Extraction of Omega-3s and

Phospholipids from Mussels

Using Supercritical Carbon Dioxide

Team 1
Chris, Dawydiak, Tamba Monrose, Robbie Shaw
March 12, 2014

Introduction
Background
Technical
Approach
Results
Discussion
Conclusion
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Technical
Approach
Safety
Conclusion

Use of Waste Mussel Meat

Waste mussels: mussels that are cracked, of wrong size or shape

Currently
Dumped in landfills

Alternatively
Extract oils for
supplements

Introduction
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Results
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Supercritical CO2 is a Popular Solvent

Characteris*c

Advantage

Nonpolar

Higher anity for organics than

water

Zero surface tension

Further penetra,on into matrices

Eec,ve mass transfer

Gas-like diusivi,es
Liquid-like densi,es

Manipula,on of temperature and

pressure

Easily altered solva,ng proper,es

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Preparing Mussels for Extraction

Taken from the Ocean

Removed from Shell and Dried

Ground & Crushed in Blender

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Mapping out Extraction System

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Loading the Sample

Extractor
Basket

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Loading the Sample

Porous
Medium
Basket
0.16 L

Mussel
Sample

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Liquid CO2 Enters System

Cold Heat
Exchanger

Water &
Ethylene Glycol
0 C
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Pumping CO2 up to sc-Pressure

Pneumatic
Pump

Compressed
Air
90 psi
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Heating CO2 up to sc-Temperature

Hot Heat
Exchanger

Water
70 C
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Extracting Oil
Needle
Valve

Extractor
Basket
Heater

Separator

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Introduction
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Heating CO2 and Oil and Entering Separator

Hot Heat
Exchanger
Water
70 C
Separator

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Venting Off CO2

Flow
Meter

Separator

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Continuously Flowing

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Collecting Oil Sample

Separator

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Designing Experiments
Varying
Pressure
Drying method of mussels
Flow Rate
Time
Temperature

Measuring
Mass

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Introduction
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We Struck Oil!
1) CO2 & Oil Enter

2) CO2 Leaves

3) Oil Leaves

Run Time = 1 hour

P = 200 bar
T = 60 C
Vacuum Oven Dried Mussels

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Introduction
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Discussion
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We Struck Oil!

Mussels Vacuum
and Oven Dried at
60C

Mussels
Conventionally
Dried at 90C

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Introduction
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Mass Results from Experiments

Theoretical Oil
L sc- flow
Mussels
Drying
Mussels Change Oil Content Collected CO2 rate Time Temp P
(bar) Method
(g)
(g)
used (L/hr) (hrs) (C)
Run Initial (g) (g)
Dried in
oven with
1
40.08
-4.22
4.81
0.8 0.28 0.16 1.7556-57
200 vacuum
Dried in
2
40.32
-1.33
4.84
- 0.72 0.25 2.8240-42
200 oven

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Introduction
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Discussion
Run 1 extracted more oil than Run 2.
Consider difference in mussels, temperature, and flow rate.

Oil must be collected extracted continuously.

Oil may be stuck in tubing.


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Introduction
Background
Technical
Approach
Safety
Conclusion
Introduction
Background
Technical
Approach
Results
Discussion
Conclusion

Supercritical CO2 will Extract Omega-3s and

Phospholipids
scCO2 is costly, safe, and effective solvent.
Oils can be extracted from waste mussel using
continuous scCO2 extraction.
Mussel drying method impacts oil extraction.

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Thank you to our...

Faculty Advisors: William Dalzell & Clark Colton
Technical Consultant: John Moses
Teaching Assistant: Whitney Loo
Communica,on Instructor: Amy Carleton
Team Coordinator: Bonnie Burrell von Stephoudt

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References
Carbon Dioxide: Pressure-Enthalpy Diagram, hZp://www.chemicalogic.com/Pages/
DownloadPhaseDiagrams.aspx

Dried Mussels, hZps://www.ickr.com/photos/95729516@N00/179349633/

Drawing of Mussel, hZp://clean-water.uwex.edu/pubs/clipart/criZers.riv.htm

Fish Oil, hZp://www.miamishoot.com/this-oil-has-more-nutrients-than-sh-oil/

Landll Mining, hZp://makewealthhistory.org/2013/03/13/scotland-inves,gates-
landll-mining/

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Questions?

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