Beruflich Dokumente
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REVIEW ARTICLE
Keywords:
Dental
Stem
cells,
Regenerative Dentistry, Stem cells and
Dentistry,
Stem
Cells,
pulp
regeneration, Regeneration therapy.
Introduction
Corresponding Author
Rizwan Abdul Rasheed Shaikh,
Room no-105, First Floor, Boys Dental
Hostel, Govt. Medical College Campus,
Medical Square, Nagpur
PIN - 440 008
Email ID: rizsk123@gmail.com
Contact No. (+91)9561101768
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Abstract
The discovery of stem cells has developed
new but strange possibilities in the
regeneration of different tissue and organs.
Presently, research works and studies to
know potential and capabilities of stem cells
and their use in dentistry are becoming an
interesting topic. In a few years lots of
studies and demonstrations have been
carried out which show that stem cells and
tissue engineering are giving rise to a
separate branch named Regenerative
Dentistry that will have its own position in
future dental clinical practice. This short
review discusses the therapeutic potential of
stem cells in regenerative dentistry as seen
in studies and demonstrations carried out by
different workers.
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and also have the capabilities of generating
complex tissues and various organs [2, 24].
Stem cells were discovered on November 5,
1998 by workers at the University of
Wisconsin
(UWMadison)
and
Johns
Hopkins University (JHU). Embryonic stem
cells and postnatal stem cells are two types
of stem cells [2, 25-28]. During the process of
embryo development, at early stages,
embryonic stem cells are found in inner cells
of blastocyst [29]. Embryonic stem cells are
the main source of cells in regenerative
therapies because these cells have potential
of self-renewal and capability to generate
new tissues and organs. Embryonic stem
cells are totipotent , however due to legal
and ethical issues it is not feasible to use
embryonic stem cells in the laboratory and
clinics [30-32]. Post-natal stem cells can be
isolated from various tissues such as bone
marrow, neural tissue, skin, dental pulp,
and the periodontal ligaments [2]. Postnatal
stem cells also have the capabilities of self
renewal, however if we compare multipotent
postnatal stem cells with totipotent
embryonic stem cells then it is found that
postnatal stem cells have very limited
potential of differentiation into other types
of cells[1]. Main advantage of postnatal stem
cells is minimum risk of immune rejection
during tissue transplantation. Secondly,
these cells can be taken from donor
individuals at any stage of their life [33].
Non-Hematopoietic
Stem Cells
Mesenchymal
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24
(SHED) [28]. Dental Pulp Stem Cells (DPSCs)
is the name given to stem cells which are
present in Pulp tissue of human teeth [45]. In
the periodontal ligament researchers found
Periodontal Ligament Stem Cells (PDLSCs)
[71]. While in dental papilla of wisdom teeth
Stem Cells from Apical Papilla (SCAP) is
present[46,47] and researchers termed stem cell
found in dental follicles of developing wisdom
teeth as Dental Follicle Precursor Cells (
DFPCs ) [48-50].
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compared SHEDs with DPSCs and found that
SHEDs have a higher rate of proliferation [28].
Same as DPSCs, SHEDs also have the
potential to form bone that might be useful
during osseointegration of dental implants
coated with hydroxyapatite crystal and may
result in good levels of bone implant contact [21].
Seo et al. have demonstrated that SHEDs have
the potential to repair calvarial defects in
immunocompromised mice [67]. Studies shows
that SHEDs and DPSCs both have the
potential of generating tissues that have
similar
morphological
and
functional
characteristics resembling the human dental
pulp [62, 63, 68, 69].
Periodontal
(PDLSCS)
Ligament
Stem
Cells
Stem Cells
(SCAP)
from
Apical
Papilla
Dental Follicle
(DFPCs)
Precursor
Cells
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26
disintegrates into epithelial fragments and
allows contact between surface of dentin and
dental follicle ectomesenchymal cells, and
here these cells differentiate into mature
cells of the periodontium [76,77]. This
demonstrate that dental follicles contain
progenitor cells which have the capability of
differentiating into cementum forming cells
(cementoblasts), osteoblasts of the alveolar
bone, and periodontal ligament fibroblasts.
Handa K (2002) isolated progenitor cells from
bovine dental follicles. In in vitro conditions
these cells formed clusters of spheroid like
cells and in in vivo conditions, cementum
matrix formation took place by these cultured
dental follicle cells [78]. The human dental
follicle is a tissue which belongs to tooth
germ, and after wisdom tooth extraction one
can isolate these cells very easily.
Ectomesenchymal cells are present in the
dental follicles; these cells are derived from
the neural crest [54]. Similar to BMSCs,
DFPCs are colony forming cells which are
also plastic adherent. Under in vitro
conditions these cells can be differentiated
into osteoblast like cells [54]. Different workers
suggest that like PDLSCs, DFPCs can also
differentiate to produce mineralized tissue [48,
49, 79]. Salles and colleagues completed a study
which confirms that human DFPCs have
properties like mesenchymal precursor cells
[50].
DFSCs
can
differentiate
into
mesenchymal
derived
cells
like
cementoblasts, adipocytes and chondrocytes
[54].
Regenerative
Dentistry
by
giving
alternatives to root canal treatment (RCT)
and dental implants.
On the other hand, it is equally true that the
actual conditions to create the entire tooth
and tooth tissue in order to use it clinically
are not completely understood. More studies
and research works are required to use a
stem cell in making primordium tooth in vitro
for transplantation with the intention of
replacing a lost tooth. Hence the research
work on stem cells in regenerative dentistry
should be promoted to have its own position
in the clinical practice of dentistry in the
future.
Acknowledgements
I thank Dr. (Mrs.) W. A. Bhad, Head of
Department, Department of Orthodontics
and Dentofacial Orthopedics, Govt. Dental
College and Hospital, Nagpur for promoting
the undergraduate students in scientific and
research activities. I am also grateful to Dr.
Riyaz Ahemad, Post Graduate Student in
Oral and Maxillofacial Pathology and
Microbiology, Govt. Dental College and
Hospital,
Nagpur
for
his
valuable
suggestions and help, and for being a
constant source of inspiration during this
work
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