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Introduction
When concentrations of solutes inside the cell differ from concentrations outside
of the cell, diffusion occurs. Solutes pass through the selectively permeable membrane in
order to create equilibrium between the two sides. Not every solute, however, can pass
through the membrane. Only small, nonpolar molecules pass through the membrane
readily.
Diffusion of water is called osmosis. Osmosis depends on factors such as
temperature and concentration (Freeman 2014). This investigation used dialysis tubing to
mimic the selectively permeable membrane of the cell. This investigation examined the
hypothesis that temperature directly correlates with rate of osmosis in a cell (Laboratory
Exercises for General Biology 1).
Fig. 3: The masses, measured every five minutes, of cells in various beakers.
Results were obtained by measuring the mass of each cell prior to submerging it
in a beaker, then measuring the mass of each after five minutes for twenty-five minutes.
The rate of osmosis was calculated with the following equation: (gfinalg
initial)/minutes.Forexample,thecontrolwascalculatedinthefollowingmanner:(5.1g
5.1g)/25minutes=0g/min.
Ineachcell,themassfluctuatedratherthanmerelyincreasingasexpected.In
thehotwater,forexample,themassdecreasedaftertenminutes,andagainafterfifteen,
beforeincreasingagain(seefig.1).Intheicewater,themassfluctuatedgreatly,butthis
wasduetoaleakinthecell.
Thegreatestrateofosmosiswasfoundintheroomtemperaturebeaker.
Discussion
The lab focused on the effect of temperature on the rate of osmosis. The
expectations were that temperature has a direct effect on osmosis with cold water
diffusing slower and hot water diffusing more rapidly. The hypothesis formed for the
experiment went unsupported by the data collected. According to Fig. 2, the room
temperature water had a higher rate of diffusion than the hot water, though were the
hypothesis supported, the hot water would have had the highest rate of diffusion. The
rates of osmosis were all precise, ranging from -0.004 g/ min to 0.008 g/ min. Thus,
based on the results, temperature had little to no effect that could be seen on the rate of
diffusion.
There were some sources of error including a leak in the ice water cell that could
have contributed to the failure of the hypothesis. Another source of error was that the
concentrations of the solutions inside and outside of the cells differed (the starch solution
was 2%, while the iodine solution was 1%). This may have affected the rate of osmosis.
Though the hypothesis went unsupported by the data, past studies on the effect of
temperature on osmosis support the hypothesis that higher temperatures increase the rate
of osmosis. In an article by the Department of Chemical Engineers at the University of
Bahrain, a positive relationship between temperature and rate of osmosis was proposed.
Al-Bastaki and Al-Qahtan studied salt passage through a semi-permeable membrane,
finding that an increase in temperature led to an increase in salt passage (Al-Bastaki NM,
Al-Qahtan, HI 1994). In a study by Magee, Hassaballah, and Murphy, it was proposed
that the ideal temperature for osmosis was 50 C (Magee TRA, Hassaballah AA, Murphy
WR, 1983).
The data gathered in the lab refutes the hypothesis that an increase in temperature
will lead to an increase in rate of osmosis. The room temperature water had a higher rate
of osmosis than the hot water. The ice water data could not be interpreted due to the leak
in the cell, which caused a negative rate of osmosis (see fig. 2). However, other studies
on the subject suggest that higher temperatures cause higher rates of osmosis.
References
Biology 281 Conceptual Approach Bio Majors 1. Laboratory Exercises for General
Biology 1. Plymouth: Hayden-McNeil Publishing, 2013. Print
Al-Bastaki, N.M., Al-Qahtan, H.I. 1994. Assessment of thermal effects on the reverse
osmosis of salt/water solutions by using a spiral wound polyamide membrane.
Desalination. Vol. 99, Issue 1. Pages 159 168.
Freeman, Scott, et al. Biological Science: Fifth Edition. 2014; Pearson. Page 92.
Magee, T.R.A., Hassaballah , A.A., Murphy, W.R. 1983. Internal Mass Transfer During
Osmotic Dehydration of Apple Slices in Sugar Solutions. Irish Journal of Food
Science and Technology. Volume 7, Issue 2. Pages 147 155.