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DOI 10.1007/s00125-013-2899-8
ARTICLE
Received: 21 December 2012 / Accepted: 11 March 2013 / Published online: 26 April 2013
# Springer-Verlag Berlin Heidelberg 2013
Abstract
Aims/hypothesis Consumption of sugar-sweetened beverages
has been shown, largely in American populations, to increase
type 2 diabetes incidence. We aimed to evaluate the association
of consumption of sweet beverages (juices and nectars, sugarsweetened soft drinks and artificially sweetened soft drinks)
with type 2 diabetes incidence in European adults.
Methods We established a casecohort study including 12,403
incident type 2 diabetes cases and a stratified subcohort of
16,154 participants selected from eight European cohorts
participating in the European Prospective Investigation
into Cancer and Nutrition (EPIC) study. After exclusions, the
final sample size included 11,684 incident cases and a
subcohort of 15,374 participants. Cox proportional hazards
regression models (modified for the casecohort design)
and random-effects meta-analyses were used to estimate the
association between sweet beverage consumption (obtained
from validated dietary questionnaires) and type 2 diabetes
incidence.
Results In adjusted models, one 336 g (12 oz) daily increment
in sugar-sweetened and artificially sweetened soft drink
consumption was associated with HRs for type 2 diabetes of
1.22 (95% CI 1.09, 1.38) and 1.52 (95% CI 1.26, 1.83),
respectively. After further adjustment for energy intake and
BMI, the association of sugar-sweetened soft drinks with type
2 diabetes persisted (HR 1.18, 95% CI 1.06, 1.32), but the
The InterAct Consortium authors are listed in the Appendix.
Electronic supplementary material The online version of this article
(doi:10.1007/s00125-013-2899-8) contains peer-reviewed but unedited
supplementary material, which is available to authorised users.
The InterAct consortium (*)
c/o D. Romaguera, Department of Epidemiology & Biostatistics,
School of Public Health, Imperial College London,
St Marys Campus, Norfolk Place,
London W2 1PG, UK
e-mail: d.romaguera-bosch@imperial.ac.uk
Introduction
Use of caloric sweeteners (i.e. products used for sweetening
that are either derived from sugar crops, cereals, fruits or
milk) has increased around the world, with sugar-sweetened
beverages accounting for a major element of this increase
[1]. Parallel to this increase, there has been a rise in the
prevalence of obesity and type 2 diabetes worldwide [2].
Sugar-sweetened beverage consumption may lead to type 2
diabetes because of its effect on weight gain [3], as well as
its glycaemic effectsinducing rapid spikes in glucose and
insulin and causing insulin resistance [4, 5]. A meta-analysis
published in 2010 provides empirical evidence for a link
between the consumption of sugar-sweetened beverages
(defined as drinks containing energy sweeteners, such as
Methods
Study design The participants, methods, study design and
measurements have been described previously [16]. In brief,
the InterAct project was initiated to investigate how genetic
and lifestyle behavioural factors, particularly diet and physical
activity, interact for the risk of developing diabetes and how
knowledge about such interactions may be translated into
preventive action. As part of the wider InterAct project,
consortium partners established a casecohort study of
incident type 2 diabetes (InterAct study) based on cases
occurring within 3.99 million person-years accrued between
1991 and 2007 in 340,234 people from eight countries
participating in EPIC cohorts. All participants gave written
informed consent, and the study was approved by the local
ethics committees in the participating countries and the
Internal Review Board of the International Agency for
Research on Cancer.
Case ascertainment We followed a pragmatic highsensitivity approach for case ascertainment with the aim
of: (1) identifying all potential incident diabetes cases; and
(2) excluding all individuals with prevalent diabetes.
