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CensusingPopulationsinAlgonquinPark

AppendixA.CountingPopulations
Thecensusingofplantandanimalpopulationsiscentraltopopulationecology,whichis
basicallythestudyofthepatternsoforganismabundanceanddistribution.Ecologistsseek
explanationsforwhyspeciessuchasBlackRat(Rattusrattus)canlivealmostanywhereand
areabundant,whentheKarnerBluebutterfly(Lycaeidesmelissasamuelis)israreandonly
foundinoaksavannahswithLupinbushes(Lupinusperennis).
Censusingapopulationisusuallyjustthestartofbroaderstudies,butitisnevertheless
importanttodoitright.Sincetotalanddirectcountsofpopulationsarerarelypossible(except
fortherarestofspecies),acensusentailsusingsamplingmethods.Thismeansthatweare
estimatingpopulationsizefromwhatwehopearerepresentativesamples.Byusingamethod
toenumerateapopulationtheobserverisinfluencingtheveryindividualstheyaretryingto
count.Howthemethodusedinfluencestheestimatesobtainedshouldbeunderstoodbythe
biologist.

A.1.SamplingMethods
Biologistsuseavarietyofsamplingmethodsdependingonthetypeoforganismanditsnatural
abundanceanddistribution.Inthisexerciseweuseavarietyofmethodswhichfallintotwo
broadcategories:
Plotbased(quadrat)methods,whereindividualsarecountedinaknownarea.Thistype
ofsamplingiswidelyused,especiallyforplants.
Capturebasedmethods,whichincludesmarkrecapture,andremoval.Thistypeof
samplingisusedforverymobileorelusivespecies.

A.2.QuadratSamplingMethod
Thequadratmethodhasbeenwidelyusedinplantstudies.Aquadratisafoursidedfigure
whichdelimitstheboundariesofasampleplot.Thetermquadratisusedmorewidelyto
includecircularplotsandothershapes.
Acommonshapeusedinstudiesofbirdsandlargemammalsisalong,skinnyquadratin
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whichtheobservertravelsatransectandcountsindividuals
withinaknownareaeithersideoftheline.Thesetypesof
quadratscanbecalledtransectstrips.(Therearevarious
distancebasedmethodswhichusetransectsandthedistanceoff
thelinethatindividualsareseen,butthesemethodsarenotdealt
withhere.)
Quadratsamplinginvolvescountingallindividualswithina
knownarea(orvolume).Sincedensity(D)andpopulationsize
(N)arerelated,asN=Dxarea,wecanestimatethedensityfor
thesampleandfromthiscomputethetotalpopulation.Thisassumesthattheareathe
populationoccupiesisfiniteandknown.InthecaseoftheAlgonquinParkspecies,wetakethe
Parkboundaries(inthecaseofMoose),theLakeOpeongoarea(LakeTrout),orthestudyarea
boundaries(WhitethroatedSparrow,SugarMaple)asthetotalarea.
Normally,aseriesofsamples(quadrats)arecounted.Wedonotknowwhich,ifany,ofthe
sampleestimatesgivethetrueestimate.Instead,wecancalculatetheaverageofthesesamples.
Theaverageormeanestimate,whilenotlikelytobethesameasthetruepopulationsize,is
neverthelessunbiased.Associatedwiththemean,wecancomputeameasureofthevariability
ofthesamples(thestandarddeviation),andthisisameasureofthereliabilityofourpopulation
estimate.

A.2.1.AssumptionsofQuadratSampling
Thequadratmethodhasthefollowingassumptions:
1. Thenumberofindividualsineachquadratiscounted.
2. Thesizeofthequadratsisknown.
3. Thequadratsamplesarerepresentativeofthestudyareaasawhole.

A.2.2.WorkedExample:EstimatingPopulationAbundancefromQuadrat
Samples
YouhaveusedMethodAtocountMooseinthreequadrats(each25squarekilometres)in
AlgonquinPark(totalarea=7,500squarekilometres).>Fromtheseresults,youwantto
determinebothameanestimateofthetotalMoosepopulationinthePark,andameasureofthe
reliabilityofyourestimate.Thefollowingcalculationsarerequired.
Note:Intheonlinesimulations,themeanandstandarddeviationarecomputedautomatically
foryouforeachmethod.

