (Tibia)

(70 and 150 mg)
RNA
500 l RLT buffer after 1. homogenisation; 126 g/l and 520 g/l,
respect.; good RealTime PCR
Rat: Soleus-muscle and Achilles

tendon
RNA
TRIreagent
CK28
5 x 20 s @ 6000 rpm
Rat: spleen
(approx.150 mg, ganz)
RNA
1 ml QIAzol Lysis reagent
CK14
3 x 20 s @ 6000 rpm
Rat: bladder
(200 mg)
Protein
1 ml protein buffer; use of MINILYS
CKM
2 - 3 x 30 s @ 5000 rpm
10 s pause
Rat: brain
(1/4 bzw 1/2 brain)
Protein
CK28L
1 2 x 15 s @ 5000 rpm
10 s pause
Rat: colon
(200 mg)
Protein
CKM
1 x 20 s @ 5000 rpm
Rat: lung
(100 mg/200 mg)
Protein
1 ml protein buffer, good results; use of

MINILYS
CKM
1 x 20 s @ 5000 rpm; 2 x 20
s @ 5000 rpm (200 mg), 10 s
pause
Rat & mouse: sperm
Protein (native)
500 l PBS, with Cryolys-cooling (level

1)
SK38
1 x 25 s @ 5500 rpm
Rat: heart
(1/2)
Drugs
H2O; successful measurement of both

drugs in dead tissue
CK14
2 x 15 s @ 6500 rpm
Rat: brain, muscle

(450 mg, 200 mg)
Drugs
2 Vol NaCL solution, use of MINILYS,

successful LC-MS/MS analysis
CKM
Brain: 2 x 45 s @ 5000 rpm

muscle: 3 x 40 s @ 5000 rpm
Rat: brain
(complete, approx. 1,7 g)
Drugs
2 Vol NaCL solution, use of MINILYS,

successful LC-MS/MS analysis
CK28L + 2
beads CK50
2 x 45 s @ 5000 rpm
Rat: brain
(entire, 1 g)
Drugs
4 ml 70 % Acetonitril
CK28L
2 x 10 s @ 5500 rpm
Rat: lung
(1,2 1,4 g)
Drugs/chemicals
3 Vol Acetonitril/0,9 % NaCL

(50/50v), successful analysis
CK28L
2 x 20 s @ 6500 rpm
Rat: liver, heart, kidney, testes,

adipose tissue (500 mg)
Phenol
1 ml ethanol
CK141
1 2 x 5 s, 10 s, oder 20 s @
6250 rpm
12
MK28; CK28
2 x 30 s @ 6500 rpm
15 s pause
CK14
2 x 10 s @ 5000 rpm,
10 s pause
MK28R
2 x 30 s @ 6500 rpm Dry; 30

s pause dann 1 x 30 s @
6500 rpm with buffer

Target molecule
(yield)
Rat: heart
(40 mg)
Enzyme activity
250 l HEPES 5mM, EGTA 1mM,

Triton 0.1%, DTT 1mM, pH 8.7, successful gemessen
CK14
2 x 8 s @ 6500 rpm
8 s pause
Rice fish (Medaka): liver, spleen,

skin, testes, embryos
RNA
500 l TriFast, RNA yield comparable

with Ultrathurrax
CK14;
CKM
2 x 20 s @ 5000 rpm; 10 s
pause
RNA
200 l TriFast
Sheep: bones & joints

(approx. 2 x 2 x 3 mm piece)
RNA
500 l RLT
CK28
2 x 20 s @ 6200 rpm,
15 s pause
Sheep: muscle & stomach

(50 mg)
RNA
500 l RLT
CK14
2 x 20 s @ 6200 rpm,
30 s pause
Sheep: teeth
(approx. 2 x 2 x 3 mm piece)
RNA
500 l RLT
MK28
2 x 20 s @ 6200 rpm,
15 s pause
Shellfish: ligaments
RNA
Qiagen RNA extraction kit
SK38
2 x 15 s @ 6000 rpm
Shellfish: gills
RNA
CK28
2 x 20 @ 6000 rpm
Shellfish: muscle
RNA
SK38
MK28R
2 x 20 s @ 6500 rpm
Sponge (sea-): Suberite domuncula olivi
Protein, enz. activity
Lysis buffer: 5 mM Tris, 0.05 M NaCl

and 0.001% Tween2O and 1% protease inhibitor
CK14
3 x 7 s @ 5600 rpm
30 s pause
Sponge (sea-): glass sponge
Biosilika
Dest. water
MK28R
8 x 25 s @ 6800 rpm
Shrimps
(100 mg)
DNA
(high yield)
500 l TE; good PCR results
CK28
2 x 30 s @ 6500 rpm
30 s pause
Tapeworm (Taenia)
RNA
CK14
3 x 20 s @ 6500 rpm
30 s pause
Tick
DNA
200 l lysis buffer with SDS
CKM
3 x 20 s @ 6500 rpm;
20 s pause
Tick
DNA (Rickettsia)
320 l minimum essential cell culture