Prevalent diabetes was identified on the basis of baseline
self-report of a history of diabetes, doctor-diagnosed
1521
1522
Results
The distribution of different types of sweet beverage consumption by country/centre is shown in electronic supplementary material (ESM) Table 1. Overall, the average
consumption of sweet beverages was higher in northern than
in southern countries. Descriptive characteristics of the
population by categories of consumption are shown in Table 1
(total soft drinks) and in ESM Table 2 (juices and nectars),
ESM Table 3 (sugar-sweetened soft drinks) and ESM
Table 4 (artificially sweetened soft drinks). Compared with
low consumers, participants with high levels of total soft drink
consumption were more likely to be men, physically active,
less educated and smokers with, on average, a higher waist
circumference. The diet of high consumers of soft drinks was
relatively low in fruit and vegetables and rich in red and
1523
1524
Table 1 Baseline characteristics of the EPIC-InterAct subcohort (n=15,374) according to categories of total soft drink consumption (including
both sugar-sweetened and artificially sweetened soft drinks)
Characteristic
14 glassesa/month
>16 glassesa/week
1 glassa/day
n, %
Sex, % women
Physical activity, % activeb
Level of education, % university
Smoking status, % current
Cardiovascular disease, % yesc
Hypertension, % yes
Hyperlipidaemia, % yesd
Family history of type 2 diabetes, % yese
Age, years
8,349 (54.31)
65.48
16.86
20.71
25.22
4.10
19.27
19.74
19.70
52.93 (8.56)
2,034 (13.23)
62.49
21.48
22.71
26.16
4.24
17.62
18.14
18.28
52.21 (9.03)
3,765 (24.49)
57.90
24.44
20.45
25.79
5.66
18.17
16.12
19.10
51.69 (9.80)
1,226 (7.97)
52.37
25.77
16.31
30.42
6.35
20.41
16.86
18.22
51.70 (10.37)
BMI, kg/m2
Waist circumference, cmf
Alcohol, g/day [median (IQR)]
Juices, g/day
Soft drinks, g/day
Vegetables, g/day
Fruits, g/day
Cereals, g/day
Dairy product, g/day
Red meat, g/day
Processed meat, g/day
Sugar and confectionary, g/day
Cakes and biscuits, g/day
Coffee and tea, g/day
Energy, MJ/day
rMEDg
26.10 (4.18)
86.15 (12.64)
2 (13)
57.70 (118.66)
0.63 (1.75)
200.14 (126.85)
257.56 (198.01)
217.61 (114.67)
312.53 (221.16)
43.70 (35.27)
35.03 (31.82)
36.45 (46.46)
39.79 (45.72)
496.47 (483.23)
8.72 (2.58)
25.70 (3.99)
85.92 (12.38)
2 (13)
64.56 (119.56)
21.46 (7.36)
170.40 (106.44)
232.08 (189.36)
228.73 (122.88)
347.14 (239.47)
48.37 (35.56)
34.69 (30.11)
49.00 (54.52)
44.51 (45.27)
694.32 (518.09)
8.92 (2.61)
25.84 (4.14)
86.22 (12.44)
2 (13)
68.33 (109.31)
109.87 (57.59)
160.14 (103.69)
220.23 (174.49)
219.92 (116.28)
366.50 (254.62)
47.03 (36.21)
38.89 (32.65)
49.22 (50.56)
46.12 (44.23)
681.75 (464.04)
9.15 (2.70)
26.67 (4.54)
88.93 (13.52)
1 (12)
70.12 (113.30)
485.58 (276.28)
163.30 (111.94)
209.80 (169.80)
217.54 (109.97)
389.88 (292.67)
51.42 (39.14)
43.69 (36.68)
59.46 (73.29)
45.36 (47.21)
714.69 (503.61)
9.84 (2.82)
9.42 (3.04)
8.28 (3.04)
7.73 (2.93)
7.20 (2.86)
Active means classified in the category with the highest physical activity level according to the ordered four-category index of physical activity
(inactive, moderately inactive, moderately active, and active) [20]
c
Excludes Malm and Ume where information on hyperlipidaemia was not collected