Step1:CalculateMeanPopulationAbundance
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Eachofthethreecountsisanobservation(n)andanestimateofthepopulationdensity(D.
WhenmultipliedbytotalParkarea,weobtainestimatesoftotalpopulationsize(N)inthe
Park.Sincewedonotknowwhichisclosesttothetruepopulationsize,wecancalculate
themeanofthethreeobservationswhichgivesusanunbiasedestimateofthetrue
populationabundance.
Table1.Samplecalculationusingquadratdata
Density
(Moose/25km2)

Count1

2100

Count2

11

3300

Count3

12

3600

TOTAL

9,000

Mean=(2100+3300+3600)3=3,000

Step2:ComputingtheStandardDeviation
Wenowwanttocalculateastatisticofthereliabilityofourmeanestimate.Wedothisby
measuringtheamountofscatterordeviationoftheobservations(counts)aroundthemean
estimate.Thisstatisticisthestandarddeviation.
Table2.Samplecalculationofstandarddeviation

Deviations
y=Nmean

y^2

sample1

2,100

2,1003,000=900

810,000

sample2

3,300

3,3003,000=300

90,000

sample3

3,600

3,6003,000=600

360,000

TOTAL

9,000

1,260,000

Thestatisticsthatarederivedfromthiscanbecalculatedasfollows:
Variance=1,260,0002=630,000
Standarddeviation=squarerootofthevariance=794
MoreinformationaboutthisandothermeasuresofdispersioncanbefoundinyourLab
Manual(Chapter3:IntroductiontoStatistics).

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A.3.MarkRecapture
Themarkrecapturemethodwasfirstusedinthe1890sbyC.G.Petersontoestimatefish
abundance.Todayitiswidelyusedinstudiesofmobileorganisms,suchasmammalsand
insects,andithasbeenappliedtohumanpopulationsaswell.
Themethodinvolvestakingasamplefromthepopulation,
markingthoseindividualsandreleasingthembackintothe
population.Aftertheindividualshavemixedfreelywith
unmarkedindividuals,newsamplesaretaken,andforeach
sampletheratioofmarkedtounmarkedindividualsisrecorded.
Thekeyprincipleofsamplingappliestothismethod:theratioof
unmarkedtomarkedindividualsinasamplewillbethesameas
theratiointhepopulationasawhole.
AsampleofManimalsiscollectedfromthepopulation,marked,thenreturnedtothe
population.Aftermarkedandunmarkedanimalshavemixed,asecondsampleofnanimalsis
capturedandthenumberofmarkedrecaptures,r,istallied.Weassumethattheratioofmarked
tounmarkedintherecapturesample,isrepresentativeoftheratioofmarkedtounmarkedin
thewholepopulation.HencewecanobtainanestimateofN.

A.3.1.Assumptions
Themarkrecapturemethodhasthefollowingassumptions:
1. Thepopulationisclosed(seeexplanationinboxedtextbelow).
2. Markedindividualshavethesameprobabilityofcaptureasunmarkedindividualsinthe
resamplingphase.
3. Markedandunmarkedindividualsmixrandomlybetweenthetimeofmarkingandthe
timeofresampling.
4. Marksarenotlostandarealwaysrecognizable.

ClosureofaPopulation
Closureofapopulationmeansthatitssizedoesnotchangeovertime.Apopulationisin
geographicclosurewhenthereareboundarieswhichlimittheindividualsspatially.An
exampleisalakepopulation.Apopulationisindemographicclosurewhenthereisnonet
changeinpopulationsizeduringthesamplingperiod(nobirths,immigration,emigration,
deaths).Inpractise,thismethodcanaccommodatelossesfromthepopulation,providedthe
ratethatmarkedandunmarkedindividualsleaveisthesame.However,inputsfrom
emigrationorbirthswillallbeofunmarkedindividualsandsowillbiastheestimate.

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A.3.2.WorkedExample:EstimatingPopulationAbundanceFromMark
Recapture
Assumethat50Moosehavebeenmarkedwithradiocollars(m=50)andreleasedtomixinthe
population.Later,yousamplethreeareasoftheParkandcounttheMoose(n)ofwhicha
subsetarerecaptures(r).Fromthesedatayouestimatepopulationsize,N.Herearesome
sampledataandhowpopulationsizeiscalculated.

CalculatingPopulationSizeFromMarkRecapture
Table3.Samplecalculationusingmarkrecapturedata

Count1

180

3,000

Count2

120

6,000

Count3

100

2,500

TOTAL

11,500

Meanpopulationsize=11,5003=3,833
Standarddeviation=1,893
Calculationofthemeanabundanceandstandarddeviationformarkrecapturesamplesuses
thesameprocedureasforquadratsamplingwhichisdescribedindetailabove.

2002UniversityofToronto.Allrightsreserved.
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