medium; amplification of a fragment of
the gltA gen from rickettsia
MK28
2 x 30 s @ 5000 rpm
15 s Pause
Tick
RNA
MK28R
3 x 30 s @ 6000 rpm
Trematodes (parasites)
DNA
0,9 % NaCl buffer
Wale: muscle
DNA
CK28
2 x 20 s @ 6000 rpm
Wale: skin
DNA
CK14
SK38
2 x 20 s @ 6000 rpm
Wale: liver
RNA
CK14
2 x 15 s @ 5000 rpm
Water flea (Daphnia magna)
RNA
320 l methanol + 128 l H2O
CK14
2 x 10 s @ 6400 rpm
Water flea (Daphnia magna)

(entire, frozen)
Metabolites
Dry, after homogenisation a methanolH2O-chloroform mixture was added
CK14
2 x 10 s @ 6400 rpm
30 s pause
Zebra fish
RNA
VK05
3 x 10 s @ 6000 rpm
Zebra fish: larvae
Protein
CK14S
1 x 20 s @ 5000 rpm
Schistosoma (wurm-like parasite)

100 wurms ( 5 mm)
Starting material
(amount)
Homogenate with MINILYS was better

than using mortar and pistill
13
PCS-Kit
CK14s + 2
beads CK28
1-2 x 15 s @ 6500 rpm
3 x 30 s @ 6200 rpm
30 s pause

Starting material
(amount)
Target molecule
(yield)
Zebra fish: embryos

(Schmid Mnchen)
Protein
div. SDS-containing buffer; satisfying
homogenates, with Cryolys-cooling
(level 1)
14
PCS-Kit
VK05S;
CK14S
1 x 25 s @ 5500 rpm

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Precellys Application Database_Lysis protocols_Human & animal cells & tissues_v0413e

Successfully tested grinding parameters for

human and animal cells and tissues

Bat (Pteropus vampyrus): spleen

400 l Tissue DNA lysis buffer

0,8; 1,5; 4,5 ml TRIzol, partly with

5/15 s @ 6500 rpm

200 l Lysis B. T + 20 l Prot K

TriFast; good homogenisation

Bovine: colon, stored in RNAlater RNA

Bovine: kidney, lung

TriFast; good homogenisation

TriFast; good homogenisation

500 l RLT buffer

1 mL TES buffer (10 mM Tris (pH 7.6),

500 l 5 % glucose sol.

Bovine: probably muscle

350 L methanol (d 4 ) and 150 L

200 oder 500 l methanol-containing

Bug (Naucoris cimicoides)

300l lysis buffer (15% Sucrose, 0.05

Different lysis buffer

RNA with parts of

500 l M9-buffer, Qiagen RNA extraction, successful RT-PCR

Caenorhabditis elegans, fresh

500 ml 6 M Urea, 2 M Thiourea, 4 %

1 x 20 s @ 6000 rpm, mit

Precellys Application Database_Lysis protocols_Human & animal cells & tissues_v0413e

Human and animal cells and tissues

Cat: spleen, liver

200 L of a wasser/acetonitrile (1:1)

Cell culture: Hepatocytes (human) RNA, pharmaceut.

Cell culture (CH4, Hela, NB231)

Cell culture: PC12 cells

RIPA buffer with protease and phosphatase inhibitors, successful protein

Cell culture: human SW480-line

800 l Methanol/H2O (80:20)

Chicken: thigh (Tibia), skeletal

1 ml TriFast; successful RT-PCR

Chicken: Bursa fabricii

1 ml ice-cold 2D-DIGE lysis buffer/100

Crayfish: Abdomen, muscle,

Qiagen extraction kit

Drosophila: entire (adults)

Drosophila: entire (10 adults)

in 100 l TBS/0.1 % Triton and Protease inhibitor

Drosphila: entire (4 adults)

in 150 l buffer; good homogenisation;

Drosophila: Larven (8)

1mL 0.05% Tween-20

Drosophila: larvae (15) or heads

1 ml buffer, GS/MS analysis

Fish (eal): kidney and gills

50 mM Tris, 1 mM MgCl2 (pH 7.4)

Fish (cod): intestinal segments

1 ml Qiazol lysis buffer

Fish: pufferfish: muscle and liver

1 ml lysis reagent of RNeasy Mini Kit,

Fish (stickleback): liver

8 ml/g (v/w) methanol and 2,5 ml

Fish (salmon): muscle

1 ml extractionbuffer (100 mM Naacetate in 0,2 % Triton X-100, pH 5,5

Fish (salmon): colon

QIAzol lysis buffer