Excludes all the centres in Italy, Spain, Germany and Oxford (UK) where family history of diabetes was not collected
1525
Table 2 HRs (and 95% CIs) for type 2 diabetes according to type and amount of sweet beverage consumption in the EPIC-InterAct study
Variable and model
<1 glassa/
month
HR
14 glassesa/
month
HRb (95% CI)
>16 glassesa/
week
HRb (95% CI)
1 glassa/
day
HRb (95% CI)
(0.0)
5,837
1.00 (ref)
1.00 (ref)
1.00 (ref)
1.00 (ref)
(0.0)
5,794
1.00 (ref)
1.00 (ref)
1.00 (ref)
1.00 (ref)
(0.0)
3,948
1.00 (ref)
1.00 (ref)
1.00 (ref)
1.00 (ref)
(0.0)
5,242
1.00 (ref)
1.00 (ref)
1.00 (ref)
1.00 (ref)
(17.1)
1,702
0.88 (0.80, 0.98)
0.91 (0.80, 1.02)
0.91 (0.81, 1.02)
0.97 (0.86, 1.10)
(20.0)
1,604
1.21 (1.07, 1.36)
1.21 (1.07, 1.37)
1.21 (1.07, 1.37)
1.17 (0.97, 1.42)
(19.3)
964
1.14 (0.97, 1.35)
1.13 (0.97, 1.31)
1.12 (0.96, 1.31)
1.19 (0.91, 1.56)
(18.3)
689
1.09 (0.97, 1.23)
1.10 (0.93, 1.29)
1.08 (0.93, 1.26)
1.05 (0.81, 1.35)
(100.0)
3,425
0.89 (0.83, 0.94)
0.96 (0.88, 1.04)
0.96 (0.88, 1.04)
1.04 (0.96, 1.13)
(95.1)
2,987
1.30 (1.18, 1.43)
1.26 (1.13, 1.42)
1.27 (1.12, 1.43)
1.11 (0.98, 1.26)
(94.3)
1,599
1.16 (1.05, 1.28)
1.04 (0.94, 1.15)
1.04 (0.94, 1.15)
1.07 (0.94, 1.21)
(89.0)
894
1.52 (1.36, 1.69)
1.46 (1.29, 1.65)
1.46 (1.29, 1.65)
1.18 (1.03, 1.35)
(338.3)
720
0.97 (0.85, 1.11)
1.00 (0.87, 1.15)
0.99 (0.86, 1.14)
1.06 (0.90, 1.25)
(413.1)
1,299
1.78 (1.55, 2.04)
1.58 (1.35, 1.84)
1.59 (1.35, 1.88)
1.21 (1.05, 1.41)
(425.7)
605
1.68 (1.40, 2.02)
1.39 (1.16, 1.67)
1.39 (1.15, 1.69)
1.29 (1.02, 1.63)
(500.0)
291
1.84 (1.52, 2.23)
1.93 (1.47, 2.54)
1.88 (1.44, 2.45)
1.13 (0.85, 1.52)
p for trend
0.64
0.63
0.84
0.21
<0.0001
<0.0001
<0.0001
0.0005
<0.0001
<0.0001
0.001
0.013
<0.0001
<0.0001
<0.0001
0.24
HRs from countries/centres by modified Cox proportional hazard regression; random-effect meta-analyses to obtain pooled estimates. Adjusted
models included sex, educational level, physical activity, smoking status and alcohol consumption; juices and total soft drinks were mutually
adjusted; sugar-sweetened and artificially sweetened soft drinks were also mutually adjusted plus adjustment for juice consumption
c
Total soft drinks include both sugar-sweetened and artificially sweetened soft drinks
Excludes Italy, Spain and Ume (Sweden) where information on type of soft drink consumption was not collected
Discussion
In European men and women, one 12 oz daily increment in
sugar-sweetened soft drink consumption was associated with
a 22% increase in HR for type 2 diabetes, and one 12 oz daily
increment in artificially sweetened soft drinks was associated
with a 52% increase in HR. After further adjustment for
BMI, the association between sugar-sweetened soft drink
<55 Years
55 Years
p for interaction
Sex
Men
Women
p for interaction
BMI
<25 kg/m2
2529.9 kg/m2
30 kg/m2
p for interaction
1.02
1.06
1.05
1.04
1.02
1.07
1.05
1.05
1.00
1.01
1.01
1.09
11,684
11,684
11,684
10,880
11,684
9,179
11,684
11,684
6,977
10,696
8,212
3,721
5,340
6,344
5,809
5,875
1,619
5,121
4,944
(0.94, 1.12)
(0.95, 1.18)
(0.94, 1.18)
(0.94, 1.16)
(0.93, 1.12)
(0.96, 1.18)
(0.96, 1.15)
(0.96, 1.15)
(0.88, 1.13)
(0.92, 1.11)
(0.89, 1.14)
(0.93, 1.26)
11,684
11,684
Unadjusted model
Adjusted model
Sensitivity analyses
+Energy intake
+BMI
+Energy intake+BMI
+Waist circumferenced
+Hypertension
+Hyperlipidaemiae
