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Examples
EC 4, Lyases:
Cleave various bonds by means other than hydrolysis and oxidation
EC 6, Ligases: join two molecules with covalent bonds with breakdown of the energy containing
substances.
Properties of enzymes
1. Enzyme is specialized protein
It exhibit all properties of proteins
2. Colloidal nature : Due to large size of molecules
3. Catalytic property
Enzymes are very efficient catalyst. Only small amount of enzymes is enough to convert large
quantity of substrate in product. It Speed up reaction by 106 - 1012 times greater than those of
the corresponding un catalyzed reactions
4. Specific
Enzymes are more specific toward their substrates and for the type of reactions that catalyze.
Enzymes shows 3 types of specificity
a. Specific specificity
c.
Inhibitors
Compounds which convert the enzymes into inactive substances and thus adversely affect the rate of
enzymatically-catalyzed reaction are called as enzyme inhibitors. Such a process is known as enzyme
inhibition.
1. Reversible inhibitors
A reversible inhibitor dissociates very rapidly from its target enzyme
A. Competitive inhibitors
Has a structure similar to substrate
Occupies active site
Competes with substrate for active site
Has effect reversed by increasing substrate concentration
For example presence of heavy metal like Ag+, Hg +2 etc inhibit the activity of a variety of enzymes.
Urease.
3. Irreversible inhibitors
Irreversible inhibitors are those that combine with or destroy a functional group on the enzyme that is
essential for its activity. For example iodoacetamide irreversibly inhibit the catalytic activity of some
enzymes by modifying cysteine.