Code No: RR412310 Set No.

1
IV B.Tech I Semester Supplimentary Examinations, February 2008
SPECTROSCOPIC ANALYSIS OF BIOMOLECULES
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. (a) What is meant by dual nature of light?

(b) Define each of the following: absorption, emission, transmission, dispersion,
Scattering and refraction. [4+12]

2. (a) What are the factors which influences the positions of absorption frequencies
form their normal value?
(b) Write a note on fermi resonance. [8+8]

3. (a) Which crystals are used for optical system infrared instruments.
(b) Write the interpretation of IR for nucleic acids. [4+12]

4. What type of transitions are observed in α, β -unsaturated carbonyl compounds?

How absorption maximum and intensity are shifted when carbonyl group is not
conjugated? Discuss the effect of solvent polarity on R-band. [16]

5. (a) Discuss broadly about the role of metal ions in biological systems and their
study by using U.V.visible spectrophotometer.
(b) Write the structures of some important chromophores. [8+8]

6. (a) Write the principles involved in NMR Instrument.

(b) Discuss about spin-spin coupling.
(c) Write a brief note on flipping of the proton. [5+6+5]

7. (a) Predict the appearance of the high resolution NMR spectrum of 2- hydrox-
yproponoic acid.
(b) Explain the coupling constants and their applications. [8+8]

8. Describe the role of ESR in chemical biology. [16]

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Code No: RR412310 Set No. 2
IV B.Tech I Semester Supplimentary Examinations, February 2008
SPECTROSCOPIC ANALYSIS OF BIOMOLECULES
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) What is photoelectric effect? Discuss its theory

(b) What are photoelectric cells? Describe their construction and working. [8+8]

2. (a) Explain the solid sampling technique in IR spectrometer.

(b) Discuss the inductive and mesomeric effects influencing the carbonyl absorp-
tion frequency. Give examples. [6+10]

3. (a) Write the use of IR in the study of amines.

(b) For IR 5µm, what is the wave number in cm−1 and wave frequency in Hz.
[8+8]

4. (a) Detail the chemistry of electronic spectroscopy. Give the various types of
transitions involved in this technique with one example in each case.
(b) Explain the effect of polar solvents on [8+8]
i. n→ Π* and → Π* transitions.

5. Describe the role of U.V. visible spectrophotometer in diagnosis of biological sam-

ples. [16]

6. “NMR spectroscopy is most important tool compared to U.V and I.R. for the
structure determination of an unknown biomolecule.” Justify this statement. [16]

7. (a) Name some important solvents used in nmr spectroscopy . What are the
important features of the solvents used in this technique.
(b) The compound with MF = C9 H10 O2 in the 1 H NMR recorded in CDCl3 /TMS
showed signals at 1.2 (t,3H), 2.8(q,2H),7.7-7.8(m,5H). Deduce the structure of
the compound. [8+8]

8. (a) What is hyperfine splitting in ESR spectroscopy? Predict the number of ESR
spectral lines expected for CH3∗ and CD3∗ radicals. Explain the relative inten-
sities in each case.
(b) Write a small note on scale of EPR. [8+8]

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Code No: RR412310 Set No. 3
IV B.Tech I Semester Supplimentary Examinations, February 2008
SPECTROSCOPIC ANALYSIS OF BIOMOLECULES
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) What is meant by Compton effect? Explain its theory.

(b) What is the Compton wavelength of electron?
(c) Determine the maximum value of Compton shift. [8+4+4]

2. (a) Explain the different sampling techniques in IR.

(b) Illustrate various types of bending and stretching vibrations that can take
place in triatomic molecules. [8+8]

3. (a) Explain the different I.R peaks for the malonic acid and diethyl malonate.
(b) Explain the IR spectra for Proline and Phenyl alanine. [8+8]

4. “Increase in polarity of the solvent shifts Π → Π∗ band to longer wavelength but

n-Π∗ and n→ σ ∗ bands to shorter wavelength.” Comment on statement. [16]

5. Discuss the applications of UV Sspectroscopy in qualitative analysis and in quan-

titative analysis. [16]

6. Explain about peak area and proton counting in NMR spectra. [16]

7. Explain the NMR spectra (Signals, splitting patterns, Chemical shift values) for
the following compounds. [16]

(a) Steric acid (C17 H35 COOH)

(b) Dihydroxy acetone.

8. (a) Sketch the ESRspectrum arising from the interaction of an unpaired electron
with three equivalent protons.
(b) Explain the importance of ESRspectra in the study of Metallo proteins. [8+8]

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Code No: RR412310 Set No. 4
IV B.Tech I Semester Supplimentary Examinations, February 2008
SPECTROSCOPIC ANALYSIS OF BIOMOLECULES
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) Write short notes on different types of molecular energies.

(b) What is Zeeman and Starc effects? Discuss their theories. [8+8]

2. (a) Explain the electronic factors which influence the absorption frequency.
(b) How will you distinguish an aliphatic and an aromatic compound? [8+8]

3. (a) Which crystals are used for optical system infrared instruments.
(b) Write the interpretation of IR for nucleic acids. [4+12]

4. (a) Define electronic spectroscopy. What is its absorption range? Write the rela-
tionship between wavelength, frequency and wave number.
(b) Calculate the energy associated with the radiations having wave number 3 ×
104 per cm. [12+4]

5. (a) Explain about Fluorescence and Phosphorescence.

(b) Ultraviolet light of wavelength 180nm is incident on a tungsten surface. If the
wavelength of the photoelectric threshold of tungsten is 230 nm, determine
the energy of the photo-electrons ejected from the surface. [8+8]

6. Discuss different factors affecting the chemical shift. [16]

7. (a) Write the NMR signals and splitting pattern for L-Proline and Phenyl alanine.
(b) A pale yellow organic compound with molecular formula C6 H5 N O3 is found
to show two signals in the nmr spectrum 1.unsymmetrical patteren multiplet
= 7.1 - 8.2 δ (4H) 2. singlet = 0.9 δ. Describe the spectrum of the compound
and explain the position of each band. [8+8]

8. Describe the role of ESR spectra in the study of Bioinorganic molecules. [16]

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