+Food groupsf
+rMEDg
Excluding baseline CVDh
Excluding first 2 years of follow-up
Excluding first 5 years of follow-up
Excluding family history of diabetesi
Effect modification
Age group
No. of cases
1.47)
1.26)
1.27)
1.29)
1.41)
1.55)
1.43)
1.45)
1.63)
1.46)
1.52)
1.52)
1.32 (1.19,
1.14 (1.04,
1.15 (1.05,
1.17 (1.06,
1.28 (1.16,
1.36 (1.20,
1.29 (1.16,
1.31 (1.18,
1.37 (1.16,
1.32 (1.20,
1.37 (1.23,
1.30 (1.12,
1,178
3,231
2,707
3,589
3,527
2,523
4,593
7,116
7,116
7,116
7,116
7,116
5,415
7,116
7,116
3,213
6,128
4,609
2,917
7,116
7,116
No. of
casesc
(1.08,
(1.07,
(1.06,
(1.05,
(1.06,
(1.09,
(1.05,
(1.06,
(1.03,
(1.09,
(1.10,
(1.03,
1.39)
1.30)
1.32)
1.32)
1.33)
1.50)
1.32)
1.35)
1.81)
1.34)
1.46)
1.56)
1.23
1.18
1.18
1.18
1.19
1.30
1.18
1.20
1.37
1.23
1.27
1.27
Table 3 HRs (and 95% CIs) for type 2 diabetes associated with one 336 g (12 oz) increment in the consumption of different type of sweet beverages in the EPIC-InterAct study, effect-modification
analyses and sensitivity analyses
1526
Diabetologia (2013) 56:15201530
Total soft drinks include both sugar-sweetened and artificially sweetened soft drinks
7,335
4,349
No. of cases
4,174
2,942
No. of
casesc
Excludes Malm and Ume (Sweden) where hyperlipidaemia was not collected
Excludes all cases from Italy, Spain and Ume (Sweden) where information on type of soft drink consumption was not collected (in addition to further exclusions as specified below)
The ordered four-category index of physical activity was combined into low (inactive and moderately inactive) and high (moderately active and active) physical activity
Excludes Italy, Spain, Heidelberg (Germany) and Oxford (UK) where family history of diabetes was not collected
History of cardiovascular disease at baseline: myocardial infarction, angina or stroke. Excludes the Netherlands, Heidelberg (Germany), Malm and Ume (Sweden) where information on angina
was not recorded
Consumption (in g/day) of vegetables, fruits, nuts, cereals and products, dairy products, red meat, processed meat, sugar and confectionary, cakes and biscuits, and coffee and tea as continuous
variables
HRs from countries/centres by modified Cox proportional hazard regression models; and random-effect meta-analyses to obtain pooled estimates. Adjusted models (also used in sensitivity and
effect-modification analyses) were adjusted for sex, educational level, physical activity, smoking status and alcohol consumption; juices and total soft drinks were mutually adjusted; sugar-sweetened
and artificially sweetened soft drinks were also mutually adjusted plus adjusted for juice consumption
Physical activityj
Low
High
p for interaction
Table 3 (continued)
1528
Appendix
The InterAct Consortium list of authors is as follows:
D. Romaguera (Department of Epidemiology and Biostatistics, School of Public Health, Imperial College London,
UK and CIBER de Fisiopatologa de la Obesidad y
Nutricin [CIBEROBN], Spain, www.ciberobn.es); T. Norat
(Department of Epidemiology and Biostatistics, School of
Public Health, Imperial College London, UK); P. A. Wark
(Department of Epidemiology and Biostatistics, School of
Public Health, Imperial College London, UK); A. C.
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1530
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